miRTarBase - #MIRT577239


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pre-miRNA Information
pre-miRNA mmu-mir-363   
Genomic Coordinates chrX: 52741693 - 52741767
Synonyms Mirn363, mmu-mir-363, Mir363
Description Mus musculus miR-363 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-363-5p
Sequence 7| CAGGUGGAACACGAUGCAAUUU |28
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Zfp933   
Synonyms 2810408P10Rik
Description zinc finger protein 933
Transcript NM_198619   
Expression
Putative miRNA Targets on Zfp933
3'UTR of Zfp933
(miRNA target sites are highlighted)		 
>Zfp933|NM_198619|3'UTR
   1 GCAAAGCAATATGGTAAAGCCTTTGCATATAGCAGTAGCCTTAGAAATCATGAGAAAAATCAGCCTGCAGAGAAACCCCA
  81 CAAATGTAGTGAGTGTGGTAAAGTTTTGTTCTTCATTGTATCTTTATACAAGAGGAAAATCTGCAGTGTGTGATCAGTCT
 161 GCTAAAGACTGCAGATCCCAGTAGTCGTTGAGGTTCTGAAAGAACTACGGAAGGAAGTCTATGACTAGAAGGGGTTTGGT
 241 GTGATCTTTAGCCAACACACATTCAGTTACACAGCATTGTTTATTCTAGAGACAAAAGTGTAACAATGTGTCAACAATAT
 321 CTTCAAGCATTGTGATGTCACTATTTTGGTTGCACTTTTTTTTCCCACTCATTTTTGCACGTTGAACCATTTCTGCATCT
 401 CTGGGATGAAGCCAAGTTGAACATCATATGTGATACTTTTGGTCTTTTCTTTGAATTTGCAAGTATCTTACCATTTTTAC
 481 ATCTGTGCTCATGTGGGAAATTGATCTATAATACTCTTTTTTTTTTTTTTTCCCCTTTGAGTCTTTGTGTGCTTTGGGTA
 561 TCAGAGTAACTGTGGCTTCATATAATGAATTGGGTAATGTTCCCTTTTTCTATTTTTAGAATTTTGTAAAGAATATTTTC
 641 CTCGCCGGGCGTGGTGGCGCACACCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGGGGATTTCTGAGTTCAAGGCCAG
 721 CCTGGTCTACAAAGTGAATGCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGAAAAACCAAAAAAACAAACAAA
 801 CAAAAGAATATTTTCCTCCTGAAGGGGCAAATCTAGACGCCAAGATTTTGGTGAAGTTAGTTACCTCTGCTCCTGTGTGT
 881 ACCAAACCTTCAGTTGCAGTGCCATCTATTTGTATTTCCAGCTTTTGTTTTTCATCATTTCTAGCAGTTGGCGAAAATAC
 961 CTGTTTTCTGATCTCTCCTGAATTTGTTCACTTATCTACAGAAGTGACTCTGACCTTGAGCACATGCGGGCTAGTGTTGT
1041 TTTTTAACAACAGGCATTGCATTAACTTGTTTAATTGTTATGGGTGAGTTTCTTTGAGACCAACAGGATATGATTGAACT
1121 GAATTTGATTCTGGGCCCCATAGGGTGTTTCTGAAGGCAGAGCTTTACCTTGCCTATCACTTTTTGATCTGCATTCATTA
1201 GTTCAGTGCTGACCTTTGCCACACCTTTTTCGTACTCTAGAAGGCTGGGGCCTTCCTTTTGGATTATCTGTGGGGGAAAA
1281 AAATCCTTTCTTGGAATGCCTGGCCTACAATCCCTTGTCAGGCGTCCAGGGTACCACACTGAGAACAGCTGGCATTTGGA
1361 TGGTTCCTGAACTTTGGGAAATGACTTTTCCTATCAGTGTGGCATCATAAACTTGAGATCCAGCATCACCCATATTTCTA
1441 ATCCATCCCTCTGTTGGTGCTGAGCTTGCCTGTGAAGGTCTAATCAATGTTTTGCGTTCTGAATTAGCATGTTCAAAAGC
1521 CAAATTTTACAATTAGCATTTTTCTGACTCCTAAATCTGATACTATTCCATTTACAGCTGAAGTAAATTTTTGCAAAACA
1601 TCAGAGAAGGCTTCTTTTGGGCTGTGTATAATTTTAGTAAACGACTCAAACCCCTTTCCTGAACCTTGAACCGCATCACA
1681 AGCATTTAAAGCTGCCAAATAACATAGTGCCATGATGTGATTATGAAAATCAGGCTGCCTGTGTAACTCAGCCTAGTAAC
1761 CTTCACCTCACAACTGATCTTGGGAAATATCCATCCCCCTAGCCTGATTGCATTGTGCTATGGCCCTAGCTTCCTCTTTT
1841 AAATTTTTATTTATTTATTTAATTTTTTTACATTTTAAAATTTTTATTTATGAGTGCACACCAGAAGAGGGCATCAGATC
1921 CCATCACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCGTCTATTGCTCTTA
2001 ACCACTGAGCCATCTCTCCAGTACCTGTCCCCCAAGCCCCCCAGCTTCCTCTTTTCACCACGTTTGCCAGGATGATCTAT
2081 TCTCCTTGCAAAGATACAAAAGATTAGGAACAATTATATTGTAGGGTGTTATTACACCAGAGGGTATTACAACCCCAAAG
2161 CAGAAGGAGAGAGCAAGATTAAGAGGAGGAGGAAGGACAGTAGAGTAGAACTTAGAAATTAAAAGGGAACTTTGAAATTA
2241 TGAGAGTAATACACAAAGTGCAGAGAGGAGGGAAAAAGAATCCATATAGAGAGTAGAGAGAGCAGGCAGGCTTCTCCTTA
2321 TTATGGGTGGGAACAGGTTGGTTTGTTTGTTTGATTGATTTTCGAGACAGGGTTTCTCTGTGCAGCTCTGGCTGTCCTGA
2401 AACTCACTCTGTAGACCAGGCTGGCCTCGAACTTAGAGATCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAAGCGT
2481 GCGCCACCATAAAATACATTTTATGGATGTCTCTACTTCTTAGGGTGTTAGTGTTTTGTTAGTGTAAGTGCAAAGAGTCA
2561 AAAGATGTTTTCCAGAACGTAAATTAATGTTGTTTAGATATGCAAAGTGTGATTACATTACGTGACTGTATAGCCATAAA
2641 AAGGGGTAGTGATGCTGGTATAATCTCAGAATTCCGAACCCTGGGGCAGGTGTATTCCTTTTCATTTGAAGCCAGACTAC
2721 TCTACATTAAAGTGTCATTCCACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uuuaacGUAGCACAAGGUGGAc 5'
                | | | | ||||||| 
Target 5' ctcgaaCTTAGAGATCCACCTg 3'
 2426 - 2447 148.00 -14.60
2 
miRNA  3' uuUAACGUAGC-ACA-AGGUGGAc 5'
            ||| :|| | ||| ||:||:| 
Target 5' acATTTTATGGATGTCTCTACTTc 3'
 2496 - 2519 132.00 -7.80
3 
miRNA  3' uuuaaCGUAGCA--CAAGGUGGAc 5'
               || | ||    |:||||| 
Target 5' actcaGCCTAGTAACCTTCACCTc 3'
 1746 - 1769 127.00 -7.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' uuuaacguagCACAAGGUGGAc 5'
                    | | ||||||| 
Target 5' ---------aGAGAUCCACCUg 3'
 		1 - 13
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' uuuaacGUAGCACAAGGUGGAc 5'
                | | | | ||||||| 
Target 5' -----aCUUAGAGAUCCACCUg 3'
 		1 - 17
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM622571
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1B
Location of target site NM_198619 | 3UTR | AGAGAUCCACCUGCCUCUGCCUCCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1385343
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_DM_Ago2_CLIP-Seq_myotubes
Location of target site NM_198619 | 3UTR | ACUUAGAGAUCCACCUGCCUCUGCCUCCCGAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
76 mmu-miR-363-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577239 Zfp933 zinc finger protein 933 2 4
MIRT578026 Pfas phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) 2 2
MIRT578034 Pde4c phosphodiesterase 4C, cAMP specific 2 2
MIRT578102 Nup133 nucleoporin 133 2 2
MIRT578143 Nfatc4 nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4 2 6
MIRT578431 Nmrk2 nicotinamide riboside kinase 2 1 1
MIRT579036 Cts8 cathepsin 8 2 4
MIRT579251 Car5b carbonic anhydrase 5b, mitochondrial 2 4
MIRT579607 4930519G04Rik RIKEN cDNA 4930519G04 gene 2 4
MIRT581397 Rbms2 RNA binding motif, single stranded interacting protein 2 2 4
MIRT585497 Ttc26 tetratricopeptide repeat domain 26 2 4
MIRT585507 Tspan31 tetraspanin 31 2 4
MIRT585583 Tox4 TOX high mobility group box family member 4 2 4
MIRT585842 Slc3a2 solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 2 2
MIRT586452 Nploc4 NPL4 homolog, ubiquitin recognition factor 2 4
MIRT587010 Gngt1 guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 2 4
MIRT588115 Aco1 aconitase 1 2 4
MIRT589171 Rab11b RAB11B, member RAS oncogene family 2 2
MIRT589338 Ostf1 osteoclast stimulating factor 1 2 4
MIRT589488 Mzt1 mitotic spindle organizing protein 1 2 4
MIRT589617 Lrrc58 leucine rich repeat containing 58 2 4
MIRT589816 Hnrnpab heterogeneous nuclear ribonucleoprotein A/B 2 4
MIRT590086 Evi2b ecotropic viral integration site 2b 2 2
MIRT590799 2810474O19Rik RIKEN cDNA 2810474O19 gene 2 2
MIRT590993 Slc28a3 solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 2 4
MIRT592906 Mmachc methylmalonic aciduria cblC type, with homocystinuria 2 4
MIRT593320 Slc25a51 solute carrier family 25, member 51 1 2
MIRT594758 Ttn titin 2 2
MIRT594872 Magt1 magnesium transporter 1 2 2
MIRT595323 Gfap glial fibrillary acidic protein 2 2
MIRT595614 Abce1 ATP-binding cassette, sub-family E (OABP), member 1 2 2
MIRT596863 Ttc23 tetratricopeptide repeat domain 23 2 2
MIRT597329 Senp5 SUMO/sentrin specific peptidase 5 2 2
MIRT597745 Polr1b polymerase (RNA) I polypeptide B 2 2
MIRT597779 Plin3 perilipin 3 2 2
MIRT597900 Paxip1 PAX interacting (with transcription-activation domain) protein 1 2 2
MIRT598014 Nop16 NOP16 nucleolar protein 2 2
MIRT598196 Mrgprb1 MAS-related GPR, member B1 2 2
MIRT598360 Man2a2 mannosidase 2, alpha 2 2 2
MIRT598933 Git2 G protein-coupled receptor kinase-interactor 2 2 2
MIRT599064 Epb4.2 erythrocyte membrane protein band 4.2 2 2
MIRT599072 Eif2b1 eukaryotic translation initiation factor 2B, subunit 1 (alpha) 2 2
MIRT599119 Dynll1 dynein light chain LC8-type 1 2 2
MIRT599271 D14Abb1e family with sequence similarity 208, member A 2 2
MIRT599613 Birc5 baculoviral IAP repeat-containing 5 2 2
MIRT599909 Abcc9 ATP-binding cassette, sub-family C (CFTR/MRP), member 9 2 2
MIRT600060 Smco1 single-pass membrane protein with coiled-coil domains 1 1 1
MIRT600067 Knop1 lysine rich nucleolar protein 1 1 1
MIRT600320 Stxbp5l syntaxin binding protein 5-like 2 2
MIRT600337 Strbp spermatid perinuclear RNA binding protein 2 2
MIRT600524 Ppargc1a peroxisome proliferative activated receptor, gamma, coactivator 1 alpha 2 2
MIRT600646 Med18 mediator complex subunit 18 2 2
MIRT600683 Mapre1 microtubule-associated protein, RP/EB family, member 1 2 2
MIRT600802 C5ar2 complement component 5a receptor 2 1 1
MIRT601265 9930104L06Rik RIKEN cDNA 9930104L06 gene 2 2
MIRT601726 Rfxank regulatory factor X-associated ankyrin-containing protein 2 2
MIRT601751 Rasl12 RAS-like, family 12 2 2
MIRT601888 Pctp phosphatidylcholine transfer protein 2 2
MIRT602148 Irgm2 immunity-related GTPase family M member 2 2 2
MIRT602904 Fam219b family with sequence similarity 219, member B 1 1
MIRT603138 Ufd1l ubiquitin recognition factor in ER-associated degradation 1 2 2
MIRT603213 Tnfrsf26 tumor necrosis factor receptor superfamily, member 26 2 2
MIRT603268 Svopl SV2 related protein homolog (rat)-like 2 2
MIRT603291 Spib Spi-B transcription factor (Spi-1/PU.1 related) 2 2
MIRT603922 Hist1h2br histone cluster 1 H2br 2 2
MIRT603924 Hist1h2bq histone cluster 1, H2bq 2 2
MIRT604014 Fut4 fucosyltransferase 4 2 2
MIRT604105 Dll3 delta-like 3 (Drosophila) 2 2
MIRT604166 Cyb5d2 cytochrome b5 domain containing 2 2 2
MIRT604313 Ccdc127 coiled-coil domain containing 127 2 2
MIRT605041 Efr3b EFR3 homolog B 2 2
MIRT605716 Gpr157 G protein-coupled receptor 157 2 2
MIRT605741 Gipc1 GIPC PDZ domain containing family, member 1 2 2
MIRT605747 Fam96a family with sequence similarity 96, member A 2 2
MIRT606125 Tnfrsf10b tumor necrosis factor receptor superfamily, member 10b 2 2
MIRT606693 Cnnm3 cyclin M3 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-363 Emodin NULL 3220 Microarray K562 cells 23744534 2013 down-regualted
miR-363 Emodin NULL 3220 Quantitative real-time PCR K562 cells 23744534 2013 down-regualted
miR-363 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-363 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-363 Bicalutamide approved 2375 Microarray prostate 22674191 2012 down-regulated
miR-363 17beta-estradiol (E2) approved 5757 Microarray rat breast 17700064 2007 up-regulated
miR-363 Acetaminophen approved 1983 Microarray rat liver 17965554 2007 up-regulated
miR-363 Carbon tetrachloride NULL 5943 Microarray rat liver 17965554 2007 up-regulated
miR-363 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 down-regulated

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