pre-miRNA Information
pre-miRNA hsa-mir-569   
Genomic Coordinates chr3: 171106664 - 171106759
Synonyms MIRN569, hsa-mir-569, MIR569
Description Homo sapiens miR-569 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-569
Sequence 61| AGUUAAUGAAUCCUGGAAAGU |81
Evidence Experimental
Experiments SAGE
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
COSN23021417 4 COSMIC
COSN1083370 8 COSMIC
COSN30455038 12 COSMIC
COSN24300323 13 COSMIC
COSN32068274 16 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs752673293 5 dbSNP
rs1218920077 8 dbSNP
rs745722313 12 dbSNP
rs1368837885 14 dbSNP
Putative Targets

Gene Information
Gene Symbol OLAH   
Synonyms AURA1, SAST, THEDC1
Description oleoyl-ACP hydrolase
Transcript NM_001039702   
Other Transcripts NM_018324   
Expression
Putative miRNA Targets on OLAH
3'UTR of OLAH
(miRNA target sites are highlighted)
>OLAH|NM_001039702|3'UTR
   1 ATATTTTCCCTTTCACTTTTAAAATAATCAAAGTAATATCATACTCTTCTCAGTTATTCAGATATAGCTCAGTTTTATTC
  81 AGATTGGAAATTACACATTTTCTACTGTCAGGGAGATTCGTTACATAAATATATTTACGTATCTGGGGACAAAGGTCAAG
 161 CCAGTAAAGAATACTTCTGGCAGCACTTTGGGAGGCCAAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCGTCCTGG
 241 CTAACACCGTGAAACCCCATCTCTACTAAAAATACACAAAATTAGCCGGGCGTGGTGGTGGGCACCTGTAGTCCCAGCTA
 321 CTCGGGAGGCTGAGGCAGGAGAATGGTGTGAACCTGGGAGGTGGAGCTTGCAGTGAACCGAGATCGCTCCACTGCACTCC
 401 AGCCTGGGTGACAGATCCAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAATACTTCTGGCAGAGTCTTTTATCTTCCTAT
 481 TAAAATCTCACTTGATTCTCCTTTATGGGAAGTTTGTCGACAAAATTCATGATTAGTAAATTATCCATTTTTTCCTTCAG
 561 TTAGTTTAATGGTGAAGATGATTAACAGGGGAAATGCTTGAAGTAAATGATTGTTTCAATGGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' ugaAAGGUC---CUAAGUAAUuga 5'
             | :|||   |||||:|||   
Target 5' tacTGTCAGGGAGATTCGTTAcat 3'
103 - 126 110.00 -11.12
2
miRNA  3' ugaaaggUCC---UAAGUAAUUga 5'
                 |||   |  || |||  
Target 5' gggacaaAGGTCAAGCCAGTAAag 3'
146 - 169 90.00 -5.96
3
miRNA  3' ugAAAGGUCCUAAGUAAUUga 5'
            || :|||   |:||| |  
Target 5' tcTTCTCAG---TTATTCAga 3'
45 - 62 88.00 -7.06
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30489379 9 COSMIC
COSN30119318 10 COSMIC
COSN30167040 10 COSMIC
COSN30476659 10 COSMIC
COSN30498091 11 COSMIC
COSN16552495 15 COSMIC
COSN30465627 47 COSMIC
COSN21222407 54 COSMIC
COSN31574162 120 COSMIC
COSN30534821 153 COSMIC
COSN20363350 363 COSMIC
COSN24199356 610 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1481872531 1 dbSNP
rs752265045 3 dbSNP
rs757795733 4 dbSNP
rs112418351 5 dbSNP
rs368273145 7 dbSNP
rs746206315 9 dbSNP
rs777796204 11 dbSNP
rs770181850 14 dbSNP
rs775684071 16 dbSNP
rs1487218671 17 dbSNP
rs751431602 26 dbSNP
rs749568990 31 dbSNP
rs372523092 38 dbSNP
rs1302932894 40 dbSNP
rs774494401 47 dbSNP
rs757135545 48 dbSNP
rs781131267 49 dbSNP
rs77067865 50 dbSNP
rs1227270502 52 dbSNP
rs1356590489 55 dbSNP
rs1435538627 57 dbSNP
rs901071711 62 dbSNP
rs1217181161 66 dbSNP
rs115792249 71 dbSNP
rs888196973 76 dbSNP
rs1433445795 80 dbSNP
rs1006676354 85 dbSNP
rs1006636400 86 dbSNP
rs1307255850 98 dbSNP
rs1406074755 99 dbSNP
rs1016342729 106 dbSNP
rs1353575899 107 dbSNP
rs1477619990 120 dbSNP
rs532642733 121 dbSNP
rs1194140821 134 dbSNP
rs148267877 139 dbSNP
rs546818499 140 dbSNP
rs1220174075 142 dbSNP
rs534927849 143 dbSNP
rs1274513584 148 dbSNP
rs970884192 149 dbSNP
rs114542587 155 dbSNP
rs1279194255 156 dbSNP
rs568537287 159 dbSNP
rs141322592 170 dbSNP
rs1246917432 171 dbSNP
rs924199045 182 dbSNP
rs1437273883 186 dbSNP
rs1022123037 189 dbSNP
rs969432337 190 dbSNP
rs1176205633 196 dbSNP
rs1379210202 198 dbSNP
rs150802704 199 dbSNP
rs767186061 203 dbSNP
rs939327191 204 dbSNP
rs535698393 207 dbSNP
rs1422972011 208 dbSNP
rs1190487575 210 dbSNP
rs557106837 214 dbSNP
rs538417835 215 dbSNP
rs1176276078 218 dbSNP
rs1443432353 220 dbSNP
rs1329368103 223 dbSNP
rs539096868 228 dbSNP
rs889314512 229 dbSNP
rs942406047 233 dbSNP
rs185725238 234 dbSNP
rs71485544 235 dbSNP
rs780452199 243 dbSNP
rs754223039 245 dbSNP
rs1038133612 248 dbSNP
rs558312045 249 dbSNP
rs572470516 250 dbSNP
rs892380099 251 dbSNP
rs369902691 257 dbSNP
rs1031204672 259 dbSNP
rs145268129 260 dbSNP
rs202218155 261 dbSNP
rs989141374 261 dbSNP
rs969171408 264 dbSNP
rs1239518349 269 dbSNP
rs1163589438 276 dbSNP
rs561450171 277 dbSNP
rs1226776912 278 dbSNP
rs1463886543 279 dbSNP
rs1378762543 286 dbSNP
rs7917664 288 dbSNP
rs929759458 289 dbSNP
rs961080377 292 dbSNP
rs543698729 293 dbSNP
rs1421645662 296 dbSNP
rs919287734 297 dbSNP
rs557980742 300 dbSNP
rs985062375 305 dbSNP
rs1261491559 307 dbSNP
rs910824031 308 dbSNP
rs943486736 314 dbSNP
rs1040570580 318 dbSNP
rs1262529959 321 dbSNP
rs1217189479 322 dbSNP
rs900883697 324 dbSNP
rs190938250 325 dbSNP
rs1052098721 326 dbSNP
rs948083004 331 dbSNP
rs183131004 335 dbSNP
rs1010590046 336 dbSNP
rs1022139506 342 dbSNP
rs1188106603 344 dbSNP
rs905127622 347 dbSNP
rs1001955222 348 dbSNP
rs7902459 352 dbSNP
rs1455972998 354 dbSNP
rs960816773 356 dbSNP
rs7081383 363 dbSNP
rs572035250 364 dbSNP
rs1193439382 368 dbSNP
rs952116021 370 dbSNP
rs548543187 374 dbSNP
rs1466378312 378 dbSNP
rs984800929 380 dbSNP
rs568300116 381 dbSNP
rs1414010398 385 dbSNP
rs967625023 386 dbSNP
rs530826917 387 dbSNP
rs1398530696 389 dbSNP
rs976244696 389 dbSNP
rs1217826259 395 dbSNP
rs1326927223 398 dbSNP
rs1278039035 400 dbSNP
rs923467368 410 dbSNP
rs1326025758 411 dbSNP
rs1229213994 417 dbSNP
rs1253902933 419 dbSNP
rs1170384061 426 dbSNP
rs550502344 427 dbSNP
rs1462869016 430 dbSNP
rs1491458902 430 dbSNP
rs11292797 431 dbSNP
rs1195611015 431 dbSNP
rs1197290124 431 dbSNP
rs1339905275 431 dbSNP
rs1421431735 431 dbSNP
rs1491550503 431 dbSNP
rs199827897 431 dbSNP
rs397847013 431 dbSNP
rs760962109 431 dbSNP
rs771095293 431 dbSNP
rs1200535905 442 dbSNP
rs1252873209 444 dbSNP
rs1282302767 450 dbSNP
rs1303467142 451 dbSNP
rs1487298871 451 dbSNP
rs933742466 452 dbSNP
rs1192632655 453 dbSNP
rs1263702230 455 dbSNP
rs1430109876 467 dbSNP
rs976421341 471 dbSNP
rs11259458 473 dbSNP
rs1171212785 474 dbSNP
rs1465943929 481 dbSNP
rs1465559674 489 dbSNP
rs4748140 492 dbSNP
rs1377794986 495 dbSNP
rs1182714404 499 dbSNP
rs946352438 501 dbSNP
rs1418449972 505 dbSNP
rs552620432 506 dbSNP
rs909690515 512 dbSNP
rs1361513552 513 dbSNP
rs145445602 519 dbSNP
rs905117884 520 dbSNP
rs1239254082 530 dbSNP
rs1330158756 531 dbSNP
rs188222843 532 dbSNP
rs991776517 532 dbSNP
rs915839284 541 dbSNP
rs555332312 544 dbSNP
rs1376576213 545 dbSNP
rs1315436195 546 dbSNP
rs1305047826 547 dbSNP
rs1002112386 555 dbSNP
rs1351394117 556 dbSNP
rs1046420232 561 dbSNP
rs1366993216 564 dbSNP
rs775216464 572 dbSNP
rs1474797647 582 dbSNP
rs935446408 589 dbSNP
rs575077700 593 dbSNP
rs1375751793 595 dbSNP
rs114525213 597 dbSNP
rs557599376 598 dbSNP
rs952169710 623 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions hESCs (WA-09)
Disease 55301.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in SRR359787. RNA binding protein: AGO2. Condition:4-thiouridine ...

- Lipchina I; Elkabetz Y; Hafner M; Sheridan et al., 2011, Genes & development.

Article - Lipchina I; Elkabetz Y; Hafner M; Sheridan et al.
- Genes & development, 2011
MicroRNAs are important regulators in many cellular processes, including stem cell self-renewal. Recent studies demonstrated their function as pluripotency factors with the capacity for somatic cell reprogramming. However, their role in human embryonic stem (ES) cells (hESCs) remains poorly understood, partially due to the lack of genome-wide strategies to identify their targets. Here, we performed comprehensive microRNA profiling in hESCs and in purified neural and mesenchymal derivatives. Using a combination of AGO cross-linking and microRNA perturbation experiments, together with computational prediction, we identified the targets of the miR-302/367 cluster, the most abundant microRNAs in hESCs. Functional studies identified novel roles of miR-302/367 in maintaining pluripotency and regulating hESC differentiation. We show that in addition to its role in TGF-beta signaling, miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1. This study broadens our understanding of microRNA function in hESCs and is a valuable resource for future studies in this area.
LinkOut: [PMID: 22012620]
CLIP-seq Support 1 for dataset SRR359787
Method / RBP PAR-CLIP / AGO2
Cell line / Condition hESCs (WA-09) / 4-thiouridine, RNase T1
Location of target site ENST00000378228.3 | 3UTR | AGGGGAAAUGCUUGAAGUAA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 22012620 / SRX103431
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
GSE38226 Liver fibrosis 0.501 1.0e-2 0.458 1.8e-2 21 Click to see details
GSE28260 Renal cortex and medulla -0.459 5.7e-2 -0.319 1.4e-1 13 Click to see details
GSE17498 Multiple myeloma 0.135 2.0e-1 0.447 1.9e-3 40 Click to see details
GSE34608 Pulmonary tuberculosis and sarcoidosis -0.18 2.2e-1 -0.359 6.0e-2 20 Click to see details
GSE14794 Lymphoblastoid cells 0.071 2.5e-1 0.047 3.3e-1 90 Click to see details
GSE32688 Pancreatic cancer -0.106 2.8e-1 -0.126 2.5e-1 32 Click to see details
GSE26953 Aortic valvular endothelial cells 0.102 3.2e-1 0.219 1.5e-1 24 Click to see details
GSE38974 Chronic obstructive pulmonary disease -0.099 3.2e-1 0.124 2.8e-1 25 Click to see details
GSE21687 Ependynoma primary tumors 0.053 3.4e-1 0.030 4.1e-1 64 Click to see details
GSE17306 Multiple myeloma -0.048 3.7e-1 0.450 5.9e-4 49 Click to see details
GSE21849 B cell lymphoma -0.045 4.1e-1 0.298 5.8e-2 29 Click to see details
GSE27834 Pluripotent stem cells 0.051 4.3e-1 0.088 3.7e-1 16 Click to see details
GSE28544 Breast cancer -0.031 4.4e-1 -0.111 3.0e-1 24 Click to see details
GSE28544 Breast cancer -0.031 4.4e-1 -0.111 3.0e-1 24 Click to see details
GSE28544 Breast cancer -0.031 4.4e-1 -0.111 3.0e-1 24 Click to see details
GSE28544 Breast cancer -0.031 4.4e-1 -0.111 3.0e-1 24 Click to see details
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
58 hsa-miR-569 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004827 SPI1 Spi-1 proto-oncogene 2 1
MIRT086537 HSPE1-MOB4 HSPE1-MOB4 readthrough 2 2
MIRT086545 MOB4 MOB family member 4, phocein 2 2
MIRT154349 PARD6B par-6 family cell polarity regulator beta 2 4
MIRT182174 POU2F1 POU class 2 homeobox 1 2 2
MIRT190452 EIF5 eukaryotic translation initiation factor 5 2 2
MIRT191410 NAA30 N(alpha)-acetyltransferase 30, NatC catalytic subunit 2 2
MIRT199846 CSNK1G2 casein kinase 1 gamma 2 2 2
MIRT208376 SLC25A36 solute carrier family 25 member 36 2 2
MIRT275677 CARKD NAD(P)HX dehydratase 2 2
MIRT275764 TFDP1 transcription factor Dp-1 2 2
MIRT280470 UBR7 ubiquitin protein ligase E3 component n-recognin 7 (putative) 2 2
MIRT296592 FAM217B family with sequence similarity 217 member B 2 4
MIRT306847 FYTTD1 forty-two-three domain containing 1 2 2
MIRT443764 HLF HLF, PAR bZIP transcription factor 2 2
MIRT448026 GSR glutathione-disulfide reductase 2 2
MIRT448659 NCALD neurocalcin delta 2 2
MIRT449142 UQCRB ubiquinol-cytochrome c reductase binding protein 2 2
MIRT449225 RAD51B RAD51 paralog B 2 2
MIRT462421 TWISTNB TWIST neighbor 2 2
MIRT468478 SESN3 sestrin 3 2 4
MIRT478170 DENND5B DENN domain containing 5B 2 2
MIRT478577 VSIG2 V-set and immunoglobulin domain containing 2 2 2
MIRT481300 ATP5G3 ATP synthase, H+ transporting, mitochondrial Fo complex subunit C3 (subunit 9) 2 16
MIRT485894 ZFP36 ZFP36 ring finger protein 2 2
MIRT497242 FITM2 fat storage inducing transmembrane protein 2 2 2
MIRT500282 ZNF703 zinc finger protein 703 2 8
MIRT509536 RSBN1L round spermatid basic protein 1 like 2 4
MIRT511835 GPATCH8 G-patch domain containing 8 2 4
MIRT522437 MON1B MON1 homolog B, secretory trafficking associated 2 2
MIRT523538 GLO1 glyoxalase I 2 4
MIRT527631 MYBPC1 myosin binding protein C, slow type 2 4
MIRT528059 OLAH oleoyl-ACP hydrolase 2 2
MIRT532549 TXNL1 thioredoxin like 1 2 2
MIRT545503 NAP1L1 nucleosome assembly protein 1 like 1 2 2
MIRT546724 RNF6 ring finger protein 6 2 2
MIRT546737 RNF141 ring finger protein 141 2 2
MIRT550889 ACTA1 actin, alpha 1, skeletal muscle 2 2
MIRT554180 SLC35E2B solute carrier family 35 member E2B 2 2
MIRT555878 PAFAH1B2 platelet activating factor acetylhydrolase 1b catalytic subunit 2 2 2
MIRT558458 DCUN1D1 defective in cullin neddylation 1 domain containing 1 2 2
MIRT560375 TIMM8A translocase of inner mitochondrial membrane 8A 2 2
MIRT562672 AKT3 AKT serine/threonine kinase 3 2 2
MIRT563810 TUBA1B tubulin alpha 1b 2 2
MIRT564607 ZNF711 zinc finger protein 711 2 2
MIRT564881 YWHAE tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon 2 2
MIRT566724 MSL2 MSL complex subunit 2 2 2
MIRT566900 CSRP3 cysteine and glycine rich protein 3 2 2
MIRT570002 COL1A2 collagen type I alpha 2 chain 2 2
MIRT612300 ZFP14 ZFP14 zinc finger protein 2 2
MIRT618334 ZNF813 zinc finger protein 813 2 2
MIRT627658 RSBN1 round spermatid basic protein 1 2 2
MIRT630660 PLAG1 PLAG1 zinc finger 2 2
MIRT640802 MYCL1 MYCL proto-oncogene, bHLH transcription factor 1 1
MIRT651370 ZBTB21 zinc finger and BTB domain containing 21 2 2
MIRT709227 GPSM2 G protein signaling modulator 2 2 2
MIRT712058 ZBTB34 zinc finger and BTB domain containing 34 2 2
MIRT725006 ZPBP zona pellucida binding protein 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-569 Doxorubicin 31703 NSC123127 approved resistant High Breast Cancer cell line (MCF-7)
hsa-miR-569 Verapamil 2520 NSC272366 approved resistant High Breast Cancer cell line (MCF-7)
hsa-miR-569 Trametinib 11707110 NSC758246 approved sensitive High Melanoma cell line (M14) (50nM)
hsa-miR-569 Vemurafenib 42611257 NSC761431 approved sensitive High Melanoma cell line (M14) (50nM)
hsa-mir-569 Fluorouracil 3385 NSC19893 approved sensitive cell line (OE19)
hsa-mir-569 Cisplatin 5460033 NSC119875 approved sensitive cell line (BxPC3)
hsa-miR-569 Tamoxifen 2733525 NSC180973 approved resistant cell line (LCC2)
hsa-miR-569 Fluorouracil 3385 NSC19893 approved resistant cell line (KM12C) (72 h)
hsa-miR-569 Sunitinib 5329102 NSC750690 approved resistant tissue (CardB)
hsa-miR-569 Paclitaxel 36314 NSC125973 approved sensitive cell line (SKOV3)
hsa-miR-569 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-569 Cisplatin 5460033 NSC119875 approved sensitive cell line (A2780)
hsa-miR-569 Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide/Methotrexate/Gemcitabine resistant cell line (Bats-72)

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