pre-miRNA Information | |
---|---|
pre-miRNA | hsa-mir-758 |
Genomic Coordinates | chr14: 101026020 - 101026107 |
Synonyms | MIRN758, hsa-mir-758, MIR758 |
Description | Homo sapiens miR-758 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Associated Diseases | ![]() |
Mature miRNA Information | ||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mature miRNA | hsa-miR-758-5p | |||||||||||||||||||||||||||||||||||||||||||||
Sequence | 15| GAUGGUUGACCAGAGAGCACAC |36 | |||||||||||||||||||||||||||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||||||||||||||||||||||||||
Experiments | SOLiD | |||||||||||||||||||||||||||||||||||||||||||||
Editing Events in miRNAs |
|
|||||||||||||||||||||||||||||||||||||||||||||
SNPs in miRNA |
|
|||||||||||||||||||||||||||||||||||||||||||||
Putative Targets |
miRNA Expression profile | |
---|---|
Human miRNA Tissue Atlas | |
miRNAs in Extracellular Vesicles |
|
Gene Information | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Tpgs2 | ||||||||||||||||||||
Synonyms | 5730437P09Rik, 5730494M16Rik, AI666318, Pgs2 | ||||||||||||||||||||
Description | tubulin polyglutamylase complex subunit 2 | ||||||||||||||||||||
Transcript | NM_001004361 | ||||||||||||||||||||
Other Transcripts | NM_001142697 , NM_001142698 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Tpgs2 | |||||||||||||||||||||
3'UTR of Tpgs2 (miRNA target sites are highlighted) |
>Tpgs2|NM_001004361|3'UTR 1 GCATTCTATCACCTCAGTAATATCCAGACACAGATGGCCCTGTGGCAGCGGCATTCTCCTGAGAGACCAAAGCCTTAGGC 81 CTTTGTTTCCACTGTCATTAGCTAGGGGCTGGGACGATCACACTGAATTCTCCTCAGGAGTTCCTTCCTCTGTAGGTTGT 161 GACAACCTCATCTCCAAAGCCAACGAAGACTTTCAGATGTACCTGAGCTTTCTTATGTCTGTACCCAATCCTTAACTTCT 241 TAGCAGTAATTAGAGAGGTCACCCTCCCAAATAAACCCATGGTTTGACTCATGAAGCATCAGTATCGACTAGTTATGTGA 321 CCATCTCAGTTAATGAAAATTGCACACCTTCAGTTGCATTCACCACACTTCAGCTATGCACCTGATGGTAGAGTCCATAT 401 TGGACAGCACAGAAAGGGGAAGGTTTTTGTCACAGAAGTGTTTTGGACAACATTGCATGTGAACACGTTCATACTGTCCT 481 TCAGATACAATAACGTAATGCCTTAGTTTCATACGCTTTATGTTTTGAGAAAACATTTTCAAAGACAGTCTTGTGTTCTT 561 TACAACCTAGACCAGGAATGCTTAAAGGTGTGATCCCTAGACTTAGAATTGTGCACCAAAGTCACTGGTATCATGTGGGG 641 ATAAGATGATTGGATTCCAGTTCTGATCTGCAAAATAAGAATTTCCAAGAAAAATTCCAGGTGTCAGGGTTTTCTTTTTA 721 AGACAAGGTCTCACTATATAGTTTAGGCTGACCTATAGCTCACACACCCTATATAGCTTCCTGACACACACACACACACC 801 CTGTCACTAACGTTTTAATTACAGATGTACAGTATCATGCGTAACATTGGATTTTTAGTCAATTTAACAGTACTTCTGAG 881 TTTGAAACTTTCAGCTTAAGATCTCAGTGGTTTCAAACCACAAAGTTTCTTTGTAATTGGGTAAAGCACGTATAACTTTT 961 TTGTTTTTTTTTTTTTGTTTTTTTTTTTTTGAAACAGGGTTTTTCTGTGTAGTCCTGGCTGTCCTGGAACTCATGGAGTT 1041 GTGGCTGCTAAATGACTTAGTGCATAGAGTTAGTTATCCTGTCCTTCCTTTGTAATGTGACCCTACGGTTGCTAATTATG 1121 ACCTTCCTGCTAGCAGCTGTGATCTCTTAAGTCGTTCATTTTTTGCTTGCAAGACACCAGTTGGGTTCTCACTTGTGCCT 1201 TTATACCTGTCCTTTCCTTGTGAGGTTCAGCTCTAGTCTGCCTCTACTCAAAGCTCAGTGAAGCTCCAACTCCCCTAGGT 1281 GAGCAGTGATTTTTTTGTGCTTCTCCAGAAAGCCATCCTAATCAATTCTGCATTAACCCAAATAAATGTGTCATATTTTA 1361 GGGCAATAAATTAGAATGAATGAGTAAAGTTGTGAAAATGTTTAACTTTAAAAATTGAGAAGACAATAATTGCCTTATTT 1441 TAGAGTCTGGATATCTTTCACTCATCTTACGGTCTGTGTCACAAAACCATCAAAATGAAATAGGTTACACATGAAGCCAT 1521 GAATGCCACTGGTCTTTTCTGCACCCAGAAACTATGTTGTACCCAGATACTACCTAACTGGATATCCCACCAATAACTGT 1601 CTTGATAAGGCTCTAAAAGACCAAAAAGTAACTCGTGAGACATGGTGGGATGTGAAACTTCTTACCCTGTATCTAGGAAA 1681 AGTTCTTCAACACCATTGATGCTGTTTCTTCAGATGTAAAATGAAAATTTATGGAAGGGCTATAAAACTATTAAATAATG 1761 CAAATGGAGTACACATGGAGATGCTTTCAGTAAGCAACAGCTTTTAAAATAGCATTCAGAAAGTCCATTTTCTCCCCATG 1841 AATTACCTAACCAACAGAACCAATTGCTTTACCAGATGATTTTTTGACAAAATTGATTATTTTAACACCTATCTTTACTG 1921 TCTATTTCAAAGCCATTAGCTTTCTTATAAAATCGCAAAGACATTTCATTCCTGAAGCAATTTGGTACTTTTGTGGTGTA 2001 GTTACCTCTCTGATAAAATGATTTCTAATCAAAACTAGAAATAGCTAAAGAATGGGGGATAACTGAAATTTAATTGGATG 2081 ACAGCCTCCCACAGCTTCTGTTAGACCTCCTGAGGAGAAGGATCAAGGCTCTCATTTTCCCAACTGACCACCCAGCCCTG 2161 TCACAGAGCTACCTACAAACCTGGCCCTTAGTGCATAGTTTGTTCTACTGTGTTTTCAGGACCTGATGGCTTAATACACA 2241 TAAGTTTACCCGTGTGTATTTCTGCAGGCCAGAAGTTCAATGTCAGTTTTATAGAGCCAAAATCTAGTTGTCACTGGGCC 2321 AGGACTTGGGAGGCTCCTATAGAGTCTGTCCCTGCTTCCTCCTGCAGATTGTTGTTTGGGTTCATATCACTTCAGTCCTG 2401 TTGCTTTCACTTCGTCTTCTCTTTCTCCAAATCTCTTCTGACTGTCCTTTATAAGGACACTTATGATTGGATTTGAGGCA 2481 TGTCGGACACTACAGGATGATCTTCTCACACCTGCAAGGAATCCTTACAAAGGAATTGTACATTTCAGAGATTAGAACCG 2561 GAGGCCCCAGAGATGTTGAGTCAATGTATTACAGTAGTGTTTGCTACTTCACCATTTCAAGGGACTGTGGATATAAATGA 2641 TTGTTTTGAAGGCCATAACGGATCTATAAGCCACTTTAGAGCTGGGAAACTAAGCTGCCGTTTGTACCCCTGCTCATCTT 2721 CTCTACATTGTGTTCAACAATGAACCTTGAAGAAAATAAAACCCAGGTCTCCAGCCTTCCCCAGCCCCGTGCATGCGCAT 2801 TCTCCTTCACCCCCAATTCCTTTTGAGTAAGTTACTTGTTAAAATAAATCCTTAAGGTTCAAAACAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
miRNA-target interactions (Predicted by miRanda) |
|
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
---|---|---|---|---|---|---|---|
miRNA:Target | ---- | ||||||
Validation Method |
|
||||||
Conditions | hMSC | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
HITS-CLIP聽 data was present in GSM1013116. RNA binding protein: AGO. Condition:hMSC-replicate-5
... - Balakrishnan I; Yang X; Brown J; et al., 2014, Stem cells (Dayton, Ohio). |
||||||
miRNA-target interactions (Provided by authors) |
|
||||||
Article |
- Balakrishnan I; Yang X; Brown J; et al. - Stem cells (Dayton, Ohio), 2014
Regulation of hematopoietic stem cell proliferation, lineage commitment, and differentiation in adult vertebrates requires extrinsic signals provided by cells in the marrow microenvironment (ME) located within the bone marrow. Both secreted and cell-surface bound factors critical to this regulation have been identified, yet control of their expression by cells within the ME has not been addressed. Herein we hypothesize that microRNAs (miRNAs) contribute to their controlled expression. MiRNAs are small noncoding RNAs that bind to target mRNAs and downregulate gene expression by either initiating mRNA degradation or preventing peptide translation. Testing the role of miRNAs in downregulating gene expression has been difficult since conventional techniques used to define miRNA-mRNA interactions are indirect and have high false-positive and negative rates. In this report, a genome-wide biochemical technique (high-throughput sequencing of RNA isolated by cross-linking immunoprecipitation or HITS-CLIP) was used to generate unbiased genome-wide maps of miRNA-mRNA interactions in two critical cellular components of the marrow ME: marrow stromal cells and bone marrow endothelial cells. Analysis of these datasets identified miRNAs as direct regulators of JAG1, WNT5A, MMP2, and VEGFA; four factors that are important to ME function. Our results show the feasibility and utility of unbiased genome-wide biochemical techniques in dissecting the role of miRNAs in regulation of complex tissues such as the marrow ME.
LinkOut: [PMID: 24038734]
|
Experimental Support 2 for Functional miRNA-Target Interaction | |||||||
---|---|---|---|---|---|---|---|
miRNA:Target | ---- | ||||||
Validation Method |
|
||||||
miRNA-target interactions (Provided by authors) |
|
||||||
Article |
Elucidation of transcriptome-wide microRNA binding sites in human cardiac tissues by Ago2 HITS-CLIP.
- Spengler RM; Zhang X; Cheng C; McLendon JM; et al.- Nucleic acids research, 2016
MicroRNAs (miRs) have emerged as key biological effectors in human health and disease. These small noncoding RNAs are incorporated into Argonaute (Ago) proteins, where they direct post-transcriptional gene silencing via base-pairing with target transcripts. Although miRs have become intriguing biological entities and attractive therapeutic targets, the translational impacts of miR research remain limited by a paucity of empirical miR targeting data, particularly in human primary tissues. Here, to improve our understanding of the diverse roles miRs play in cardiovascular function and disease, we applied high-throughput methods to globally profile miR:target interactions in human heart tissues. We deciphered Ago2:RNA interactions using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across >2200 target transcripts. Our initial exploration of this interactome revealed an abundance of miR target sites in gene coding regions, including several sites pointing to new miR-29 functions in regulating cardiomyocyte calcium, growth and metabolism. Also, we uncovered several clinically-relevant interactions involving common genetic variants that alter miR targeting events in cardiomyopathy-associated genes. Overall, these data provide a critical resource for bolstering translational miR research in heart, and likely beyond.
LinkOut: [PMID: 27418678]
|
CLIP-seq Support 1 for dataset GSM1015448 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | BCBL-1 / BCBL-1 mRNA |
Location of target site | ENST00000334295.4 | 3UTR | AAACGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUUA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 22927820 / GSE41357 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM1048188 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | Hela / Hela_AGO2_CLIP_ptb_knockdown |
Location of target site | ENST00000334295.4 | 3UTR | AAACGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUUAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23313552 / GSE42701 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM1013116 | |
---|---|
Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | hMSC / hMSC-replicate-5 |
Location of target site | ENST00000334295.4 | 3UTR | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUUAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 24038734 / GSE41272 |
CLIP-seq Viewer | Link |
62 hsa-miR-758-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
![]() |
![]() |
|||||||
Strong evidence | Less strong evidence | |||||||||||
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|||||
MIRT085323 | MORC3 | MORC family CW-type zinc finger 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT089441 | STAMBP | STAM binding protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT089456 | TET3 | tet methylcytosine dioxygenase 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT111856 | CCND1 | cyclin D1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT184933 | ZNF268 | zinc finger protein 268 | ![]() |
![]() |
2 | 2 | ||||||
MIRT215288 | CREBRF | CREB3 regulatory factor | ![]() |
![]() |
2 | 2 | ||||||
MIRT237300 | LPP | LIM domain containing preferred translocation partner in lipoma | ![]() |
![]() |
2 | 2 | ||||||
MIRT238446 | MYO10 | myosin X | ![]() |
![]() |
2 | 4 | ||||||
MIRT273827 | RPL41 | ribosomal protein L41 | ![]() |
![]() |
2 | 2 | ||||||
MIRT282703 | HOOK1 | hook microtubule tethering protein 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT347970 | ZNF850 | zinc finger protein 850 | ![]() |
![]() |
2 | 2 | ||||||
MIRT371076 | KLF3 | Kruppel like factor 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT464339 | USP6NL | USP6 N-terminal like | ![]() |
![]() |
2 | 2 | ||||||
MIRT470034 | PTP4A1 | protein tyrosine phosphatase type IVA, member 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT477506 | ELL2 | elongation factor for RNA polymerase II 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT482886 | CACNA2D3 | calcium voltage-gated channel auxiliary subunit alpha2delta 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT492606 | POLR3E | RNA polymerase III subunit E | ![]() |
![]() |
2 | 2 | ||||||
MIRT502294 | GNG12 | G protein subunit gamma 12 | ![]() |
![]() |
2 | 6 | ||||||
MIRT507600 | DCTN4 | dynactin subunit 4 | ![]() |
![]() |
2 | 4 | ||||||
MIRT510728 | SON | SON DNA binding protein | ![]() |
![]() |
2 | 6 | ||||||
MIRT514065 | KCNJ6 | potassium voltage-gated channel subfamily J member 6 | ![]() |
![]() |
2 | 8 | ||||||
MIRT519718 | ZNF512B | zinc finger protein 512B | ![]() |
![]() |
2 | 4 | ||||||
MIRT520890 | STRN | striatin | ![]() |
![]() |
2 | 2 | ||||||
MIRT521760 | PPIL1 | peptidylprolyl isomerase like 1 | ![]() |
![]() |
2 | 6 | ||||||
MIRT526874 | ERCC8 | ERCC excision repair 8, CSA ubiquitin ligase complex subunit | ![]() |
![]() |
2 | 2 | ||||||
MIRT530232 | WSB2 | WD repeat and SOCS box containing 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT532003 | ACTR2 | ARP2 actin related protein 2 homolog | ![]() |
![]() |
2 | 2 | ||||||
MIRT533371 | UBE2D4 | ubiquitin conjugating enzyme E2 D4 (putative) | ![]() |
![]() |
2 | 4 | ||||||
MIRT547106 | PIGW | phosphatidylinositol glycan anchor biosynthesis class W | ![]() |
![]() |
2 | 2 | ||||||
MIRT548189 | FOXA1 | forkhead box A1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT552935 | VKORC1L1 | vitamin K epoxide reductase complex subunit 1 like 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT560085 | ZNF195 | zinc finger protein 195 | ![]() |
![]() |
2 | 2 | ||||||
MIRT561726 | PPP2CA | protein phosphatase 2 catalytic subunit alpha | ![]() |
![]() |
2 | 2 | ||||||
MIRT562713 | ZNF415 | zinc finger protein 415 | ![]() |
![]() |
2 | 2 | ||||||
MIRT562761 | ZNF846 | zinc finger protein 846 | ![]() |
![]() |
2 | 2 | ||||||
MIRT564159 | ZNF117 | zinc finger protein 117 | ![]() |
![]() |
2 | 2 | ||||||
MIRT565673 | SETD5 | SET domain containing 5 | ![]() |
![]() |
2 | 2 | ||||||
MIRT565718 | SESN3 | sestrin 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT566026 | RFX1 | regulatory factor X1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT569048 | ZNF655 | zinc finger protein 655 | ![]() |
![]() |
2 | 2 | ||||||
MIRT570367 | UBE2V1 | ubiquitin conjugating enzyme E2 V1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT570410 | TMEM189-UBE2V1 | TMEM189-UBE2V1 readthrough | ![]() |
![]() |
2 | 2 | ||||||
MIRT570443 | TMEM189 | transmembrane protein 189 | ![]() |
![]() |
2 | 2 | ||||||
MIRT571738 | RNF11 | ring finger protein 11 | ![]() |
![]() |
2 | 2 | ||||||
MIRT575042 | Tpgs2 | tubulin polyglutamylase complex subunit 2 | ![]() |
![]() |
2 | 4 | ||||||
MIRT614330 | ZDHHC22 | zinc finger DHHC-type containing 22 | ![]() |
![]() |
2 | 2 | ||||||
MIRT617629 | RAB3IP | RAB3A interacting protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT621667 | UBE4B | ubiquitination factor E4B | ![]() |
![]() |
2 | 2 | ||||||
MIRT639906 | SRGAP2 | SLIT-ROBO Rho GTPase activating protein 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT651436 | XRCC5 | X-ray repair cross complementing 5 | ![]() |
![]() |
2 | 2 | ||||||
MIRT683853 | ZNF208 | zinc finger protein 208 | ![]() |
![]() |
2 | 2 | ||||||
MIRT684841 | TPGS2 | tubulin polyglutamylase complex subunit 2 | ![]() |
![]() |
2 | 5 | ||||||
MIRT689347 | ZNF83 | zinc finger protein 83 | ![]() |
![]() |
2 | 2 | ||||||
MIRT692492 | SPIN4 | spindlin family member 4 | ![]() |
![]() |
2 | 2 | ||||||
MIRT695711 | OLA1 | Obg like ATPase 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT698219 | TMEM248 | transmembrane protein 248 | ![]() |
![]() |
2 | 2 | ||||||
MIRT711560 | FAM20B | FAM20B, glycosaminoglycan xylosylkinase | ![]() |
![]() |
2 | 2 | ||||||
MIRT712867 | TMEM67 | transmembrane protein 67 | ![]() |
![]() |
2 | 2 | ||||||
MIRT722956 | TSPAN1 | tetraspanin 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT723622 | SOBP | sine oculis binding protein homolog | ![]() |
![]() |
2 | 2 | ||||||
MIRT724176 | ABCF2 | ATP binding cassette subfamily F member 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT755363 | LMBR1 | limb development membrane protein 1 | 3 | 1 |
miRNA-Drug Associations | |||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|