pre-miRNA Information
pre-miRNA hsa-mir-758   
Genomic Coordinates chr14: 101026020 - 101026107
Synonyms MIRN758, hsa-mir-758, MIR758
Description Homo sapiens miR-758 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-758-5p
Sequence 15| GAUGGUUGACCAGAGAGCACAC |36
Evidence Experimental
Experiments SOLiD
Editing Events in miRNAs
Modification Type Position on miR Chromosome DNA Strand Genomic Position (hg38) List of PMIDs Variant details
A-to-I 16 14 + 101026049 29233923 MiREDiBase
A-to-I 21 14 + 101026054 29233923 MiREDiBase
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs372417522 1 dbSNP
rs748092616 2 dbSNP
rs1270901332 4 dbSNP
rs1364206491 5 dbSNP
rs1483583954 8 dbSNP
rs770931807 10 dbSNP
rs776760808 11 dbSNP
rs1360350664 12 dbSNP
rs759742151 13 dbSNP
rs1002938972 15 dbSNP
rs770107042 17 dbSNP
rs369242818 20 dbSNP
rs1217090272 21 dbSNP
rs763341052 22 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol PIGW   
Synonyms Gwt1, HPMRS5
Description phosphatidylinositol glycan anchor biosynthesis class W
Transcript NM_178517   
Expression
Putative miRNA Targets on PIGW
3'UTR of PIGW
(miRNA target sites are highlighted)
>PIGW|NM_178517|3'UTR
   1 TCAGCAGGAGTAGGATATATAAGTATTTGGGCAATATTTAATGAGGAATATTAATTGTAAAGAAATTGTGCTTTTGGCAA
  81 CAGTGTTAACCATATTTTATTATAAAATAGTTTCAGTCATCTAAACAGCAGTGATGCTTAATTATTTTTTTTTTTGAGAC
 161 AGAGTCTTGCTCTGTTACCCAGGCTGGAGTGTAGTGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCCGGGTTCAAGC
 241 AATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGCACTGTAGGCATGTACCACCATGCCCAGCTAATTTTTATATTTTTAGT
 321 AGAGATGGGGTTTTGCCATATTGGCCAGGCTGGTCTCAAACTCCTGAACTCAAGTGATAACACCCACCTTGGCCTCCCAA
 401 AGTGCTGGGATTACAGGTGTGAGCCACCACACTGGGCCAATGCTTAATATTTTAATGTATCTCAACAATAAAACCAAGAA
 481 GAAACAAAGCCTTTTGACTTGTTAGAATGTATTAAGTAGTATTTTAAAGAAACTTTATAGTTGTGACATTGAAAGACTGT
 561 TGGGGTGGGGGGAGGAAAATTTTTACTTTCCATCTTAATGTAACCTTATGCTATTCTGTATTTTTACTGTATATTGCTTT
 641 TACAATAAATATAAAATGAAATGTTTATGTTGACATTTCAGTGTGAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' caCACGAGAGAC-------CAGUUGGUAg 5'
            |||||:|: |       ||:|||||| 
Target 5' ttGTGCTTTTGGCAACAGTGTTAACCATa 3'
66 - 94 148.00 -17.10
2
miRNA  3' cacaCGAGAGACCAGUUGGuag 5'
              |||| |||  |||||   
Target 5' ctcaGCTCACTG--CAACCtcc 3'
204 - 223 122.00 -16.40
3
miRNA  3' caCA-CGA--GAGAC-------CA-GUUGGUAg 5'
            || |||  | |||       || | ||||| 
Target 5' gaGTAGCTGGCACTGTAGGCATGTACCACCATg 3'
263 - 295 114.00 -10.10
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1203955065 2 dbSNP
rs761430333 2 dbSNP
rs540012861 3 dbSNP
rs560069434 4 dbSNP
rs764762547 6 dbSNP
rs763872595 9 dbSNP
rs1249705379 12 dbSNP
rs1308601291 12 dbSNP
rs1292440182 13 dbSNP
rs1417265709 14 dbSNP
rs751510787 14 dbSNP
rs1315783774 15 dbSNP
rs375449647 15 dbSNP
rs750274624 16 dbSNP
rs1160492884 18 dbSNP
rs1481114982 19 dbSNP
rs1010976724 20 dbSNP
rs1381009169 20 dbSNP
rs1296246931 21 dbSNP
rs761022098 24 dbSNP
rs755776798 26 dbSNP
rs1446797529 42 dbSNP
rs1164548915 47 dbSNP
rs927876692 67 dbSNP
rs1172507301 68 dbSNP
rs1442670135 70 dbSNP
rs1022227605 72 dbSNP
rs1047317776 95 dbSNP
rs1446099012 129 dbSNP
rs1231871968 135 dbSNP
rs1194868246 137 dbSNP
rs1272644605 144 dbSNP
rs1481195498 144 dbSNP
rs1315845744 145 dbSNP
rs573322152 145 dbSNP
rs886974752 145 dbSNP
rs981394047 146 dbSNP
rs545163901 148 dbSNP
rs181960075 155 dbSNP
rs1306631890 157 dbSNP
rs1346608117 161 dbSNP
rs1373840785 175 dbSNP
rs1309582821 178 dbSNP
rs1422696072 181 dbSNP
rs1364827350 183 dbSNP
rs187132199 185 dbSNP
rs996188576 192 dbSNP
rs1257772413 203 dbSNP
rs1361541271 219 dbSNP
rs1029034584 220 dbSNP
rs867763935 221 dbSNP
rs753976547 231 dbSNP
rs1251848888 232 dbSNP
rs1433177736 246 dbSNP
rs1293229740 248 dbSNP
rs1196764470 250 dbSNP
rs1225353996 263 dbSNP
rs1009178261 266 dbSNP
rs1379459163 266 dbSNP
rs1480857067 267 dbSNP
rs1441146565 279 dbSNP
rs1260189002 281 dbSNP
rs551304302 283 dbSNP
rs1318912614 289 dbSNP
rs1398427019 293 dbSNP
rs956910065 296 dbSNP
rs1402804717 297 dbSNP
rs989149023 307 dbSNP
rs1463848973 321 dbSNP
rs914998112 322 dbSNP
rs1382186863 323 dbSNP
rs969143561 327 dbSNP
rs1299727442 331 dbSNP
rs982962893 337 dbSNP
rs144931880 339 dbSNP
rs1386149448 343 dbSNP
rs76722003 360 dbSNP
rs1320953748 364 dbSNP
rs939252110 372 dbSNP
rs757363137 375 dbSNP
rs908348816 376 dbSNP
rs941070459 378 dbSNP
rs1037756467 394 dbSNP
rs138661637 405 dbSNP
rs996302736 415 dbSNP
rs547242688 425 dbSNP
rs748766158 429 dbSNP
rs1230060334 431 dbSNP
rs1252254100 441 dbSNP
rs932343361 461 dbSNP
rs1208601758 469 dbSNP
rs1269502912 470 dbSNP
rs566934429 471 dbSNP
rs1317034246 486 dbSNP
rs891044864 490 dbSNP
rs1390189310 491 dbSNP
rs1468938499 492 dbSNP
rs796125391 492 dbSNP
rs1011020883 504 dbSNP
rs1421739005 511 dbSNP
rs1052946072 515 dbSNP
rs1022457955 517 dbSNP
rs1194517113 521 dbSNP
rs969091163 525 dbSNP
rs1241327103 534 dbSNP
rs980518203 537 dbSNP
rs892997969 541 dbSNP
rs1478963861 558 dbSNP
rs765876068 558 dbSNP
rs1010914836 562 dbSNP
rs76203149 563 dbSNP
rs1337372415 566 dbSNP
rs1269590885 567 dbSNP
rs1228038217 569 dbSNP
rs905160600 570 dbSNP
rs1366790692 572 dbSNP
rs1312233120 575 dbSNP
rs758807540 576 dbSNP
rs960422214 587 dbSNP
rs1035464669 591 dbSNP
rs960974120 609 dbSNP
rs983461790 610 dbSNP
rs551980930 615 dbSNP
rs568836225 619 dbSNP
rs1297111905 621 dbSNP
rs973784158 634 dbSNP
rs932043143 635 dbSNP
rs781349605 651 dbSNP
rs1435915770 653 dbSNP
rs1050549121 667 dbSNP
rs537416285 668 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 284098.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1 "PAR-CLIP data was present in GSM714645. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' cacacgagagaccAGUUGGUAg 5'
                       |:|||||| 
Target 5' -------------UUAACCAUa 3'
1 - 9
Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
CLIP-seq Support 1 for dataset GSM714644
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repA
Location of target site ENST00000328396.2 | 3UTR | UUAACCAUAUUUUAUUAUAAAAUAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM714645
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repB
Location of target site ENST00000328396.2 | 3UTR | UUAACCAUAUUUUAUUAUAAAAUAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
62 hsa-miR-758-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT085323 MORC3 MORC family CW-type zinc finger 3 2 2
MIRT089441 STAMBP STAM binding protein 2 2
MIRT089456 TET3 tet methylcytosine dioxygenase 3 2 2
MIRT111856 CCND1 cyclin D1 2 2
MIRT184933 ZNF268 zinc finger protein 268 2 2
MIRT215288 CREBRF CREB3 regulatory factor 2 2
MIRT237300 LPP LIM domain containing preferred translocation partner in lipoma 2 2
MIRT238446 MYO10 myosin X 2 4
MIRT273827 RPL41 ribosomal protein L41 2 2
MIRT282703 HOOK1 hook microtubule tethering protein 1 2 2
MIRT347970 ZNF850 zinc finger protein 850 2 2
MIRT371076 KLF3 Kruppel like factor 3 2 2
MIRT464339 USP6NL USP6 N-terminal like 2 2
MIRT470034 PTP4A1 protein tyrosine phosphatase type IVA, member 1 2 2
MIRT477506 ELL2 elongation factor for RNA polymerase II 2 2 2
MIRT482886 CACNA2D3 calcium voltage-gated channel auxiliary subunit alpha2delta 3 2 2
MIRT492606 POLR3E RNA polymerase III subunit E 2 2
MIRT502294 GNG12 G protein subunit gamma 12 2 6
MIRT507600 DCTN4 dynactin subunit 4 2 4
MIRT510728 SON SON DNA binding protein 2 6
MIRT514065 KCNJ6 potassium voltage-gated channel subfamily J member 6 2 8
MIRT519718 ZNF512B zinc finger protein 512B 2 4
MIRT520890 STRN striatin 2 2
MIRT521760 PPIL1 peptidylprolyl isomerase like 1 2 6
MIRT526874 ERCC8 ERCC excision repair 8, CSA ubiquitin ligase complex subunit 2 2
MIRT530232 WSB2 WD repeat and SOCS box containing 2 2 2
MIRT532003 ACTR2 ARP2 actin related protein 2 homolog 2 2
MIRT533371 UBE2D4 ubiquitin conjugating enzyme E2 D4 (putative) 2 4
MIRT547106 PIGW phosphatidylinositol glycan anchor biosynthesis class W 2 2
MIRT548189 FOXA1 forkhead box A1 2 2
MIRT552935 VKORC1L1 vitamin K epoxide reductase complex subunit 1 like 1 2 2
MIRT560085 ZNF195 zinc finger protein 195 2 2
MIRT561726 PPP2CA protein phosphatase 2 catalytic subunit alpha 2 2
MIRT562713 ZNF415 zinc finger protein 415 2 2
MIRT562761 ZNF846 zinc finger protein 846 2 2
MIRT564159 ZNF117 zinc finger protein 117 2 2
MIRT565673 SETD5 SET domain containing 5 2 2
MIRT565718 SESN3 sestrin 3 2 2
MIRT566026 RFX1 regulatory factor X1 2 2
MIRT569048 ZNF655 zinc finger protein 655 2 2
MIRT570367 UBE2V1 ubiquitin conjugating enzyme E2 V1 2 2
MIRT570410 TMEM189-UBE2V1 TMEM189-UBE2V1 readthrough 2 2
MIRT570443 TMEM189 transmembrane protein 189 2 2
MIRT571738 RNF11 ring finger protein 11 2 2
MIRT575042 Tpgs2 tubulin polyglutamylase complex subunit 2 2 4
MIRT614330 ZDHHC22 zinc finger DHHC-type containing 22 2 2
MIRT617629 RAB3IP RAB3A interacting protein 2 2
MIRT621667 UBE4B ubiquitination factor E4B 2 2
MIRT639906 SRGAP2 SLIT-ROBO Rho GTPase activating protein 2 2 2
MIRT651436 XRCC5 X-ray repair cross complementing 5 2 2
MIRT683853 ZNF208 zinc finger protein 208 2 2
MIRT684841 TPGS2 tubulin polyglutamylase complex subunit 2 2 5
MIRT689347 ZNF83 zinc finger protein 83 2 2
MIRT692492 SPIN4 spindlin family member 4 2 2
MIRT695711 OLA1 Obg like ATPase 1 2 2
MIRT698219 TMEM248 transmembrane protein 248 2 2
MIRT711560 FAM20B FAM20B, glycosaminoglycan xylosylkinase 2 2
MIRT712867 TMEM67 transmembrane protein 67 2 2
MIRT722956 TSPAN1 tetraspanin 1 2 2
MIRT723622 SOBP sine oculis binding protein homolog 2 2
MIRT724176 ABCF2 ATP binding cassette subfamily F member 2 2 2
MIRT755363 LMBR1 limb development membrane protein 1 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-758 Hydroxycamptothecin (HCPT) NULL 97226 Microarray human Tenon's fibroblasts (HTFs) 24681041 2014 down-regulated
miR-758 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 up-regulated
miR-758 5-Fluorouracil approved 3385 Quantitative real-time PCR human squamous cell carcinoma cell line KYSE410 21743970 2011 up-regulated
miR-758 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-758 Fluorouracil 3385 NSC19893 approved resistant cell line (KYSE)
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (HCC827)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Paclitaxel 36314 NSC125973 approved sensitive cell line (BAS)
hsa-miR-758-5p Doxorubicin 31703 NSC123127 approved sensitive cell line (BAS)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-758-5p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-758-5p Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)

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