pre-miRNA Information
pre-miRNA hsa-mir-758   
Genomic Coordinates chr14: 101026020 - 101026107
Synonyms MIRN758, hsa-mir-758, MIR758
Description Homo sapiens miR-758 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-758-5p
Sequence 15| GAUGGUUGACCAGAGAGCACAC |36
Evidence Experimental
Experiments SOLiD
Editing Events in miRNAs
Modification Type Position on miR Chromosome DNA Strand Genomic Position (hg38) List of PMIDs Variant details
A-to-I 16 14 + 101026049 29233923 MiREDiBase
A-to-I 21 14 + 101026054 29233923 MiREDiBase
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs372417522 1 dbSNP
rs748092616 2 dbSNP
rs1270901332 4 dbSNP
rs1364206491 5 dbSNP
rs1483583954 8 dbSNP
rs770931807 10 dbSNP
rs776760808 11 dbSNP
rs1360350664 12 dbSNP
rs759742151 13 dbSNP
rs1002938972 15 dbSNP
rs770107042 17 dbSNP
rs369242818 20 dbSNP
rs1217090272 21 dbSNP
rs763341052 22 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol ZNF415   
Synonyms ZfLp
Description zinc finger protein 415
Transcript NM_001136038   
Other Transcripts NM_001164309 , NM_018355   
Expression
Putative miRNA Targets on ZNF415
3'UTR of ZNF415
(miRNA target sites are highlighted)
>ZNF415|NM_001136038|3'UTR
   1 TATGGCAAGGTCTTCAGTCAAAGTTTAAATCCTGTGAGTCATCAAAGAATTTATATCAGAGAGAAACCATACAAGTATAA
  81 TAAATGTGGCAAGGTTTTCAGTCACAATTCACTCCTACACAGCATCAGAGAATTTCATTCTTGAGAGAATCCTTACGAGT
 161 ACAGCAAACCCTTCATCACAAGTTCAAGCATTCATTGACATCAGAGTCCATGCTAAAGAGAAATCATATACACCTAACTG
 241 TGTGGCAGAGGCTTCATTTAGGTCTCACAACTCACTAGACATCAAAATGTGTAAACATCTTTGTATATTTTGTGCATGTT
 321 GAAGCTATTAACCAAGGATCAAAACTGTAACACATCCAAGGATTTATGTGAGGAATAATTCAGTCTAGTTGTGCTGATAA
 401 ACTTTTCATATTACACATTGTAGAACAAATGCAAGCCCAAATATGTTAAAACTCACACAACATGATATATATTAAAGGTT
 481 GCAGGATGTTTGAAGTCAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' caCACGAGAGAC--C---AGUUGGUag 5'
            |||| : :||  |   |:|||||  
Target 5' ttGTGCATGTTGAAGCTATTAACCAag 3'
310 - 336 118.00 -9.94
2
miRNA  3' cacacgAGAG-ACCAGUUGGUAg 5'
                || |  | |||| ||| 
Target 5' ccttcaTCACAAGTTCAAGCATt 3'
170 - 192 116.00 -5.20
3
miRNA  3' cacacgagagaccagUUGGUAg 5'
                         ||:||| 
Target 5' tccatgctaaagagaAATCATa 3'
207 - 228 104.00 -7.06
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30498858 24 COSMIC
COSN20048525 27 COSMIC
COSN30459121 31 COSMIC
COSN30529873 35 COSMIC
COSN24032882 59 COSMIC
COSN31494133 93 COSMIC
COSN31482865 99 COSMIC
COSN31482899 126 COSMIC
COSN30164035 128 COSMIC
COSN31586543 130 COSMIC
COSN31583529 136 COSMIC
COSN31580896 193 COSMIC
COSN28195316 208 COSMIC
COSN16121334 220 COSMIC
COSN20716360 370 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs772350209 5 dbSNP
rs746089986 9 dbSNP
rs779249429 12 dbSNP
rs757554565 13 dbSNP
rs754268381 16 dbSNP
rs778231370 19 dbSNP
rs770531962 21 dbSNP
rs1181274057 22 dbSNP
rs748509067 23 dbSNP
rs927308527 25 dbSNP
rs756634238 30 dbSNP
rs751028097 31 dbSNP
rs1368658885 32 dbSNP
rs1282166314 36 dbSNP
rs574129828 37 dbSNP
rs368670021 38 dbSNP
rs1359980648 40 dbSNP
rs750039414 47 dbSNP
rs1422084028 48 dbSNP
rs1045993363 54 dbSNP
rs950285690 67 dbSNP
rs1456802056 71 dbSNP
rs1291307974 72 dbSNP
rs913464722 78 dbSNP
rs1387064117 105 dbSNP
rs988954086 107 dbSNP
rs1277870980 108 dbSNP
rs184627767 119 dbSNP
rs1411394037 120 dbSNP
rs868166114 142 dbSNP
rs925029203 149 dbSNP
rs979194798 156 dbSNP
rs1045764 157 dbSNP
rs918888187 163 dbSNP
rs1267304951 169 dbSNP
rs1357135548 170 dbSNP
rs973186208 171 dbSNP
rs1207628647 189 dbSNP
rs1291881476 193 dbSNP
rs1457435325 195 dbSNP
rs1017934038 197 dbSNP
rs1183389649 210 dbSNP
rs1255845728 225 dbSNP
rs1349264998 227 dbSNP
rs192915049 231 dbSNP
rs955286621 232 dbSNP
rs910425862 240 dbSNP
rs115920216 245 dbSNP
rs994409795 247 dbSNP
rs117645919 257 dbSNP
rs1368349210 258 dbSNP
rs1192578084 268 dbSNP
rs1308426809 270 dbSNP
rs1487716083 271 dbSNP
rs1378086354 274 dbSNP
rs1394285320 277 dbSNP
rs954656711 284 dbSNP
rs1017469342 289 dbSNP
rs1341195451 291 dbSNP
rs1227714889 297 dbSNP
rs572593605 312 dbSNP
rs1188985209 322 dbSNP
rs998704486 326 dbSNP
rs1255844413 328 dbSNP
rs1482475358 333 dbSNP
rs1184165837 338 dbSNP
rs967354128 354 dbSNP
rs1022010803 356 dbSNP
rs1187415952 359 dbSNP
rs1011693509 360 dbSNP
rs552841522 367 dbSNP
rs759910921 369 dbSNP
rs905883498 377 dbSNP
rs553381483 381 dbSNP
rs751697383 383 dbSNP
rs1358420811 385 dbSNP
rs1316513801 387 dbSNP
rs534395353 389 dbSNP
rs1257828191 393 dbSNP
rs1219578272 410 dbSNP
rs894546839 415 dbSNP
rs566960133 416 dbSNP
rs1434210409 429 dbSNP
rs1299425080 430 dbSNP
rs3814 443 dbSNP
rs907348095 445 dbSNP
rs1053263305 451 dbSNP
rs1229491078 456 dbSNP
rs936198954 461 dbSNP
rs1300825520 462 dbSNP
rs1310857188 463 dbSNP
rs536970184 465 dbSNP
rs1256906489 474 dbSNP
rs35419779 474 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545216. RNA binding protein: AGO2. Condition:miR-124 transfection ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
CLIP-seq Support 1 for dataset GSM545216
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / miR-124 transfection
Location of target site ENST00000597503.1 | 3UTR | aauucacacuggggagaa
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
62 hsa-miR-758-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT085323 MORC3 MORC family CW-type zinc finger 3 2 2
MIRT089441 STAMBP STAM binding protein 2 2
MIRT089456 TET3 tet methylcytosine dioxygenase 3 2 2
MIRT111856 CCND1 cyclin D1 2 2
MIRT184933 ZNF268 zinc finger protein 268 2 2
MIRT215288 CREBRF CREB3 regulatory factor 2 2
MIRT237300 LPP LIM domain containing preferred translocation partner in lipoma 2 2
MIRT238446 MYO10 myosin X 2 4
MIRT273827 RPL41 ribosomal protein L41 2 2
MIRT282703 HOOK1 hook microtubule tethering protein 1 2 2
MIRT347970 ZNF850 zinc finger protein 850 2 2
MIRT371076 KLF3 Kruppel like factor 3 2 2
MIRT464339 USP6NL USP6 N-terminal like 2 2
MIRT470034 PTP4A1 protein tyrosine phosphatase type IVA, member 1 2 2
MIRT477506 ELL2 elongation factor for RNA polymerase II 2 2 2
MIRT482886 CACNA2D3 calcium voltage-gated channel auxiliary subunit alpha2delta 3 2 2
MIRT492606 POLR3E RNA polymerase III subunit E 2 2
MIRT502294 GNG12 G protein subunit gamma 12 2 6
MIRT507600 DCTN4 dynactin subunit 4 2 4
MIRT510728 SON SON DNA binding protein 2 6
MIRT514065 KCNJ6 potassium voltage-gated channel subfamily J member 6 2 8
MIRT519718 ZNF512B zinc finger protein 512B 2 4
MIRT520890 STRN striatin 2 2
MIRT521760 PPIL1 peptidylprolyl isomerase like 1 2 6
MIRT526874 ERCC8 ERCC excision repair 8, CSA ubiquitin ligase complex subunit 2 2
MIRT530232 WSB2 WD repeat and SOCS box containing 2 2 2
MIRT532003 ACTR2 ARP2 actin related protein 2 homolog 2 2
MIRT533371 UBE2D4 ubiquitin conjugating enzyme E2 D4 (putative) 2 4
MIRT547106 PIGW phosphatidylinositol glycan anchor biosynthesis class W 2 2
MIRT548189 FOXA1 forkhead box A1 2 2
MIRT552935 VKORC1L1 vitamin K epoxide reductase complex subunit 1 like 1 2 2
MIRT560085 ZNF195 zinc finger protein 195 2 2
MIRT561726 PPP2CA protein phosphatase 2 catalytic subunit alpha 2 2
MIRT562713 ZNF415 zinc finger protein 415 2 2
MIRT562761 ZNF846 zinc finger protein 846 2 2
MIRT564159 ZNF117 zinc finger protein 117 2 2
MIRT565673 SETD5 SET domain containing 5 2 2
MIRT565718 SESN3 sestrin 3 2 2
MIRT566026 RFX1 regulatory factor X1 2 2
MIRT569048 ZNF655 zinc finger protein 655 2 2
MIRT570367 UBE2V1 ubiquitin conjugating enzyme E2 V1 2 2
MIRT570410 TMEM189-UBE2V1 TMEM189-UBE2V1 readthrough 2 2
MIRT570443 TMEM189 transmembrane protein 189 2 2
MIRT571738 RNF11 ring finger protein 11 2 2
MIRT575042 Tpgs2 tubulin polyglutamylase complex subunit 2 2 4
MIRT614330 ZDHHC22 zinc finger DHHC-type containing 22 2 2
MIRT617629 RAB3IP RAB3A interacting protein 2 2
MIRT621667 UBE4B ubiquitination factor E4B 2 2
MIRT639906 SRGAP2 SLIT-ROBO Rho GTPase activating protein 2 2 2
MIRT651436 XRCC5 X-ray repair cross complementing 5 2 2
MIRT683853 ZNF208 zinc finger protein 208 2 2
MIRT684841 TPGS2 tubulin polyglutamylase complex subunit 2 2 5
MIRT689347 ZNF83 zinc finger protein 83 2 2
MIRT692492 SPIN4 spindlin family member 4 2 2
MIRT695711 OLA1 Obg like ATPase 1 2 2
MIRT698219 TMEM248 transmembrane protein 248 2 2
MIRT711560 FAM20B FAM20B, glycosaminoglycan xylosylkinase 2 2
MIRT712867 TMEM67 transmembrane protein 67 2 2
MIRT722956 TSPAN1 tetraspanin 1 2 2
MIRT723622 SOBP sine oculis binding protein homolog 2 2
MIRT724176 ABCF2 ATP binding cassette subfamily F member 2 2 2
MIRT755363 LMBR1 limb development membrane protein 1 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-758 Hydroxycamptothecin (HCPT) NULL 97226 Microarray human Tenon's fibroblasts (HTFs) 24681041 2014 down-regulated
miR-758 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 up-regulated
miR-758 5-Fluorouracil approved 3385 Quantitative real-time PCR human squamous cell carcinoma cell line KYSE410 21743970 2011 up-regulated
miR-758 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-758 Fluorouracil 3385 NSC19893 approved resistant cell line (KYSE)
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (HCC827)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Paclitaxel 36314 NSC125973 approved sensitive cell line (BAS)
hsa-miR-758-5p Doxorubicin 31703 NSC123127 approved sensitive cell line (BAS)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-758-5p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-758-5p Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)

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