pre-miRNA Information
pre-miRNA hsa-mir-921   
Genomic Coordinates chr1: 166154743 - 166154798
Synonyms MIRN921, hsa-mir-921, MIR921
Description Homo sapiens miR-921 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-921
Sequence 2| CUAGUGAGGGACAGAACCAGGAUUC |26
Evidence Experimental
Experiments Cloned
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
COSN31549221 11 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1464259216 3 dbSNP
rs768428855 8 dbSNP
rs746970999 20 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol NDUFA6   
Synonyms B14, CI-B14, LYRM6, NADHB14
Description NADH:ubiquinone oxidoreductase subunit A6
Transcript NM_002490   
Expression
Putative miRNA Targets on NDUFA6
3'UTR of NDUFA6
(miRNA target sites are highlighted)
>NDUFA6|NM_002490|3'UTR
   1 AGTCATTCAGTGGAAAGATGCACGTTGATACTATTTTAGAGCACAAATAAACTCACTATACAATGGTCACTTTGTGATAG
  81 AATTCCATTGATGAACCAATTTTCTGCAGCCTCTTTTTGCCTGAGCAAGTGGGACCTTGGTATACACATCACCTGTTCTT
 161 TCCCTTTTCTTGAAATGTGGTGTTTGCTGTAAATTGGATTGAATTATTTTTCTCAGAGCCTTGCATGTCAGTAAACAGGA
 241 AGGAAGGAGGCCATCTGTTCAAAAATTGTGACATTGGGCATGAGGCTGGACGACTTGTTAATGTAGTTGTTTCTATAGCC
 321 CCTATGATTAGACACTTCAGTGTCAGGGCACAGATTATCTAAAATTAGCCAGCTCTTCCTGTCAGGGATCAGGGCAGCAC
 401 TAACAGGCGCAAAAGTAGAGCTGTCAAGATGGGCTAGTTTTCCTTGGGTTCTGAAGAAAATGGATGTGCAAAGGCTTGGC
 481 TCCGCTACTTGTAACAAGCTCCAGCCCTTAAAATGAAATTAACTTCCTACTCAGGCACCCTGCTTAGGTGCACAGCTGTT
 561 CAATATACACAGAGAACAGAAATATTCCTACTGTCTTTGGAAACCCTGGTGTACCTCTCAGTGGCAGAATAAACATCAAC
 641 ACAGGTTCAAGAACTGAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' cuuaggaccaagacaggGAGUGAUc 5'
                           ||||||| 
Target 5' tttagagcacaaataaaCTCACTAt 3'
35 - 59 140.00 -7.90
2
miRNA  3' cuUAGGACCAAGACAGGGAGUGAUc 5'
            | ||||| | |  |:|||| |: 
Target 5' aaACCCTGG-TGTACCTCTCAGTGg 3'
601 - 624 118.00 -21.10
3
miRNA  3' cuUAGGACCAAGACAGG-------GAGUGAUc 5'
            | |||| ||  || |       :||| || 
Target 5' gcACCCTGCTTAGGTGCACAGCTGTTCAATAt 3'
535 - 566 107.00 -13.50
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN31523880 75 COSMIC
COSN31605911 80 COSMIC
COSN28187880 104 COSMIC
COSN21791854 390 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs200955834 3 dbSNP
rs200708307 10 dbSNP
rs765536300 17 dbSNP
rs1022762819 19 dbSNP
rs1191044263 22 dbSNP
rs145661734 23 dbSNP
rs768727500 24 dbSNP
rs1209672250 25 dbSNP
rs374848920 26 dbSNP
rs771519097 28 dbSNP
rs1222959079 29 dbSNP
rs955358791 33 dbSNP
rs745358070 34 dbSNP
rs372164861 39 dbSNP
rs770533454 40 dbSNP
rs764295396 46 dbSNP
rs1412525759 47 dbSNP
rs1332983242 58 dbSNP
rs1007055168 63 dbSNP
rs972722363 64 dbSNP
rs1212977238 91 dbSNP
rs1801381 94 dbSNP
rs1318081915 98 dbSNP
rs1268364156 108 dbSNP
rs1341298462 115 dbSNP
rs1252486396 119 dbSNP
rs1164950946 120 dbSNP
rs961800837 128 dbSNP
rs1484848527 129 dbSNP
rs996707000 133 dbSNP
rs1257005238 144 dbSNP
rs899336827 145 dbSNP
rs1191052547 150 dbSNP
rs1038520833 151 dbSNP
rs1373026282 155 dbSNP
rs1295354130 156 dbSNP
rs1170936912 160 dbSNP
rs1387752806 163 dbSNP
rs1005340813 166 dbSNP
rs560729062 168 dbSNP
rs1397281700 180 dbSNP
rs1315521779 183 dbSNP
rs1017003232 190 dbSNP
rs1324544330 194 dbSNP
rs1440723029 196 dbSNP
rs1006496549 198 dbSNP
rs187724944 199 dbSNP
rs530030412 203 dbSNP
rs1023527691 222 dbSNP
rs1345436891 225 dbSNP
rs1372759338 226 dbSNP
rs1232208753 231 dbSNP
rs1177029796 243 dbSNP
rs1012080958 247 dbSNP
rs1347327013 250 dbSNP
rs896398099 255 dbSNP
rs1192938442 260 dbSNP
rs947848375 272 dbSNP
rs1191023386 274 dbSNP
rs1251633869 280 dbSNP
rs1252080517 282 dbSNP
rs1177162156 285 dbSNP
rs1056095489 287 dbSNP
rs1480001114 291 dbSNP
rs934994390 292 dbSNP
rs902289662 302 dbSNP
rs1156366728 308 dbSNP
rs1256245219 312 dbSNP
rs374106146 312 dbSNP
rs563232761 315 dbSNP
rs1322507447 319 dbSNP
rs544559799 321 dbSNP
rs1368636808 327 dbSNP
rs1350593385 329 dbSNP
rs1386022410 337 dbSNP
rs7245 338 dbSNP
rs1237124304 340 dbSNP
rs114906790 361 dbSNP
rs1050011404 366 dbSNP
rs934266570 370 dbSNP
rs545892228 371 dbSNP
rs972755014 380 dbSNP
rs766065884 381 dbSNP
rs1395792431 382 dbSNP
rs762829084 390 dbSNP
rs755304213 397 dbSNP
rs984199167 404 dbSNP
rs557777752 405 dbSNP
rs533228811 408 dbSNP
rs1012031583 409 dbSNP
rs535482939 411 dbSNP
rs1472646085 413 dbSNP
rs1160665605 423 dbSNP
rs574468567 427 dbSNP
rs1457341786 428 dbSNP
rs556449731 443 dbSNP
rs1172757329 444 dbSNP
rs1293943874 453 dbSNP
rs1392578087 457 dbSNP
rs1389420914 462 dbSNP
rs1287987118 466 dbSNP
rs1440280952 473 dbSNP
rs1230303692 480 dbSNP
rs1365494212 481 dbSNP
rs999611794 482 dbSNP
rs537944892 484 dbSNP
rs1479883554 485 dbSNP
rs902150328 487 dbSNP
rs1005699960 495 dbSNP
rs1043866980 498 dbSNP
rs1183802014 503 dbSNP
rs570638819 505 dbSNP
rs1423213809 510 dbSNP
rs1003124812 513 dbSNP
rs1210657988 514 dbSNP
rs906217188 515 dbSNP
rs552042534 519 dbSNP
rs889530790 523 dbSNP
rs1237432401 536 dbSNP
rs1335317545 539 dbSNP
rs1411882181 540 dbSNP
rs1293967631 541 dbSNP
rs1416907779 542 dbSNP
rs1049962623 548 dbSNP
rs1378494806 550 dbSNP
rs948037068 553 dbSNP
rs1315469253 555 dbSNP
rs191842397 561 dbSNP
rs934130736 563 dbSNP
rs1275796292 566 dbSNP
rs1483213069 576 dbSNP
rs1206815390 577 dbSNP
rs1482655886 578 dbSNP
rs1053656203 580 dbSNP
rs1420100801 585 dbSNP
rs935167866 592 dbSNP
rs922879238 606 dbSNP
rs1037260247 615 dbSNP
rs1167790209 616 dbSNP
rs1390531961 617 dbSNP
rs1183693278 621 dbSNP
rs940034696 621 dbSNP
rs1431908529 628 dbSNP
rs1172045804 633 dbSNP
rs923980166 634 dbSNP
rs909845483 637 dbSNP
rs749608607 639 dbSNP
rs954683523 640 dbSNP
rs1434996172 654 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545216. RNA binding protein: AGO2. Condition:miR-124 transfection ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' cuuaggaccaagacaggGAGUGAUc 5'
                           ||||||| 
Target 5' uuuagagcacaaauaaaCUCACUAu 3'
8 - 32
Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 4700.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' cuuaggaccaagacaggGAGUGAUc 5'
                           ||||||| 
Target 5' uuuagagcacaaauaaaCUCACUAu 3'
9 - 33
Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
CLIP-seq Support 1 for dataset GSM545216
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / miR-124 transfection
Location of target site ENST00000602404.1 | 3UTR | AUACUAUUUUAGAGCACAAAUAAACUCACUAUACAAUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM714644
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repA
Location of target site ENST00000602404.1 | 3UTR | GAUACUAUUUUAGAGCACAAAUAAACUCACUAUACAAUGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
GSE38974 Chronic obstructive pulmonary disease -0.634 3.3e-4 -0.495 5.9e-3 25 Click to see details
GSE28544 Breast cancer 0.529 3.9e-3 0.548 2.8e-3 24 Click to see details
GSE28260 Renal cortex and medulla -0.593 1.6e-2 -0.582 1.8e-2 13 Click to see details
GSE19783 ER+ ER+ breast cancer -0.417 3.4e-2 -0.486 1.5e-2 20 Click to see details
GSE32688 Pancreatic cancer -0.246 8.7e-2 -0.230 1.0e-1 32 Click to see details
GSE42095 Differentiated embryonic stem cells 0.24 1.4e-1 0.217 1.6e-1 23 Click to see details
GSE19350 CNS germ cell tumors -0.309 1.6e-1 -0.455 6.9e-2 12 Click to see details
GSE17498 Multiple myeloma -0.101 2.7e-1 -0.090 2.9e-1 40 Click to see details
GSE34608 Pulmonary tuberculosis and sarcoidosis 0.132 2.9e-1 0.008 4.9e-1 20 Click to see details
GSE19783 ER- ER- breast cancer 0.018 4.4e-1 0.047 3.4e-1 79 Click to see details
GSE21687 Ependynoma primary tumors 0.012 4.6e-1 0.280 1.3e-2 64 Click to see details
GSE19536 Breast cancer -0.009 4.6e-1 -0.010 4.6e-1 100 Click to see details
GSE19536 Breast cancer -0.009 4.6e-1 -0.010 4.6e-1 100 Click to see details
GSE19536 Breast cancer -0.009 4.6e-1 -0.010 4.6e-1 100 Click to see details
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
63 hsa-miR-921 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT054764 ANGPTL1 angiopoietin like 1 3 1
MIRT066171 PIP4K2C phosphatidylinositol-5-phosphate 4-kinase type 2 gamma 2 2
MIRT069409 ZFYVE21 zinc finger FYVE-type containing 21 2 8
MIRT102284 DNAJB9 DnaJ heat shock protein family (Hsp40) member B9 2 4
MIRT107595 DNAJA1 DnaJ heat shock protein family (Hsp40) member A1 2 6
MIRT178618 HIAT1 major facilitator superfamily domain containing 14A 2 2
MIRT182407 TIPRL TOR signaling pathway regulator 2 4
MIRT186552 ZBTB18 zinc finger and BTB domain containing 18 2 2
MIRT273662 HOXC8 homeobox C8 2 2
MIRT283191 C16ORF52 chromosome 16 open reading frame 52 2 2
MIRT284890 NFAT5 nuclear factor of activated T-cells 5 2 2
MIRT347670 LSM14A LSM14A, mRNA processing body assembly factor 2 2
MIRT400222 SLC35F6 solute carrier family 35 member F6 2 2
MIRT403517 ASPH aspartate beta-hydroxylase 2 2
MIRT442251 DCTN5 dynactin subunit 5 2 2
MIRT443023 SDR39U1 short chain dehydrogenase/reductase family 39U member 1 2 2
MIRT443097 RNF20 ring finger protein 20 2 2
MIRT444560 TRA2B transformer 2 beta homolog 2 2
MIRT445696 PRKG1 protein kinase, cGMP-dependent, type I 2 2
MIRT454084 TMEM209 transmembrane protein 209 2 2
MIRT455463 LYPLA2 lysophospholipase II 2 2
MIRT456653 TIFA TRAF interacting protein with forkhead associated domain 2 2
MIRT458147 LYRM4 LYR motif containing 4 2 6
MIRT467073 SRRD SRR1 domain containing 2 4
MIRT467245 SPPL2A signal peptide peptidase like 2A 2 2
MIRT468246 SFXN4 sideroflexin 4 2 2
MIRT471589 PAQR5 progestin and adipoQ receptor family member 5 2 19
MIRT476639 G2E3 G2/M-phase specific E3 ubiquitin protein ligase 2 2
MIRT482433 ADM adrenomedullin 2 10
MIRT486848 PERP PERP, TP53 apoptosis effector 2 6
MIRT489656 SHMT1 serine hydroxymethyltransferase 1 2 2
MIRT493441 KANSL1 KAT8 regulatory NSL complex subunit 1 2 6
MIRT493841 FOXN3 forkhead box N3 2 4
MIRT501378 RBFOX2 RNA binding protein, fox-1 homolog 2 2 10
MIRT509679 ATAD5 ATPase family, AAA domain containing 5 2 4
MIRT510280 MED28 mediator complex subunit 28 2 2
MIRT512221 ATXN3 ataxin 3 2 6
MIRT514030 BNIP2 BCL2 interacting protein 2 2 2
MIRT521375 RDX radixin 2 4
MIRT521444 RAD51 RAD51 recombinase 2 2
MIRT526055 CBR1 carbonyl reductase 1 2 2
MIRT528658 FUNDC2 FUN14 domain containing 2 2 2
MIRT529975 TNFAIP8L1 TNF alpha induced protein 8 like 1 2 2
MIRT544098 IPMK inositol polyphosphate multikinase 2 2
MIRT545579 SNRPA1 small nuclear ribonucleoprotein polypeptide A' 2 2
MIRT547424 MED4 mediator complex subunit 4 2 2
MIRT548955 CD2AP CD2 associated protein 2 2
MIRT549537 NDUFA6 NADH:ubiquinone oxidoreductase subunit A6 2 4
MIRT552550 ZFP36L2 ZFP36 ring finger protein like 2 2 4
MIRT554640 ROBO1 roundabout guidance receptor 1 2 2
MIRT564904 YTHDF1 YTH N6-methyladenosine RNA binding protein 1 2 2
MIRT565578 SLC6A8 solute carrier family 6 member 8 2 2
MIRT568312 BAG4 BCL2 associated athanogene 4 2 2
MIRT617891 PTCHD3 patched domain containing 3 2 2
MIRT621892 TAF13 TATA-box binding protein associated factor 13 2 2
MIRT642850 RNF135 ring finger protein 135 2 2
MIRT665395 WEE1 WEE1 G2 checkpoint kinase 2 2
MIRT697879 UBE2B ubiquitin conjugating enzyme E2 B 2 2
MIRT698492 THOC2 THO complex 2 2 2
MIRT701227 OCRL OCRL, inositol polyphosphate-5-phosphatase 2 2
MIRT701872 MPLKIP M-phase specific PLK1 interacting protein 2 2
MIRT707045 TRPV2 transient receptor potential cation channel subfamily V member 2 2 2
MIRT715216 NPVF neuropeptide VF precursor 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-921 Progesterone approved 5994 Microarray Breast cancer 22330642 2012 up-regulated
miR-921 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-921 Fluorouracil 3385 NSC19893 approved sensitive High Pancreatic Cancer cell line (PANC-1)
hsa-miR-921 Gemcitabine 60750 NSC613327 approved sensitive High Pancreatic Cancer cell line (PANC-1)
hsa-miR-921 Gemcitabine 60750 NSC613327 approved resistant High Pancreatic Cancer cell line (PANC-1)
hsa-miR-921 Gemcitabine 60750 NSC613327 approved resistant High Pancreatic Cancer cell line (AsPC-1)
hsa-mir-921 Dabrafenib 44462760 NSC764134 approved sensitive cell line (A375)
hsa-mir-921 Androstenedione+Anastrozole resistant cell line (MCF-7)
hsa-mir-921 Cisplatin 5460033 NSC119875 approved sensitive cell line (KYSE)
hsa-mir-921 Fluorouracil 3385 NSC19893 approved sensitive cell line (OE19)
hsa-miR-921 Temozolomide 5394 NSC362856 approved sensitive cell line (U251)
hsa-miR-921 Doxorubicin 31703 NSC123127 approved resistant cell line (HS578T)
hsa-miR-921 Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-921 4-Hydroxytamoxifen+Tamoxifen resistant cell line (LY2)
hsa-miR-921 Ethanol+Tamoxifen resistant cell line (LY2)
hsa-miR-921 Fluorouracil 3385 NSC19893 approved resistant cell line (KM12C) (72 h)
hsa-miR-921 Sunitinib 5329102 NSC750690 approved resistant tissue (CardB)
hsa-miR-921 Pegylated interferon alpha+Ribavirin sensitive tissue (chronic hepatitis C)
hsa-miR-921 Oxaliplatin 6857599 NSC266046 approved resistant cell line (IGROV-1)
hsa-miR-921 Paclitaxel 36314 NSC125973 approved sensitive cell line (PC3PR20)
hsa-miR-921 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-921 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)
hsa-miR-921 Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR1)
hsa-miR-921 Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR4)

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