pre-miRNA Information
pre-miRNA hsa-mir-4666a   
Genomic Coordinates chr1: 228462074 - 228462152
Description Homo sapiens miR-4666a stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4666a-5p
Sequence 10| AUACAUGUCAGAUUGUAUGCC |30
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1288210208 3 dbSNP
rs1333314792 5 dbSNP
rs562096649 7 dbSNP
rs1324809033 10 dbSNP
rs1027635037 13 dbSNP
rs1351630778 15 dbSNP
rs1034879562 18 dbSNP
rs1383267208 21 dbSNP
Putative Targets

Gene Information
Gene Symbol ADH4   
Synonyms ADH-2, HEL-S-4
Description alcohol dehydrogenase 4 (class II), pi polypeptide
Transcript NM_000670   
Expression
Putative miRNA Targets on ADH4
3'UTR of ADH4
(miRNA target sites are highlighted)
>ADH4|NM_000670|3'UTR
   1 AGATGCCAGGAGCAATTCAGAATACTATCTGATTGAATGTGAACCTGCCTGGTTAATTTATTACCTGATTTGATGAACCA
  81 AGGAAAGCCATGAGTTTAAACAAATATTTACATTTAATATGGGAACATAAAAGAGCTTTAAATATTATAGACTTTGTACC
 161 TGTTATATATATGAATATTCCCTATGTTAAATAATAATAATAACTAGTGTTTATGAATAGAATCATATCATCTTTAGAAA
 241 TTGTTTAAAATTAGTTCTGGGAAGTTGAAAGTGGGGAATGAAGAGATAATAAATAAAACTAGATTGGCCATATGTTTATA
 321 ATTTTTTTAGATTGGGTAATGAATACATGGAGTTTCATTATACTTTTCTCTCCACTTTTGTCTATGTTGAAAATTTTCTG
 401 GGAGCTAAATGATGAGAACACATGGACACATGATGGGGAACAACACACACTGGGGCCTGTTGAGGGCAGGGAGTCGGCAG
 481 AGAGAGAGCATCAGGAAGAATAGCTAATGGATGCTGGGCTTCATACCTGGGTGATGAGATGATCTGTGCAGCAAAGCACC
 561 ATGGTACATGTTTACCTATGTAACAAACCTGCACATCCTGCACATGTACCCTGGAACTTAATAAAAGTTGGAAATTTTTA
 641 AAAAGAATGAATAAGACCTGGTATTTGATAGCACAACAGGGAGACTATAGTCAACAGCAATTTAATTGTATATTTTAATA
 721 TGACTAAAAGAGTATAATGGATTGTTTGTAACACAAATAAATGCTTGAGGAGATGGACACCCCAT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' ccGUAUGU-UA-GAC-UGUACAUa 5'
            | |:|| || ||| ||||||| 
Target 5' aaCCTGCACATCCTGCACATGTAc 3'
586 - 609 159.00 -11.30
2
miRNA  3' ccguaUGUUAGACUGUACAua 5'
               || ||   ||||||  
Target 5' aaagcACCATGGTACATGTtt 3'
553 - 573 128.00 -6.00
3
miRNA  3' ccgUAUGUUAGAC---UGUACAUa 5'
             |||| |||||   : ||||: 
Target 5' agaATACTATCTGATTGAATGTGa 3'
19 - 42 106.00 -12.00
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30480434 82 COSMIC
COSN1284059 88 COSMIC
COSN30111992 159 COSMIC
COSN25275192 372 COSMIC
COSN25550325 411 COSMIC
COSN22219920 466 COSMIC
COSN16758911 682 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1190269624 1 dbSNP
rs760320405 4 dbSNP
rs911441825 5 dbSNP
rs772910431 12 dbSNP
rs771623524 13 dbSNP
rs761625004 16 dbSNP
rs774035712 18 dbSNP
rs1042364 19 dbSNP
rs749221699 23 dbSNP
rs775708054 26 dbSNP
rs770056502 28 dbSNP
rs745959475 29 dbSNP
rs1243310718 32 dbSNP
rs1361067182 33 dbSNP
rs201456450 48 dbSNP
rs1488357703 49 dbSNP
rs962821830 52 dbSNP
rs1217267542 59 dbSNP
rs772181638 64 dbSNP
rs1017036821 67 dbSNP
rs1240031219 72 dbSNP
rs746376353 78 dbSNP
rs1334454619 91 dbSNP
rs1042365 93 dbSNP
rs1452953058 94 dbSNP
rs969935604 100 dbSNP
rs1173614882 101 dbSNP
rs29001238 114 dbSNP
rs1421628199 120 dbSNP
rs35490064 123 dbSNP
rs190646379 126 dbSNP
rs1276824468 127 dbSNP
rs1437372516 127 dbSNP
rs1372987093 128 dbSNP
rs1231770440 130 dbSNP
rs891434858 139 dbSNP
rs1302627956 148 dbSNP
rs978324790 156 dbSNP
rs185441044 157 dbSNP
rs1445916600 159 dbSNP
rs1318220130 160 dbSNP
rs376459922 173 dbSNP
rs1387901391 178 dbSNP
rs10097 185 dbSNP
rs992592781 188 dbSNP
rs904243514 192 dbSNP
rs1156898027 195 dbSNP
rs757727749 202 dbSNP
rs1406013523 203 dbSNP
rs1038779723 204 dbSNP
rs1172147304 206 dbSNP
rs1325834304 213 dbSNP
rs181464983 214 dbSNP
rs1438475969 216 dbSNP
rs1372050592 217 dbSNP
rs1377563209 218 dbSNP
rs1241181047 228 dbSNP
rs1277689758 248 dbSNP
rs757628953 259 dbSNP
rs573815821 261 dbSNP
rs973502633 264 dbSNP
rs889992433 271 dbSNP
rs1051765308 273 dbSNP
rs1332159577 306 dbSNP
rs963003106 308 dbSNP
rs1014829297 324 dbSNP
rs1180355384 329 dbSNP
rs1232782858 329 dbSNP
rs1473189637 335 dbSNP
rs545088297 336 dbSNP
rs934300296 344 dbSNP
rs1415982085 347 dbSNP
rs1434892999 348 dbSNP
rs1264927918 350 dbSNP
rs1171648361 361 dbSNP
rs918576670 363 dbSNP
rs1392896982 366 dbSNP
rs1326820406 374 dbSNP
rs56378607 381 dbSNP
rs1258972494 382 dbSNP
rs1030547400 383 dbSNP
rs941344103 385 dbSNP
rs1200809430 386 dbSNP
rs1294536253 387 dbSNP
rs1139489 407 dbSNP
rs996291980 411 dbSNP
rs1232369661 413 dbSNP
rs1269747631 414 dbSNP
rs1234588055 423 dbSNP
rs1258285583 424 dbSNP
rs1333346293 425 dbSNP
rs557221572 427 dbSNP
rs1194400498 432 dbSNP
rs909944040 433 dbSNP
rs749624891 434 dbSNP
rs1299567390 437 dbSNP
rs900559056 441 dbSNP
rs1443447334 451 dbSNP
rs1139490 452 dbSNP
rs1400516382 453 dbSNP
rs1255581710 455 dbSNP
rs936683195 459 dbSNP
rs970281955 464 dbSNP
rs1194718192 475 dbSNP
rs902506804 476 dbSNP
rs1463365542 482 dbSNP
rs1024163701 484 dbSNP
rs1395703908 491 dbSNP
rs993148695 502 dbSNP
rs1042699425 518 dbSNP
rs956014375 524 dbSNP
rs778122033 529 dbSNP
rs1172666529 538 dbSNP
rs1467457640 545 dbSNP
rs999795944 545 dbSNP
rs1353083161 547 dbSNP
rs1375887909 547 dbSNP
rs541210596 569 dbSNP
rs868802251 572 dbSNP
rs1192722302 585 dbSNP
rs1327789753 589 dbSNP
rs574014363 591 dbSNP
rs1225725567 597 dbSNP
rs949576486 600 dbSNP
rs1346330618 605 dbSNP
rs1213718007 611 dbSNP
rs918098909 620 dbSNP
rs1485409295 624 dbSNP
rs1217382509 626 dbSNP
rs144679012 628 dbSNP
rs938175541 640 dbSNP
rs1180741848 664 dbSNP
rs536292638 673 dbSNP
rs1372359149 674 dbSNP
rs1454538034 677 dbSNP
rs973169818 678 dbSNP
rs1373514648 689 dbSNP
rs1201113181 705 dbSNP
rs904212300 706 dbSNP
rs1347712858 707 dbSNP
rs1254791676 713 dbSNP
rs1234267081 718 dbSNP
rs1378073732 719 dbSNP
rs1311992815 726 dbSNP
rs1433590842 729 dbSNP
rs963055160 730 dbSNP
rs1017297193 740 dbSNP
rs1386700054 744 dbSNP
rs1380101020 747 dbSNP
rs1007336199 756 dbSNP
rs575607482 765 dbSNP
rs1372246240 770 dbSNP
rs2851249 771 dbSNP
rs1051305495 772 dbSNP
rs1334197487 776 dbSNP
rs954602165 782 dbSNP
rs539145343 783 dbSNP
rs1240987514 784 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 127.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' ccGUAUGU-UA-GAC-UGUACaua 5'
            | |:|| || ||| |||||   
Target 5' aaCCUGCACAUCCUGCACAUG--- 3'
6 - 26
Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
CLIP-seq Support 1 for dataset GSM714644
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repA
Location of target site ENST00000508393.1 | 3UTR | UAACAAACCUGCACAUCCUGCACAUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
83 hsa-miR-4666a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT057266 FAM35A family with sequence similarity 35 member A 2 2
MIRT059969 PATL1 PAT1 homolog 1, processing body mRNA decay factor 2 6
MIRT079031 TNRC6C trinucleotide repeat containing 6C 2 2
MIRT079631 DNAJB4 DnaJ heat shock protein family (Hsp40) member B4 2 2
MIRT086974 LANCL1 LanC like 1 2 2
MIRT091804 GOLGA4 golgin A4 2 2
MIRT229501 EIF1AX eukaryotic translation initiation factor 1A, X-linked 2 4
MIRT255972 WDR17 WD repeat domain 17 2 2
MIRT262975 ADO 2-aminoethanethiol dioxygenase 2 2
MIRT264774 PAFAH1B2 platelet activating factor acetylhydrolase 1b catalytic subunit 2 2 2
MIRT334169 CCND1 cyclin D1 2 6
MIRT345868 SRSF2 serine and arginine rich splicing factor 2 2 2
MIRT452477 DDX4 DEAD-box helicase 4 2 2
MIRT455764 TSPAN6 tetraspanin 6 2 4
MIRT461627 DCAF15 DDB1 and CUL4 associated factor 15 2 4
MIRT465169 TRPV2 transient receptor potential cation channel subfamily V member 2 2 4
MIRT468950 RPS14 ribosomal protein S14 2 6
MIRT483236 C2orf72 chromosome 2 open reading frame 72 2 8
MIRT498544 TMEM30B transmembrane protein 30B 2 2
MIRT500633 TXNIP thioredoxin interacting protein 2 4
MIRT504113 GPR158 G protein-coupled receptor 158 2 2
MIRT504428 ZNF85 zinc finger protein 85 2 6
MIRT505036 ZNF451 zinc finger protein 451 2 2
MIRT506526 MRPL17 mitochondrial ribosomal protein L17 2 6
MIRT508773 GSG1 germ cell associated 1 2 2
MIRT517297 ELF4 E74 like ETS transcription factor 4 2 6
MIRT519353 OBFC1 STN1, CST complex subunit 2 4
MIRT523891 ENPP6 ectonucleotide pyrophosphatase/phosphodiesterase 6 2 6
MIRT527509 ZNF134 zinc finger protein 134 2 2
MIRT531218 IFNGR2 interferon gamma receptor 2 2 2
MIRT535957 MOGAT1 monoacylglycerol O-acyltransferase 1 2 2
MIRT537229 GALNT7 polypeptide N-acetylgalactosaminyltransferase 7 2 4
MIRT537337 FKBP5 FK506 binding protein 5 2 2
MIRT539125 ARHGEF17 Rho guanine nucleotide exchange factor 17 2 2
MIRT539910 ISPD isoprenoid synthase domain containing 2 2
MIRT541527 MGAT4C MGAT4 family member C 2 2
MIRT546119 USP25 ubiquitin specific peptidase 25 2 2
MIRT548846 CERCAM cerebral endothelial cell adhesion molecule 2 2
MIRT549892 LINC00955 long intergenic non-protein coding RNA 955 2 2
MIRT549898 ADH4 alcohol dehydrogenase 4 (class II), pi polypeptide 2 2
MIRT550763 ENOX2 ecto-NOX disulfide-thiol exchanger 2 2 4
MIRT553200 UBE2A ubiquitin conjugating enzyme E2 A 2 2
MIRT553970 SRSF10 serine and arginine rich splicing factor 10 2 2
MIRT554092 SMU1 DNA replication regulator and spliceosomal factor 2 2
MIRT555208 PROX1 prospero homeobox 1 2 4
MIRT555834 PAX5 paired box 5 2 4
MIRT556865 JAZF1 JAZF zinc finger 1 2 2
MIRT558594 CREBL2 cAMP responsive element binding protein like 2 2 2
MIRT559690 AGO2 argonaute 2, RISC catalytic component 2 4
MIRT563312 ORC4 origin recognition complex subunit 4 2 2
MIRT563585 FAM229B family with sequence similarity 229 member B 2 2
MIRT563853 ALYREF Aly/REF export factor 2 4
MIRT565146 TUBB2A tubulin beta 2A class IIa 2 2
MIRT565769 SEPHS1 selenophosphate synthetase 1 2 2
MIRT568317 BACH1 BTB domain and CNC homolog 1 2 2
MIRT575387 Unc5b unc-5 netrin receptor B 2 4
MIRT607728 BDH1 3-hydroxybutyrate dehydrogenase 1 2 8
MIRT612691 PLXNA4 plexin A4 2 4
MIRT615916 GDPD1 glycerophosphodiester phosphodiesterase domain containing 1 2 2
MIRT629792 P2RY1 purinergic receptor P2Y1 2 2
MIRT632119 FKBP9 FK506 binding protein 9 2 2
MIRT645554 ZDHHC15 zinc finger DHHC-type containing 15 2 4
MIRT651479 WWC3 WWC family member 3 2 2
MIRT654138 RPH3A rabphilin 3A 2 6
MIRT655191 PHAX phosphorylated adaptor for RNA export 2 2
MIRT665543 UNC5B unc-5 netrin receptor B 2 5
MIRT668057 GRIK3 glutamate ionotropic receptor kainate type subunit 3 2 2
MIRT678978 CERS4 ceramide synthase 4 2 4
MIRT679124 RBM3 RNA binding motif (RNP1, RRM) protein 3 2 2
MIRT686776 AZF1 azoospermia factor 1 2 2
MIRT687144 PTPN12 protein tyrosine phosphatase, non-receptor type 12 2 2
MIRT691453 C21orf58 chromosome 21 open reading frame 58 2 2
MIRT691469 FAM98B family with sequence similarity 98 member B 2 2
MIRT697031 UHRF1BP1 UHRF1 binding protein 1 2 2
MIRT698401 TM9SF3 transmembrane 9 superfamily member 3 2 2
MIRT702056 RNMT RNA guanine-7 methyltransferase 2 2
MIRT705903 ADAM9 ADAM metallopeptidase domain 9 2 2
MIRT707491 MMADHC methylmalonic aciduria and homocystinuria, cblD type 2 2
MIRT708080 KLHL23 kelch like family member 23 2 2
MIRT709738 TRIM27 tripartite motif containing 27 2 2
MIRT710056 RWDD2A RWD domain containing 2A 2 2
MIRT710226 KCNK1 potassium two pore domain channel subfamily K member 1 2 2
MIRT712043 STYK1 serine/threonine/tyrosine kinase 1 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4666a Doxorubicin 31703 NSC123127 approved sensitive High Triple-Negative Breast Cancer cell line (MDA-MB-231, MDA-MB-468)

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