miRTarBase - #MIRT578529


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pre-miRNA Information
pre-miRNA mmu-mir-873a   
Genomic Coordinates chr4: 36668510 - 36668586
Description Mus musculus miR-873 stem-loop
Comment This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-873a-5p
Sequence 11| GCAGGAACUUGUGAGUCUCCU |31
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Ifit1   
Synonyms ISG56, Ifi56, P56
Description interferon-induced protein with tetratricopeptide repeats 1
Transcript NM_008331   
Expression
Putative miRNA Targets on Ifit1
3'UTR of Ifit1
(miRNA target sites are highlighted)		 
>Ifit1|NM_008331|3'UTR
   1 ATGCAGCTCACCTCTGTGACGTTAATATACTCACAACCAAGTGTTCCAATGCTCCTTCCTGATTTCTTTTCCCAGGTCTG
  81 TTTTGTTGTTGTTGTTGTTCGTTTTCTTTCTGAAATGCCAAGTAGCAAGGTACATTTTCTCCTGTGACTTCTTTCTGGTC
 161 TTTCTGACCCTGTCTAGCAGGCAATTCCATCCCAAGGATACATACTCACTTAAAGATCACAACTGATTCCTGCTGTTTTG
 241 GACTCCTGTGAGCTGTAGAATCACACAACCTTTTTCCTTATGTCTGTGCCTTTGGAACAGATATCTTCCACCCTTTGCTA
 321 ATGTCCTGGACAAAAAAGTCTTTGCCAAAGAAGGTATTTTTTTTTTAATGTCACCGGAAATGCTTATTACCATACAATTT
 401 CAGAGCAAAACAAATGAGCAAAGAAGAAAGTTTGAAAGCAAAAGAGGTCACACATTACTGGAAAGATACTAAGAACCAAG
 481 AAGACTTTTGGCTTACTAGAGAATTAAAATGAAAACCCTCCTTAGGTAGTAAAGTCTGAAAATTACATAAATAACTTCTT
 561 TCAATATTTATATGCTAAAAGTATTCATTGAAAGTGACCAAAATAGTTAAAGCCAAAACCAACAAAAAAAAGAAAGAAAG
 641 AAAGAAAGAGAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAAAAAGAAAAGAAAAGAAAAGAAAAAAAGA
 721 AGAAGAAAGAAAAAAAAAGAAAGCGACCCACAAATAACAAATTTGGTGTAGTCTATTAATGTTGTGCATTATAAAGGATG
 801 CACCTCTATGTTTGAGCAGTTTCTTGGTTATCTTTTATCCTCAGCTTGATATATCCTGGGACATCTGAAAAGAGCTGCTT
 881 GCACCACACTAGCTTGCAGGCATATTGTGGCACTTTTATCCTGGATTCCTGACTGTTGTCGTATGACACAGTCAACTGTG
 961 AGTGCTTCCATCCCTACGCAGGTAGTCTTGGCCTATATATGAAATCTTGCTGTGCATGTGCCAATGAATGAACCAGCAAG
1041 CAGAATTACTCCATGATTCCTACCCAGTTTCCTGCCTTGACTTTTCTGCCCTGAGTTTCCTCCCTGATGGATTATGATCT
1121 CTAAGTGTAAGTCAAATAAACATATTCCCCTC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uccUCUGAGUGUU--C-AAGGACg 5'
             ||| ||||||  | |||||| 
Target 5' taaAGA-TCACAACTGATTCCTGc 3'
 211 - 233 135.00 -16.00
2 
miRNA  3' uccuCUGAG--U--GUUCAAGGACg 5'
              ||:||  |  ||: |||||| 
Target 5' ccatGATTCCTACCCAGTTTCCTGc 3'
 1051 - 1075 125.00 -13.80
3 
miRNA  3' uccUCUGAGUGUU------CAAGGacg 5'
             | ||||||||      |||||   
Target 5' aatATACTCACAACCAAGTGTTCCaat 3'
 24 - 50 124.00 -12.02
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
17 mmu-miR-873a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT438380 Tnfaip3 tumor necrosis factor, alpha-induced protein 3 3 1
MIRT577099 Wdr26 WD repeat domain 26 1 4
MIRT577463 Trmt61a tRNA methyltransferase 61A 1 3
MIRT577540 Thnsl1 threonine synthase-like 1 (bacterial) 1 1
MIRT578417 Kcnj11 potassium inwardly rectifying channel, subfamily J, member 11 1 1
MIRT578529 Ifit1 interferon-induced protein with tetratricopeptide repeats 1 1 1
MIRT581343 Rgs17 regulator of G-protein signaling 17 1 1
MIRT584008 Dcbld2 discoidin, CUB and LCCL domain containing 2 1 1
MIRT586514 Ndufaf4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 1 1
MIRT586607 Mob3c MOB kinase activator 3C 1 1
MIRT589481 N4bp2l2 NEDD4 binding protein 2-like 2 1 2
MIRT591646 Tmx4 thioredoxin-related transmembrane protein 4 1 2
MIRT593307 Stx16 syntaxin 16 1 1
MIRT598176 Mrpl45 mitochondrial ribosomal protein L45 1 1
MIRT602468 Dnajc18 DnaJ heat shock protein family (Hsp40) member C18 1 1
MIRT603743 Mrpl41 mitochondrial ribosomal protein L41 1 1
MIRT604791 Pik3r1 phosphoinositide-3-kinase regulatory subunit 1 1 1

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