pre-miRNA Information | |
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pre-miRNA | hsa-mir-3152 |
Genomic Coordinates | chr9: 18573306 - 18573379 |
Description | Homo sapiens miR-3152 stem-loop |
Comment | Berezikov et al. proposed this sequence as a miRNA candidate based on the RAKE method . |
RNA Secondary Structure |
Mature miRNA Information | |||||||||||||||||||||||||
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Mature miRNA | hsa-miR-3152-5p | ||||||||||||||||||||||||
Sequence | 11| AUUGCCUCUGUUCUAACACAAG |32 | ||||||||||||||||||||||||
Evidence | Experimental | ||||||||||||||||||||||||
Experiments | Illumina | ||||||||||||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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miRNAs in Extracellular Vesicles |
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Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | ISCA1 | ||||||||||||||||||||
Synonyms | HBLD2, ISA1, hIscA | ||||||||||||||||||||
Description | iron-sulfur cluster assembly 1 | ||||||||||||||||||||
Transcript | NM_030940 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on ISCA1 | |||||||||||||||||||||
3'UTR of ISCA1 (miRNA target sites are highlighted) |
>ISCA1|NM_030940|3'UTR 1 AATCTCAGGACTCTTCTGGCCGTAGGTTCCAGGAAAGCTCGTGGAAGCTTTGGGGCTCACTGCAGAAATCATGTGACTGT 81 CACGTGCTGGAAAATAAAGTGATACATCTTGAAAATGAATCCAGTGTGTTGGATTCCAGAAGAAATGATATTTATATTCT 161 CTATAGGGGACAGAAAATGAGAAGCCATCACTCTTTTTGGATCATTTAGGTCTCTTGTATCCTTTGTTTTAGAACCAGTT 241 TCATTAAAGTTGCCTTCCTGGGCACCTGTTTATCCATTTCCTGAACTGTGTGCACTCCTTAGATCGCTATTGATGGCTTG 321 ATCATCCCTCAGCATTTCTCCCAACCAGATCGGTGACTCCTAAAATCTGAGACAGGACATCGTGACTGCTGGTAGTAATA 401 TGGTGGTGCATTGTTTTTTCCACCCAAACTTAACATAGCCTTTTTATACATTTTTATGAAAAATTTCATTGTCAGCTGCC 481 TCACTGCATACTCTTTAATAGTACCAGGCAAAGATTTTCTTCAACTATAGTACAGATTAGTTCTGAGTGATGGTATCAAA 561 AGGTGAGAAAGACGTCATCCGCCTTTTTTTAATCCATTTCTTTTGCCACCCTATATGTCTGTTCAGAGATGGGCTCTCAA 641 GCTGACTTTGATTCTTTTAGTTGAGAAGTCTCTTAAAGCCATCTAGCCCACCTCCATCAATTCCCTATGTGAGGAAGCAA 721 AACCCCAGGGAAGCCAAAGGGCTCCTGTCCACCCTGACACCACAGGCCGGGGGAGAGTAGGGACTCTACCCCCCTCTCCC 801 CTTGTAGGTGACACATGCTCTGCCCTCTGAGGCAGTCAGCGAAGGCAAATGGTCTGACTTCTTTATGTGGTCAACATTTT 881 GATAGAATTTCTTTATAATTTGATAGAGATTATATTATTTTTATTTTATTTTGAGTGGGAAGAATTTTAAAACCTTTTTA 961 TGTCAATTACCATCTTGTTTCTTTCACCTTTGAAACAATGGTTTGTAGCAGAGATGACATTGTAGCAACCCAGAATTATG 1041 CTTTTGGAATGTGGTCCTCACTGTACAGGAGAATGTGTAATCTTTTGTTAAAATTCCCAGTGTGCATACATTTTCTGGTT 1121 CCTCGGTCCAGTTGCTAAAGTTCTTAGTATTTTAGCCTAACATATTTATCACCAACTTTTCTTTAAAAGTGTTCCTTTTG 1201 TCACTTAGTTACTGATTTTCCTGGGTTTGACATAAGTATTCTATGAGATGATATATATGCTTTTTTTGAAAGCTGATTCT 1281 CATGAATTCAAGTAGCTGAGTTCCTTTATGTTTCGTTTATTCACTAAAGTAGCTGACACAAAACACACCAAAACCTAGAG 1361 CGGTAGTTTTATGTAAATGCTCATGAGTTTGTATCAATAATATAATTGTTGATCCACTTATAATTCGTGCAACACTGTAT 1441 GTATGTAGAGATTGAGTTGTCAATTAAAAAAAATGTGGCCTCTTTGTGATCATAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | HEK293 | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
PAR-CLIP data was present in GSM545212. RNA binding protein: AGO1. Condition:Control
PAR-CLIP data was present in GSM545216. RNA binding protein: AGO2. Condition:miR-124 transfection
... - Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Hafner M; Landthaler M; Burger L; Khorshid et al. - Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
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CLIP-seq Support 1 for dataset GSM4903832 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Human neurons / 124TD_shELAVL3_b |
Location of target site | NM_030940 | 3UTR | GGCAAAGAUUUUCUUC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161238 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM545212 | |
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Method / RBP | PAR-CLIP / AGO1 |
Cell line / Condition | HEK293 / Control |
Location of target site | ENST00000375991.4 | 3UTR | CAUACUCUUUAAUAGUACCAGGCAAAG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 20371350 / GSE21578 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM545216 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | HEK293 / miR-124 transfection |
Location of target site | ENST00000375991.4 | 3UTR | CAUACUCUUUAAUAGUACCAGGCAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 20371350 / GSE21578 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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75 hsa-miR-3152-5p Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT092535 | KBTBD8 | kelch repeat and BTB domain containing 8 | 2 | 6 | ||||||||
MIRT461731 | NDUFA2 | NADH:ubiquinone oxidoreductase subunit A2 | 2 | 2 | ||||||||
MIRT468456 | SET | SET nuclear proto-oncogene | 2 | 2 | ||||||||
MIRT482192 | AHR | aryl hydrocarbon receptor | 2 | 2 | ||||||||
MIRT507923 | BZW1 | basic leucine zipper and W2 domains 1 | 2 | 4 | ||||||||
MIRT512208 | C18orf25 | chromosome 18 open reading frame 25 | 2 | 6 | ||||||||
MIRT526238 | STARD4 | StAR related lipid transfer domain containing 4 | 2 | 2 | ||||||||
MIRT536131 | MAPK14 | mitogen-activated protein kinase 14 | 2 | 2 | ||||||||
MIRT536280 | LIMA1 | LIM domain and actin binding 1 | 2 | 2 | ||||||||
MIRT544251 | LRIF1 | ligand dependent nuclear receptor interacting factor 1 | 2 | 2 | ||||||||
MIRT548054 | GOLGA7 | golgin A7 | 2 | 2 | ||||||||
MIRT563443 | ISCA1 | iron-sulfur cluster assembly 1 | 2 | 2 | ||||||||
MIRT565643 | SIX4 | SIX homeobox 4 | 2 | 2 | ||||||||
MIRT614870 | PDE12 | phosphodiesterase 12 | 2 | 2 | ||||||||
MIRT614923 | MARCH6 | membrane associated ring-CH-type finger 6 | 2 | 2 | ||||||||
MIRT615793 | FAM124A | family with sequence similarity 124 member A | 2 | 2 | ||||||||
MIRT618557 | VLDLR | very low density lipoprotein receptor | 2 | 2 | ||||||||
MIRT622147 | SORBS2 | sorbin and SH3 domain containing 2 | 2 | 2 | ||||||||
MIRT622390 | RSBN1 | round spermatid basic protein 1 | 2 | 2 | ||||||||
MIRT624185 | DDX19B | DEAD-box helicase 19B | 2 | 2 | ||||||||
MIRT624448 | CALCOCO2 | calcium binding and coiled-coil domain 2 | 2 | 2 | ||||||||
MIRT625340 | TAPBP | TAP binding protein | 2 | 2 | ||||||||
MIRT625370 | IRGQ | immunity related GTPase Q | 2 | 2 | ||||||||
MIRT627276 | WSB1 | WD repeat and SOCS box containing 1 | 2 | 2 | ||||||||
MIRT627746 | RAD54L2 | RAD54 like 2 | 2 | 2 | ||||||||
MIRT629578 | RFC2 | replication factor C subunit 2 | 2 | 4 | ||||||||
MIRT630537 | LGALS8 | galectin 8 | 2 | 4 | ||||||||
MIRT630559 | C3orf36 | chromosome 3 open reading frame 36 | 2 | 4 | ||||||||
MIRT630568 | SOWAHA | sosondowah ankyrin repeat domain family member A | 2 | 4 | ||||||||
MIRT630763 | MSANTD3 | Myb/SANT DNA binding domain containing 3 | 2 | 2 | ||||||||
MIRT635424 | LRP10 | LDL receptor related protein 10 | 2 | 2 | ||||||||
MIRT636011 | GNPNAT1 | glucosamine-phosphate N-acetyltransferase 1 | 2 | 2 | ||||||||
MIRT636337 | PHAX | phosphorylated adaptor for RNA export | 2 | 2 | ||||||||
MIRT638413 | POU3F2 | POU class 3 homeobox 2 | 2 | 2 | ||||||||
MIRT639358 | INIP | INTS3 and NABP interacting protein | 2 | 2 | ||||||||
MIRT642124 | DYRK1A | dual specificity tyrosine phosphorylation regulated kinase 1A | 2 | 4 | ||||||||
MIRT642828 | KIAA0319 | KIAA0319 | 2 | 2 | ||||||||
MIRT644017 | NUCB1 | nucleobindin 1 | 2 | 2 | ||||||||
MIRT644038 | WWC2 | WW and C2 domain containing 2 | 2 | 2 | ||||||||
MIRT644153 | GIN1 | gypsy retrotransposon integrase 1 | 2 | 2 | ||||||||
MIRT644649 | SNX9 | sorting nexin 9 | 2 | 2 | ||||||||
MIRT645798 | OMA1 | OMA1 zinc metallopeptidase | 2 | 2 | ||||||||
MIRT647633 | FAIM2 | Fas apoptotic inhibitory molecule 2 | 2 | 2 | ||||||||
MIRT648528 | KIAA1143 | KIAA1143 | 2 | 2 | ||||||||
MIRT648851 | PDLIM3 | PDZ and LIM domain 3 | 2 | 2 | ||||||||
MIRT648880 | TUBGCP5 | tubulin gamma complex associated protein 5 | 2 | 2 | ||||||||
MIRT650777 | TNFRSF10A | TNF receptor superfamily member 10a | 2 | 2 | ||||||||
MIRT654200 | RNF19B | ring finger protein 19B | 2 | 2 | ||||||||
MIRT654508 | RAB5B | RAB5B, member RAS oncogene family | 2 | 2 | ||||||||
MIRT655249 | PEX26 | peroxisomal biogenesis factor 26 | 2 | 2 | ||||||||
MIRT656089 | MTA3 | metastasis associated 1 family member 3 | 2 | 2 | ||||||||
MIRT656599 | LRRC55 | leucine rich repeat containing 55 | 2 | 2 | ||||||||
MIRT659194 | CYBB | cytochrome b-245 beta chain | 2 | 2 | ||||||||
MIRT660000 | C1orf115 | chromosome 1 open reading frame 115 | 2 | 4 | ||||||||
MIRT661715 | KLF8 | Kruppel like factor 8 | 2 | 2 | ||||||||
MIRT661813 | PRPSAP1 | phosphoribosyl pyrophosphate synthetase associated protein 1 | 2 | 2 | ||||||||
MIRT668360 | FGD4 | FYVE, RhoGEF and PH domain containing 4 | 2 | 2 | ||||||||
MIRT679734 | CABP4 | calcium binding protein 4 | 2 | 2 | ||||||||
MIRT708616 | NUDT18 | nudix hydrolase 18 | 2 | 2 | ||||||||
MIRT709034 | TROVE2 | TROVE domain family member 2 | 2 | 2 | ||||||||
MIRT709573 | DMGDH | dimethylglycine dehydrogenase | 2 | 2 | ||||||||
MIRT709665 | AJAP1 | adherens junctions associated protein 1 | 2 | 2 | ||||||||
MIRT709874 | TRAF1 | TNF receptor associated factor 1 | 2 | 2 | ||||||||
MIRT710821 | NLRP12 | NLR family pyrin domain containing 12 | 2 | 2 | ||||||||
MIRT710883 | PARL | presenilin associated rhomboid like | 2 | 2 | ||||||||
MIRT711547 | LCAT | lecithin-cholesterol acyltransferase | 2 | 2 | ||||||||
MIRT712017 | STX1B | syntaxin 1B | 2 | 2 | ||||||||
MIRT713058 | BLVRA | biliverdin reductase A | 2 | 2 | ||||||||
MIRT713129 | THRB | thyroid hormone receptor beta | 2 | 2 | ||||||||
MIRT713878 | MOB3A | MOB kinase activator 3A | 2 | 2 | ||||||||
MIRT714154 | NXPH3 | neurexophilin 3 | 2 | 2 | ||||||||
MIRT719755 | FLYWCH1 | FLYWCH-type zinc finger 1 | 2 | 2 | ||||||||
MIRT720213 | KCNK1 | potassium two pore domain channel subfamily K member 1 | 2 | 2 | ||||||||
MIRT722728 | BRMS1 | breast cancer metastasis suppressor 1 | 2 | 2 | ||||||||
MIRT725652 | ABI2 | abl interactor 2 | 2 | 2 |