pre-miRNA Information
pre-miRNA hsa-mir-3622b   
Genomic Coordinates chr8: 27701673 - 27701767
Description Homo sapiens miR-3622b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3622b-3p
Sequence 58| UCACCUGAGCUCCCGUGCCUG |78
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1232922758 2 dbSNP
rs530939348 5 dbSNP
rs1439657384 10 dbSNP
rs1157333896 12 dbSNP
rs66683138 14 dbSNP
rs1044808718 15 dbSNP
rs946438979 19 dbSNP
rs551845360 21 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol DCTN6   
Synonyms WS-3, WS3, p27
Description dynactin subunit 6
Transcript NM_006571   
Expression
Putative miRNA Targets on DCTN6
3'UTR of DCTN6
(miRNA target sites are highlighted)
>DCTN6|NM_006571|3'UTR
   1 GAACAGTGTATAACATGAAGATAACATTTTGTCTTTGACCACTGTCTTTTGAATGGGCCCACAGTGTTTATGTACTCTTA
  81 ACAACTCACAGAATAATACATGTTCACTTTATTTTGTAAAATTGGGTTGAGAGGAAACTAATGGAGTTTCATTGTAACTG
 161 TCCTTTGTAATTTATATAAATGTATTATTTTCCTATATCCTTGGTTCTTTTCTGATAATTTACAGATTTAGCTTTTCTTT
 241 TGTTATATAAACTGCTAGCCACAAATTTTAGTTATGTAAAAGGCTACCCTTGACAAGAAAAGACATACTGTCATGTATTT
 321 ATATTCTAGCATAGACTAAACTGAATAAAAATGCTGATAACAGGACCTTTAGTAAGCATACTTTAATCAGTTTTTCTCCT
 401 TGAATTAATCTTTTCTCTTAATTTTTATTATGTACTAGTTGTATTAAAGCTAATGTGTTTACTTTT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gucCGUGCCCUCGAGUCCACu 5'
             |:|:   |||| | ||| 
Target 5' gttGTATTAAAGCTAATGTGt 3'
438 - 458 94.00 -6.80
2
miRNA  3' guccgugcCCUC-GAGUccacu 5'
                  |||| :|||     
Target 5' aaactaatGGAGTTTCAttgta 3'
135 - 156 73.00 -6.30
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSM3412969 1 COSMIC
COSN31525792 20 COSMIC
COSN31528728 34 COSMIC
COSN30454543 40 COSMIC
COSN30116944 58 COSMIC
COSN31581116 60 COSMIC
COSN30108786 62 COSMIC
COSN30132370 69 COSMIC
COSN30162767 78 COSMIC
COSN28666104 135 COSMIC
COSN31601398 164 COSMIC
COSN6920773 346 COSMIC
COSN18816047 400 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1333770349 1 dbSNP
rs766879862 5 dbSNP
rs1280063980 8 dbSNP
rs200863630 9 dbSNP
rs1310620788 10 dbSNP
rs371971868 11 dbSNP
rs766433518 12 dbSNP
rs768192496 13 dbSNP
rs753137193 14 dbSNP
rs760861658 15 dbSNP
rs1417690615 22 dbSNP
rs1420377689 25 dbSNP
rs764035942 26 dbSNP
rs112297965 27 dbSNP
rs754123310 32 dbSNP
rs1176030211 40 dbSNP
rs757357787 48 dbSNP
rs1011337503 54 dbSNP
rs1244198606 60 dbSNP
rs1237863035 71 dbSNP
rs1460226521 78 dbSNP
rs546015805 87 dbSNP
rs537540664 90 dbSNP
rs1216736603 91 dbSNP
rs905131961 98 dbSNP
rs1186076613 100 dbSNP
rs1003374765 101 dbSNP
rs1034883335 103 dbSNP
rs1304951305 125 dbSNP
rs1239408546 135 dbSNP
rs1383454522 142 dbSNP
rs966586560 146 dbSNP
rs3808483 152 dbSNP
rs1401175546 154 dbSNP
rs1158065920 168 dbSNP
rs1406478670 182 dbSNP
rs1157720942 185 dbSNP
rs567949359 194 dbSNP
rs1386418596 201 dbSNP
rs993205160 208 dbSNP
rs1027832719 212 dbSNP
rs1177368301 225 dbSNP
rs936365706 227 dbSNP
rs1054875774 229 dbSNP
rs1375253010 233 dbSNP
rs1486964589 240 dbSNP
rs951734103 240 dbSNP
rs986226106 244 dbSNP
rs916356725 247 dbSNP
rs533864983 255 dbSNP
rs1215989750 257 dbSNP
rs1413250918 274 dbSNP
rs553622133 276 dbSNP
rs1046514943 283 dbSNP
rs1296026731 289 dbSNP
rs964298844 289 dbSNP
rs576642649 301 dbSNP
rs1359450245 305 dbSNP
rs1197361798 307 dbSNP
rs866160006 311 dbSNP
rs139694211 318 dbSNP
rs1424767085 327 dbSNP
rs1366868758 328 dbSNP
rs1049150419 332 dbSNP
rs1423282764 332 dbSNP
rs935521635 335 dbSNP
rs1347859961 340 dbSNP
rs1194363534 348 dbSNP
rs1430595283 367 dbSNP
rs558632646 373 dbSNP
rs1193159083 397 dbSNP
rs1490548759 403 dbSNP
rs1271103249 411 dbSNP
rs1052443227 421 dbSNP
rs1355544479 426 dbSNP
rs149784790 456 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545217. RNA binding protein: AGO2. Condition:miR-7 transfection ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' guccgugcccUCGAGUCCACu 5'
                    | :||||||| 
Target 5' ----uuuaaaAUUUCAGGUGa 3'
1 - 17
Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
CLIP-seq Support 1 for dataset GSM545217
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / miR-7 transfection
Location of target site ENST00000221114.3 | 3UTR | UUUAAAAUUUCAGGUGAAUUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
THCA -0.282 0.27 -0.429 0.17 7 Click to see details
THCA -0.282 0.27 -0.429 0.17 7 Click to see details
THCA -0.282 0.27 -0.429 0.17 7 Click to see details
THCA -0.282 0.27 -0.429 0.17 7 Click to see details
THCA -0.282 0.27 -0.429 0.17 7 Click to see details
THCA -0.282 0.27 -0.429 0.17 7 Click to see details
102 hsa-miR-3622b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT076713 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 2
MIRT080212 PRKACB protein kinase cAMP-activated catalytic subunit beta 2 2
MIRT081398 GTPBP3 GTP binding protein 3, mitochondrial 2 2
MIRT107158 ZBTB43 zinc finger and BTB domain containing 43 2 2
MIRT254071 BACH1 BTB domain and CNC homolog 1 2 2
MIRT409790 FOXO3 forkhead box O3 2 2
MIRT448679 MAPK9 mitogen-activated protein kinase 9 2 2
MIRT450206 ABHD15 abhydrolase domain containing 15 2 2
MIRT486621 PDK3 pyruvate dehydrogenase kinase 3 2 2
MIRT489267 TTLL1 tubulin tyrosine ligase like 1 2 2
MIRT493117 MKNK2 MAP kinase interacting serine/threonine kinase 2 2 4
MIRT494570 BAK1 BCL2 antagonist/killer 1 2 2
MIRT497395 RALY RALY heterogeneous nuclear ribonucleoprotein 2 2
MIRT504900 CCDC86 coiled-coil domain containing 86 2 2
MIRT505192 USP46 ubiquitin specific peptidase 46 2 4
MIRT506522 MSANTD4 Myb/SANT DNA binding domain containing 4 with coiled-coils 2 6
MIRT508162 ABCC5 ATP binding cassette subfamily C member 5 2 8
MIRT508545 PARVG parvin gamma 2 4
MIRT509850 BIRC5 baculoviral IAP repeat containing 5 2 6
MIRT510088 PPWD1 peptidylprolyl isomerase domain and WD repeat containing 1 2 8
MIRT512094 CRK CRK proto-oncogene, adaptor protein 2 4
MIRT515381 RPL7 ribosomal protein L7 2 2
MIRT519176 SCO1 SCO1, cytochrome c oxidase assembly protein 2 2
MIRT519964 ZCCHC8 zinc finger CCHC-type containing 8 2 2
MIRT522292 NKAP NFKB activating protein 2 2
MIRT523160 HMGB2 high mobility group box 2 2 4
MIRT525314 FANCA Fanconi anemia complementation group A 2 2
MIRT528194 PLEKHM2 pleckstrin homology and RUN domain containing M2 2 2
MIRT532885 ZNF451 zinc finger protein 451 2 2
MIRT538209 CYR61 cysteine rich angiogenic inducer 61 2 2
MIRT539497 ACTN4 actinin alpha 4 2 2
MIRT554600 RRAGC Ras related GTP binding C 2 2
MIRT562444 DCTN6 dynactin subunit 6 2 2
MIRT562748 AOC3 amine oxidase, copper containing 3 2 2
MIRT565798 SEC14L5 SEC14 like lipid binding 5 2 2
MIRT565838 SCML2 Scm polycomb group protein like 2 2 2
MIRT566072 RCC2 regulator of chromosome condensation 2 2 2
MIRT566104 RBPJ recombination signal binding protein for immunoglobulin kappa J region 2 2
MIRT572407 MRPS14 mitochondrial ribosomal protein S14 2 2
MIRT576198 Vsig2 V-set and immunoglobulin domain containing 2 2 2
MIRT576313 Acbd7 acyl-Coenzyme A binding domain containing 7 2 2
MIRT576651 Mill2 MHC I like leukocyte 2 1 1
MIRT576859 Socs6 suppressor of cytokine signaling 6 2 2
MIRT606819 BICD2 BICD cargo adaptor 2 2 2
MIRT610738 NUDT16 nudix hydrolase 16 2 4
MIRT614798 RORA RAR related orphan receptor A 2 2
MIRT619006 NTMT1 N-terminal Xaa-Pro-Lys N-methyltransferase 1 2 2
MIRT619420 NOS1AP nitric oxide synthase 1 adaptor protein 2 2
MIRT620405 MYO1H myosin IH 2 2
MIRT624869 ABHD13 abhydrolase domain containing 13 2 2
MIRT633527 ZFP30 ZFP30 zinc finger protein 2 2
MIRT633927 DNAH9 dynein axonemal heavy chain 9 2 2
MIRT636611 CLIC5 chloride intracellular channel 5 2 2
MIRT640616 MIOX myo-inositol oxygenase 2 2
MIRT642605 C14orf180 chromosome 14 open reading frame 180 2 2
MIRT644624 SRSF2 serine and arginine rich splicing factor 2 2 2
MIRT655113 PHLDA3 pleckstrin homology like domain family A member 3 2 2
MIRT658976 DNAJB5 DnaJ heat shock protein family (Hsp40) member B5 2 4
MIRT661495 CHMP1B charged multivesicular body protein 1B 2 2
MIRT662996 TMEM59 transmembrane protein 59 2 2
MIRT663075 SFR1 SWI5 dependent homologous recombination repair protein 1 2 2
MIRT665405 WEE1 WEE1 G2 checkpoint kinase 2 2
MIRT666106 SSR1 signal sequence receptor subunit 1 2 2
MIRT669562 ALDOA aldolase, fructose-bisphosphate A 2 2
MIRT670070 ZNF783 zinc finger family member 783 2 2
MIRT671192 ZNF891 zinc finger protein 891 2 2
MIRT675361 KLHL26 kelch like family member 26 2 2
MIRT678857 LINC00598 long intergenic non-protein coding RNA 598 2 2
MIRT679443 C19orf52 translocase of inner mitochondrial membrane 29 2 2
MIRT684163 ALDH1B1 aldehyde dehydrogenase 1 family member B1 2 2
MIRT684543 ZNF460 zinc finger protein 460 2 2
MIRT684674 SLC2A11 solute carrier family 2 member 11 2 2
MIRT684971 MINOS1 mitochondrial inner membrane organizing system 1 2 2
MIRT686006 NEK4 NIMA related kinase 4 2 2
MIRT687758 KIAA1328 KIAA1328 2 2
MIRT688961 ATXN3 ataxin 3 2 2
MIRT689409 UQCR11 ubiquinol-cytochrome c reductase, complex III subunit XI 2 2
MIRT690001 MMP17 matrix metallopeptidase 17 2 2
MIRT690165 ELP3 elongator acetyltransferase complex subunit 3 2 2
MIRT690202 C5orf45 MRN complex interacting protein 2 2
MIRT690477 ZNF33A zinc finger protein 33A 2 2
MIRT690566 MICA MHC class I polypeptide-related sequence A 2 2
MIRT692160 C10orf111 chromosome 10 open reading frame 111 2 2
MIRT693430 PLGLB2 plasminogen-like B2 2 2
MIRT693547 ZNF708 zinc finger protein 708 2 2
MIRT693697 PLGLB1 plasminogen-like B1 2 2
MIRT695006 HSPA6 heat shock protein family A (Hsp70) member 6 2 2
MIRT698525 TFRC transferrin receptor 2 2
MIRT699574 SIKE1 suppressor of IKBKE 1 2 2
MIRT703446 FYTTD1 forty-two-three domain containing 1 2 2
MIRT704397 CTSS cathepsin S 2 2
MIRT704485 CPT1A carnitine palmitoyltransferase 1A 2 2
MIRT709822 STPG1 sperm tail PG-rich repeat containing 1 2 2
MIRT710859 COQ7 coenzyme Q7, hydroxylase 2 2
MIRT711893 INSIG2 insulin induced gene 2 2 2
MIRT713796 CPLX2 complexin 2 2 2
MIRT718800 C1GALT1C1 C1GALT1 specific chaperone 1 2 2
MIRT719501 SEC24B SEC24 homolog B, COPII coat complex component 2 2
MIRT719722 PDE6B phosphodiesterase 6B 2 2
MIRT722204 URM1 ubiquitin related modifier 1 2 2
MIRT723523 CLPTM1L CLPTM1 like 2 2
MIRT725471 GRAP2 GRB2-related adaptor protein 2 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-3622b-3p Platinum 23939 sensitive tissue
hsa-miR-3622b-3p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3622b-3p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-3622b-3p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide resistant cell line (Bads-200)

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