>MAOB|NM_000898|3'UTR
   1 AGAGAGAGGGTGTCTGTAATCACACTCTCTTCTTACTGTATTTGGGATATGAGTTTGGGGAAAGAGTTGCAGTAAAGTTC
  81 CATGAAGACAAATAGTGTGGAGTGAGGCGGGGAGCATGAAGATAAATCCAACTCTGACTGTAAAATACATGGTATCTCTT
 161 TCTCCGTTGTGGCCCCTGCTTAGTGTCCCTTACCTGGCTTAGCGTTCTGTTTCACCAGTTTCCAAGTTTATTGCCCTCAA
 241 AATCTTTAGAATAGTTAAATTGGCTTGTTTAAGGTTCTTGCTGCCCCACAACACACCTTGCCCATGCACAAGGAATGAAT
 321 TTTTTCCTACCATTATGGCTTTGTGCTTGTTCTTCCTCTTACCTGTAATAGCCTCACCTTCCCTAGTTCTTTGCATTCGT
 401 CCTTAGAATACTGTATTGTTACAGCTGAAAGACAGTAAAGACCATTTAGTCCTCACCTTCTGTTTTAGAGTTGAGCAAAC
 481 TGAAGCCCACAGAGGTGGAACTTAATTACCTAAGAGCCACAATAAGCCACTGGTATCTGGGGGACTAGAACACAAATCCA
 561 ACGCTTTTCCCACCTCTTTGGATGTTTTCCCCAATTATCCTCCTTCACTCCCTGTCATAGTTACCGATGGTGTCCCGTTG
 641 TGTGGGTTTACTCTGTGCTAAGTTGTCTTACACTTCTCAAATGCTACTCAGTATATAGCCTTAAGTCTTACTGTTTTGTG
 721 CGGTGTGTCTCCAGCTGATTTTAACTTTTTTGATGGTAGAAATTTTATCTCTTCTTCCTTTTGTATCCTCCATTGTATCT
 801 TCATACAAAGGACAGTACACACTTGGGTAATTAAAAATAAAAGTTGATTGACCATAAAAAAAAAAAAAAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' ggagaUCAUUUAUACAGUCGUa 5' || :|: :|||| |:| Target 5' ---agAGAGAGGGTGTCTGTAa 3'	1 - 19	109.00	-7.10
2	miRNA 3' ggAGA--UCAUUUAUACAGUCGUa 5' ||| | || | | | |||:| Target 5' taTCTTCA-TACAAAGGACAGTAc 3'	796 - 818	105.00	-7.80
3	miRNA 3' ggAGAUCAUUUAUACA--GUCGUa 5' |:|:| :|| : || |||:| Target 5' agTTTGGGGAAAGAGTTGCAGTAa 3'	52 - 75	104.00	-5.90

Mutant ID	Mutant Position	Mutant Source
rs151333207	1	dbSNP
rs1211591459	2	dbSNP
rs1392405223	3	dbSNP
rs1484185976	7	dbSNP
rs1484230123	12	dbSNP
rs765126666	13	dbSNP
rs1231849165	20	dbSNP
rs761668830	21	dbSNP
rs777026159	34	dbSNP
rs762675192	40	dbSNP
rs1350261140	44	dbSNP
rs776631612	58	dbSNP
rs1189245304	59	dbSNP
rs1392269506	61	dbSNP
rs1426892391	82	dbSNP
rs770849796	87	dbSNP
rs901384329	89	dbSNP
rs1468070613	92	dbSNP
rs1335276760	102	dbSNP
rs1405938691	104	dbSNP
rs1040368323	108	dbSNP
rs942351287	109	dbSNP
rs878993126	122	dbSNP
rs1299096351	130	dbSNP
rs1260317669	132	dbSNP
rs1186408887	134	dbSNP
rs1301783461	135	dbSNP
rs895217777	146	dbSNP
rs1201498556	147	dbSNP
rs760469313	153	dbSNP
rs909570039	158	dbSNP
rs190704929	165	dbSNP
rs1048496085	166	dbSNP
rs1243915047	167	dbSNP
rs1474582284	189	dbSNP
rs929757988	203	dbSNP
rs7066677	204	dbSNP
rs539888650	207	dbSNP
rs1323099036	208	dbSNP
rs992714706	209	dbSNP
rs1263988686	213	dbSNP
rs1429208410	213	dbSNP
rs1225497431	214	dbSNP
rs17462	217	dbSNP
rs778519708	230	dbSNP
rs3027440	232	dbSNP
rs1381612786	238	dbSNP
rs1429641296	244	dbSNP
rs1293783905	256	dbSNP
rs1362515293	260	dbSNP
rs1369228245	266	dbSNP
rs746228550	270	dbSNP
rs1282322555	271	dbSNP
rs1329846893	275	dbSNP
rs1230137519	285	dbSNP
rs1450176801	294	dbSNP
rs1404974262	299	dbSNP
rs1286488833	300	dbSNP
rs186041127	303	dbSNP
rs1215105616	304	dbSNP
rs781100777	313	dbSNP
rs35285191	326	dbSNP
rs1490884456	338	dbSNP
rs956812646	339	dbSNP
rs911535883	350	dbSNP
rs1473235541	351	dbSNP
rs771023208	352	dbSNP
rs1406372994	365	dbSNP
rs1399639718	373	dbSNP
rs2072745	384	dbSNP
rs777921927	398	dbSNP
rs1039932076	399	dbSNP
rs1007575725	401	dbSNP
rs1050990	407	dbSNP
rs751962299	411	dbSNP
rs888101176	412	dbSNP
rs921660346	421	dbSNP
rs1370872131	422	dbSNP
rs1048083590	428	dbSNP
rs929725434	443	dbSNP
rs1349801475	444	dbSNP
rs1223303218	448	dbSNP
rs989287407	449	dbSNP
rs1422191751	452	dbSNP
rs1220512871	455	dbSNP
rs918296441	465	dbSNP
rs1030834347	479	dbSNP
rs1184366629	489	dbSNP
rs758341670	520	dbSNP
rs977977784	531	dbSNP
rs970644605	536	dbSNP
rs1205774003	547	dbSNP
rs1386543483	550	dbSNP
rs1422414413	551	dbSNP
rs1051000	562	dbSNP
rs1057295517	563	dbSNP
rs868015602	569	dbSNP
rs112372408	574	dbSNP
rs3027439	577	dbSNP
rs1297619558	582	dbSNP
rs980026875	589	dbSNP
rs1243560765	602	dbSNP
rs1385048817	608	dbSNP
rs968655924	617	dbSNP
rs915240396	620	dbSNP
rs1024923548	625	dbSNP
rs3027438	628	dbSNP
rs182018652	636	dbSNP
rs1393796346	637	dbSNP
rs895186561	640	dbSNP
rs1027476031	642	dbSNP
rs1031063225	645	dbSNP
rs1442269692	646	dbSNP
rs1462529797	647	dbSNP
rs1390414396	665	dbSNP
rs1803940	669	dbSNP
rs190636944	672	dbSNP
rs998694365	677	dbSNP
rs965555030	678	dbSNP
rs1476150568	690	dbSNP
rs1166002784	691	dbSNP
rs1018885791	693	dbSNP
rs1395480512	701	dbSNP
rs1415015000	702	dbSNP
rs1051003	705	dbSNP
rs1162199829	710	dbSNP
rs1403754301	714	dbSNP
rs995612100	721	dbSNP
rs1007136612	722	dbSNP
rs1366284138	724	dbSNP
rs888738683	734	dbSNP
rs1268836865	736	dbSNP
rs766225528	743	dbSNP
rs1417574180	749	dbSNP
rs760546092	755	dbSNP
rs1220137935	757	dbSNP
rs772995351	758	dbSNP
rs1460324817	764	dbSNP
rs1196513612	769	dbSNP
rs184515383	770	dbSNP
rs896842366	771	dbSNP
rs1056861464	781	dbSNP
rs1276733435	792	dbSNP
rs180943482	797	dbSNP
rs149564601	801	dbSNP
rs1372106596	805	dbSNP
rs1478248928	811	dbSNP
rs905683709	814	dbSNP
rs868171694	815	dbSNP
rs1469226455	818	dbSNP
rs1049991522	821	dbSNP
rs1385731965	825	dbSNP
rs947204674	825	dbSNP
rs1384175290	826	dbSNP
rs914455001	827	dbSNP
rs768159471	835	dbSNP
rs139274476	844	dbSNP
rs1285509662	848	dbSNP

- Genes & development, 2011

MicroRNAs are important regulators in many cellular processes, including stem cell self-renewal. Recent studies demonstrated their function as pluripotency factors with the capacity for somatic cell reprogramming. However, their role in human embryonic stem (ES) cells (hESCs) remains poorly understood, partially due to the lack of genome-wide strategies to identify their targets. Here, we performed comprehensive microRNA profiling in hESCs and in purified neural and mesenchymal derivatives. Using a combination of AGO cross-linking and microRNA perturbation experiments, together with computational prediction, we identified the targets of the miR-302/367 cluster, the most abundant microRNAs in hESCs. Functional studies identified novel roles of miR-302/367 in maintaining pluripotency and regulating hESC differentiation. We show that in addition to its role in TGF-beta signaling, miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1. This study broadens our understanding of microRNA function in hESCs and is a valuable resource for future studies in this area.