miRTarBase - #MIRT578185


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pre-miRNA Information
pre-miRNA mmu-mir-92a-1   
Genomic Coordinates chr14: 115044427 - 115044506
Description Mus musculus miR-92a-1 stem-loop
Comment The predominant miR-92 form cloned by Landgraf et al. has a additional 3' U residue, which is compatible with this precursor sequence, but not with that of mir-92-2 (MIR:MI0000580) .
RNA Secondary Structure
pre-miRNA mmu-mir-92a-2   
Genomic Coordinates chrX: 52741838 - 52741928
Description Mus musculus miR-92a-2 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-92a-3p
Sequence 50| UAUUGCACUUGUCCCGGCCUG |70
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BE2BW7 miR-92a-3p Monitoring Biomarker (MOI) Clinical/Experimental Data Expression Increase Plasma Polymerase chain reaction
Gene Information
Gene Symbol Ncam2   
Synonyms Ncam-2, Ocam, RNCAM
Description neural cell adhesion molecule 2
Transcript NM_010954   
Other Transcripts NM_001113208   
Expression
Putative miRNA Targets on Ncam2
3'UTR of Ncam2
(miRNA target sites are highlighted)		 
>Ncam2|NM_010954|3'UTR
   1 TGTTCTATGTTCTATGGTACTCCCTGATCAGAGATAAAAGAGTAGGTACAGGCTAATCAAAAATTTTAATCAACATTTTC
  81 AGTCCAGTTCAATTTATGTTTACACAATTCGCCTTCCCCCTGAATTCAGTACTTTCTCTAATTTTATCTGTGTAATTTGG
 161 AAGAAAGCAATGCAGAAAGAAATTACAGAAGAAGTTTTTTCTTGCAAATACTTTTTCAGTAACAATATGTTTGACTCCAA
 241 ATCCATTACTTTGCAAAGTAGACTTTGTTACATTGAATTTAATTTCTATAGTGGGTCTTGCTTTCATTTCAGTATGCAAA
 321 TATCATTCAACATCCATCCATAAGTATAAAACAAGTAAATTTTTGTTTTACAAAATTGTGAATCACTTCTAACAAGTCTT
 401 TAATACTGTTAAAAGAAAATTTCTGGTTTTGTGAACATTTCTCGATTTTATAGTGCCTACCATTTATATAAATCATAAGC
 481 TTGATACCCTATTATATCAGGTACATCTATTTCTTGTTCTGCTTTCTGTAGTTTGAGATAAACACTCTCTGAAGAAACAA
 561 AATATAATCAAATTTCCTTTCATTTTTGAACATCCAATTAACATTTTCTTAAGTGAATATGCGTCTCTCCCATTATCGAT
 641 AATAAGTAAACTTAATCTCAGTACATTTAAATGCTACTAAGTTCAGTATGTTCACAGGAACCTTTGGCCAGTGGTGCAGA
 721 TCTAAATCTGTTCCAGGTATCTATTTCATTTTCACTACTGCTGACATTCCCTCTCTTTTGCAAGCATCAGAATACAAAGT
 801 GGCCATTTTAGGCATTACTTTAAAGGAGGTGGAAATGAGAGGGAAACAAGAGGCTGCTGTATTCAGATAAAAACAAAACA
 881 AAACAAAACAAAGCAAAGCAAAACAAAACCAAAGTAAAACACACAAACCCAAACTGAAACCAACCAACCAAACAAACAAA
 961 CAAAAAACTCAATTGGAAAAAATACAGGAAAAATTGTATGTCTTTCTCAAGTTTCGGTTATTCGTTAATGAGAGGAATGT
1041 GCTGTTAGCTTTGAATCACATCCTATGAAATAATGATTAGTATTTAAGGCAGTTAAGAAACCACACATGCACCAACAGAC
1121 ACACACACACACACACCAACACTCACACACATGCACATACAACCACACCATATAGACAAATATAACCATCTTTACTTGCT
1201 CAATCAAAAAATAAAAAACAAAAATTACAGCTTTTGAGATTCAACCCCTAAAATAAAGTATTCAGTTCTCTGAAAAAACT
1281 GTTCCTAATTTTGGGATACCCAAACATATTACTGCTTATGTGAGGTCCAAAGAGATTATATGTGCATTAGTTTCCAGCTT
1361 TATTTTAATTTATAATTTAAAACATCTAGCTTTCAATTATTTTTTTATCTTATAATTTGCAAAGTAACCTGCAAAATGAT
1441 TTGTTTAAAGACAATTTTTTTCCTCCTTGCTCCAACAACAAAAATTGGTAATTATTATAGTATGGAAACATAATTGACAT
1521 CTCGGGATATTTTTTTTTTGCAATATAACATGACCCATGCTTTATATATACATATATATGAATTAGATTTGAATTAGATT
1601 TCTTGATATTTGCCTGAATAAATGATATGATCATTACTGATGAGTAGAAAATATTGAGAATGATCATATTCAAGAAAAAC
1681 ACTTCTAAACGTCTTTCATCCCATCCATAATCCATGATGCTAAATATTTCCATGAAAATGTCATCCGTTATTTAACTTGA
1761 CTTGAAATCACAAATCATTTGGAACACAGGAAATGTTTGCTTAGTCAGTTACATTGATTGATTTTTTTAAGTTTATATCC
1841 CAGAACTACACATTACCAAGAGTGATACCAAAAATAGTCGGTGTCACGGTTACACAGAAATGTATCATTTTGGAAAACAC
1921 AAAATAACTGTATTTTACTAAAGGATCATAGAAATTACACTTACTTAAGGAAATGGCAAGGCTCTTGTTCGTTTTATTGT
2001 CCAAGTTACATTATTGTGTAAAGTCATAAGCCACTTCTCTTTACCCATTTGTGCCCTTAGGAACAGTCATTGATAATGTG
2081 CCCTGTGATAAGGAAATCATAAAAAAAAAATCCATATACTTCTTAATTTCTTCTCTATATTTTTCCTGTTTGCTTTTTAA
2161 ATAAATTCCAATTGTTGAAAGTATAGGCATATGTGTAATAAGGTGAGTTACTATTAACCATTATATGTAGGTAGTTAGAA
2241 AACTTTTGATCTAACAGTCTGTGTATCACAATTTATAGTGTTGAAATATTTCAGTAGATTTTGAACAGTGTAACAGTGAT
2321 GGAGCTTAGCGGATTTGCATGCTTTCCCATGTTATCTGTATGGATATGTGTCAAGTCAGGCTATCCTTTTAGTCATTTCA
2401 GAGTGTCTCCACTTTCCCTTCTTTTTTTTTTTTTTCCCATTTTTTATTAGGTATTTAACTCATTTACATTTCCAATGCTA
2481 TACCAAAAGTCC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guccggCCCUGUUCACGUUAu 5'
                || |:| |||:||| 
Target 5' agtataGGCATATGTGTAATa 3'
 2180 - 2200 139.00 -12.10
2 
miRNA  3' guccggcccuguucACGUUAu 5'
                        |||||| 
Target 5' ggatatttttttttTGCAATa 3'
 1525 - 1545 120.00 -6.70
3 
miRNA  3' guccggccCUGUUCACGUUAu 5'
                  | | ||| |||| 
Target 5' cattttcaGTCCAGTTCAATt 3'
 74 - 94 117.00 -6.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
24 mmu-miR-92a-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002971 Hipk3 homeodomain interacting protein kinase 3 2 1
MIRT002978 Mylip myosin regulatory light chain interacting protein 3 1
MIRT004280 Trp63 transformation related protein 63 4 1
MIRT007244 Map2k4 mitogen-activated protein kinase kinase 4 1 1
MIRT053813 Zfpm2 zinc finger protein, multitype 2 3 1
MIRT054699 Tbx3 T-box 3 3 2
MIRT054792 Shox2 short stature homeobox 2 1 1
MIRT578185 Ncam2 neural cell adhesion molecule 2 1 1
MIRT580680 Stk10 serine/threonine kinase 10 1 3
MIRT583677 Fam136a family with sequence similarity 136, member A 1 1
MIRT584193 Cpeb1 cytoplasmic polyadenylation element binding protein 1 1 1
MIRT587067 Gm5148 predicted gene 5148 1 2
MIRT589036 Rhbdl3 rhomboid, veinlet-like 3 (Drosophila) 1 1
MIRT595174 Zpbp zona pellucida binding protein 1 1
MIRT595805 Chm choroidermia (RAB escort protein 1) 1 1
MIRT595903 Cd69 CD69 antigen 1 1
MIRT596292 Tagap1 T cell activation GTPase activating protein 1 1 1
MIRT596301 Tagap T cell activation Rho GTPase activating protein 1 1
MIRT596475 Btg2 B cell translocation gene 2, anti-proliferative 1 1
MIRT598598 Il5ra interleukin 5 receptor, alpha 1 1
MIRT598840 Gm14420 zinc finger protein 970 1 1
MIRT601409 Uba1y ubiquitin-activating enzyme, Chr Y 1 1
MIRT606063 Zfp300 zinc finger protein 300 1 1
MIRT735310 PTEN phosphatase and tensin homolog 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-92a Vorinostat (SAHA) approved 5311 Microarray A549 human non-small cell lung cancer cells 19513533 2009 down-regulated
miR-92a 5-Fluorouracil approved 3385 Microarray HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Microarray HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Quantitative real-time PCR HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Quantitative real-time PCR HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a Oxaliplatin (L-OHP) approved 5310940 Microarray HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a Oxaliplatin (L-OHP) approved 5310940 Quantitative real-time PCR HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a 3,3'-diindolylmethane (BR-DIM) NULL 3071 Quantitative real-time PCR Prostatic cancer 22412975 2012 down-regulated
miR-92a Isoflavone NULL 72304 Quantitative real-time PCR Prostatic cancer 22412975 2012 down-regulated
miR-92a Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-92a Comfrey NULL 6440495 Microarray rat liver 21370286 2011 down-regulated
miR-92a Comfrey NULL 6440495 Quantitative real-time PCR rat liver 21370286 2011 down-regulated
miR-92a Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 down-regulated
miR-92a Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated

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