>MLH1|NM_001167617|3'UTR
   1 ATATGGTTATTTATGCACTGTGGGATGTGTTCTTCTTTCTCTGTATTCCGATACAAAGTGTTGTATCAAAGTGTGATATA
  81 CAAAGTGTACCAACATAAGTGTTGGTAGCACTTAAGACTTATACTTGCCTTCTGATAGTATTCCTTTATACACAGTGGAT
 161 TGATTATAAATAAATAGATGTGTCTTAACATAAAAAAAAAAAAAAAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' ucggucgguUGAGUGUCACGUcu 5' |: || ||||:| Target 5' aagtgtgatATACAAAGTGTAcc 3'	69 - 91	114.00	-5.40
2	miRNA 3' ucggucGGUUGAGUGUCACGUCu 5' ||:|: || ||||: | Target 5' tgtattCCGATACAAAGTGTTGt 3'	42 - 64	113.00	-10.90
3	miRNA 3' ucggucgguUGAGUGUCACGUcu 5' |: ||||||| | Target 5' tattcctttATACACAGTGGAtt 3'	139 - 161	106.00	-10.56

Mutant ID	Mutant Position	Mutant Source
973410	1	ClinVar
921879	2	ClinVar
89586	4	ClinVar
388562	5	ClinVar
627657	7	ClinVar
631175	7	ClinVar
921177	7	ClinVar
924048	18	ClinVar
630433	19	ClinVar
918665	19	ClinVar
36549	30	ClinVar
135561	33	ClinVar
901875	122	ClinVar
344911	158	ClinVar
COSN30593451	19	COSMIC
COSN30490706	42	COSMIC
COSN26649668	44	COSMIC
COSN28883125	50	COSMIC
COSN30104230	62	COSMIC
COSN31601905	81	COSMIC
COSN31576802	88	COSMIC
COSN31530044	104	COSMIC
COSN31480313	135	COSMIC
COSN31599327	146	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs267607908	1	dbSNP
rs587778880	4	dbSNP
rs750794400	5	dbSNP
rs779336928	7	dbSNP
rs758561792	11	dbSNP
rs766650278	15	dbSNP
rs75682204	16	dbSNP
rs1046565300	18	dbSNP
rs1288659903	22	dbSNP
rs79406218	22	dbSNP
rs1192725744	23	dbSNP
rs1217831024	24	dbSNP
rs1388603455	26	dbSNP
rs892049206	28	dbSNP
rs1475224876	30	dbSNP
rs193922366	30	dbSNP
rs200903126	33	dbSNP
rs1375244357	34	dbSNP
rs781352547	40	dbSNP
rs772603751	42	dbSNP
rs954223482	42	dbSNP
rs749138642	43	dbSNP
rs778791265	46	dbSNP
rs745713835	50	dbSNP
rs1007307857	53	dbSNP
rs771845260	59	dbSNP
rs779892486	64	dbSNP
rs746506283	72	dbSNP
rs768003470	74	dbSNP
rs1017407837	77	dbSNP
rs1324446350	80	dbSNP
rs1299758105	82	dbSNP
rs1303794502	92	dbSNP
rs1311392464	94	dbSNP
rs776238530	96	dbSNP
rs1171427013	97	dbSNP
rs1803985	102	dbSNP
rs770302355	106	dbSNP
rs1208231152	107	dbSNP
rs773556906	110	dbSNP
rs1186418814	119	dbSNP
rs763379368	119	dbSNP
rs1194520604	120	dbSNP
rs766562208	122	dbSNP
rs751679614	123	dbSNP
rs963039575	128	dbSNP
rs1194759234	129	dbSNP
rs267607910	138	dbSNP
rs1485558939	140	dbSNP
rs1278445157	142	dbSNP
rs570963416	145	dbSNP
rs770660847	150	dbSNP
rs973488008	153	dbSNP
rs919154572	157	dbSNP
rs1336173991	158	dbSNP
rs796807655	158	dbSNP
rs201866587	163	dbSNP
rs538203686	166	dbSNP
rs56329536	166	dbSNP
rs917065097	166	dbSNP
rs587778879	168	dbSNP
rs1280032506	173	dbSNP
rs1400366298	174	dbSNP
rs1354654703	176	dbSNP
rs1300677285	185	dbSNP
rs1449836937	187	dbSNP
rs556614001	191	dbSNP
rs368443548	192	dbSNP
rs1424085679	194	dbSNP

- Nature, 2013

Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.