miRTarBase - #MIRT579903


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pre-miRNA Information
pre-miRNA mmu-mir-302a   
Genomic Coordinates chr3: 127545496 - 127545564
Description Mus musculus miR-302a stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-302a-3p
Sequence 44| UAAGUGCUUCCAUGUUUUGGUGA |66
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Zbtb41   
Synonyms 8430415N23Rik, 9430031N01, 9830132G07Rik, AI316857
Description zinc finger and BTB domain containing 41
Transcript NM_172643   
Expression
Putative miRNA Targets on Zbtb41
3'UTR of Zbtb41
(miRNA target sites are highlighted)		 
>Zbtb41|NM_172643|3'UTR
   1 GTAACCATTCCATAGGCCTTTTAATGCTGTAAGCTGACAGCCAGAAGCTAATGGCTTTTGAATTAGTAGGGCTGCTATTT
  81 TTTCCCCCATCTTTTTCCCCTAATTTCTCAAGTCTTCAGAACAGCTTCAAATCAAGAAAAGTTTATTTCTATTGACTGAA
 161 TACTTGGACACATTTTGACATATTTGAAGTATGATTTTTTAAAAAAAAATCATTAGTACTAATTTTGAAATTTTTAAATT
 241 TCAGATTATTAGCTGCTGACAATGGTGGAGGTATTTTTTTTAATCATCAGTGGAATTTTTTGTTTGTTTGTTTTTTTTGA
 321 GACAGGGTCTTAGTTTGTAGCCCAGGTAGCCTCAATTTGTGATAGACTTTCTGCCTTAGCCAACCAGTTGCTAGGATTAC
 401 AGGTGTGAGCCCCAAGCCAAGGTTTTACTTTTAATCATCTGTTTTGTCCGTTTCCCATATTGCTATGAAGTTTTAAGAAC
 481 CATGTAGCCTATAGGAAGGAATGTAAACATATGTCAATATTGGCAAAATTTTAATACTAATTAAATATTTTGTTCAGATT
 561 TTCAAAGTTGAGTTTAGAAACTTATTGAAATTTCTTACATATTTTTGTAAGAAAATCTTGATACTTTGAGGTTGTCATCT
 641 ATCCATTCTTTTTGTCAGTATATTTGGGTTTTTCACAAGCCAAAATCCAAGAGCTCACAATTACAGGATTAGCATGCTAA
 721 ATGAAATATAAGGTTGTTATTTTAATCCAGATGATTGGACCTGTACTCAACTATGATAAGTAATAAATACAAACTTAGCT
 801 CTAGACATTAAATTATCTAATTGAGTATATCGTAATGAAAAACTGTCAGTTTCACCTACATATCATCAATGTACATTGGC
 881 ATTTTGTATTTGGGCACTTCCTCCTCGTTCCCAGTATGTGTCAGTATCTACAGATGAGTAAGGAAGGAAGGGACAAGTGT
 961 AACTAAAGTGATGGCAGTTCAGTTACACAATGGAGTTAGCATTTCATTTACCACATTTGGCTGACTGAAGCAGGGAAACA
1041 GAACCTAGATATTTTACGTGTAGTTATCAGAAAAGGTGCCAAACCCATTCTCAAATCCGCCCTAACTTAACAGGATGCTC
1121 TTCAGCTGAGTGCACTACTGTTGGTGTATAGATTGACACTTAAACCAGATCTTTCTTTTTGCTTCTGTATGAGATTTGTC
1201 TCTATTCTTCATTGTTTCTGGATGAGGCAGTGGTTGAAGTCTTGGTGAAAGAAAATAGGATTTACAGCAGCGCTTCACAG
1281 ATTAAGTTTAAGTGAAGGACAAAATCTTCCAGAATTACGAAATATCTTTAGAGATGTGCACTTTGCATTATTTTCAACTC
1361 AATTTCATCTTAATATAGGATGAGTTTTTCCTTGTGGGGTTGCTGATGCTTAAGACAGGTAGGGTTGGCTCACTGTTAAC
1441 ATGCAGTTTGTATGGAAACTACTGGAAAAATAGCAGTTGTCAAAACTTTTCTATTTCAATTTGTTTACACTCCTAATTGC
1521 TTTTAGCACTTTTGTGTGTGCGTGTGTGCGTGTATGTGTGTGTGTGTGTAAACTGTAACGTTTTGCTTGGCCCTTGTGGG
1601 TTTTGAAAACATATCACAAATAAAATAATAAACTTCTAGTGAATAAAATGTAATCAAAAAGGAGGAAACTGTTTAAGCAC
1681 TTAAGAAATGAAGGTACTTTATAGGAACAGTTGCCATATTAATGTTCAAAGAGCATGTTGCAAATTGAGGCATTAACCAA
1761 AGTAGCTAATATAAATAATAAAGATAACCTGAGTTTTGCCTCACTTATCAAAGTTTGGTTGTTGTAATTGTCTTTGGACT
1841 AGTGAACTTAATTTTGTATTATTATTGCTAAAGTCTTCTTACTTAGTGTGGAAGTGTTCTGTGGGCACTGATAACTGAGT
1921 AGAGTCTGAGCACTATGAGTCCTGGACTTATAAATCATTGTCAAGAGTACAGATTTGTGATTTCTATCCAAAGGCAGAGA
2001 AGATTTCTAACTATCTTACTGAAATGAAAACATCATACTTAGAAATGTACTTTGCTTTAGAGATAAGCTAATGTAGAAAA
2081 TTTTGGGTTTATCAGAAAAGGCAAATGTTATATATGTGTTTATGTGCATGATGAATTGTATATTCTGACAGAAAAGGAGG
2161 ATACAGCATTTTCACAGTGGAAATATTACCTTGGGTTTTGAAGGTATTTAAGGTTCTTTCAATAATGATATTCTAACACT
2241 TTAAATATATGACTTAATTTTAGTAATTGGTTTACATAGGACTTTTGAAGAATATAGCTCTTATGTAAAGTGGCATACAA
2321 TTTTATAATTGTACTTAGCTATTATTGTAGAAAAGCTGAAATAGTGTCTGTTATTTAAACCATTTTCATGTTATCTCATT
2401 TGAACCTAGTAAGCAATTTTGAAATAGAAAGGAAATGATGTATTTCCATTTTAGAGAGATACTGAGAGTCATTTAGAGTC
2481 ATTTGACTCTACTCTCATGCCAGAACTGCCTTGAGTGGAAGGAGGAGACACATGGCATTAAAAAGGCTTACTATGTGCAG
2561 GACTGTGTTCTCCAGTTCCTAACGCAGTCTGCAGGTAGACAGTAGAATATTTAGGAAGAAATGAGGAAACCCATAATAAT
2641 GAAAAGATTACTGTTCCCTCTAGTTTCCAAAAATGTAAGAACATGGATTTGAGAAAACTGAATGTATAAATATTTTAAGT
2721 TAGCACAAAAATTATAAAATAGACCCTACTCTTCTTAGTTGTCCTTTTTATGACTCTAAAATCATTATTTTGTAACTATG
2801 GTTAAAATTTTGAATCTCACACGTACGTTTAGCCTCATTGTGAATATTTTGTGGGTATAATTTATTGTTTTAAATTAATT
2881 TTGGTAGATACGTTAGCACTTAATCTGTAAATTGAATAAGTTATTGAGTGCAGAAATTTATTAACTTACTGTCACTGACT
2961 TAATGCACCAAAGATTTTGAGTAGGTATTGAGTAGTTGAAAAGAATTTAAATTCTTTGTTGTTTGACTATATATTTGTCC
3041 AAAGAAGCACAGCTGACGTCCTTGGATACTGAATTTTAAAAATTGTTAGTTTGAATACTAAGATTAAGTATTAAATGTTT
3121 GTGTTTATGTGTATAAAATAAGTAGAGGAAAGTACTCTGGGAACATTCATACTGACCAACCAGTTACAAGTGAGAATACT
3201 CATTTCTCTCAGTTTAGTTGAATGCAGTTTTTTTTTTAAATGAACACTATTCAATAATCTTATATTTTACACTACATTAT
3281 AAAAATAATTTCTGCCTTAGAGGTTTTTTGTTTGTTTGTTTTTTAGATCAAATCGAGGTTATTTTTATAAGAAATCCCTT
3361 TGGTTTTGACAGCCTAAAATACTACGTAACCATAGGGTTTCTTCCTCATGCCAGCAAGATACCTTTAAGCTATTATAAAA
3441 GTAGTAAAGTTTGCAGGCTTAATCTAGAGCCTTTCTGCTGGGCAAAGAGGAATAAAAATACCATTTAAACTGGTGCTTTA
3521 ATAAACTGACTGCCTTTTAATGATGGTAGCATAAAAGAAGTACACTGCTTTCTTTCAACCTTAAAAAGTGTCAAAAAACA
3601 CCTTTTTGGAGTGACTGTAAAGTATTAAGGGCTAATCTGTTCCACCTATTGCTTTCTCACTCAATCACAAGTTACCTAAA
3681 CACAACTAACCTGCTGTGTCTATGAGCTTGAGTGTTCTTCCTACAATATCACTCTAATTTATTATTTATCAAGAGGTTTG
3761 AACATTAGTTAAGAGTGTCCTTTTAGACCATCTTTTCTGAATTTGAATACTTTGTACTTTTTCAGAGATGTGTAGTTGTG
3841 TAGCATTTTCTCTGTTGCCTAGATTGTACTGGTAAATAAATGACTTGTTCATTTTGTTTTATTTTTTGGTTCTGAGTCTT
3921 AATATCCCTGTTGCCTGCTGGCTGGGCAATAGCCTTTTACATGTTATATTAACTTAGGAAAATTGGCATTTCGCAGTAGT
4001 GTGGACATCGGTCTTTATTATAATTTACATGTCTTCTTTTAAGTTTGTACTGTGGCCTTTATAGCTATGGAGTCACTCCT
4081 CAGCCTGTCTAGATGTTGAGCAGAATCGGAGAGAAGGAATTGTATGTCTGCTCCTCTTGTTACTCTTTCTCTCTGACTGC
4161 TCTCCCCTTAAGGAACTTGTTACCAGGAACACACAGCTCTGCACCGGAGACTTAGTTCACTTTCCTTGAACTGAACGTGG
4241 TTGGTGGTGTAAGAAGAGCTGCTGTTAGTGAGCGGCGGCATCCTAGTCAACATTGAACTTGAGTTGATGACTCAGGAAAA
4321 AGCACGACTTTTCAACAGTCATGTTTGGACATATTTAGTGCCAAATTTCAAAAGACTGAAAATGAATAATTTAATAAGGA
4401 ATAAGTACTGTTTCTTAATTTTTAAGTGGAATTATGTTAAATGTTACCTACTATTAGTGTGAGTAATGAATTGAGAGTAA
4481 TTTTCTACACAGCTTATAGGAACTTGACAATTCTGCATATCCCACTCATTTCTTTTGCCTGTTTTCCTTTTAGTAATCTC
4561 TGTGTGTTCTTTTTTGGTGTCTTTCCATGTTTCTTATAACATTTTTACTTACAATACTTTCCCTTGTGTTATCAGCATGT
4641 CTTGCGCTTCCTCTTTGGAAGTGGGTTATTATTACAAAAGCAATAGGATTAGATTGATTGTGTGGGAGATGAGAAGACGG
4721 TGATTTGGCAAGGAGGGTGTGCTTACGTTCGACTTATTCGTGTTTTAACTTACTGTATATTGGTCTTAAGTCTGTGATCT
4801 TTGAAAGATCCCCAAGCCACTTATTTAAAGAGAATTTAAAGATGTTATTTGGAGAACAAATGTTACTTAAAGATGAAAAC
4881 CTTCTGAATTCTCAAGTAAAGCTAAGCAATTTCAAAATTTGCATTACTGCCGGAGATTCTGAAACGTCTCTTGAATGAAG
4961 AGCATGTGTACATCTCATTGTCACTATGCCTCTCTGTTTGATATTCCTGCTTGTATAAAGTTGAATAATGTACTGTGGTG
5041 TGATTACTGAACTTTATCCTTTTAACAATCCGACTTTTCTCCTTGTAACTATTAACAGTTGATGAGGGTGATTGCCAAAA
5121 GCTAGGTGTTTAGCTGCTCTGTGTTCTGTCATAACTGTTCTATTTAAGGGAGAAATGGTATATATTGAAATAGAAATGCT
5201 TTTGTTTTCTGTCTCTAATCAGAATCTTTGTTTTTAATTAAGGACAGTTTTAAAAGCAATTACAAAGGAAAATCTAAAAT
5281 ATCAAAATTCATTATTTTTCACTGTATGACAAATGTCTTAAGTTTAGTAGATATTTTGTTTGTCTCGTGTTCCTTAAATA
5361 TAAATGTATAGCAAAAATTCAGTTCCTTGTGAGGAGTAGATGAGGTCTTGCATATTAAATTAGTTCCTTATGTTATTTTG
5441 ATATTTAAAATAAACAGTTCTTTGGTCCTAGTAAAGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aguggUUUUGUACCUUCGUGAAu 5'
               ||| ::|  |||||||| 
Target 5' ggaggAAACTGTTTAAGCACTTa 3'
 1661 - 1683 158.00 -11.70
2 
miRNA  3' agugguUUUGUACCUUCGUGAAu 5'
                |:|:| |  ||||||| 
Target 5' tttggtAGATACGTTAGCACTTa 3'
 2880 - 2902 153.00 -11.60
3 
miRNA  3' aguGGUUUUGU-ACCUUCGUGAAu 5'
             || || :: |   ||||||| 
Target 5' actCCTAATTGCTTTTAGCACTTt 3'
 1509 - 1532 144.00 -9.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' agugguuuuguaccuUCGUGAAu 5'
                         ||||||| 
Target 5' ----------cuuuuAGCACUUu 3'
 		1 - 13
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
73 mmu-miR-302a-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT054363 Lats2 large tumor suppressor 2 4 1
MIRT054577 Ccnd1 cyclin D1 4 1
MIRT578213 Myl6b myosin, light polypeptide 6B 1 1
MIRT579773 Zfp568 zinc finger protein 568 1 1
MIRT579903 Zbtb41 zinc finger and BTB domain containing 41 1 1
MIRT582206 Nfatc3 nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 1 1
MIRT589603 Luc7l2 LUC7-like 2 (S. cerevisiae) 1 4
MIRT592531 Mup7 major urinary protein 7 1 1
MIRT592581 Mup13 major urinary protein 13 1 1
MIRT595334 Galnt4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 1 1
MIRT596464 Fam168b family with sequence similarity 168, member B 1 1
MIRT596522 Zfp882 zinc finger protein 882 1 1
MIRT596544 Zfp866 zinc finger protein 866 1 1
MIRT596766 Usp45 ubiquitin specific petidase 45 1 1
MIRT596795 Ubxn2a UBX domain protein 2A 1 1
MIRT596925 Tprkb Tp53rk binding protein 1 1
MIRT597148 Stxbp4 syntaxin binding protein 4 1 1
MIRT597693 Ppp2r3d protein phosphatase 2 (formerly 2A), regulatory subunit B'', delta 1 1
MIRT598003 Npy1r neuropeptide Y receptor Y1 1 1
MIRT598028 Nol10 nucleolar protein 10 1 1
MIRT598114 Ncl nucleolin 1 1
MIRT598150 Mtif2 mitochondrial translational initiation factor 2 1 1
MIRT598303 Mc1r melanocortin 1 receptor 1 1
MIRT598345 Mau2 MAU2 sister chromatid cohesion factor 1 1
MIRT598390 Magt1 magnesium transporter 1 1 1
MIRT598435 Lpl lipoprotein lipase 1 1
MIRT598657 Hpgds hematopoietic prostaglandin D synthase 1 1
MIRT598871 Glyctk glycerate kinase 1 1
MIRT598885 Glrx2 glutaredoxin 2 (thioltransferase) 1 1
MIRT599161 Diap2 diaphanous related formin 2 1 1
MIRT599255 Tmem245 transmembrane protein 245 1 1
MIRT599414 Chek2 checkpoint kinase 2 1 1
MIRT599539 Car5b carbonic anhydrase 5b, mitochondrial 1 1
MIRT599747 Anapc16 anaphase promoting complex subunit 16 1 1
MIRT599850 Adarb1 adenosine deaminase, RNA-specific, B1 1 1
MIRT599896 Acat1 acetyl-Coenzyme A acetyltransferase 1 1 1
MIRT599919 A830018L16Rik RIKEN cDNA A830018L16 gene 1 1
MIRT600228 Trim56 tripartite motif-containing 56 1 1
MIRT600269 Tns4 tensin 4 1 1
MIRT600435 Samd8 sterile alpha motif domain containing 8 1 1
MIRT600863 Gad2 glutamic acid decarboxylase 2 1 1
MIRT600876 Gabpb2 GA repeat binding protein, beta 2 1 1
MIRT600951 Elf1 E74-like factor 1 1 1
MIRT601038 Cntn2 contactin 2 1 1
MIRT601252 Abl2 v-abl Abelson murine leukemia viral oncogene 2 (arg, Abelson-related gene) 1 1
MIRT601369 Ybey ybeY metallopeptidase 1 1
MIRT601493 Tmlhe trimethyllysine hydroxylase, epsilon 1 1
MIRT601936 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 1
MIRT601974 Nav1 neuron navigator 1 1 1
MIRT602014 Mpp7 membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) 1 1
MIRT602186 Hyal1 hyaluronoglucosaminidase 1 1 1
MIRT602216 Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 1 1
MIRT602228 H2-T3 histocompatibility 2, T region locus 3 1 1
MIRT602245 Grm1 glutamate receptor, metabotropic 1 1 1
MIRT602367 Fbxl3 F-box and leucine-rich repeat protein 3 1 1
MIRT602425 Ercc4 excision repair cross-complementing rodent repair deficiency, complementation group 4 1 1
MIRT602490 Dkc1 dyskeratosis congenita 1, dyskerin 1 1
MIRT602596 Chrnb4 cholinergic receptor, nicotinic, beta polypeptide 4 1 1
MIRT602684 Camk4 calcium/calmodulin-dependent protein kinase IV 1 1
MIRT602761 Asxl2 additional sex combs like 2 (Drosophila) 1 1
MIRT603033 Chic1 cysteine-rich hydrophobic domain 1 1 1
MIRT603526 Pwwp2a PWWP domain containing 2A 1 1
MIRT603813 Kif5b kinesin family member 5B 1 1
MIRT603883 Il10rb interleukin 10 receptor, beta 1 1
MIRT604185 Cxcl11 chemokine (C-X-C motif) ligand 11 1 1
MIRT605161 C030039L03Rik zinc finger protein 607B 1 1
MIRT605480 Slc6a2 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 1 1
MIRT605613 Mpv17 MpV17 mitochondrial inner membrane protein 1 1
MIRT605789 Ddx11 DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 1 1
MIRT605806 Emc8 ER membrane protein complex subunit 8 1 1
MIRT605843 Cd300a CD300A molecule 1 1
MIRT731069 Nr2f2 nuclear receptor subfamily 2, group F, member 2 3 1
MIRT734874 FOXO1 forkhead box O1 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-302a Catechin approved 9064 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-302a Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated

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