pre-miRNA Information
pre-miRNA hsa-mir-4291   
Genomic Coordinates chr9: 93819357 - 93819421
Description Homo sapiens miR-4291 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4291
Sequence 11| UUCAGCAGGAACAGCU |26
Evidence Experimental
Experiments SOLiD
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1265713434 9 dbSNP
rs1433969451 12 dbSNP
rs1372852268 16 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol POLQ   
Synonyms PRO0327
Description DNA polymerase theta
Transcript NM_199420   
Expression
Putative miRNA Targets on POLQ
3'UTR of POLQ
(miRNA target sites are highlighted)
>POLQ|NM_199420|3'UTR
   1 CTGTGCTGTTGATGAAGTCCTCCCAGGGAAGCCTGTGCAGATGCAGTCACCTGGAAAGAACAGAGATTACCCTTTCACCT
  81 ACCTCAGCAAAACAAACTTTCAAGTCTTGATAGACTTAGCCTAGTAATTTTATAGTGAGAGTTTCAAACTATATATCAGT
 161 GTCTATAGCATCAAAAACTTCTGGGGGCGTGGGGGAAGTAGAATACCAAGTATAATAGTTACATTCACTTTCAAAGAGCA
 241 TCTATGAATTTGCCTTTTGTAACTTACTGTGGCTTTAAACATATTCAGAACAGATGCTTGAAATATGCACTTAGCACTTT
 321 GGTTCCACATCTGTCTGGGTAAACCATGAAGAAAATGAAGCTGCTGCCTCAATCGACCCAGACAGCAGCCATAGGCAGAT
 401 AAAGATTTGGTTTCACCCTGGTGGTGGTAGGCATCGTGTGTGACTTTTTTTCCTCTAATATCAATTTTACAGTACGGAAA
 481 TAGTATTTTAAAATAGTATTGGCTAATAAATTATGAATTCTATAAAGTAGTAAGACTTGGTATGGTTGGAGTGTAGGAAT
 561 GAATATTCATGAAATGTTTCTTATTGCTTTTCCTTCCCTAATTCATACAATGAATGTATTTGGAATACTTACATATTATA
 641 AAATAAACTATACCTCTTCAAGAGGTATCCTGTTCTGTAAGATCAGATGTTTTTATTGCAGGTCAATATAATACTGCCAG
 721 AGACAGAAAATACCCCCTTATCAGTCCCTTAGTGCCTCTTTCTGTTTGTGGCATGGTGAGAAAACCCATGCTGAAAAGAT
 801 TGTACTTTGTGATCCCAATCAGAGGGATGGAGCTAATCTTTTTGCTGTTGAAATAAAATGAATTTATGAGAAACTTTAAA
 881 AAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' ucGACAAGGACGACUu 5'
            :| || |||:||| 
Target 5' tcTTTTTGCTGTTGAa 3'
837 - 852 134.00 -9.40
2
miRNA  3' ucGACAAGGACGACUu 5'
            ||| | |||:||| 
Target 5' --CTG-TGCTGTTGAt 3'
1 - 13 133.00 -11.40
3
miRNA  3' ucgACAAGGACGACuu 5'
             ||   ||||||  
Target 5' aaaTGAAGCTGCTGcc 3'
353 - 368 121.00 -8.30
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30155667 12 COSMIC
COSN30481982 19 COSMIC
COSN30509443 24 COSMIC
COSN30179949 34 COSMIC
COSN31594287 46 COSMIC
COSN31574799 54 COSMIC
COSN30607544 88 COSMIC
COSN30164166 159 COSMIC
COSN20811685 205 COSMIC
COSN32062611 347 COSMIC
COSN26646791 452 COSMIC
COSN16442080 869 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs372121874 3 dbSNP
rs890792986 13 dbSNP
rs1443187831 17 dbSNP
rs766451729 18 dbSNP
rs756270330 19 dbSNP
rs201375138 23 dbSNP
rs1418512783 24 dbSNP
rs1260597637 25 dbSNP
rs763908065 33 dbSNP
rs574058342 34 dbSNP
rs1290531671 36 dbSNP
rs189468665 38 dbSNP
rs765321041 39 dbSNP
rs774783608 39 dbSNP
rs1177515087 41 dbSNP
rs1481415620 43 dbSNP
rs1235938815 44 dbSNP
rs1377950691 61 dbSNP
rs543594058 71 dbSNP
rs925064562 96 dbSNP
rs1399477628 97 dbSNP
rs1298922507 101 dbSNP
rs114750988 121 dbSNP
rs1468185657 139 dbSNP
rs1385542657 141 dbSNP
rs947577239 148 dbSNP
rs558035695 151 dbSNP
rs1031060213 153 dbSNP
rs1199265249 154 dbSNP
rs1235906352 158 dbSNP
rs1427655728 159 dbSNP
rs534936465 165 dbSNP
rs960354234 168 dbSNP
rs928624366 183 dbSNP
rs980123775 188 dbSNP
rs970117641 189 dbSNP
rs185567338 194 dbSNP
rs768346337 203 dbSNP
rs1434676853 204 dbSNP
rs1198286591 207 dbSNP
rs1030244596 211 dbSNP
rs760252616 217 dbSNP
rs199746530 222 dbSNP
rs73855391 223 dbSNP
rs1234644481 227 dbSNP
rs1419379800 228 dbSNP
rs1159491754 238 dbSNP
rs961504964 239 dbSNP
rs775086410 244 dbSNP
rs1388882962 246 dbSNP
rs1295882418 259 dbSNP
rs1437467265 263 dbSNP
rs1376659383 267 dbSNP
rs1240370778 268 dbSNP
rs1008551792 283 dbSNP
rs1319346682 284 dbSNP
rs1220266418 287 dbSNP
rs1265960575 293 dbSNP
rs1489448715 304 dbSNP
rs535130027 306 dbSNP
rs1237285663 309 dbSNP
rs1440110021 310 dbSNP
rs1039819039 311 dbSNP
rs771732671 313 dbSNP
rs749775954 315 dbSNP
rs1422116638 316 dbSNP
rs1412874653 322 dbSNP
rs1049373177 333 dbSNP
rs1404474971 344 dbSNP
rs1047531288 347 dbSNP
rs1459854888 348 dbSNP
rs569726663 357 dbSNP
rs145816626 360 dbSNP
rs929981418 366 dbSNP
rs778394643 367 dbSNP
rs903590042 372 dbSNP
rs537204476 374 dbSNP
rs571530580 375 dbSNP
rs1312865104 381 dbSNP
rs1426119320 385 dbSNP
rs947546006 387 dbSNP
rs1216678315 388 dbSNP
rs891952552 432 dbSNP
rs140696912 433 dbSNP
rs116685716 435 dbSNP
rs958049068 436 dbSNP
rs980070066 437 dbSNP
rs1184740897 459 dbSNP
rs923699878 464 dbSNP
rs1169093722 471 dbSNP
rs978194942 473 dbSNP
rs747799936 475 dbSNP
rs780762832 476 dbSNP
rs1030255842 477 dbSNP
rs996060684 483 dbSNP
rs919968222 502 dbSNP
rs1446766103 508 dbSNP
rs559648878 527 dbSNP
rs974161028 528 dbSNP
rs1380620606 536 dbSNP
rs1397600758 540 dbSNP
rs1318601510 542 dbSNP
rs1342083599 542 dbSNP
rs961517539 543 dbSNP
rs1015839426 550 dbSNP
rs1311423537 552 dbSNP
rs1209600547 553 dbSNP
rs1272934015 555 dbSNP
rs549356217 562 dbSNP
rs146880375 568 dbSNP
rs955443258 581 dbSNP
rs1283773151 619 dbSNP
rs1016082105 629 dbSNP
rs1342280515 630 dbSNP
rs1423447974 635 dbSNP
rs1334929423 639 dbSNP
rs1414262544 641 dbSNP
rs1176257424 653 dbSNP
rs149864930 666 dbSNP
rs996431627 668 dbSNP
rs1297086960 669 dbSNP
rs1341026798 677 dbSNP
rs1401724725 685 dbSNP
rs748533553 686 dbSNP
rs11555758 691 dbSNP
rs1366574807 695 dbSNP
rs1219649311 696 dbSNP
rs1279599689 698 dbSNP
rs1214516912 708 dbSNP
rs1170225345 711 dbSNP
rs1021951537 719 dbSNP
rs1025721959 730 dbSNP
rs139458640 736 dbSNP
rs1477895891 740 dbSNP
rs1192203773 747 dbSNP
rs1384413221 752 dbSNP
rs1417534576 755 dbSNP
rs1161047059 757 dbSNP
rs1360533388 758 dbSNP
rs1473583333 761 dbSNP
rs1368475505 764 dbSNP
rs906507194 788 dbSNP
rs1046641361 800 dbSNP
rs948281685 802 dbSNP
rs1180557273 826 dbSNP
rs892239471 827 dbSNP
rs895381694 833 dbSNP
rs1053684974 838 dbSNP
rs751801003 847 dbSNP
rs564288350 861 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545214. RNA binding protein: AGO3. Condition:Control ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' ucgacaagGACGACUu 5'
                  ||||||| 
Target 5' -aaucccaCUGCUGAc 3'
1 - 15
Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
CLIP-seq Support 1 for dataset GSM545214
Method / RBP PAR-CLIP / AGO3
Cell line / Condition HEK293 / Control
Location of target site ENST00000372226.1 | 3UTR | AAUCCCACUGCUGACAGA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
82 hsa-miR-4291 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT102445 CALU calumenin 2 4
MIRT108677 ZBTB33 zinc finger and BTB domain containing 33 2 4
MIRT125969 SHOC2 SHOC2, leucine rich repeat scaffold protein 2 6
MIRT179018 PAFAH1B2 platelet activating factor acetylhydrolase 1b catalytic subunit 2 2 4
MIRT379033 CDK6 cyclin dependent kinase 6 2 6
MIRT442473 CPEB4 cytoplasmic polyadenylation element binding protein 4 2 2
MIRT442910 PCBD2 pterin-4 alpha-carbinolamine dehydratase 2 2 2
MIRT442979 ZNF736 zinc finger protein 736 2 2
MIRT445663 TNFSF15 TNF superfamily member 15 2 2
MIRT446237 FZD6 frizzled class receptor 6 2 2
MIRT448860 FAM49B family with sequence similarity 49 member B 2 2
MIRT455559 TRAF1 TNF receptor associated factor 1 2 2
MIRT459704 ZNF641 zinc finger protein 641 2 2
MIRT460608 FEM1A fem-1 homolog A 2 2
MIRT462251 LAMA4 laminin subunit alpha 4 2 2
MIRT462469 FIZ1 FLT3 interacting zinc finger 1 2 2
MIRT466656 TAF1D TATA-box binding protein associated factor, RNA polymerase I subunit D 2 6
MIRT466841 STX6 syntaxin 6 2 2
MIRT471403 PDP2 pyruvate dehyrogenase phosphatase catalytic subunit 2 2 2
MIRT471517 PCGF3 polycomb group ring finger 3 2 2
MIRT471681 PABPN1 poly(A) binding protein nuclear 1 2 2
MIRT472858 MTHFD2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase 2 2
MIRT474813 KIAA0226 RUN and cysteine rich domain containing beclin 1 interacting protein 2 2
MIRT474931 KCTD15 potassium channel tetramerization domain containing 15 2 2
MIRT475814 HDGF heparin binding growth factor 2 2
MIRT480434 C17orf49 chromosome 17 open reading frame 49 2 2
MIRT480903 BCL2L2-PABPN1 BCL2L2-PABPN1 readthrough 2 2
MIRT481478 ARL8B ADP ribosylation factor like GTPase 8B 2 2
MIRT484982 UBE2V1 ubiquitin conjugating enzyme E2 V1 2 8
MIRT485019 TMEM189-UBE2V1 TMEM189-UBE2V1 readthrough 2 8
MIRT485036 TMEM189 transmembrane protein 189 2 8
MIRT495074 HEYL hes related family bHLH transcription factor with YRPW motif-like 2 2
MIRT496004 CD180 CD180 molecule 2 2
MIRT500675 TRIM37 tripartite motif containing 37 2 2
MIRT504544 ZNF417 zinc finger protein 417 2 6
MIRT506782 KLHL15 kelch like family member 15 2 6
MIRT507256 FGF2 fibroblast growth factor 2 2 6
MIRT507386 EN2 engrailed homeobox 2 2 2
MIRT512505 BTBD19 BTB domain containing 19 2 2
MIRT516903 CTSB cathepsin B 2 2
MIRT528124 PPP1R10 protein phosphatase 1 regulatory subunit 10 2 2
MIRT528874 ATF3 activating transcription factor 3 2 2
MIRT536091 MBOAT2 membrane bound O-acyltransferase domain containing 2 2 2
MIRT541079 RLIM ring finger protein, LIM domain interacting 2 2
MIRT541099 RAF1 Raf-1 proto-oncogene, serine/threonine kinase 2 2
MIRT545360 LIN7C lin-7 homolog C, crumbs cell polarity complex component 2 2
MIRT545831 ZNF367 zinc finger protein 367 2 4
MIRT547115 PHLPP2 PH domain and leucine rich repeat protein phosphatase 2 2 2
MIRT547312 NPTN neuroplastin 2 2
MIRT547959 HIGD1A HIG1 hypoxia inducible domain family member 1A 2 4
MIRT549943 RPL7L1 ribosomal protein L7 like 1 2 2
MIRT550749 ENTPD1 ectonucleoside triphosphate diphosphohydrolase 1 2 2
MIRT565145 TUBB2A tubulin beta 2A class IIa 2 2
MIRT571050 POLQ DNA polymerase theta 2 2
MIRT571361 ZNF45 zinc finger protein 45 2 2
MIRT610133 FOXI2 forkhead box I2 2 2
MIRT613145 DSE dermatan sulfate epimerase 2 2
MIRT613379 ABCC12 ATP binding cassette subfamily C member 12 2 2
MIRT615752 C6 complement C6 2 2
MIRT616460 ADRA2B adrenoceptor alpha 2B 2 2
MIRT616719 FEM1B fem-1 homolog B 2 2
MIRT618312 IPP intracisternal A particle-promoted polypeptide 2 2
MIRT631491 RASSF4 Ras association domain family member 4 2 2
MIRT643134 PLCXD2 phosphatidylinositol specific phospholipase C X domain containing 2 2 2
MIRT643482 DISC1 disrupted in schizophrenia 1 2 2
MIRT649715 TWSG1 twisted gastrulation BMP signaling modulator 1 2 2
MIRT653542 SLC38A7 solute carrier family 38 member 7 2 2
MIRT666307 SLC22A3 solute carrier family 22 member 3 2 2
MIRT692005 NAP1L4 nucleosome assembly protein 1 like 4 2 2
MIRT696838 ARL2BP ADP ribosylation factor like GTPase 2 binding protein 2 2
MIRT698204 TMEM248 transmembrane protein 248 2 2
MIRT703316 GDPD5 glycerophosphodiester phosphodiesterase domain containing 5 2 2
MIRT709376 FAM13A family with sequence similarity 13 member A 2 2
MIRT710112 MED23 mediator complex subunit 23 2 2
MIRT711975 HOMER2 homer scaffolding protein 2 2 2
MIRT712275 PPIP5K2 diphosphoinositol pentakisphosphate kinase 2 2 2
MIRT714108 TMED9 transmembrane p24 trafficking protein 9 2 2
MIRT719113 MAML1 mastermind like transcriptional coactivator 1 2 2
MIRT719727 SLC39A11 solute carrier family 39 member 11 2 2
MIRT720018 TFAP2C transcription factor AP-2 gamma 2 2
MIRT720358 ZBTB8B zinc finger and BTB domain containing 8B 2 2
MIRT720372 NUDT3 nudix hydrolase 3 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4291 Platinum-based doublet chemotherapy resistant High Lung Adenocarcinoma tissue
hsa-miR-4291 Platinum 23939 resistant High Ovarian Cancer tissue
hsa-miR-4291 Paclitaxel 36314 NSC125973 approved resistant High Prostate Cancer cell line (DU-145, PC-3)
hsa-miR-4291 Doxorubicin 31703 NSC123127 approved resistant High Chronic Myelogenous Leukemia cell line (K562)
hsa-miR-4291 Radioactivity Iodine resistant High Papillary Thyroid Cancer tissue
hsa-miR-4291 Cisplatin 5460033 NSC119875 approved resistant High Hypopharyngeal Cancer cell line (FaDu)
hsa-miR-4291 Gefitinib 123631 NSC715055 approved resistant cell line (PC9)
hsa-miR-4291 Osimertinib 71496458 NSC779217 approved resistant cell line (PC9)
hsa-miR-4291 Cisplatin 5460033 NSC119875 approved sensitive cell line (CAL-27) (cytosolic RNA)
hsa-miR-4291 Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)
hsa-miR-4291 Platinum-based doublet chemotherapy resistant tissue (lung adenocarcinoma)
hsa-miR-4291 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-4291 Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR3)
hsa-miR-4291 Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR4)

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