miRTarBase - #MIRT580232


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miRNA Infomation
miRNA namemmu-miR-2183

Gene Information
Gene Symbol Tshz3   
Synonyms A630038G13Rik, Tsh3, Zfp537, mKIAA1474, teashirt3
Description teashirt zinc finger family member 3
Transcript NM_172298   
Expression
Putative miRNA Targets on Tshz3
3'UTR of Tshz3
(miRNA target sites are highlighted)		 
>Tshz3|NM_172298|3'UTR
   1 CGTTTGCTCCAGAGCGGAAATGACTGGAGGAGAAAATGCTGAAGGCACCTTAAAGCCCCTCTCTGTCTTGTTCTTGGCAC
  81 ACGTTCTTATGTTCACTGCAGAGGATTGTTCTGGGCTGGACTGTTCGGTCTAACTGTACAGTGTTTAATAGAGAGGTGCA
 161 TATAATCAGCTGTTGTTACTGGTAAAATAACGAAGGTTAAAACGCAGTGCTAAGTGTTTGGAACTTTGTGTAAATGGGAT
 241 TTAATTGTGAGCATCCCCTCGATGCTTCAAGCTGCATGCATTAACAGACGGTTTAATTAAGCATTTATAAGGAAACCAGG
 321 CACACTTTTTCCATGAGACTTGGGTGTGCTGGCATTTCCCACCCTTTCATCTTTAGCCCTCTGAGTACTACGAAGCACTT
 401 TTGCATTAATTTGGTTAATAAAAAAAAGATAATAATAAAACAAAATAATAATGAATAATAATAATGTATGAAACTCTGTT
 481 TTTAAAACTCGTTACCAGCTTAGTTATAAGGAATATTAGGAACCTCCATTTATGCAGGGGTATGACACGCCTTGAAATTT
 561 AAAAGAATATTATTTTCACATTGAAACATAGATGTATATATTGTATAGATTTCAAACTCTTATGGGAAAATGTGACTGGT
 641 TAAATGACCCTTTCTTTCTTGCATTTATAACAAGTGTTTTATGTATCTGCTATACTCTGGGCACAAGCCATGCCTCTGTA
 721 TAGTGTATATTTCCTTATCTTTTTCTTTTTTTTTAAGGTTTATGGTTTTTTTTTTACATGCAAACATTGTAAATTATACA
 801 GAAAATACCACAGATAGCATTTATAAAATATACAGAAGCATTATCTCAAAGCAAGGTATGATGTTTGTTTTGCTATACGG
 881 CACATCTATATTGATAGAGGGTTCATGTTTAAATTATACATATTTATTAGCATTCTATTATCATTTGTTTTGAGGTCAGG
 961 AAATCCATCCCTGGCAGGCATCATAACTATTTTGCACATGGTTCTTAAAGGTATTTATTAGAAATTAAAGGATGCTCAAA
1041 GTAAACTCAGTGCTCAAAGGGTTAAGTCTATTCGAAAAGGAAAAAAAAAGATTAAAAAAAAAAACCCAACTTGTACTGTA
1121 TTTCCTGAACATTGATAAAGCCTTTAAAATGTTTGTACTGTAATCCTTTGCTTAAAAGTCAAGAGTCGTTCTGTGGGACA
1201 GCCCCTTCACTTATTTATAAGCCCTCTCGGTTGCTAGTTCATAATGTGGGATTTTTTTCCCTTTCTCTTTTTTCTTTTCT
1281 TTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTAATTTCAATCGGTAACTTTGTTTTGTTCCTCCTAATTATTCTCCCAAGA
1361 TCCCACACTGCAGTTTTATCTTTAGGCTTATGAAAGGTAACCCGTGGTAACCGGCTCTCCAAGCCATTCTTGTCTCCATT
1441 TCTGGCAGTTAATTTGCAGAAATAACTGACAGCTGACACCATATGAGAACCTATGTATAAAATATTGGCATGTAAACACC
1521 ACAGACACCGTGACACACTCTGTGCCCTGTTTTGTTGTTGACGATGGCACACCATGATGTGACTCTTCATATAACCCTTT
1601 TTTTCTACGGCAGCATTAAAATTG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_172298 | 3UTR | AUGUUUAAAUUAUACAUAUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1385343
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_DM_Ago2_CLIP-Seq_myotubes
Location of target site NM_172298 | 3UTR | AUGUUUAAAUUAUACAUAUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
9 mmu-miR-2183 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT580232 Tshz3 teashirt zinc finger family member 3 2 2
MIRT582991 Iffo2 intermediate filament family orphan 2 2 2
MIRT583991 Dcx doublecortin 2 6
MIRT584568 Braf Braf transforming gene 2 2
MIRT584837 Arhgef3 Rho guanine nucleotide exchange factor (GEF) 3 2 2
MIRT585809 Smad9 SMAD family member 9 2 4
MIRT588831 Sp9 trans-acting transcription factor 9 2 2
MIRT595152 Ddx6 DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 2 2
MIRT598768 Gpr165 G protein-coupled receptor 165 2 2

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