>Ubap2|NM_026872|3'UTR
   1 ACCCTGAAAAGGAGGGGGTGGGCTCTGGGCAGGAGGGAACACATGGAGCACCTTCCTGGAGCCCCGTGCCCTTGCTGGGA
  81 TCCTGAGACAACTGTCAGCTGAGCCCTGTGGGAAGCCTGCCTCCCAGACCCTCTGCTGTGTGTAATGTATTTATGTATGT
 161 ACTTGTAAATGTGATACAAGCCTGGAGGAGCTGGGCTGTGGTCACGGGTAGCACACAAGTGTGTGCTCCCAGTCCAGACA
 241 CCCTTACCATAGCCAAGTAGGCCCCACTCCGACCCCTCTGCCTTCAGATCTTCCATGTGGCCCCAGCTCAGTGGGCTGCT
 321 GGGTGTGCCACTGAAGGACTGGTGGTTTGGGGCCAGGTCAGTACCAATGAAGAATCACGACTTATCTCATTCCCTGTGAA
 401 CCATTATGTTTTCTCCTCTGTAATTACTTCATCCTTGTGTTTGAAAAACTGATCCTTCTGTCATTTGTCATGGTCCCTCT
 481 TCTAACAAATCTTAATCTGGAAACAGAAGAAATAACCCCTGTCCAGCAGAAGCCTGTCCGTCTGCGCACAGCAGAAGCCT
 561 GTCCGTCTGCGCACAGCAGGGCTTCCGCTTTGGGTCTGGTCCTGCTCTGGGGACTTGTCTTTCTAACCAACATTTAAAAA
 641 CAAAATCTGCACTAGGTCTCAGAGATGTGCAGCCAGTCAGCTCCCTTATAAAGAAATCAAAGATCTGCTTCCATCCATCT
 721 CAGTGAAAGCCTGTCTGCAATCAAAGGCTCCCCCATCCCAGTTGTCCTGAGCCAGGTGGACAGCTGTTTCAGGGTTTTCT
 801 CTCACCCAAACTCCACCTAATAAAATCCTGAGAGCAAAAAAAAAAAAAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' agauguaucuuaccguAGAGAGu 5' |||||| Target 5' agctgtttcagggtttTCTCTCa 3'	782 - 804	120.00	-12.90
2	miRNA 3' agAUG--UA--UCUUA---CCG--UAGAGagu 5' ||| || ||||| | | ||||| Target 5' agTACCAATGAAGAATCACGACTTATCTCatt 3'	360 - 391	115.00	-6.30
3	miRNA 3' agauguAUCUUACCGUAGAGAGu 5' |:|:: ::|||:|| Target 5' ctgctcTGGGGACTTGTCTTTCt 3'	602 - 624	113.00	-9.70

- Cell, 2014

MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.