miRTarBase - #MIRT543140


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pre-miRNA Information
pre-miRNA hsa-mir-378f   
Genomic Coordinates chr1: 23929070 - 23929147
Description Homo sapiens miR-378f stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-378f
Sequence 52| ACUGGACUUGGAGCCAGAAG |71
Evidence Experimental
Experiments Illumina
Editing Events in miRNAs
Modification Type Position on miR Chromosome DNA Strand Genomic Position (hg38) List of PMIDs Variant details
A-to-I 18 1 + 23929138 29233923 MiREDiBase
A-to-I 19 1 + 23929139 29233923 MiREDiBase
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol AKT1   
Synonyms AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Description AKT serine/threonine kinase 1
Transcript NM_001014431   
Other Transcripts NM_001014432 , NM_005163   
Expression
Putative miRNA Targets on AKT1
3'UTR of AKT1
(miRNA target sites are highlighted)		 
>AKT1|NM_001014431|3'UTR
   1 GGCGGCGGTGGACTGCGCTGGACGATAGCTTGGAGGGATGGAGAGGCGGCCTCGTGCCATGATCTGTATTTAATGGTTTT
  81 TATTTCTCGGGTGCATTTGAGAGAAGCCACGCTGTCCTCTCGAGCCCAGATGGAAAGACGTTTTTGTGCTGTGGGCAGCA
 161 CCCTCCCCCGCAGCGGGGTAGGGAAGAAAACTATCCTGCGGGTTTTAATTTATTTCATCCAGTTTGTTCTCCGGGTGTGG
 241 CCTCAGCCCTCAGAACAATCCGATTCACGTAGGGAAATGTTAAGGACTTCTGCAGCTATGCGCAATGTGGCATTGGGGGG
 321 CCGGGCAGGTCCTGCCCATGTGTCCCCTCACTCTGTCAGCCAGCCGCCCTGGGCTGTCTGTCACCAGCTATCTGTCATCT
 401 CTCTGGGGCCCTGGGCCTCAGTTCAACCTGGTGGCACCAGATGCAACCTCACTATGGTATGCTGGCCAGCACCCTCTCCT
 481 GGGGGTGGCAGGCACACAGCAGCCCCCCAGCACTAAGGCCGTGTCTCTGAGGACGTCATCGGAGGCTGGGCCCCTGGGAT
 561 GGGACCAGGGATGGGGGATGGGCCAGGGTTTACCCAGTGGGACAGAGGAGCAAGGTTTAAATTTGTTATTGTGTATTATG
 641 TTGTTCAAATGCATTTTGGGGGTTTTTAATCTTTGTGACAGGAAAGCCCTCCCCCTTCCCCTTCTGTGTCACAGTTCTTG
 721 GTGACTGTCCCACCGGGAGCCTCCCCCTCAGATGATCTCTCCACGGTAGCACTTGACCTTTTCGACGCTTAACCTTTCCG
 801 CTGTCGCCCCAGGCCCTCCCTGACTCCCTGTGGGGGTGGCCATCCCTGGGCCCCTCCACGCCTCCTGGCCAGACGCTGCC
 881 GCTGCCGCTGCACCACGGCGTTTTTTTACAACATTCAACTTTAGTATTTTTACTATTATAATATAATATGGAACCTTCCC
 961 TCCAAATTCTTCAATAAAAGTTGCTTTTCAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gaaGAC-CGAG-GUUCAGGUCa 5'
             |||  ||| |:|| |||| 
Target 5' acgCTGTCCTCTCGAGCCCAGa 3'
 109 - 130 125.00 -16.40
2 
miRNA  3' gaaGACCGAGGUUCAGGUca 5'
             ||||  |: |||:||  
Target 5' gccCTGGGCCTCAGTTCAac 3'
 408 - 427 121.00 -12.30
3 
miRNA  3' gaAGACCGAGGUUCAGGuca 5'
            ||||  ||  |||:|   
Target 5' ctTCTGTGTCACAGTTCttg 3'
 701 - 720 102.00 -11.42
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30158185 1 COSMIC
COSN30102583 6 COSMIC
COSN26965712 12 COSMIC
COSN30133970 16 COSMIC
COSN30591041 19 COSMIC
COSN13573317 23 COSMIC
COSN26440683 32 COSMIC
COSN26965709 46 COSMIC
COSN30190149 49 COSMIC
COSN31611744 129 COSMIC
COSN1164859 141 COSMIC
COSN1638710 647 COSMIC
COSN30580631 663 COSMIC
COSN30605709 836 COSMIC
COSN30753976 859 COSMIC
COSN26225335 900 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs780299611 3 dbSNP
rs756226133 4 dbSNP
rs200508405 6 dbSNP
rs192237287 7 dbSNP
rs111793056 8 dbSNP
rs752606488 10 dbSNP
rs1461248384 11 dbSNP
rs941228566 14 dbSNP
rs377339529 16 dbSNP
rs373329736 17 dbSNP
rs1436788573 18 dbSNP
rs1395823104 21 dbSNP
rs375585585 23 dbSNP
rs1355946499 24 dbSNP
rs370987171 24 dbSNP
rs1003565559 27 dbSNP
rs558891046 35 dbSNP
rs539258432 37 dbSNP
rs761112590 39 dbSNP
rs1231525232 41 dbSNP
rs368920620 47 dbSNP
rs573353277 48 dbSNP
rs11555430 49 dbSNP
rs1231382981 51 dbSNP
rs1045350619 53 dbSNP
rs1029593907 54 dbSNP
rs977181153 73 dbSNP
rs553657544 86 dbSNP
rs1239132286 88 dbSNP
rs1232438206 91 dbSNP
rs17846821 110 dbSNP
rs1190755940 111 dbSNP
rs17846826 121 dbSNP
rs1286175037 122 dbSNP
rs1254964776 124 dbSNP
rs1407865771 126 dbSNP
rs904653860 127 dbSNP
rs1222390134 130 dbSNP
rs1322315643 135 dbSNP
rs548181379 136 dbSNP
rs1436017209 137 dbSNP
rs923714710 138 dbSNP
rs537945247 139 dbSNP
rs1400472051 140 dbSNP
rs1020473366 142 dbSNP
rs1010464241 157 dbSNP
rs1359595334 158 dbSNP
rs933014347 165 dbSNP
rs896091178 166 dbSNP
rs921830745 169 dbSNP
rs373002859 170 dbSNP
rs939045923 174 dbSNP
rs1194439197 175 dbSNP
rs1244262498 186 dbSNP
rs904934355 191 dbSNP
rs1185623460 195 dbSNP
rs1045172220 197 dbSNP
rs909012059 199 dbSNP
rs983287538 212 dbSNP
rs941120153 215 dbSNP
rs1025741767 218 dbSNP
rs112281303 230 dbSNP
rs1420300357 232 dbSNP
rs982509372 233 dbSNP
rs1343263655 236 dbSNP
rs909688005 239 dbSNP
rs1429725493 241 dbSNP
rs1275175111 242 dbSNP
rs61761249 249 dbSNP
rs1294837237 259 dbSNP
rs1436548924 261 dbSNP
rs1206301361 266 dbSNP
rs34558581 268 dbSNP
rs1012484456 270 dbSNP
rs1253958787 273 dbSNP
rs532385857 275 dbSNP
rs1191354232 278 dbSNP
rs894066817 295 dbSNP
rs1422002741 296 dbSNP
rs1470176544 299 dbSNP
rs41307094 301 dbSNP
rs976770620 302 dbSNP
rs902230787 304 dbSNP
rs1396324558 305 dbSNP
rs1443472130 310 dbSNP
rs770697761 313 dbSNP
rs964432061 322 dbSNP
rs1130245 323 dbSNP
rs1038727422 328 dbSNP
rs137981458 329 dbSNP
rs1223652989 336 dbSNP
rs560947797 338 dbSNP
rs1274269933 339 dbSNP
rs909147158 343 dbSNP
rs544158185 344 dbSNP
rs1211490812 347 dbSNP
rs1251952012 349 dbSNP
rs1438003719 350 dbSNP
rs983253841 355 dbSNP
rs575655519 358 dbSNP
rs968803206 361 dbSNP
rs929182608 365 dbSNP
rs575222918 366 dbSNP
rs34865723 368 dbSNP
rs1163103103 376 dbSNP
rs113613978 379 dbSNP
rs756938164 383 dbSNP
rs1233277990 385 dbSNP
rs1330982592 390 dbSNP
rs1334051808 390 dbSNP
rs35416681 391 dbSNP
rs1302917602 394 dbSNP
rs960266704 394 dbSNP
rs1294418810 395 dbSNP
rs1337455926 397 dbSNP
rs573418247 400 dbSNP
rs553256558 401 dbSNP
rs904820537 405 dbSNP
rs1402397226 406 dbSNP
rs958261281 408 dbSNP
rs1197117739 416 dbSNP
rs1032571139 431 dbSNP
rs1044746909 432 dbSNP
rs1005232632 433 dbSNP
rs765101207 436 dbSNP
rs539274348 437 dbSNP
rs1046867370 441 dbSNP
rs754575126 444 dbSNP
rs1186987382 460 dbSNP
rs1373366051 476 dbSNP
rs1177093543 479 dbSNP
rs1428090408 483 dbSNP
rs1169857695 487 dbSNP
rs1394481557 492 dbSNP
rs574337857 496 dbSNP
rs554616284 503 dbSNP
rs1328722474 508 dbSNP
rs1041321824 509 dbSNP
rs1478796473 510 dbSNP
rs997241235 519 dbSNP
rs537882189 520 dbSNP
rs911185309 521 dbSNP
rs568759365 522 dbSNP
rs1229232142 529 dbSNP
rs1209947076 532 dbSNP
rs570600044 534 dbSNP
rs968773919 535 dbSNP
rs913350301 540 dbSNP
rs1211020994 541 dbSNP
rs1038695378 543 dbSNP
rs1804268 545 dbSNP
rs1257494767 546 dbSNP
rs988973319 548 dbSNP
rs1210069550 549 dbSNP
rs1130295 554 dbSNP
rs1210398718 558 dbSNP
rs960235921 560 dbSNP
rs1430058361 565 dbSNP
rs1035870119 566 dbSNP
rs1371583772 576 dbSNP
rs974939803 583 dbSNP
rs1174350065 589 dbSNP
rs751165631 592 dbSNP
rs1433481521 606 dbSNP
rs1002161867 608 dbSNP
rs780314185 614 dbSNP
rs1384245699 624 dbSNP
rs1047698588 629 dbSNP
rs970351428 631 dbSNP
rs1404826037 639 dbSNP
rs928519104 648 dbSNP
rs1023703361 651 dbSNP
rs1318675066 668 dbSNP
rs758739510 669 dbSNP
rs566232803 676 dbSNP
rs1370964922 677 dbSNP
rs1314465367 681 dbSNP
rs1416879397 685 dbSNP
rs1376253212 687 dbSNP
rs1173097222 691 dbSNP
rs916684347 692 dbSNP
rs990753249 693 dbSNP
rs866890023 696 dbSNP
rs1194502878 700 dbSNP
rs958061670 701 dbSNP
rs1130443 704 dbSNP
rs1365590744 705 dbSNP
rs1397034649 714 dbSNP
rs1452636920 721 dbSNP
rs1160556377 727 dbSNP
rs1418973363 729 dbSNP
rs1248918605 732 dbSNP
rs888187782 734 dbSNP
rs552278695 735 dbSNP
rs1256282558 737 dbSNP
rs1208265801 741 dbSNP
rs1283540443 743 dbSNP
rs1486601827 746 dbSNP
rs1260774868 754 dbSNP
rs1238307282 755 dbSNP
rs1385762648 757 dbSNP
rs978353159 757 dbSNP
rs1315006935 758 dbSNP
rs112114610 764 dbSNP
rs566677791 765 dbSNP
rs1214609516 766 dbSNP
rs1255091552 767 dbSNP
rs1356354848 767 dbSNP
rs1467279979 773 dbSNP
rs1312560259 775 dbSNP
rs1464625826 776 dbSNP
rs559789852 778 dbSNP
rs1421404174 780 dbSNP
rs964463995 782 dbSNP
rs1017393242 783 dbSNP
rs1289495993 784 dbSNP
rs1453697124 785 dbSNP
rs762049014 786 dbSNP
rs186886987 787 dbSNP
rs3840005 787 dbSNP
rs55707948 787 dbSNP
rs1167554548 788 dbSNP
rs1298252146 790 dbSNP
rs769089409 795 dbSNP
rs749839506 799 dbSNP
rs1185017445 800 dbSNP
rs1389663606 801 dbSNP
rs1429909276 803 dbSNP
rs753950090 805 dbSNP
rs1355899092 806 dbSNP
rs764172888 808 dbSNP
rs1270987258 813 dbSNP
rs3803305 818 dbSNP
rs567261290 820 dbSNP
rs1232012323 826 dbSNP
rs1342779367 827 dbSNP
rs1176026587 832 dbSNP
rs1313959811 837 dbSNP
rs1251765008 838 dbSNP
rs907119826 840 dbSNP
rs911167671 844 dbSNP
rs17846828 853 dbSNP
rs58565216 854 dbSNP
rs1428273305 858 dbSNP
rs913318973 859 dbSNP
rs17846829 860 dbSNP
rs936673159 863 dbSNP
rs925321957 869 dbSNP
rs183360200 874 dbSNP
rs1456386277 877 dbSNP
rs1416481602 878 dbSNP
rs17102385 880 dbSNP
rs923531097 881 dbSNP
rs528550066 886 dbSNP
rs140320743 887 dbSNP
rs773176237 889 dbSNP
rs190605178 891 dbSNP
rs1017360624 892 dbSNP
rs1186786985 892 dbSNP
rs964897944 892 dbSNP
rs983748482 895 dbSNP
rs11555429 896 dbSNP
rs1025273425 897 dbSNP
rs993850076 899 dbSNP
rs1025949075 900 dbSNP
rs1457887928 902 dbSNP
rs1251909646 907 dbSNP
rs1207537639 908 dbSNP
rs1358402472 909 dbSNP
rs1431240310 909 dbSNP
rs1359136895 914 dbSNP
rs993590409 921 dbSNP
rs907090999 938 dbSNP
rs769719936 940 dbSNP
rs1297040945 942 dbSNP
rs1019848889 944 dbSNP
rs1237203739 950 dbSNP
rs1348665249 950 dbSNP
rs1238915903 962 dbSNP
rs1442311422 979 dbSNP
rs1216988908 980 dbSNP
rs1012735861 984 dbSNP
rs894353052 987 dbSNP
rs1311848591 991 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 207.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714645. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' gaagACCGAGGU-UCAGGUCa 5'
              | |:||:| ||||||| 
Target 5' --gaUAGUUCUAUAGUCCAGa 3'
 		1 - 19
Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
CLIP-seq Support 1 for dataset GSM714645
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repB
Location of target site ENST00000554581.1 | 3UTR | GAUAGUUCUAUAGUCCAGA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
27 hsa-miR-378f Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT463100 ZNF609 zinc finger protein 609 2 2
MIRT469371 REST RE1 silencing transcription factor 2 6
MIRT495441 KCNJ6 potassium voltage-gated channel subfamily J member 6 2 2
MIRT513606 VPS37B VPS37B, ESCRT-I subunit 2 2
MIRT520414 TXNL1 thioredoxin like 1 2 4
MIRT525111 PRKD2 protein kinase D2 2 2
MIRT525979 SPA17 sperm autoantigenic protein 17 2 2
MIRT527002 ARL8B ADP ribosylation factor like GTPase 8B 2 2
MIRT529215 CYP20A1 cytochrome P450 family 20 subfamily A member 1 2 2
MIRT529835 ARGFX arginine-fifty homeobox 2 2
MIRT532300 HAUS3 HAUS augmin like complex subunit 3 2 2
MIRT537577 ESYT2 extended synaptotagmin 2 2 2
MIRT543140 AKT1 AKT serine/threonine kinase 1 2 2
MIRT554924 RAP1B RAP1B, member of RAS oncogene family 2 2
MIRT564970 WTAP WT1 associated protein 2 2
MIRT570115 CACNG8 calcium voltage-gated channel auxiliary subunit gamma 8 2 2
MIRT652734 TGFB2 transforming growth factor beta 2 2 2
MIRT655948 NDST1 N-deacetylase and N-sulfotransferase 1 2 2
MIRT655997 MYRF myelin regulatory factor 2 2
MIRT684875 P4HB prolyl 4-hydroxylase subunit beta 2 2
MIRT692969 LGSN lengsin, lens protein with glutamine synthetase domain 2 2
MIRT693737 ACACA acetyl-CoA carboxylase alpha 2 2
MIRT710991 PPARGC1B PPARG coactivator 1 beta 2 2
MIRT712247 RCC2 regulator of chromosome condensation 2 2 2
MIRT718602 DCTPP1 dCTP pyrophosphatase 1 2 2
MIRT723499 WDR33 WD repeat domain 33 2 2
MIRT725448 HIPK3 homeodomain interacting protein kinase 3 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-378f Gemcitabine 60750 NSC613327 approved resistant High Pancreatic Cancer cell line (PANC-1)
hsa-miR-378f Fluorouracil 3385 NSC19893 approved resistant High Pancreatic Cancer cell line (PANC-1)
hsa-miR-378f Doxorubicin 31703 NSC123127 approved sensitive High Triple-Negative Breast Cancer cell line (MDA-MB-231, MDA-MB-468)
hsa-miR-378f Oxaliplatin 6857599 NSC266046 approved sensitive High Colorectal Cancer cell line (SW480, HCT-116)
hsa-miR-378f Erlotinib 176870 NSC718781 approved resistant High Head And Neck Squamous Cell Carcinoma cell line (HN6)
hsa-mir-378f Androstenedione+Anastrozole sensitive cell line (MCF-7)
hsa-miR-378f Osimertinib 71496458 NSC779217 approved sensitive cell line (PC9)
hsa-miR-378f Doxorubicin 31703 NSC123127 approved sensitive cell line (BAS)
hsa-miR-378f Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)
hsa-miR-378f Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-378f Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR4)
hsa-miR-378f Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR3)

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