pre-miRNA Information | |
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pre-miRNA | hsa-mir-4707 |
Genomic Coordinates | chr14: 22956950 - 22957029 |
Description | Homo sapiens miR-4707 stem-loop |
Comment | None |
RNA Secondary Structure |
Mature miRNA Information | ||||||||||||||||||||||||||||
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Mature miRNA | hsa-miR-4707-3p | |||||||||||||||||||||||||||
Sequence | 52| AGCCCGCCCCAGCCGAGGUUCU |73 | |||||||||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||||||||
Experiments | Illumina | |||||||||||||||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
miRNAs in Extracellular Vesicles |
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Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | DDTL | ||||||||||||||||||||
Synonyms | KB-226F1.2 | ||||||||||||||||||||
Description | D-dopachrome tautomerase like | ||||||||||||||||||||
Transcript | NM_001084393 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on DDTL | |||||||||||||||||||||
3'UTR of DDTL (miRNA target sites are highlighted) |
>DDTL|NM_001084393|3'UTR 1 GGATGAAGAAGAGGATTATGTGATCACAGGAATGTTGCATGCGGGATAATCCAAAGCTGGTTATCTCCAGGCCCTCACTC 81 TGCCAAGAGATCTCTCTGGAAGAAGCAGCCAGTTCACAGATGCCCTGGATCCCTCCGTGCCCAATCATAAAAAAGTCATG 161 ACCGTCCCTATCTTGCCAATCTGCCAGGACTCCAAGGGGAAAAAGCGGATAAGTATCCTTCAGGAGACAGAGAAAAAGAT 241 ATCATCAGCTCCTTGGCTAATACCACATCTTGCAAGACCCCTGCCAGGTACTCCCACTGTGGGTACTCAGGACAGCCTGC 321 CTCAGTCCACCAGGCATTTTGCAAACCTGCTCATCCCAATAATGATTTTCCCCAACCCCCAGCAGGGCAGTGGGACACTC 401 AGGTGTGGGAGGTAGCCGCACATCATGACCCAGCTAGGACAGACACACAATACAGTAGCCTCGGTGTGTGTGCCGTACAC 481 TCTATCATCTCCATCAGTTTGTGTACTGAGGGGTACCCTGCCTCCTAATTGCAGGCTCCCTAAGGGCACAGTACCTCAGC 561 CACCTGTTTTCTCAAAGGCTGAGCACAGGGTGACCCCCCACCCCACCAACCCCGCCACTAATGTCTGGGCCATAAGTGAA 641 CATGCCCCTCTCAGAGGTAACAGCAAGTTTCCAGTGAGGAAAACCAGAACTTGGGACAGCCTGGTTGGGGCGGGCGGGAG 721 TATGAACAGCCTGTCTTCTCATCATAAAATTGGCATAATGGAGATTATCTACCTCATGGGGATAGCATGTCTTGTCTGAG 801 CAAAGGGAGAGTGGATAGGGATTGTAAGGATCAAATGGGATCATGAGTGTAAAGTACCTGTAGAGGACCTAGCACATGGT 881 AAGTGTATAACACTTGTTGTTAGAGTAACAGTTTTCCCTGAGTATCCGTGGGAGATTTGTTCTAGAATCCCCAGAGGAAA 961 CCAGAATCTACATTTGCTCAAGTCCCTTACATAAAATGGTGTAATATTTGCATGTAACCTACACACATCCTCTCATATAC 1041 TTTAAATAATCTCTGGGTTACTTATAATACCTAATATAATGTACGTACTATGTAAATAGTTGTATTGTTTAGGGAATAAT 1121 GACAAGGAATAAAGTCTATACGTATTTTCAGTAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | hESCs (WA-09) | ||||||
Disease | 100037417.0 | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in SRR359787. RNA binding protein: AGO2. Condition:4-thiouridine
... - Lipchina I; Elkabetz Y; Hafner M; Sheridan et al., 2011, Genes & development. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Lipchina I; Elkabetz Y; Hafner M; Sheridan et al. - Genes & development, 2011
MicroRNAs are important regulators in many cellular processes, including stem cell self-renewal. Recent studies demonstrated their function as pluripotency factors with the capacity for somatic cell reprogramming. However, their role in human embryonic stem (ES) cells (hESCs) remains poorly understood, partially due to the lack of genome-wide strategies to identify their targets. Here, we performed comprehensive microRNA profiling in hESCs and in purified neural and mesenchymal derivatives. Using a combination of AGO cross-linking and microRNA perturbation experiments, together with computational prediction, we identified the targets of the miR-302/367 cluster, the most abundant microRNAs in hESCs. Functional studies identified novel roles of miR-302/367 in maintaining pluripotency and regulating hESC differentiation. We show that in addition to its role in TGF-beta signaling, miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1. This study broadens our understanding of microRNA function in hESCs and is a valuable resource for future studies in this area.
LinkOut: [PMID: 22012620]
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CLIP-seq Support 1 for dataset SRR359787 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | hESCs (WA-09) / 4-thiouridine, RNase T1 |
Location of target site | ENST00000215770.5 | 3UTR | GGUUGGGGCGGGCGGGA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 22012620 / SRX103431 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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91 hsa-miR-4707-3p Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT442514 | SYT7 | synaptotagmin 7 | 2 | 4 | ||||||||
MIRT457799 | NQO2 | N-ribosyldihydronicotinamide:quinone reductase 2 | 2 | 2 | ||||||||
MIRT466318 | THRA | thyroid hormone receptor, alpha | 2 | 2 | ||||||||
MIRT486129 | TRAF3 | TNF receptor associated factor 3 | 2 | 2 | ||||||||
MIRT492341 | SEPT8 | septin 8 | 2 | 2 | ||||||||
MIRT495690 | KCNC3 | potassium voltage-gated channel subfamily C member 3 | 2 | 2 | ||||||||
MIRT496928 | DPYSL5 | dihydropyrimidinase like 5 | 2 | 2 | ||||||||
MIRT499518 | MAFK | MAF bZIP transcription factor K | 2 | 2 | ||||||||
MIRT501367 | REXO1 | RNA exonuclease 1 homolog | 2 | 4 | ||||||||
MIRT501636 | PIAS4 | protein inhibitor of activated STAT 4 | 2 | 4 | ||||||||
MIRT521071 | SLC25A16 | solute carrier family 25 member 16 | 2 | 2 | ||||||||
MIRT525700 | PCYT2 | phosphate cytidylyltransferase 2, ethanolamine | 2 | 2 | ||||||||
MIRT532722 | DDTL | D-dopachrome tautomerase like | 2 | 2 | ||||||||
MIRT533137 | WWC1 | WW and C2 domain containing 1 | 2 | 4 | ||||||||
MIRT537288 | G3BP1 | G3BP stress granule assembly factor 1 | 2 | 4 | ||||||||
MIRT570555 | PHF21B | PHD finger protein 21B | 2 | 2 | ||||||||
MIRT573469 | MTRNR2L9 | MT-RNR2-like 9 | 2 | 2 | ||||||||
MIRT576750 | Tmem127 | transmembrane protein 127 | 2 | 2 | ||||||||
MIRT609342 | DAAM2 | dishevelled associated activator of morphogenesis 2 | 2 | 2 | ||||||||
MIRT610638 | PIGM | phosphatidylinositol glycan anchor biosynthesis class M | 2 | 2 | ||||||||
MIRT612591 | RANGAP1 | Ran GTPase activating protein 1 | 2 | 2 | ||||||||
MIRT614237 | WDR53 | WD repeat domain 53 | 2 | 4 | ||||||||
MIRT622784 | PGK1 | phosphoglycerate kinase 1 | 2 | 2 | ||||||||
MIRT628880 | MED16 | mediator complex subunit 16 | 2 | 2 | ||||||||
MIRT629550 | SPN | sialophorin | 2 | 2 | ||||||||
MIRT634714 | DDX19B | DEAD-box helicase 19B | 2 | 2 | ||||||||
MIRT634932 | GTF2H2C | GTF2H2 family member C | 2 | 4 | ||||||||
MIRT635341 | RBL1 | RB transcriptional corepressor like 1 | 2 | 2 | ||||||||
MIRT637838 | CPT1A | carnitine palmitoyltransferase 1A | 2 | 2 | ||||||||
MIRT644311 | NFKBID | NFKB inhibitor delta | 2 | 2 | ||||||||
MIRT649206 | KIAA1715 | lunapark, ER junction formation factor | 2 | 2 | ||||||||
MIRT657262 | HSPA4L | heat shock protein family A (Hsp70) member 4 like | 2 | 2 | ||||||||
MIRT658919 | DPY19L4 | dpy-19 like 4 | 2 | 2 | ||||||||
MIRT663608 | SEC23B | Sec23 homolog B, coat complex II component | 2 | 2 | ||||||||
MIRT667425 | MFAP2 | microfibril associated protein 2 | 2 | 2 | ||||||||
MIRT667667 | KREMEN1 | kringle containing transmembrane protein 1 | 2 | 2 | ||||||||
MIRT668144 | GDPD1 | glycerophosphodiester phosphodiesterase domain containing 1 | 2 | 2 | ||||||||
MIRT668540 | ERGIC1 | endoplasmic reticulum-golgi intermediate compartment 1 | 2 | 2 | ||||||||
MIRT670039 | NFRKB | nuclear factor related to kappaB binding protein | 2 | 2 | ||||||||
MIRT670875 | RAB10 | RAB10, member RAS oncogene family | 2 | 2 | ||||||||
MIRT672694 | ZNF677 | zinc finger protein 677 | 2 | 2 | ||||||||
MIRT673107 | MFSD2A | major facilitator superfamily domain containing 2A | 2 | 2 | ||||||||
MIRT673508 | TNFAIP8L1 | TNF alpha induced protein 8 like 1 | 2 | 4 | ||||||||
MIRT674244 | NUP62 | nucleoporin 62 | 2 | 4 | ||||||||
MIRT676541 | IRGQ | immunity related GTPase Q | 2 | 2 | ||||||||
MIRT676787 | CXorf38 | chromosome X open reading frame 38 | 2 | 4 | ||||||||
MIRT677600 | PRKX | protein kinase, X-linked | 2 | 2 | ||||||||
MIRT678607 | MRPL17 | mitochondrial ribosomal protein L17 | 2 | 2 | ||||||||
MIRT678940 | MYADM | myeloid associated differentiation marker | 2 | 2 | ||||||||
MIRT679104 | CD99 | CD99 molecule (Xg blood group) | 2 | 2 | ||||||||
MIRT679196 | WNT2B | Wnt family member 2B | 2 | 2 | ||||||||
MIRT679325 | ZMYM4 | zinc finger MYM-type containing 4 | 2 | 2 | ||||||||
MIRT679634 | BRMS1L | breast cancer metastasis-suppressor 1 like | 2 | 2 | ||||||||
MIRT679819 | TMEM106B | transmembrane protein 106B | 2 | 2 | ||||||||
MIRT679898 | FBXO48 | F-box protein 48 | 2 | 2 | ||||||||
MIRT680579 | ZNF784 | zinc finger protein 784 | 2 | 2 | ||||||||
MIRT680600 | SZT2 | SZT2, KICSTOR complex subunit | 2 | 2 | ||||||||
MIRT682991 | RNF40 | ring finger protein 40 | 2 | 4 | ||||||||
MIRT683483 | ZNF7 | zinc finger protein 7 | 2 | 2 | ||||||||
MIRT683681 | MICA | MHC class I polypeptide-related sequence A | 2 | 2 | ||||||||
MIRT684122 | CEP104 | centrosomal protein 104 | 2 | 2 | ||||||||
MIRT684774 | MYO1F | myosin IF | 2 | 2 | ||||||||
MIRT685698 | BHMT2 | betaine--homocysteine S-methyltransferase 2 | 2 | 2 | ||||||||
MIRT686471 | LINC00598 | long intergenic non-protein coding RNA 598 | 2 | 2 | ||||||||
MIRT687940 | HMGB1 | high mobility group box 1 | 2 | 2 | ||||||||
MIRT688627 | CRISPLD2 | cysteine rich secretory protein LCCL domain containing 2 | 2 | 2 | ||||||||
MIRT689110 | ZBTB25 | zinc finger and BTB domain containing 25 | 2 | 2 | ||||||||
MIRT690063 | MBD1 | methyl-CpG binding domain protein 1 | 2 | 2 | ||||||||
MIRT691067 | NUGGC | nuclear GTPase, germinal center associated | 2 | 2 | ||||||||
MIRT691317 | KIAA1841 | KIAA1841 | 2 | 2 | ||||||||
MIRT692778 | SYNPO2L | synaptopodin 2 like | 2 | 2 | ||||||||
MIRT694087 | KIAA0930 | KIAA0930 | 2 | 2 | ||||||||
MIRT696148 | WDR59 | WD repeat domain 59 | 2 | 2 | ||||||||
MIRT696176 | GNB5 | G protein subunit beta 5 | 2 | 2 | ||||||||
MIRT696434 | SUGP1 | SURP and G-patch domain containing 1 | 2 | 2 | ||||||||
MIRT697974 | TSPAN6 | tetraspanin 6 | 2 | 2 | ||||||||
MIRT698917 | SPEM1 | spermatid maturation 1 | 2 | 2 | ||||||||
MIRT699312 | SLC35F5 | solute carrier family 35 member F5 | 2 | 4 | ||||||||
MIRT700500 | PTPN4 | protein tyrosine phosphatase, non-receptor type 4 | 2 | 2 | ||||||||
MIRT700557 | PTBP1 | polypyrimidine tract binding protein 1 | 2 | 2 | ||||||||
MIRT701817 | MRPL37 | mitochondrial ribosomal protein L37 | 2 | 2 | ||||||||
MIRT702045 | METTL21A | methyltransferase like 21A | 2 | 2 | ||||||||
MIRT702215 | LPCAT1 | lysophosphatidylcholine acyltransferase 1 | 2 | 2 | ||||||||
MIRT702337 | KLHL7 | kelch like family member 7 | 2 | 2 | ||||||||
MIRT704139 | DNAL1 | dynein axonemal light chain 1 | 2 | 2 | ||||||||
MIRT704189 | LDHD | lactate dehydrogenase D | 2 | 2 | ||||||||
MIRT705344 | ATP1B3 | ATPase Na+/K+ transporting subunit beta 3 | 2 | 2 | ||||||||
MIRT706097 | ENTPD4 | ectonucleoside triphosphate diphosphohydrolase 4 | 2 | 2 | ||||||||
MIRT709068 | FAHD1 | fumarylacetoacetate hydrolase domain containing 1 | 2 | 2 | ||||||||
MIRT717579 | VTA1 | vesicle trafficking 1 | 2 | 2 | ||||||||
MIRT722440 | ZBTB7B | zinc finger and BTB domain containing 7B | 2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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