pre-miRNA Information
pre-miRNA hsa-mir-4457   
Genomic Coordinates chr5: 1309310 - 1309377
Description Homo sapiens miR-4457 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4457
Sequence 43| UCACAAGGUAUUGACUGGCGUA |64
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1031676297 8 dbSNP
rs1249861366 14 dbSNP
rs115769169 17 dbSNP
rs968846507 19 dbSNP
rs540870271 20 dbSNP
rs993130937 22 dbSNP
Putative Targets

Gene Information
Gene Symbol MOAP1   
Synonyms MAP-1, PNMA4
Description modulator of apoptosis 1
Transcript NM_022151   
Expression
Putative miRNA Targets on MOAP1
3'UTR of MOAP1
(miRNA target sites are highlighted)
>MOAP1|NM_022151|3'UTR
   1 GTCTCAGGGAACCACGAAGGGATATGGCAATGAGTAGAGCATGAAGGTAGAACAGTCTATATACTCTTGTGACACATACA
  81 ATCCCTACCTTGTGCTGCCAAGTAACTCATTTTTGTGCAATTCTCAGTATAAGCCCTTTGTCGTTTCTGTGCCTATTTAA
 161 AGTCTCCTAAAGGTGTAATTGACTAGGAAGGATGTAGTTCTACACTGCCATTTACCTATTTAAATTCATCCTTGTGAATA
 241 TCTTTGTTGTTGTTGTTGAGACAGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGCAT
 321 CCTCCGCCTTCCAGGTTTAAGCTATTCTCCTGCCTCAGTTGCCCGAGTAGCTGGGACTACAGGCATGTGCCACCACGCCC
 401 AGCTAAGTTTTGCATTATCAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTAGTTTCGAACTCCTGACCTCAAGTGAT
 481 CCACCTGCTTCGGCCTCTGAAAGTGCTGGGATTACAGGCGTGAGACACTGCGCCCAGCCTCATCCCTGTGAATTTCTTAT
 561 TGTACACAAGTTCTTTCACTATCTGTGTGCAGTGCTCTGAGGGGACAGACAAGGCTTGGGTGTATATGCCAACCAGATCT
 641 CATTGAAGTATCAGCTTGTTTGGTACTAGGTGCAAGTGTAGCATGTCACATGTGACCATGTTGGATCATTGACAATTTTT
 721 AGGTATGTACTGACCTACATTTATGATGAAGATCCTGAGCGGAGGTTAAGATATTAAGTTATTTTCCATATGAATCAGAA
 801 TTATATTGATTCTGTGCAATCAAAACAAAAGGCAGAATAGAATGCTGAGATTGGTTAAGTTTGCAATGACCATCTTGAAC
 881 CACAGATTTCTGCTATGTGTCATCAAAACATCTAGTTCTGAGTAACATTTTCACGATTGTTATAAAATTATAGGTGTGAA
 961 CTTCTAAAATAAAGGAATGCTAATAAAATGAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' augcgGUCAGUUAUGGAACACu 5'
               || ||  ||||||||| 
Target 5' acataCAATCCCTACCTTGTGc 3'
74 - 95 161.00 -15.00
2
miRNA  3' augcgGUCAGUUAU---GGAACACu 5'
               ||||| |||   :|||||| 
Target 5' tagaaCAGTCTATATACTCTTGTGa 3'
48 - 72 149.00 -19.20
3
miRNA  3' augcGGUCAGUU--A-U-GGAACACu 5'
              |:| |:||  | | ||||||| 
Target 5' ttacCTATTTAAATTCATCCTTGTGa 3'
212 - 237 148.00 -12.50
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30138112 16 COSMIC
COSN30159835 16 COSMIC
COSN5752227 36 COSMIC
COSN30171820 66 COSMIC
COSN30128401 95 COSMIC
COSN31600601 119 COSMIC
COSN1173085 178 COSMIC
COSN14815221 181 COSMIC
COSN31961024 271 COSMIC
COSN16598935 325 COSMIC
COSN26332055 488 COSMIC
COSN22404288 821 COSMIC
COSN8462197 899 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1034984701 2 dbSNP
rs745374087 3 dbSNP
rs748928852 6 dbSNP
rs1195867693 9 dbSNP
rs1461764749 11 dbSNP
rs371892869 13 dbSNP
rs1163027215 15 dbSNP
rs1405030325 16 dbSNP
rs752903110 19 dbSNP
rs756942904 21 dbSNP
rs751135476 27 dbSNP
rs1194543878 30 dbSNP
rs1046099 31 dbSNP
rs1247957469 32 dbSNP
rs193202063 39 dbSNP
rs752674243 40 dbSNP
rs1271701715 42 dbSNP
rs1218868335 43 dbSNP
rs1320765009 47 dbSNP
rs200675369 50 dbSNP
rs1477571363 56 dbSNP
rs1011529948 59 dbSNP
rs956225599 61 dbSNP
rs1031663664 74 dbSNP
rs553655848 78 dbSNP
rs115060758 81 dbSNP
rs755588291 88 dbSNP
rs1297643557 92 dbSNP
rs1324685272 93 dbSNP
rs1217471325 94 dbSNP
rs1049815987 120 dbSNP
rs997389242 134 dbSNP
rs1336450258 135 dbSNP
rs1211471131 136 dbSNP
rs1271607350 142 dbSNP
rs1256816465 143 dbSNP
rs898667050 144 dbSNP
rs15837 149 dbSNP
rs1319440400 160 dbSNP
rs1193009840 162 dbSNP
rs940320450 164 dbSNP
rs907518351 166 dbSNP
rs1163073946 169 dbSNP
rs1367612562 192 dbSNP
rs551449168 196 dbSNP
rs1290473566 197 dbSNP
rs1047404365 205 dbSNP
rs1312911984 208 dbSNP
rs1356839633 243 dbSNP
rs1231014464 246 dbSNP
rs754233121 256 dbSNP
rs911658408 259 dbSNP
rs1439169722 263 dbSNP
rs1317209845 264 dbSNP
rs531443689 270 dbSNP
rs541622149 271 dbSNP
rs985739739 274 dbSNP
rs931649457 275 dbSNP
rs113142191 276 dbSNP
rs978692368 278 dbSNP
rs967736533 280 dbSNP
rs112648887 281 dbSNP
rs970293083 282 dbSNP
rs1160983152 285 dbSNP
rs914444339 289 dbSNP
rs529132151 295 dbSNP
rs992668340 300 dbSNP
rs1174199137 301 dbSNP
rs956007609 313 dbSNP
rs1301810189 322 dbSNP
rs1373576387 326 dbSNP
rs1128801 331 dbSNP
rs1035119902 340 dbSNP
rs146054560 358 dbSNP
rs955382403 359 dbSNP
rs1028326855 364 dbSNP
rs187086208 365 dbSNP
rs1237020244 370 dbSNP
rs764538347 377 dbSNP
rs1458706935 387 dbSNP
rs1179191110 391 dbSNP
rs1038556236 396 dbSNP
rs1208909567 397 dbSNP
rs1194097799 401 dbSNP
rs1312440002 413 dbSNP
rs1279721842 415 dbSNP
rs1174705553 417 dbSNP
rs564506206 421 dbSNP
rs758845322 428 dbSNP
rs149552910 429 dbSNP
rs575324412 432 dbSNP
rs773848497 440 dbSNP
rs1375784499 441 dbSNP
rs1446492129 443 dbSNP
rs1309163256 448 dbSNP
rs555485680 455 dbSNP
rs1056736663 457 dbSNP
rs138223191 459 dbSNP
rs1379131838 460 dbSNP
rs1301202920 464 dbSNP
rs1447512742 465 dbSNP
rs182799322 467 dbSNP
rs1050123706 476 dbSNP
rs553355402 491 dbSNP
rs1214065733 492 dbSNP
rs1417631989 497 dbSNP
rs540147097 516 dbSNP
rs191843634 519 dbSNP
rs150416159 520 dbSNP
rs945949555 525 dbSNP
rs1167781211 529 dbSNP
rs1441474240 531 dbSNP
rs1461678501 532 dbSNP
rs1025204540 552 dbSNP
rs924497843 553 dbSNP
rs753836415 556 dbSNP
rs913165269 560 dbSNP
rs766516654 566 dbSNP
rs1180792844 577 dbSNP
rs1028361224 579 dbSNP
rs17128917 582 dbSNP
rs1216385637 583 dbSNP
rs959842138 587 dbSNP
rs1317484842 591 dbSNP
rs1214584547 593 dbSNP
rs1238810097 597 dbSNP
rs1274755889 614 dbSNP
rs1449661104 615 dbSNP
rs962801887 622 dbSNP
rs1487504696 642 dbSNP
rs1486835351 648 dbSNP
rs1017446377 665 dbSNP
rs774333070 672 dbSNP
rs1285767191 679 dbSNP
rs1475303978 685 dbSNP
rs1004348853 693 dbSNP
rs1241423480 698 dbSNP
rs1166661674 699 dbSNP
rs1420389099 701 dbSNP
rs927138954 708 dbSNP
rs887273862 709 dbSNP
rs1319278557 713 dbSNP
rs1311271078 715 dbSNP
rs1227782159 722 dbSNP
rs1380065901 723 dbSNP
rs1295339711 731 dbSNP
rs969879852 735 dbSNP
rs1027622846 739 dbSNP
rs568953077 741 dbSNP
rs995260614 742 dbSNP
rs1354585017 746 dbSNP
rs1035267580 750 dbSNP
rs1227509066 757 dbSNP
rs962247070 760 dbSNP
rs1318615366 764 dbSNP
rs1214393282 765 dbSNP
rs113523165 778 dbSNP
rs1359589602 792 dbSNP
rs1003831490 798 dbSNP
rs1009135483 803 dbSNP
rs576565267 804 dbSNP
rs1235366993 806 dbSNP
rs1472289629 817 dbSNP
rs1421384759 819 dbSNP
rs1409210780 830 dbSNP
rs1422004059 831 dbSNP
rs140853904 845 dbSNP
rs1348023705 847 dbSNP
rs1044089991 858 dbSNP
rs762744412 866 dbSNP
rs781472312 885 dbSNP
rs369008969 889 dbSNP
rs1447619160 893 dbSNP
rs535263702 895 dbSNP
rs566684082 896 dbSNP
rs1237781152 909 dbSNP
rs1285212649 909 dbSNP
rs1415404302 910 dbSNP
rs924489633 915 dbSNP
rs986673842 916 dbSNP
rs1348570831 926 dbSNP
rs769354501 934 dbSNP
rs921247854 935 dbSNP
rs975409686 937 dbSNP
rs1233751061 943 dbSNP
rs1217781930 948 dbSNP
rs1177225009 951 dbSNP
rs138104395 954 dbSNP
rs1435929279 955 dbSNP
rs1057058595 975 dbSNP
rs1400190050 989 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 64112.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' augcGGUCAGUU--A-U-GGAACacu 5'
              |:| |:||  | | |||||   
Target 5' uuacCUAUUUAAAUUCAUCCUUG--- 3'
12 - 34
Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
CLIP-seq Support 1 for dataset GSM4903829
Method / RBP HITS-CLIP / AGO
Cell line / Condition Human neurons / CTLTD_shCTL_a
Location of target site NM_022151 | 3UTR | CAUCCUUGUGAAUAUCU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161238
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4903833
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_a
Location of target site NM_022151 | 3UTR | AGGUAGAACAGUCUAUAUACUCUUGUGACACAUACAAUCCCUACCUUGUGCUGCCAAGUAACUCAUUUUUGUGCAAUUCUCAGUAUAAGCCCUUUGUCGUUUCUGUGCCUAUUUAAAGUCUCCUAAAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4903835
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_c
Location of target site NM_022151 | 3UTR | ACUCUUGUGACACAUACAAUCCCUACCUUGUGCUGCCAAGUAACUCAUUUUUGUGCAAUUCUCAGUAUAAGCCCUUUGUCGUUUCUGUGCCUAUUUAAAGUCUCCUAAAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM4903837
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_b
Location of target site NM_022151 | 3UTR | CCCUACCUUGUGCUGCCAAGUAACUCAUUUUUGUGCAAUUCUCAGUAUAAGCCCUUUGUCGUUUCUGUGCCUAUUUAAAGUCUCCUAAAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM4903838
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_c
Location of target site NM_022151 | 3UTR | ACACAUACAAUCCCUACCUUGUGCUGCCAAGUAACUCAUUUUUGUGCAAUUCUCAGUAUAAGCCCUUUGUCGUUUCUGUGCCUAUUUAAAGUCUCCUAAAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM714644
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repA
Location of target site ENST00000298894.4 | 3UTR | UACACUGCCAUUUACCUAUUUAAAUUCAUCCUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
83 hsa-miR-4457 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT098032 SOBP sine oculis binding protein homolog 2 2
MIRT202580 PCMTD2 protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2 2 6
MIRT247137 WEE1 WEE1 G2 checkpoint kinase 2 4
MIRT349266 PTBP1 polypyrimidine tract binding protein 1 2 2
MIRT363756 EIF4EBP1 eukaryotic translation initiation factor 4E binding protein 1 2 2
MIRT443586 FAM84B family with sequence similarity 84 member B 2 2
MIRT452333 EIF5AL1 eukaryotic translation initiation factor 5A-like 1 2 2
MIRT453864 ZBTB40 zinc finger and BTB domain containing 40 2 4
MIRT471486 PDE4D phosphodiesterase 4D 2 4
MIRT476290 GMFB glia maturation factor beta 2 8
MIRT479897 CCDC117 coiled-coil domain containing 117 2 4
MIRT484251 ANK1 ankyrin 1 2 2
MIRT491473 TMEM214 transmembrane protein 214 2 2
MIRT493804 GAN gigaxonin 2 6
MIRT499252 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT502271 HNRNPA1 heterogeneous nuclear ribonucleoprotein A1 2 4
MIRT504651 RPL9 ribosomal protein L9 2 6
MIRT505211 UBN2 ubinuclein 2 2 8
MIRT508952 SNRPB small nuclear ribonucleoprotein polypeptides B and B1 2 4
MIRT512691 POP1 POP1 homolog, ribonuclease P/MRP subunit 2 2
MIRT513320 SCUBE3 signal peptide, CUB domain and EGF like domain containing 3 2 2
MIRT513870 HOXA5 homeobox A5 2 2
MIRT517342 ZNF529 zinc finger protein 529 2 4
MIRT518947 LSG1 large 60S subunit nuclear export GTPase 1 2 2
MIRT520867 SUGT1 SGT1 homolog, MIS12 kinetochore complex assembly cochaperone 2 2
MIRT528325 GIGYF2 GRB10 interacting GYF protein 2 2 2
MIRT531990 SLCO1B3 solute carrier organic anion transporter family member 1B3 2 2
MIRT533298 USP46 ubiquitin specific peptidase 46 2 2
MIRT545257 TRIM36 tripartite motif containing 36 2 4
MIRT547038 POGZ pogo transposable element derived with ZNF domain 2 2
MIRT556103 MOAP1 modulator of apoptosis 1 2 2
MIRT558321 DR1 down-regulator of transcription 1 2 2
MIRT558521 CSRNP3 cysteine and serine rich nuclear protein 3 2 2
MIRT560906 TMED10 transmembrane p24 trafficking protein 10 2 2
MIRT568448 ARPP19 cAMP regulated phosphoprotein 19 2 2
MIRT570586 OTUD7B OTU deubiquitinase 7B 2 2
MIRT572799 SIGLEC14 sialic acid binding Ig like lectin 14 2 2
MIRT573863 C9orf78 chromosome 9 open reading frame 78 2 2
MIRT575058 P2ry1 purinergic receptor P2Y, G-protein coupled 1 2 5
MIRT609931 SLC38A1 solute carrier family 38 member 1 2 4
MIRT610836 ZNF585A zinc finger protein 585A 2 4
MIRT611474 P2RY1 purinergic receptor P2Y1 2 7
MIRT613569 YY2 YY2 transcription factor 2 2
MIRT618626 GREB1 growth regulation by estrogen in breast cancer 1 2 2
MIRT620606 SAP30 Sin3A associated protein 30 2 2
MIRT621017 CLSTN3 calsyntenin 3 2 4
MIRT635314 FAM179A TOG array regulator of axonemal microtubules 2 2 2
MIRT635919 GLTSCR2 NOP53 ribosome biogenesis factor 2 2
MIRT640598 TM9SF4 transmembrane 9 superfamily member 4 2 2
MIRT641784 YWHAB tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta 2 4
MIRT644067 IQCE IQ motif containing E 2 2
MIRT648288 TRAPPC2L trafficking protein particle complex 2 like 2 2
MIRT658085 FOXR2 forkhead box R2 2 2
MIRT659077 DEPTOR DEP domain containing MTOR interacting protein 2 2
MIRT665307 ZBTB37 zinc finger and BTB domain containing 37 2 2
MIRT665975 SYTL4 synaptotagmin like 4 2 2
MIRT666302 SLC25A25 solute carrier family 25 member 25 2 2
MIRT674906 RASSF9 Ras association domain family member 9 2 2
MIRT680086 THAP1 THAP domain containing 1 2 2
MIRT681488 DIP2A disco interacting protein 2 homolog A 2 2
MIRT691244 DFNB59 pejvakin 2 2
MIRT692362 AGTRAP angiotensin II receptor associated protein 2 2
MIRT693035 MB21D1 Mab-21 domain containing 1 2 2
MIRT693837 STAT5A signal transducer and activator of transcription 5A 2 2
MIRT694479 LRTOMT leucine rich transmembrane and O-methyltransferase domain containing 2 2
MIRT696070 ZNF264 zinc finger protein 264 2 2
MIRT696578 TTC21B tetratricopeptide repeat domain 21B 2 2
MIRT696760 MTFMT mitochondrial methionyl-tRNA formyltransferase 2 2
MIRT697307 ZNF652 zinc finger protein 652 2 2
MIRT700152 RNF115 ring finger protein 115 2 2
MIRT701056 PARP2 poly(ADP-ribose) polymerase 2 2 2
MIRT701198 OTUD3 OTU deubiquitinase 3 2 2
MIRT701335 NSD1 nuclear receptor binding SET domain protein 1 2 2
MIRT702656 ITGA3 integrin subunit alpha 3 2 2
MIRT703618 FBXO45 F-box protein 45 2 2
MIRT704673 CHTOP chromatin target of PRMT1 2 2
MIRT708894 ZNF780A zinc finger protein 780A 2 2
MIRT711620 DGKH diacylglycerol kinase eta 2 2
MIRT713745 TMEM81 transmembrane protein 81 2 2
MIRT719712 CD101 CD101 molecule 2 2
MIRT720294 DLGAP3 DLG associated protein 3 2 2
MIRT722606 CCDC152 coiled-coil domain containing 152 2 2
MIRT724566 ACSBG1 acyl-CoA synthetase bubblegum family member 1 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4457 Dabrafenib 44462760 NSC764134 approved sensitive cell line (A375)
hsa-miR-4457 Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-4457 Osimertinib 71496458 NSC779217 approved sensitive cell line (PC9)
hsa-miR-4457 Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)

Error report submission