pre-miRNA Information | |
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pre-miRNA | hsa-mir-4753 |
Genomic Coordinates | chr1: 235190034 - 235190116 |
Description | Homo sapiens miR-4753 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | ||||||||||||||||||||||
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Mature miRNA | hsa-miR-4753-5p | |||||||||||||||||||||
Sequence | 10| CAAGGCCAAAGGAAGAGAACAG |31 | |||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||
Experiments | Illumina | |||||||||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||
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Gene Symbol | TAF1D | ||||||||||
Synonyms | JOSD3, RAFI41, TAF(I)41, TAFI41 | ||||||||||
Description | TATA-box binding protein associated factor, RNA polymerase I subunit D | ||||||||||
Transcript | NM_024116 | ||||||||||
Expression | |||||||||||
Putative miRNA Targets on TAF1D | |||||||||||
3'UTR of TAF1D (miRNA target sites are highlighted) |
>TAF1D|NM_024116|3'UTR 1 CAGGATCATGAATGTCAAAGGTGAAGCATATAGAAAAAACGACTTCATAGAAATGAATAAAGATAAATGTGGATATATGT 81 ACCAGTCTGGTGGTGAAGAAATTCTGAAACCCAGAACTTTATAACAAGAAAAAAAATTTTTAACCCTGTGAAGAAGTTTG 161 TGAAAGAAACTTGTGAAGTAGTAATAATTAGAAAAAAAACCATTAAAACACCAGAGAAAATACATAGAAAAAAAAAAAAA 241 AAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | hESCs (WA-09) |
Disease | 79101.0 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in SRR359787. RNA binding protein: AGO2. Condition:4-thiouridine
... - Lipchina I; Elkabetz Y; Hafner M; Sheridan et al., 2011, Genes & development. |
Article |
- Lipchina I; Elkabetz Y; Hafner M; Sheridan et al. - Genes & development, 2011
MicroRNAs are important regulators in many cellular processes, including stem cell self-renewal. Recent studies demonstrated their function as pluripotency factors with the capacity for somatic cell reprogramming. However, their role in human embryonic stem (ES) cells (hESCs) remains poorly understood, partially due to the lack of genome-wide strategies to identify their targets. Here, we performed comprehensive microRNA profiling in hESCs and in purified neural and mesenchymal derivatives. Using a combination of AGO cross-linking and microRNA perturbation experiments, together with computational prediction, we identified the targets of the miR-302/367 cluster, the most abundant microRNAs in hESCs. Functional studies identified novel roles of miR-302/367 in maintaining pluripotency and regulating hESC differentiation. We show that in addition to its role in TGF-beta signaling, miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1. This study broadens our understanding of microRNA function in hESCs and is a valuable resource for future studies in this area.
LinkOut: [PMID: 22012620]
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CLIP-seq Support 1 for dataset SRR359787 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | hESCs (WA-09) / 4-thiouridine, RNase T1 |
Location of target site | ENST00000448108.2 | 3UTR | AUGUUAUGAUGAUGGGCGAAAUGUUCAACUGCUCUGAAGGGGCUGAAUGAAAAUGGCCUUUCUGAACAUCCAUUU |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 22012620 / SRX103431 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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94 hsa-miR-4753-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT064916 | ZBTB18 | zinc finger and BTB domain containing 18 | ![]() |
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2 | 2 | ||||||
MIRT161166 | SLC25A36 | solute carrier family 25 member 36 | ![]() |
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2 | 2 | ||||||
MIRT285542 | CDT1 | chromatin licensing and DNA replication factor 1 | ![]() |
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2 | 2 | ||||||
MIRT308266 | LRIG1 | leucine rich repeats and immunoglobulin like domains 1 | ![]() |
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2 | 2 | ||||||
MIRT311425 | LMNB1 | lamin B1 | ![]() |
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2 | 2 | ||||||
MIRT373989 | PEBP1 | phosphatidylethanolamine binding protein 1 | ![]() |
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2 | 4 | ||||||
MIRT383141 | CRY2 | cryptochrome circadian clock 2 | ![]() |
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2 | 2 | ||||||
MIRT405243 | ADIPOR2 | adiponectin receptor 2 | ![]() |
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2 | 2 | ||||||
MIRT441620 | ROCK1 | Rho associated coiled-coil containing protein kinase 1 | ![]() |
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2 | 6 | ||||||
MIRT441789 | SRPK1 | SRSF protein kinase 1 | ![]() |
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2 | 2 | ||||||
MIRT441804 | NOC3L | NOC3 like DNA replication regulator | ![]() |
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2 | 2 | ||||||
MIRT441832 | ALG14 | ALG14, UDP-N-acetylglucosaminyltransferase subunit | ![]() |
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2 | 2 | ||||||
MIRT442005 | NDUFV3 | NADH:ubiquinone oxidoreductase subunit V3 | ![]() |
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2 | 2 | ||||||
MIRT442411 | LIMD1 | LIM domains containing 1 | ![]() |
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2 | 2 | ||||||
MIRT442708 | UBE4B | ubiquitination factor E4B | ![]() |
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2 | 2 | ||||||
MIRT442714 | TNRC6B | trinucleotide repeat containing 6B | ![]() |
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2 | 2 | ||||||
MIRT442738 | SERINC5 | serine incorporator 5 | ![]() |
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2 | 2 | ||||||
MIRT442793 | CEP170 | centrosomal protein 170 | ![]() |
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2 | 2 | ||||||
MIRT442984 | ZNF736 | zinc finger protein 736 | ![]() |
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2 | 2 | ||||||
MIRT443055 | THRB | thyroid hormone receptor beta | ![]() |
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2 | 2 | ||||||
MIRT443288 | ZC3H12A | zinc finger CCCH-type containing 12A | ![]() |
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2 | 2 | ||||||
MIRT443325 | SLC35G1 | solute carrier family 35 member G1 | ![]() |
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2 | 2 | ||||||
MIRT443331 | OCRL | OCRL, inositol polyphosphate-5-phosphatase | ![]() |
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2 | 2 | ||||||
MIRT443593 | ZNF439 | zinc finger protein 439 | ![]() |
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2 | 4 | ||||||
MIRT443696 | KCNN3 | potassium calcium-activated channel subfamily N member 3 | ![]() |
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2 | 2 | ||||||
MIRT443748 | ELL2 | elongation factor for RNA polymerase II 2 | ![]() |
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2 | 2 | ||||||
MIRT443866 | HDLBP | high density lipoprotein binding protein | ![]() |
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2 | 2 | ||||||
MIRT461185 | LTBP2 | latent transforming growth factor beta binding protein 2 | ![]() |
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2 | 2 | ||||||
MIRT464074 | WAC | WW domain containing adaptor with coiled-coil | ![]() |
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2 | 2 | ||||||
MIRT468738 | SDC4 | syndecan 4 | ![]() |
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2 | 2 | ||||||
MIRT470540 | COASY | Coenzyme A synthase | ![]() |
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2 | 2 | ||||||
MIRT476458 | GBA2 | glucosylceramidase beta 2 | ![]() |
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2 | 2 | ||||||
MIRT479470 | CDK6 | cyclin dependent kinase 6 | ![]() |
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2 | 2 | ||||||
MIRT486351 | TACC2 | transforming acidic coiled-coil containing protein 2 | ![]() |
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2 | 8 | ||||||
MIRT495126 | CXorf67 | chromosome X open reading frame 67 | ![]() |
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2 | 2 | ||||||
MIRT495166 | CNGA2 | cyclic nucleotide gated channel alpha 2 | ![]() |
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2 | 4 | ||||||
MIRT495432 | ATG7 | autophagy related 7 | ![]() |
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2 | 2 | ||||||
MIRT495922 | FBXO41 | F-box protein 41 | ![]() |
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2 | 2 | ||||||
MIRT496572 | DGCR6L | DiGeorge syndrome critical region gene 6 like | ![]() |
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2 | 2 | ||||||
MIRT498277 | POFUT1 | protein O-fucosyltransferase 1 | ![]() |
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2 | 2 | ||||||
MIRT498431 | DDX39A | DExD-box helicase 39A | ![]() |
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2 | 2 | ||||||
MIRT530149 | HADHB | hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit | ![]() |
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2 | 2 | ||||||
MIRT530313 | TNFRSF10D | TNF receptor superfamily member 10d | ![]() |
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2 | 2 | ||||||
MIRT530880 | TRUB1 | TruB pseudouridine synthase family member 1 | ![]() |
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2 | 4 | ||||||
MIRT531177 | ZNF626 | zinc finger protein 626 | ![]() |
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2 | 2 | ||||||
MIRT533395 | TYRP1 | tyrosinase related protein 1 | ![]() |
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2 | 2 | ||||||
MIRT533709 | TMEM64 | transmembrane protein 64 | ![]() |
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2 | 2 | ||||||
MIRT533954 | TAF1D | TATA-box binding protein associated factor, RNA polymerase I subunit D | ![]() |
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2 | 2 | ||||||
MIRT535616 | NSD1 | nuclear receptor binding SET domain protein 1 | ![]() |
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2 | 2 | ||||||
MIRT539472 | ADARB2 | adenosine deaminase, RNA specific B2 (inactive) | ![]() |
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2 | 2 | ||||||
MIRT542878 | NR6A1 | nuclear receptor subfamily 6 group A member 1 | ![]() |
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2 | 2 | ||||||
MIRT559077 | C19orf47 | chromosome 19 open reading frame 47 | ![]() |
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2 | 2 | ||||||
MIRT559465 | ARPP19 | cAMP regulated phosphoprotein 19 | ![]() |
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2 | 2 | ||||||
MIRT561233 | ZNF772 | zinc finger protein 772 | ![]() |
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2 | 2 | ||||||
MIRT563477 | POLE3 | DNA polymerase epsilon 3, accessory subunit | ![]() |
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2 | 2 | ||||||
MIRT563995 | SLFN11 | schlafen family member 11 | ![]() |
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2 | 2 | ||||||
MIRT564222 | SDE2 | SDE2 telomere maintenance homolog | ![]() |
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2 | 2 | ||||||
MIRT566017 | RHOA | ras homolog family member A | ![]() |
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2 | 2 | ||||||
MIRT566028 | RFX1 | regulatory factor X1 | ![]() |
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2 | 2 | ||||||
MIRT566591 | NUFIP2 | NUFIP2, FMR1 interacting protein 2 | ![]() |
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2 | 2 | ||||||
MIRT567874 | CTDSP1 | CTD small phosphatase 1 | ![]() |
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2 | 2 | ||||||
MIRT568552 | AKT2 | AKT serine/threonine kinase 2 | ![]() |
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2 | 2 | ||||||
MIRT569357 | EFHC1 | EF-hand domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT569973 | DNAAF2 | dynein axonemal assembly factor 2 | ![]() |
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2 | 2 | ||||||
MIRT614423 | ZNF440 | zinc finger protein 440 | ![]() |
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2 | 2 | ||||||
MIRT628831 | SLC25A34 | solute carrier family 25 member 34 | ![]() |
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2 | 2 | ||||||
MIRT630142 | ZFYVE9 | zinc finger FYVE-type containing 9 | ![]() |
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2 | 2 | ||||||
MIRT634479 | PAFAH1B2 | platelet activating factor acetylhydrolase 1b catalytic subunit 2 | ![]() |
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2 | 2 | ||||||
MIRT634498 | OR7D2 | olfactory receptor family 7 subfamily D member 2 | ![]() |
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2 | 2 | ||||||
MIRT637394 | R3HDM2 | R3H domain containing 2 | ![]() |
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2 | 2 | ||||||
MIRT641840 | TCF7L2 | transcription factor 7 like 2 | ![]() |
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2 | 2 | ||||||
MIRT644397 | CDKL1 | cyclin dependent kinase like 1 | ![]() |
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2 | 2 | ||||||
MIRT644888 | C2orf50 | chromosome 2 open reading frame 50 | ![]() |
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2 | 2 | ||||||
MIRT647124 | ZNF446 | zinc finger protein 446 | ![]() |
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2 | 2 | ||||||
MIRT647420 | SSTR3 | somatostatin receptor 3 | ![]() |
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2 | 2 | ||||||
MIRT650297 | PYCARD | PYD and CARD domain containing | ![]() |
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2 | 2 | ||||||
MIRT655488 | PAK3 | p21 (RAC1) activated kinase 3 | ![]() |
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2 | 2 | ||||||
MIRT658185 | FBXO9 | F-box protein 9 | ![]() |
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2 | 2 | ||||||
MIRT660931 | ADAM19 | ADAM metallopeptidase domain 19 | ![]() |
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2 | 2 | ||||||
MIRT665317 | ZBTB3 | zinc finger and BTB domain containing 3 | ![]() |
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2 | 2 | ||||||
MIRT670350 | C1orf106 | chromosome 1 open reading frame 106 | ![]() |
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2 | 4 | ||||||
MIRT670823 | NICN1 | nicolin 1 | ![]() |
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2 | 2 | ||||||
MIRT671825 | TRPM6 | transient receptor potential cation channel subfamily M member 6 | ![]() |
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2 | 2 | ||||||
MIRT672732 | NETO2 | neuropilin and tolloid like 2 | ![]() |
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2 | 2 | ||||||
MIRT674841 | GLRX2 | glutaredoxin 2 | ![]() |
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2 | 2 | ||||||
MIRT675930 | CYP51A1 | cytochrome P450 family 51 subfamily A member 1 | ![]() |
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2 | 2 | ||||||
MIRT686786 | AZF1 | azoospermia factor 1 | ![]() |
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2 | 2 | ||||||
MIRT697723 | USP8 | ubiquitin specific peptidase 8 | ![]() |
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2 | 2 | ||||||
MIRT702357 | KLHL26 | kelch like family member 26 | ![]() |
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2 | 2 | ||||||
MIRT704361 | DBR1 | debranching RNA lariats 1 | ![]() |
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2 | 2 | ||||||
MIRT709242 | RANGAP1 | Ran GTPase activating protein 1 | ![]() |
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2 | 2 | ||||||
MIRT714433 | SNED1 | sushi, nidogen and EGF like domains 1 | ![]() |
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2 | 2 | ||||||
MIRT717000 | ARL6IP4 | ADP ribosylation factor like GTPase 6 interacting protein 4 | ![]() |
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2 | 2 | ||||||
MIRT720875 | ADCY5 | adenylate cyclase 5 | ![]() |
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2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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