miRTarBase - #MIRT578803


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pre-miRNA Information
pre-miRNA mmu-mir-1912   
Genomic Coordinates chrX: 147009441 - 147009527
Description Mus musculus miR-1912 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-1912-3p
Sequence 53| CACAGAACAUGCAGUGAGAACU |74
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Foxl1   
Synonyms FREAC7, Fkh6, fkh-6
Description forkhead box L1
Transcript NM_008024   
Expression
Putative miRNA Targets on Foxl1
3'UTR of Foxl1
(miRNA target sites are highlighted)		 
>Foxl1|NM_008024|3'UTR
   1 GGCGGATTAGCGGCTGCTTCCCTGCCTCGCGATCCCCCCACCTCCAGTGAGCCAATGCCTGTGGGCGAAGGAGTCGCCCC
  81 GGTCTCTGCCCTGGCAGAGACTGAGGGTTTGGAGCAGCGTACCCGTGCCGGCCAAAGTCGTGCACTGAGGGGCCTCGTCC
 161 ATCTGCTTTCTCCGAAGATGACTTAACCAGCCACCGGAAGCTTTGCAGATTTAAAAGACTGGTCCAGTAGAGGACATGGT
 241 CGCCGCACACTCAGCCCTCACGGTTCGTCTCCAGCAGAAGCCCCTCGTAGGCTTTGGCGGCGTTGTACAGTGACCACCAC
 321 AAGTCCCCACTCGGACCTGAGTCTCCTGCTGACCCTGAGGGCAGGACTTAGAGATATAGCGAACAGCTCTATGTGGAGGT
 401 TCAACTGGATCATGGAAGGCTCAGCGGGCTCCGTGTGGCATAGGCCTTGGCTTTGATCTCATTGGATGTGGGGAGCTTGC
 481 AGCCAGGATGCTGGGGATGATCCGTGGTCCGCAGGCTCTGGGAAAGATGTAGGCCTGGTTAACGTTTACAACTAAGGTTG
 561 AACAAAAGCAGGCTCACCGGAAATGCTCAATATGAATAGATAAACCTTCTTCCAGACAGGGAAAGGCTCATTTATTCTTG
 641 GCTTATAAACCAGGCTCCCCTTTGCTATAGCTAGTAGCTTCTTTTAAGGAGCCCCGTGTGTTTGGCACGTACTTTGGCTT
 721 TACAGTGGCACACCTGCCGCATTCCACAGCATAGTCCTCTGTTCACTTCTGCTTTTCTTTTCTTTTTTTTTTTTTGTGTG
 801 GGGGGGAGGGGTTCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTGC
 881 CTCTGCCTCCCGAGAGCTGGGATTAAAGGCGTACTCCACCATGCCTGGCCTGCTTTTCTTTTTAAGGCACCAAAAAATTT
 961 CAGGGGACACAGAAGGGTGTGTGATCGGAGGGATTATTATGACTCAAGGGCGGGTTTTTTTTTTAACTTCTGTAGCTTTT
1041 TAAGATCTAAGTCCCCCCCCCATCTCTCTCCCCCCCCCCACTCACACTTTCCAGAAACACACACACAGTGAAGGACTACA
1121 TGTAATCAGAGAGGCTGGGGGAGCATACTACTCCTGAGTGTGGGAGATAGATAGCTTCCAGGAGTGACAGGGACTCTCTT
1201 CAGACAAAGCCTGTGGTACCTCAAGTCTGTGTTCCCTTTATATCCCTCCAGGGAGATCTAGATATTGACATTACATAAAC
1281 CCATAAGAGGTGACCAGAGAGCGGGGTTGAGGGGGAGGGGGTGGGGAGATCAACAAAGTGCTGACTAAGGCAACTAAGTT
1361 GGTTACCGTGCAGTGCAGTCCTGCTTCCAAATTTGGGGGAAACTCTCAGGCCTTTGGGGTGCCCATGTTTAAGCTTGCTG
1441 GTGGGCTCTGCAACTCAGCTTTCAGAGGAAGGAAAGTGCTGGTCCTTGATGTAAAAAAAAGGACCAAAAATTAAGAATTC
1521 TAGGGCATAGGTCCACAGTGATGTCTCACAACAACTAAGGTATGCCGTGCATCACACCTGTAATCTCAGTCCTTGCAAGG
1601 CTGAGGCAGGGGGATTGCTGTGAGTTTAAGGATAGCTTGTGGCACAGAGTTAGACATGTCTCAAACTAAGGG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ucAAGAGUGACGUA-CA--AGACAc 5'
            ||| ||| |||| ||  ||||| 
Target 5' caTTC-CACAGCATAGTCCTCTGTt 3'
 740 - 763 131.00 -11.70
2 
miRNA  3' ucAAGAGUGACGUACAAGACAc 5'
            ||:|: :| :|  |||||| 
Target 5' ggTTTTTTTTTTAACTTCTGTa 3'
 1013 - 1034 128.00 -6.59
3 
miRNA  3' ucAAGAGUGACGUACAAGACAc 5'
            ||||: :| : | ||:||| 
Target 5' ttTTCTT-TTTTTTTTTTTGTg 3'
 778 - 798 111.00 -6.76
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
43 mmu-miR-1912-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577511 Tmem100 transmembrane protein 100 1 1
MIRT577755 Shh sonic hedgehog 1 1
MIRT578803 Foxl1 forkhead box L1 1 1
MIRT579268 Btnl9 butyrophilin-like 9 1 1
MIRT579274 Btn1a1 butyrophilin, subfamily 1, member A1 1 3
MIRT579457 Acsm2 acyl-CoA synthetase medium-chain family member 2 1 1
MIRT581022 Sirpa signal-regulatory protein alpha 1 1
MIRT581556 Ptchd1 patched domain containing 1 1 2
MIRT581946 Pex12 peroxisomal biogenesis factor 12 1 1
MIRT582622 Lrat lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) 1 1
MIRT583140 Vimp selenoprotein S 1 1
MIRT583710 Ezr ezrin 1 1
MIRT584224 Col24a1 collagen, type XXIV, alpha 1 1 1
MIRT584232 Cntn3 contactin 3 1 1
MIRT584483 Caprin1 cell cycle associated protein 1 1 1
MIRT586095 Reep5 receptor accessory protein 5 1 1
MIRT586114 Rdh1 retinol dehydrogenase 1 (all trans) 1 1
MIRT586222 Pter phosphotriesterase related 1 1
MIRT586885 Htr1a 5-hydroxytryptamine (serotonin) receptor 1A 1 1
MIRT587345 Efcab4a calcium release activated channel regulator 2B 1 2
MIRT587504 D3Ertd254e DNA segment, Chr 3, ERATO Doi 254, expressed 1 1
MIRT587620 Cmklr1 chemokine-like receptor 1 1 4
MIRT588930 Sgms1 sphingomyelin synthase 1 1 1
MIRT589357 Osbpl8 oxysterol binding protein-like 8 1 2
MIRT589494 Mzt1 mitotic spindle organizing protein 1 1 2
MIRT589912 Gng2 guanine nucleotide binding protein (G protein), gamma 2 1 2
MIRT590951 Stxbp4 syntaxin binding protein 4 1 1
MIRT592096 Slc25a42 solute carrier family 25, member 42 1 1
MIRT594345 Mfsd11 major facilitator superfamily domain containing 11 1 1
MIRT595009 Creb5 cAMP responsive element binding protein 5 1 1
MIRT596577 Zfp790 zinc finger protein 790 1 1
MIRT596663 Zfp322a zinc finger protein 322A 1 1
MIRT597521 Rbak RB-associated KRAB zinc finger 1 1
MIRT598049 Nip7 NIP7, nucleolar pre-rRNA processing protein 1 1
MIRT599553 C3ar1 complement component 3a receptor 1 1 1
MIRT600483 Rasal2 RAS protein activator like 2 1 1
MIRT601104 Btrc beta-transducin repeat containing protein 1 1
MIRT601424 Tulp3 tubby-like protein 3 1 1
MIRT601924 Onecut3 one cut domain, family member 3 1 1
MIRT601995 Mrpl15 mitochondrial ribosomal protein L15 1 1
MIRT603982 Arl14epl ADP-ribosylation factor-like 14 effector protein-like 1 1
MIRT604409 Ado 2-aminoethanethiol (cysteamine) dioxygenase 1 1
MIRT606424 Fam96a family with sequence similarity 96, member A 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-1912 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated

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