miRTarBase - #MIRT580097


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pre-miRNA Information
pre-miRNA mmu-mir-452   
Genomic Coordinates chrX: 72262224 - 72262308
Synonyms Mirn452, mmu-mir-452, Mir452
Description Mus musculus miR-452 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-452-3p
Sequence 53| UCAGUCUCAUCUGCAAAGAGGU |74
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Usp54   
Synonyms 4930429G18Rik, AI115571, C030002J06Rik
Description ubiquitin specific peptidase 54
Transcript NM_030180   
Expression
Putative miRNA Targets on Usp54
3'UTR of Usp54
(miRNA target sites are highlighted)		 
>Usp54|NM_030180|3'UTR
   1 GGGCCACCCTAACCTGCCCTGGAGCTACACCTTCATCCATAAAACTGTACGGCGGCGCAGTGACACCTTGTTTCCTGCCA
  81 GTTATGCCAGTCTCATCTCAACAGACTTGGCCATTTCATTCCAGGCACCATATGCCTGTAATCCCAGTAGTTGGGAGGTA
 161 GAGATTGGGGAATCAGCATTTCAAGGCCAGAGACTGGGGTAAAAAGAGACTGTTTGTTGTTGTTGTTGTTTTGTTTTAAA
 241 GAACAGAACCACAACAACAAAGAAGACATTGATTTTTTTTTCCCCTAAGGGTGTTTGCTGCTTTGAGAGGGAATATTTGA
 321 AATTCCAGCTTTAGAGTTGGGGATGTAGCTCAATTGGTAGCGTGTTTTTGTAACATGCACATCAGCCATGGTTCTATCCT
 401 TAGAAGTGTGTGAAACTGACCATGGTGGTGCACAACTGTCATCCTAGCACTTGGGAGACAGAGGGAGGAGGATCAGAAGT
 481 TCAGGGTTATCCTTGGCTACATAACAAGTTCTAGACTAGTCTGGACTACTTTAGTTCTGGTTCTTTTGGCCCCATCTCCA
 561 TCAGAGCCCTCATCTTCTGGTCCTACTATACTCCCAACTTACACTCTTTGTTTATCCACCTGTCCATATTACTTAACATT
 641 CTTTCTTCCCTATTTCGTATCAATGGAAAAGGGGTATTTATCCCCATAGTCAGGATTCTGACCACGTCCTTAAGGGAAGC
 721 CCAACTGCCCAGGTATTATGGCCTGTCAGCCTTCCTTGTGACTCAGACTTTAGGCCTAGAATGTGGAGCTGCCAATACAC
 801 TGTTTGCCCTTCTGAACAGATGGAGTTGGGCACACTAACACCCCTCCTGTCCTTAGCAGCAGAGCCATTACTACCACTGC
 881 TCACTCACCCTTGTAAACTCTCAAGAGTCAGCTAAAGTATTTTAGGGCAAAGCATACTGGTTTTGTAAAGTATTTTTCAT
 961 TAATAAATCTATAAGATAGTTGTATTTAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uggagAAACGUCU--ACUCUGACu 5'
               |||  ||:   ||||||| 
Target 5' cagcaTTTCAAGGCCAGAGACTGg 3'
 174 - 197 147.00 -13.20
2 
miRNA  3' uggagAAACGUCUA-CUCUGACu 5'
               || | ||:| ||||:|| 
Target 5' agtagTTGGGAGGTAGAGATTGg 3'
 146 - 168 141.00 -14.30
3 
miRNA  3' uggaGAAACGUC---UACUCUGACu 5'
              ||| | ||   :||| |||| 
Target 5' tatcCTTAGAAGTGTGTGAAACTGa 3'
 395 - 419 126.00 -11.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ugGAGAAACGUCUACUCUGACu 5'
            || ||||: | ||  ::|| 
Target 5' --CUGUUUGUUGUUGUUGUUGu 3'
 		1 - 20
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM4751762
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 3
Location of target site NM_030180 | 3UTR | AAGGCCAGAGACUGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_030180 | 3UTR | CUGUUUGUUGUUGUUGUUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM1385343
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_DM_Ago2_CLIP-Seq_myotubes
Location of target site NM_030180 | 3UTR | CUGUUUGUUGUUGUUGUUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
26 mmu-miR-452-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT429003 Nfib nuclear factor I/B 2 2
MIRT577016 Vmp1 vacuole membrane protein 1 2 8
MIRT580097 Usp54 ubiquitin specific peptidase 54 2 2
MIRT581145 Senp1 SUMO1/sentrin specific peptidase 1 2 2
MIRT581215 Samd4 sterile alpha motif domain containing 4 2 2
MIRT582710 Lcorl ligand dependent nuclear receptor corepressor-like 2 6
MIRT583742 Ephb3 Eph receptor B3 2 10
MIRT583790 Elk4 ELK4, member of ETS oncogene family 2 2
MIRT584103 Csnk1e casein kinase 1, epsilon 2 6
MIRT587976 Armc1 armadillo repeat containing 1 2 2
MIRT590630 Arl5a ADP-ribosylation factor-like 5A 2 2
MIRT591969 Bbx bobby sox HMG box containing 2 2
MIRT591998 Wrn Werner syndrome RecQ like helicase 2 2
MIRT594081 Sema6d sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D 2 2
MIRT594218 Ube2k ubiquitin-conjugating enzyme E2K 2 2
MIRT594859 Nacc1 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing 2 2
MIRT595550 Celf2 CUGBP, Elav-like family member 2 2 2
MIRT595570 Mtf1 metal response element binding transcription factor 1 2 2
MIRT596982 Tango6 transport and golgi organization 6 1 1
MIRT599081 Eid2b EP300 interacting inhibitor of differentiation 2B 2 2
MIRT599176 Dhdh dihydrodiol dehydrogenase (dimeric) 2 2
MIRT600982 Denr density-regulated protein 2 2
MIRT602199 Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) 2 2
MIRT603280 Stambp STAM binding protein 2 2
MIRT603947 Grap2 GRB2-related adaptor protein 2 2 2
MIRT606257 Polr1e polymerase (RNA) I polypeptide E 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-452 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-452 Phenethyl isothiocyanate(PEITC) NULL 16741 Microarray neonatal mice liver 20145010 2010 up-regulated
miR-452 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-452 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 down-regulated

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