miRTarBase - #MIRT571536


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pre-miRNA Information
pre-miRNA hsa-mir-2117   
Genomic Coordinates chr17: 43444806 - 43444885
Description Homo sapiens miR-2117 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-2117
Sequence 51| UGUUCUCUUUGCCAAGGACAG |71
Evidence Experimental
Experiments 454
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1255384593 12 dbSNP
rs1227862494 14 dbSNP
rs1458444919 16 dbSNP
rs1309101018 17 dbSNP
rs1159219853 18 dbSNP
rs1344029538 19 dbSNP
rs747666550 20 dbSNP
rs770495894 21 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol ZNF286B   
Synonyms ZNF286C, ZNF286L, ZNF590
Description zinc finger protein 286B
Transcript NM_001145045   
Expression
Putative miRNA Targets on ZNF286B
3'UTR of ZNF286B
(miRNA target sites are highlighted)		 
>ZNF286B|NM_001145045|3'UTR
   1 AAAATTACTGAATGTGAAGAAATGTAAGTTGGTTTTATCACTATATTAAATACTTGAGTGTTTAGGTGGAAGGCCCATCC
  81 ATAATATGCATGTGAGGACCAATTCTATATGCATCTAATTTCATTTGTGTAATACATAGTTTTGAGCCATAGGCAGGTTC
 161 TTTATGTCCGTTAATTTGATAATTATGAAATCTAGCCAGCGATTTCAAAATTTTAATCTCCAACCTCCCAAATAGCTATC
 241 TGTGGAAATTCAAGAAGTCCCTGAGAGTGGGTAGCAGTACAAACATTGTGAAATCCAGTTTTCCCCTCTCTTGTTTATGT
 321 CAGAGCTTTGTTGTAAGCCTCTCACTTTGTAAGGAAATTCTTATTGGGTAGGTTAGGCTGAGGGCTTACATCCTTATCAT
 401 CAGGAAGACACAATGTTGTTATTTATTACTCCTTGAGATCTAGATACACATATGGTATAATTCATCTAGAGTGTCATTTC
 481 TTTTATATTAGCCTTAAAAACCAAAAATGCTGGGTTCAAAGTCCTTAGCATTCCCTTCCTCCCTCAACAAGCTGCTGTCT
 561 ATTTTGATGACCATAACTAGGATGTATAATATTTACAGACAATTCTAATTTATGTTCACATGGATTATGTACATCATTGA
 641 TTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCGTGATCTCAGCTCACT
 721 GCAACCTCCACCTCCCAGGTTCATGCCATTCTCTTGTCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACCTGCCAGTAC
 801 GCCTGGCTAATTTTTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTAGCCAGGATGGTCTTGATCTCCTGACCTC
 881 GTGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCATGCCTGGCATCATCATTGATTTATG
 961 TCATTGTTATCCCTCTACCGCGGAGACCTTCTTTCATTTTCCATTTCCCTGGGAGTTCACGTGAAGTAGTGAATGGGTCA
1041 AAATCTTAACAGTGGTTGTTGATCAAGTCCTTCCTTTCTTATCCTTGGCACTGAATAAATCTTTGGTTTTCCATAATCAA
1121 AGGGTGAGGGAGACTTCTTTTGTCTACATTATTCAGCTTTCTGAAAGCTATGCTGTGTCCATTTGAGAAAAAGCCCCAGG
1201 GATATTGAAGGTCTGAGAAGTTGAGTGGTTCAGTGTGTTATTATATTTAGGGTCCCAGTAAAGAAGAACTGAAGCAACTG
1281 TGAGACACCAGGGCATACTTCCCTGTATGAGTGGAAAACAAGGTACGGAAAATAGCTCACAGGATAGTCCTCAACTGACT
1361 TGCTTGAGGATAAGCAGCTTCCCTTCATGAAGAAGTTGCCATTTCTCTGGCCTTCATATTTACCTACTTTTTGTTATTGT
1441 TATCTGTATCGGTTCAATCTCACACTGCTATAAAGAAATGCCTGAGACTGGGTAACTTATAAAGAAAAAAGGTTTAATTG
1521 GCTCATGGTTCCATGGGCTGTACAGGAAGTATGACTGGGGAGGTCTCAGGGAGCTTTTACTCATGGCAAAAGGCAAAGCC
1601 AGAGCAGGTATCTTCACAGGGCTAGAGCAAGAGGAAAAGAGAGAGGGGAGGTGCTACACACTTTTTATGCAACCAAATCT
1681 CATGAGAACTCTATCACGAGACAGCAGTAGGGGGACAGTGCTAAATCATTCATGAGAAACTACCTGCGTGGTCCAGTCAC
1761 CTTGCACCAGGCCCCACCTCCCACATTGGGGATTACAATTAAACATGAGATTTGGGTGGGGATACAGATCTAAACTGTAT
1841 CATTACTCAAAAAAAAAAAAAAAAGAAAAAAGAAAGAAAACACAGGAAAAGGAACTCACCTCTTTTATCATCTTAGTATT
1921 TAGTACTAATGGTTTAGAGCAGGAGTTGACAAACTGTGGCCTGGACTCATGGACCAACTCTGGCCCACCACCTGTTTCTC
2001 AGAAGAAAATTTTATTGGAACTCAGCCACACTTACTCATTTACATGTTGTCCTTGACTGCCTTTGTGTCCAGTGTCAGAA
2081 CTGAGTAGCTGCAGCGGAGGTTGTGCCTCTGGGCTTTAATATCACAAACAGAGCAGCTTATAAGCAACAGAAACTTACTT
2161 CTCACAGTTTTGGAGGCTGGGAAGTCCAAGATCAAGGTGTCAACATATTTAGGGTCTAGGGAAGGTCTGCTTCTTGGTTA
2241 ATAGAAAGCACCTTTCACTGTGTCTTCACATGGTAGAAGGGGAGAGCTCTGGTATCTTCAGGCCCTTATAAGGGCACTAA
2321 TCCCATTCATGAGCGCTCCACCCTCACGACCTAATCACCTCCAAAAGGCCCCATTTCATAATACCATCAATTAAGTTTCA
2401 TTATATGACTTTGATGGGGATATAGCCCATAGTAGGTTCTATGTCCCTGAAAGCCTAAAATATTTACTGTTTGGCTCTTT
2481 ACAGAAAATATTTTCTTGGCCCCTGTTCCAGAGCATTCATCAAGAAGTCAGATAAAGGGTTTAAACCAGTCAGAGAAAGT
2561 AGGTGGAATGGAAAAGTTGAACTTACTGAGAGATAACTGGGGACAGAAAAATAAAAGAGGTGGGAAATGATGCATCAAAA
2641 TACTCCTACCTCTAGGATGTTACTATATATTGTCAGGAGAATTCAAGTTATTGAAACTTGCCAAATTCTTGAGTGAAAAG
2721 AAATTCATTTGAGGGCCAAAATTTGACTAAGGAAAGAAGACCAACATGGGCTTTCCAAAGTTGAGTTTGAATTCTGCCAT
2801 CCCCACTAAGCAGTCATGTGACCTTGGAAAAACTCCCTACTCTGAGTCTCAGTTTCTTTATCTGTATTAGGTTTCAACAA
2881 AGGGAATAAATCATTGGCTTCTCATGAGGTTGTCATAAGGATTAAATAAGACAATGCATACAAAAGAACTGGCAGACAAT
2961 AAATATAGAGTCCCCCACTCCTGCTTGGTGCTCTCTTTCTCTCTCCTTCTCTACATAGAGAAGTGCCAGAGCATCCGTCA
3041 GTAGAAGTTACTTAAGGTTGGCTGTGTCTTCCACATCTTTTGTTTACCCATGAAGAACTGTGACCATCTTGTTCTGTGGC
3121 TTGTCATAAAGATGGGACGTGACTTCTTTCTGCTCTTGCTTCCATCTGGTGCTGTTTTCTGCACAGCCTCCTTGAGACCT
3201 TCCAGAACCTTCTTTTTTCCTACTTCGTATCACTTAACCAGTAGCTATCTGGGATGGTTCCAGAGGCTGAAGTTTGCAGT
3281 TGAATAACATTTTAAATAGTGTTAAGCGACTTGGGATCTCTGGCCCTTTGCGTTTCAGTATTTGTCCTTTTTATGCCTGA
3361 TGTTTAACCTGCCACATGTTTGTACTGCAACATGAATCTTGGAAATTTTAATGTTATGCATTTCAAATGTTTTTGGTTAT
3441 CTGTAAACTAAATGTGCCCTTATTGGCTCACTTGTCAATAAAGATGTTTGTATATGTTTTGTCAGAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gacAGGAACCGUUUCUCUUgu 5'
             |:||   || ||||||  
Target 5' tccTTCTCTACATAGAGAAgt 3'
 3004 - 3024 134.00 -12.70
2 
miRNA  3' gaCAGGAACCGUUUC-UCUUGu 5'
            ||||   |:|||| ||||| 
Target 5' ggGTCC-CAGTAAAGAAGAACt 3'
 1250 - 1270 130.00 -14.90
3 
miRNA  3' gaCAGGAACC------GUUUCUCUUGu 5'
            || :| ||      |||||:|||: 
Target 5' ctGTATTAGGTTTCAACAAAGGGAATa 3'
 2862 - 2888 125.00 -14.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545214. RNA binding protein: AGO3. Condition:Control ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' gacaGGAACCGUUUCUCuugu 5'
              :| |  |::||||    
Target 5' --acUCAUACCGGAGAG---- 3'
 		1 - 15
Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
CLIP-seq Support 1 for dataset GSM545214
Method / RBP PAR-CLIP / AGO3
Cell line / Condition HEK293 / Control
Location of target site ENST00000285274.5 | 3UTR | acucauaccggagag
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
50 hsa-miR-2117 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT080059 TGIF1 TGFB induced factor homeobox 1 2 2
MIRT253245 ZNF264 zinc finger protein 264 2 4
MIRT279706 EIF2S1 eukaryotic translation initiation factor 2 subunit alpha 2 4
MIRT359344 TMEM167A transmembrane protein 167A 2 2
MIRT465598 TNRC6A trinucleotide repeat containing 6A 2 2
MIRT479860 CCDC6 coiled-coil domain containing 6 2 2
MIRT512413 KIAA0391 KIAA0391 2 2
MIRT525342 TUBGCP4 tubulin gamma complex associated protein 4 2 2
MIRT528092 UCHL3 ubiquitin C-terminal hydrolase L3 2 2
MIRT529254 TRIM4 tripartite motif containing 4 2 4
MIRT550782 VAV3 vav guanine nucleotide exchange factor 3 2 2
MIRT554467 SAMD12 sterile alpha motif domain containing 12 2 2
MIRT560164 ZNF286A zinc finger protein 286A 2 2
MIRT562613 BTF3L4 basic transcription factor 3 like 4 2 2
MIRT568507 ARF1 ADP ribosylation factor 1 2 2
MIRT571536 ZNF286B zinc finger protein 286B 2 2
MIRT609660 ITIH5 inter-alpha-trypsin inhibitor heavy chain family member 5 2 2
MIRT612022 PAK6 p21 (RAC1) activated kinase 6 2 8
MIRT615677 NAV2 neuron navigator 2 2 2
MIRT616035 SCO1 SCO1, cytochrome c oxidase assembly protein 2 2
MIRT617771 C17orf105 chromosome 17 open reading frame 105 2 2
MIRT628757 MX2 MX dynamin like GTPase 2 2 4
MIRT635089 AKIRIN1 akirin 1 2 2
MIRT638261 SIX1 SIX homeobox 1 2 2
MIRT638492 NNT nicotinamide nucleotide transhydrogenase 2 2
MIRT639400 NEURL1B neuralized E3 ubiquitin protein ligase 1B 2 2
MIRT641791 USP32 ubiquitin specific peptidase 32 2 2
MIRT642309 FPR1 formyl peptide receptor 1 2 2
MIRT645902 LRIF1 ligand dependent nuclear receptor interacting factor 1 2 2
MIRT649701 ZNF175 zinc finger protein 175 2 2
MIRT656848 KLF13 Kruppel like factor 13 2 2
MIRT659911 CACNG2 calcium voltage-gated channel auxiliary subunit gamma 2 2 2
MIRT667275 NAV1 neuron navigator 1 2 2
MIRT674145 ZNF793 zinc finger protein 793 2 2
MIRT692839 C1orf50 chromosome 1 open reading frame 50 2 2
MIRT698520 TFRC transferrin receptor 2 2
MIRT698731 STX6 syntaxin 6 2 4
MIRT700931 PDPK1 3-phosphoinositide dependent protein kinase 1 2 2
MIRT706080 HNRNPU heterogeneous nuclear ribonucleoprotein U 2 2
MIRT710008 SH3GLB1 SH3 domain containing GRB2 like, endophilin B1 2 2
MIRT711059 UGT2B4 UDP glucuronosyltransferase family 2 member B4 2 2
MIRT711285 PSME3 proteasome activator subunit 3 2 2
MIRT716172 FAM71F2 family with sequence similarity 71 member F2 2 2
MIRT716761 TRABD2A TraB domain containing 2A 2 2
MIRT718683 FZD2 frizzled class receptor 2 2 2
MIRT719675 SPDYE1 speedy/RINGO cell cycle regulator family member E1 2 2
MIRT721610 PPP1CB protein phosphatase 1 catalytic subunit beta 2 2
MIRT722188 DNAJC9 DnaJ heat shock protein family (Hsp40) member C9 2 2
MIRT725156 SEC62 SEC62 homolog, preprotein translocation factor 2 2
MIRT725239 PDE1B phosphodiesterase 1B 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-2117 Ascorbate approved 54670067 Microarray Metastatic melanoma cell lines 25202679 2014 up-regualted
miR-2117 Ascorbate approved 54670067 Microarray Metastatic melanoma cell lines 25202679 2014 up-regualted
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-2117 Imatinib 5291 NSC743414 approved resistant High Chronic Myelogenous Leukemia cell line (K562)
hsa-miR-2117 Vemurafenib 42611257 NSC761431 approved resistant cell line (LM16)
hsa-miR-2117 Vemurafenib 42611257 NSC761431 approved resistant cell line (LM43)
hsa-miR-2117 Vemurafenib 42611257 NSC761431 approved sensitive cell line (LM17)
hsa-miR-2117 Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)
hsa-miR-2117 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)

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