pre-miRNA Information | |
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pre-miRNA | hsa-mir-3655 |
Genomic Coordinates | chr5: 140647844 - 140647926 |
Description | Homo sapiens miR-3655 stem-loop |
Comment | None |
RNA Secondary Structure |
Mature miRNA Information | |||||||||||||||||||||||||||||||
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Mature miRNA | hsa-miR-3655 | ||||||||||||||||||||||||||||||
Sequence | 1| GCUUGUCGCUGCGGUGUUGCU |21 | ||||||||||||||||||||||||||||||
Evidence | Experimental | ||||||||||||||||||||||||||||||
Experiments | 454 | DRVs in miRNA |
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SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | CNEP1R1 | ||||||||||||||||||||
Synonyms | C16orf69, NEP1-R1, NEP1R1, TMEM188, TMP125 | ||||||||||||||||||||
Description | CTD nuclear envelope phosphatase 1 regulatory subunit 1 | ||||||||||||||||||||
Transcript | NM_153261 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on CNEP1R1 | |||||||||||||||||||||
3'UTR of CNEP1R1 (miRNA target sites are highlighted) |
>CNEP1R1|NM_153261|3'UTR 1 CAATCTTCACTCATTGTTATGGGACTTAAAATAGCCTTTCTTCGAATAAGTGATACAGCAAAAAGCCATAAAGGATTCCT 81 TTTGCGGTTGGATATGTAAAGGTCATAGCAGCAACTGACAAGAAGTGTGCAATATTTACCTGGATTATCTTGATGATGGT 161 GACTCATTATCAGTGCTTTGGTACTTTTGATTACCTGTGTTTCAGTATTAGTGTCACTTTAGTACTTCAGATCCTGCAAA 241 TATTTTTGCAGATGAAGTATGTATGTATGTTACTAAGTTAAACTTAGAAACAGAACCTCATTCAGTTTTTATAATGTATT 321 TTTGCAAACTACTGTAAATAGCAAATCAATGCCAATGTTAAACAAAGAGGAAAACGTTGTGTGGACTTTGTTCTCTTGCA 401 CCAGTATTTCAGGAACATCTGCTTGCCATCCCCACAGCTCTTTAAAACTGGCTATTATGTGTGCCTTTCATTCTTACATT 481 TCTAATCATACTGCAGGAAAAACATTGGATTCAGCTTAGACTGAGGAAAACTCTCCATTATGTTGTAAGAAATTATAGAT 561 GTTTTGAGAGACACTTTTTGTTAAACCAGATATTGAACTCCAGCAACTATTGTGGTTATATTTTTAGTTCATTGTTCTCA 641 TTTAATGCTAAATATCCTTTATATTGCTTTAATAATTTTCTTTTTTTTTTTTTTTTTTTTAGACGGAGTCTCGCTCTGTT 721 GCCAGGCTGGAGGGCAGTGGCACGATCTTGGCTTTCTGCAACCTCTGCCTCCCAGGTTCAAGCGATTCTCCTGCTTCAGC 801 CTTCTGAGTAGCTGGGACTACAGGCGCATGCCACCATGCCCAGCTAATTTTTTTGTATTTTTAGTAGAGACGGGGTTTCA 881 CCACGTTGGCCAGGATGGTTTCGATCTCCTGACCTCGTGATCCTCCTGCCTCATCCTCCCAAAATGCTGGGATTACAGGC 961 ATAAGCCACCGTGCCTGGCCTCTTTAATAATTTTTAAAATACCCTAAAGGCTTGTGAATATACAAGTCTACTGATAAATT 1041 ATGTATTGTCTGGGAATTTGATAGTCATTGTTTTAGATAACTGGATTTTACGCTGTGGTAGACAGGCTGTGACACTAGTG 1121 TTGCACAGGTGTAATTGGTCATCCTATGCCTTCACCAGAATAACTTGGGAGTGGTGCCAGAAACTAGAGTCTACAATTCT 1201 CACTGTTTAGAGAGTGTTAATGACATACTGTGTATGCATAATAGCCGCATGTACTATAATAGCCCTTAAAATTAAACTAT 1281 TGGGATTGCTGTAAATATTTTAAAGTACTGGAGGTGCCTTTTACCTGTTTATTAGATTTTGAAAAGGTTTAAATTATTTC 1361 ATGAGCAATCTTTTAAATTTCATTTAACATAAAGCTGAAAATTCAATAACAGGATAAAAAAGCTTTTTAACAAGGCTGCC 1441 ATTTAACTTAAATGTGTTCATCTTAGCTTTCACTTGTATAAAATTTGATTCTTTGAACTGCAGCAATAAAACCCTCAGCT 1521 CCTAAGAAGTCTTAAGAGGGTATTCTATATATTCTGCTTTGTTTTATTTTCTGTAAATTTTGTAGGTAAATATGTGCATT 1601 AAAAATAAATACTTTATATATAACTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | HEK293 |
Disease | 255919.0 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1
... - Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods. |
Article |
- Kishore S; Jaskiewicz L; Burger L; Hausser et al. - Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
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CLIP-seq Support 1 for dataset GSM714644 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | HEK293 / completeT1, repA |
Location of target site | ENST00000458059.3 | 3UTR | AAGUGUGCAAUAUUUACCUGGAUUAUCUUGAUG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21572407 / GSE28865 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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32 hsa-miR-3655 Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT446335 | ATP5C1 | ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 | 2 | 2 | ||||||||
MIRT476874 | FEM1B | fem-1 homolog B | 2 | 2 | ||||||||
MIRT500976 | SPPL2A | signal peptide peptidase like 2A | 2 | 4 | ||||||||
MIRT558681 | CNEP1R1 | CTD nuclear envelope phosphatase 1 regulatory subunit 1 | 2 | 2 | ||||||||
MIRT617898 | PTCHD3 | patched domain containing 3 | 2 | 2 | ||||||||
MIRT620111 | HARBI1 | harbinger transposase derived 1 | 2 | 2 | ||||||||
MIRT620647 | CXCL5 | C-X-C motif chemokine ligand 5 | 2 | 2 | ||||||||
MIRT630797 | FAM46A | family with sequence similarity 46 member A | 2 | 2 | ||||||||
MIRT632226 | WDR37 | WD repeat domain 37 | 2 | 2 | ||||||||
MIRT638561 | KCNJ10 | potassium voltage-gated channel subfamily J member 10 | 2 | 2 | ||||||||
MIRT639110 | MMAB | methylmalonic aciduria (cobalamin deficiency) cblB type | 2 | 2 | ||||||||
MIRT640352 | C1orf210 | chromosome 1 open reading frame 210 | 2 | 2 | ||||||||
MIRT642520 | CNTROB | centrobin, centriole duplication and spindle assembly protein | 2 | 2 | ||||||||
MIRT642646 | PTGR2 | prostaglandin reductase 2 | 2 | 2 | ||||||||
MIRT643505 | ZNF28 | zinc finger protein 28 | 2 | 2 | ||||||||
MIRT645690 | TBC1D13 | TBC1 domain family member 13 | 2 | 2 | ||||||||
MIRT645936 | HIPK1 | homeodomain interacting protein kinase 1 | 2 | 2 | ||||||||
MIRT647643 | FAIM2 | Fas apoptotic inhibitory molecule 2 | 2 | 2 | ||||||||
MIRT648513 | PIGG | phosphatidylinositol glycan anchor biosynthesis class G | 2 | 2 | ||||||||
MIRT649163 | IQSEC1 | IQ motif and Sec7 domain 1 | 2 | 2 | ||||||||
MIRT651442 | XKR4 | XK related 4 | 2 | 2 | ||||||||
MIRT654151 | RPAP2 | RNA polymerase II associated protein 2 | 2 | 2 | ||||||||
MIRT657780 | GLIS3 | GLIS family zinc finger 3 | 2 | 2 | ||||||||
MIRT661242 | ARL17B | ADP ribosylation factor like GTPase 17B | 2 | 2 | ||||||||
MIRT664082 | METTL2B | methyltransferase like 2B | 2 | 2 | ||||||||
MIRT671672 | HEYL | hes related family bHLH transcription factor with YRPW motif-like | 2 | 2 | ||||||||
MIRT672199 | F2 | coagulation factor II, thrombin | 2 | 2 | ||||||||
MIRT693599 | SLC39A1 | solute carrier family 39 member 1 | 2 | 2 | ||||||||
MIRT714567 | GALNT10 | polypeptide N-acetylgalactosaminyltransferase 10 | 2 | 2 | ||||||||
MIRT721589 | LRRC2 | leucine rich repeat containing 2 | 2 | 2 | ||||||||
MIRT722888 | MOB3A | MOB kinase activator 3A | 2 | 2 | ||||||||
MIRT724762 | PSG4 | pregnancy specific beta-1-glycoprotein 4 | 2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||
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