miRTarBase - #MIRT577961


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pre-miRNA Information
pre-miRNA mmu-mir-743b   
Genomic Coordinates chrX: 66777256 - 66777332
Description Mus musculus miR-743b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-743b-3p
Sequence 46| GAAAGACAUCAUGCUGAAUAGA |67
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Pm20d2   
Synonyms Acy1l2, Gm424
Description peptidase M20 domain containing 2
Transcript NM_001034867   
Expression
Putative miRNA Targets on Pm20d2
3'UTR of Pm20d2
(miRNA target sites are highlighted)		 
>Pm20d2|NM_001034867|3'UTR
   1 AAGGAAACCCTAGGGACTGGTCAGGAAATGTTACGTTTTGTTTGTTTTTTACCTCTCATGTTTTTCAGTGAAAGAGAGCA
  81 TGCTTGTTTTTCAGTCCTGGTAAGTGAGGACAGATATAGGGAAGAAGACACGAATGCACGTCTAGATTTAGTGGAGTTTG
 161 TGATACTGCAGGCGTTGGTGCGTAACGCCATCTATTACGCCAGATGATACCTGATCCTGTAACATTCGGTAATGTTTTGT
 241 AAGTGTATCTGCTGTATGGTGTTACAAGTCCCACGGGTTTCAGAAGGTGACACTCATGAACTCTGTGGTTCAGAGTAGGG
 321 AATGTCTTCTCGGCAGTTTAAATCGAGGGGGAGACTTGTTTTAAGTCTAACTGTATCTGTTTGGGTCTAAAGAAAAACTT
 401 GAAATAAACATATGCTTGTGGGACACTCCTAGAATCCCCAGTACAAAGAATTAGCTCTACTTTTAACACTTAGAGAAGGG
 481 GCGAGGAGGAGTGGAGGGAATGGTGGAAGAGGGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGATCTTTACTTACATGTA
 561 ATTACTCCAAGAAGTGGCATAGGAACAGTGGGTAGTAACAGGAAAAATGGCAGAAAGGCACAGTTGAGCCTTTCTAGCAG
 641 GGTAGGGTGTTGTGTGTTGTGTTACAGGAGTTGGCCGGAGTACAGACTAGGTGTGCTCTGTTCTCCAGTGTCCCACACGG
 721 TCCCCAGTCCCTAGCAGCCTCAGAAGAGCCCCAAAGCCCTGTGCTGGCCCAGTAGTAATGTGAACCCATGGCCTTGGAAA
 801 GGAGGCTCCGTAGCTCCTAATGAGACTTAGTTAAGCTTCCTGCAGTTTCTCTGGACTTTCTTTTGTGCCTTGTCTGCTCG
 881 CTCCCTGGTTTGTGTCACCACAGCATTTGTAGAGCCACAGGAGGGAGTTGTTTTTACAAGGAAGCCAGGATTCGAGCTGG
 961 AAATCTGAAACATTTCAGTGGTTGTCAGTAAAATCACTGTCAGTAATGCCCTGGAAAGGAGCGAGTCACATAAAATCAGC
1041 AGTTCTTCATGGTCCTCACTGTAGCACAAGTTTCTTTGGTTTTGGTGGGGCTGAGTTTGAGACAGGTTTTCCCTTCTGCC
1121 CGCCTCCTCCCCACTCTCTTCCCTCTGCTGCTGCCTCTCAGATGCTGTGATTATAGGCTCGAGCACAGCACACCTGCCAT
1201 CTAGTGTGAACTTGCACATGATTAAAGTGTGAAGAAGCCGGTAAAACCAACTCGTGGGCATTTGAGGAGGCGGTTCAGAG
1281 TCAGAAGTGGAAGGGCACAGGGAAGTGCATTTGCTTCTGTGGTGGAAGCAAGTCACTTTCCCTCAGTGACACCAGCTGCC
1361 ACTTAGAAGGTTAGCCATCTTTGACCCTTCTAGCCTCTGATTTCCTGTTTGATGTGTACTTTAGAACAGGGAGCGGGGTT
1441 GTATTGGAGGGCTAACATCTGAGAACTGCTCTCTGCTGTTCTCTGGCTTGACATAGGCTGGTGTTTGCTTGATTACTTGC
1521 CTTTGTGCAGCTAGTATACTAAGAATTGTTTTGCATTTTAGAGGTTAAAAAAAAAACCCCAAAAACAAAAAACCACCATT
1601 ACCACAAAGCATAAAGTATTTACTGTCTGACCTGTCAGGAAAAAGAAACCTCTCAGAGCCAGTCAATCGGGCAGTTTCTG
1681 ATTTCTGTTCTCTTGAATTTTCTGGGGAAAGCTTCAAGTCCTGCATTGGAAGCACTTTAGTGTAGATAGGGGCTGCCTGA
1761 CAGAGTGATCAGGACTGTTCCACCCTCTACTTTGTCCTTCTAGACAGAAGCCTGTTTCAGCTCCCCTCACCCTGCCCCAC
1841 AAGTCCTCAGCCTTGCCTCTGCACACCACCCGTTCTGTCTTTGCGTAACATTGAGAACTCAGTCCTCCATGACACCTTGT
1921 GTCTGGATACTCACACCCGAGTTTATCATATTAAATACAAATTTTGTATAAAGAGGGAGTATCAATGAAAACTTGAATCA
2001 AAAAATAAGACATGCCAGAGATGTGGTTTTTTTTTATTGATGGACTTGGTCTCCTAAAAGGCTGTTTTATTAGTTCCTCA
2081 CATCTTGAGGGATAGGTTTTTTTGTTGATTGATTTAGTTTTGGTTTTTGCAGGGCTTGATATTGAGACCAGGGCTCTAGC
2161 ACAGAGCTGCATCCCCAGCTCCAGGGCAGGGCATTGGTTGCATTTGCTATAATCTTGACACCATCCTAGAACAGCTGTTG
2241 TTATTTATGCAGCCCAACCTCTGAGAATCATGTGTATTCCGTGTCTGGTTTCCTTTTCCTTTTTTTTTTTTTAAGTAAAA
2321 TTAAATTTGACTGATGTATAAACAATGTCGGGTTTAGGTAGGTAAGGAAGGGTGTGTTTCTTGTTGGAGGTTGCTGCTGT
2401 GATTTTACAATTTGCTGTCTTATTGTCCAATGGTTTACCATTTTGAACCACTTACACCATGTCCTTACAAATGCCTTTTC
2481 CTGTCACCTGGGAGAGGTGTTACGTCAATGCATTCTAGAATGAGTAAGCTACACTTAGCAGCTTAAATGTAATTACAATC
2561 TGAAATTTATTTTCCTTTACCAGCTTAATTCTAAGGAAGCTTGAATATGGGACCTGTGCAGTACAGGTGTTAAAGTTATG
2641 TTTCCTTTTTCCTAAATGCCTGAGACCAGCGGTGTTTCAGATTTTAGATTAAAAAAATGTTTTGAAATATTTGCATATAT
2721 ATTATGTAATCTATATTTTATACATATATATGTATATACAAACAGGGAGGGGGAAGATAGAGATGGAACCCAGCTAGAGC
2801 TATAAAAATCCCTTTGTGTATCACATGGGCCTGCCCTATGCACACAGCTTGAAGGACGGTGTGCTCAGTGTTTTAGTGTG
2881 TCTGAGAGTTAACTGACCTGTGTGCACAGGAGGCCAGTTGTGCAGTTCTCACCTAGGTCGTGCCACTCACAGTTGGATTT
2961 CTGGAGCATTTTGGTCAGTCAACCTGGACTGCGAACTAGTAAGTTGCTGTGTATTCTGTGACTGTAGCTAATATTTAGCT
3041 TGCCTCAAATTTTTTGAGCTGATATTTAGTCTTAACAAATTCTCCTTATTGGATGAACTGCACAGGAAGTTTTTCTTTTC
3121 AACCACGTCTCTCTATGACATAAACGTTGGGGTACTTAGGGTAAAGTAGACACTGCATGTGTTTATGAAGGTGCAAACAG
3201 AAAGAAGGGTGTCAAGTGCTACAGTTGCATTGCTGTTGCTGTTTGTAAACTGTGCCGTCTCAGCGTTTGGGTGAGTAAAT
3281 GTTTGCAACCACTGGAGAAGAGTAAAGGCCCTGTGTGTGGTGCTACATCCTCCTAGGATTTAGTTCACACAGACTATGCC
3361 AGGATGGAGGGGCACCTGAAAACTGCCCTGGGAGTTTTGGAGGAAGGGCTTCTAAAATACTTAAAAATACGTGAATCACT
3441 TCAGTCTGTTTAAACGGAAAAAAATATTTGATACCAGGTCTTTATATATATATATAAATATATATAAAAGAATAGTTGTC
3521 TCAAGAGGAATAAAATCTGGAGTGTAAAAAAAATGTTAAAAAAAAAGAATACTTGTCTCACTTTATAATATTTTTTAAAG
3601 GAAAAAAGAAAACGTTTCCAATAGCTTTTGTGTTCTGTGAGCTCTCACTTTTTACCTGAAGCAGTCTTCTGTAGCATGTC
3681 CCACAGCATCAGAGTAACTTGATGGTGACTATCATTTTTGTGCCCATTATCAGGCTCCAGTCATCTGTGAGAGGGGATAA
3761 ACTACCTTGCTACTAACTTAGGCCTTTTGTGATCTAATGACATAGCTGAGGGTGGTGTGTTCGGGTATATAAGAGGTTAG
3841 TATATTCAAAAACAATCTCTGCTATTGTGGTTATAATTATTTTTGTAGTGTTTTAATAGATGCACATTAAAGTTTGTAAA
3921 AAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agauaagucGUACUACAGAAAg 5'
                   |:|  ||||||| 
Target 5' cacaccaccCGTTCTGTCTTTg 3'
 1862 - 1883 145.00 -6.80
2 
miRNA  3' agauaagUCGUAC-UACAGAAAg 5'
                 ||||||  |||:||| 
Target 5' gaaagagAGCATGCTTGTTTTTc 3'
 70 - 92 143.00 -14.10
3 
miRNA  3' agAUAAGUCG-UACUACAGAAAg 5'
            | ||:| |  | ||||:||| 
Target 5' gtTTTTTACCTCTCATGTTTTTc 3'
 44 - 66 140.00 -7.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM1385343
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_DM_Ago2_CLIP-Seq_myotubes
Location of target site NM_001034867 | 3UTR | CUUUAUAUAUAUAUAUAAAUA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
101 mmu-miR-743b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577961 Pm20d2 peptidase M20 domain containing 2 2 2
MIRT578387 Klra2 killer cell lectin-like receptor, subfamily A, member 2 2 6
MIRT579831 Zfhx3 zinc finger homeobox 3 2 8
MIRT580616 Syp synaptophysin 2 2
MIRT580731 Srrm4 serine/arginine repetitive matrix 4 2 2
MIRT581440 Rasa2 RAS p21 protein activator 2 2 2
MIRT582031 Pappa pregnancy-associated plasma protein A 2 2
MIRT582678 Lin28b lin-28 homolog B (C. elegans) 2 8
MIRT582831 Itih5 inter-alpha (globulin) inhibitor H5 2 2
MIRT583084 Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) 2 8
MIRT583879 Dusp18 dual specificity phosphatase 18 2 6
MIRT583900 Dstyk dual serine/threonine and tyrosine protein kinase 2 4
MIRT585980 Sfxn4 sideroflexin 4 2 4
MIRT590345 Cntn2 contactin 2 2 6
MIRT591765 Ppm1f protein phosphatase 1F (PP2C domain containing) 2 4
MIRT592938 Astn2 astrotactin 2 2 2
MIRT593579 Zfp709 zinc finger protein 709 2 2
MIRT593675 Slc5a3 solute carrier family 5 (inositol transporters), member 3 2 2
MIRT593750 Peg10 paternally expressed 10 2 2
MIRT593771 Nmnat3 nicotinamide nucleotide adenylyltransferase 3 2 2
MIRT593825 Krit1 KRIT1, ankyrin repeat containing 2 2
MIRT593859 Gm5460 predicted gene 5460 2 2
MIRT593871 Galnt10 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 2 2
MIRT593891 Elovl5 ELOVL family member 5, elongation of long chain fatty acids (yeast) 2 2
MIRT593935 Cxcl3 chemokine (C-X-C motif) ligand 3 2 2
MIRT593937 Cttnbp2nl CTTNBP2 N-terminal like 2 2
MIRT594001 Aptx aprataxin 2 2
MIRT594019 Tmem241 transmembrane protein 241 1 1
MIRT594730 Zfp931 zinc finger protein 931 2 2
MIRT594766 Tmlhe trimethyllysine hydroxylase, epsilon 2 2
MIRT594836 Opa3 optic atrophy 3 2 2
MIRT594873 Magt1 magnesium transporter 1 2 2
MIRT594927 Gm14326 predicted gene 14326 2 2
MIRT594941 Fosl2 fos-like antigen 2 2 2
MIRT594980 Eda2r ectodysplasin A2 receptor 2 2
MIRT595014 Cpd carboxypeptidase D 2 2
MIRT595076 Yae1d1 Yae1 domain containing 1 1 1
MIRT595132 Lrrc4c leucine rich repeat containing 4C 2 2
MIRT595148 Epha7 Eph receptor A7 2 2
MIRT595169 Zpbp zona pellucida binding protein 2 2
MIRT595180 Tsn translin 2 2
MIRT595185 Trim25 tripartite motif-containing 25 2 2
MIRT595187 Top2a topoisomerase (DNA) II alpha 2 2
MIRT595193 Tmtc3 transmembrane and tetratricopeptide repeat containing 3 2 2
MIRT595199 Tbc1d2b TBC1 domain family, member 2B 2 2
MIRT595204 St6gal1 beta galactoside alpha 2,6 sialyltransferase 1 2 2
MIRT595210 Snx27 sorting nexin family member 27 2 2
MIRT595226 Serinc1 serine incorporator 1 2 2
MIRT595229 Sema4d sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D 2 2
MIRT595232 Sec24a Sec24 related gene family, member A (S. cerevisiae) 2 2
MIRT595235 Scn3a sodium channel, voltage-gated, type III, alpha 2 2
MIRT595242 Rc3h1 RING CCCH (C3H) domains 1 2 2
MIRT595255 Ppm1k protein phosphatase 1K (PP2C domain containing) 2 2
MIRT595259 Plxna4 plexin A4 2 2
MIRT595264 Phf3 PHD finger protein 3 2 2
MIRT595271 Nxph1 neurexophilin 1 2 2
MIRT595273 Npas3 neuronal PAS domain protein 3 2 2
MIRT595298 Kcnv1 potassium channel, subfamily V, member 1 2 2
MIRT595317 Gna14 guanine nucleotide binding protein, alpha 14 2 2
MIRT595321 Gm10318 predicted gene 10318 2 2
MIRT595328 Galnt4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 2 2
MIRT595347 Frem2 Fras1 related extracellular matrix protein 2 2 2
MIRT595375 Epb4.1l4a erythrocyte membrane protein band 4.1 like 4a 2 2
MIRT595380 Dusp11 dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) 2 2
MIRT595383 Dek DEK oncogene (DNA binding) 2 2
MIRT595385 Dcun1d1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) 2 2
MIRT595391 Cetn3 centrin 3 2 2
MIRT595394 Cd8a CD8 antigen, alpha chain 2 2
MIRT595398 Asprv1 aspartic peptidase, retroviral-like 1 2 2
MIRT595403 Arid2 AT rich interactive domain 2 (ARID, RFX-like) 2 2
MIRT595414 Ankrd16 ankyrin repeat domain 16 2 2
MIRT595416 Anapc11 anaphase promoting complex subunit 11 2 2
MIRT595421 Akna AT-hook transcription factor 2 2
MIRT595438 9930013L23Rik cell migration inducing protein, hyaluronan binding 2 2
MIRT595441 9030617O03Rik D-glutamate cyclase 2 2
MIRT595459 Zswim6 zinc finger SWIM-type containing 6 2 2
MIRT595465 Tspan13 tetraspanin 13 2 2
MIRT595467 Tmem41b transmembrane protein 41B 2 2
MIRT595472 Sv2b synaptic vesicle glycoprotein 2 b 2 2
MIRT595475 Sgk3 serum/glucocorticoid regulated kinase 3 2 2
MIRT595480 Scaf11 SR-related CTD-associated factor 11 2 2
MIRT595485 Phox2b paired-like homeobox 2b 2 2
MIRT595490 Pgp phosphoglycolate phosphatase 2 2
MIRT595494 Nr1d2 nuclear receptor subfamily 1, group D, member 2 2 2
MIRT595511 Lrig1 leucine-rich repeats and immunoglobulin-like domains 1 2 2
MIRT595524 Grm2 glutamate receptor, metabotropic 2 2 2
MIRT595528 Foxn3 forkhead box N3 2 2
MIRT595536 Fign fidgetin 2 2
MIRT595546 Celf2 CUGBP, Elav-like family member 2 2 2
MIRT595553 Cd28 CD28 antigen 2 2
MIRT595840 Sumf1 sulfatase modifying factor 1 2 2
MIRT595953 Rab6a RAB6A, member RAS oncogene family 1 1
MIRT598445 Lix1 limb and CNS expressed 1 2 2
MIRT600750 Iws1 IWS1, SUPT6 interacting protein 2 2
MIRT601150 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 2 2
MIRT602248 Grm1 glutamate receptor, metabotropic 1 2 2
MIRT603333 Slc35d2 solute carrier family 35, member D2 2 2
MIRT603399 Serpina1a serine (or cysteine) peptidase inhibitor, clade A, member 1A 2 2
MIRT604755 Rad51d RAD51 paralog D 1 1
MIRT605865 Atpbd4 diphthamine biosynthesis 6 2 2
MIRT606713 0610030E20Rik RIKEN cDNA 0610030E20 gene 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-743b Propranolol approved 4946 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-743b-3p (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 down-regulated

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