miRTarBase - #MIRT581343


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pre-miRNA Information
pre-miRNA mmu-mir-873a   
Genomic Coordinates chr4: 36668510 - 36668586
Description Mus musculus miR-873 stem-loop
Comment This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-873a-5p
Sequence 11| GCAGGAACUUGUGAGUCUCCU |31
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Rgs17   
Synonyms 6430507P11Rik, Rgsz2
Description regulator of G-protein signaling 17
Transcript NM_001161822   
Other Transcripts NM_019958   
Expression
Putative miRNA Targets on Rgs17
3'UTR of Rgs17
(miRNA target sites are highlighted)		 
>Rgs17|NM_001161822|3'UTR
   1 TTTTCAGTTGACAGGCCAAAAAAAAAAAAAAATCCATTTCAGAGGGCTGGGATGGGAAATAAAAGTAATTAAATAACACC
  81 AGAAATTGAGTTCCTGAAAAACTACAGGTTGACTGCTGGGGAAATACAATGAGGAAGGTCTCTTGTCTCCATTTTTATCA
 161 AGGTTATCCATGATTCTGTTTGGGGCAACAACAACAAAAAAAGCCTATATGAGACAATGAATTGCCAAATTAAGTTTGTT
 241 TGATTCAACACTTGCTCTACTTGCAAGCAAATTGTGTTCCTAGTCCTCTGAACAATCCCCTAGTGCATCTCTAGAGGCCG
 321 CATGTGCAAAGTAAAACTGCTTCGTCTGCCCTTTACTTGTGCTATTAAATCAGTAGCATACCTTGTATCTGTATTTAAGG
 401 ACTTTTGTGCAATATGGTCTCTTAGAAACAATTGCCAACAAATTGGCCGTGGTTTGCATTTTTTAAAAGCATAATCCAAT
 481 ACACACAAAGGCTATTTTAAACATGTAACAGTGATATTTAACCATGCTACATACTGTGTTTAGTATACCGTCTCTGAAGC
 561 CAATTTTTCTGTACATGTTTAAAAAAAATAGAAGCTGTTAAAGGATCTTACTGTGTGAGCCCTCTGGTTGTTCACTGCCC
 641 AAATTCTGGCATTTTTGTTACAGAAGAGTTTACTATATAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA
 721 GAAAGAAAAGAAGAGAAAAAAAGGAAAGAAAGGAAAAGAAAGAAAGAAGGAAAGAAGAAAAAGAAAGAGAGAGAAGACAA
 801 AACTTTATGATAAGAAAAATTTTAATAAGCACAAAACAATCTGCAGATATCCTGTCTCATTGAAAATTCCAAGAGTGATA
 881 CTGTTTTTAGGTCTGAAAGAAGAAAAGAGTCTATGTATATATGAAATGTCCAGTACTATAATTGAGTTATTAAAGAATGG
 961 CTCCTCAGTTCTAAAAGAGTGATGCAAACCTCTGAATGTCAGCAGGTAAACAATAATAAGTTCGATAATCCAGTTTGGGA
1041 ATATAAAGGGAAAAGAACTAGTAAAGGAATGCCTACATGTAGCACTGTCAAAAGCATAGTGAGGAATTGCATCTGACTTG
1121 TGGCGTATTCACCTTGAACATATAATCCAGTGAAAAGCTTAAGTTTTTCCTAAGTAACACTTTATAAAGAATTACAAAGA
1201 AGAAAACCTTCAATGTCAGACCTTAACTGTTTTCACATAGTCTCTCAAGAAAGACTTATTTTAACATTGTTAAAAACAAA
1281 CTCTTAACCCTCATCCATAATTAAAAGGCAGTACAGTGCTCAAACCTTCTAGCTGCCAATATTATTTTTTTATAATTACA
1361 TTAACTCTTTACATGAAATACTCCCCTGACCACAGCATGTATTTATGACCTTCTGAACAGTATCGTCTTGTGCACAAAAC
1441 CAAAATTACAATCAATGTCATATTTATATCAAGGTCTTACCTTTCAATATCAAAATCCCCTGGAGCCTATTTTTTTAATC
1521 TTAAACTCAATGAGTATATGTTGAGTAACCTTTAAAAATGAAAGATTTAAAAAGATTTATATCCACTGAGAATACAGGCC
1601 TTATATTAAGGATGATATTAAAATTATAGGTTTTATTTGTCATTTTTAACTACCTCTCATATTTTTAATTTATTAAACAT
1681 ATTTTTTTAAAGCACAAGTTTGGAGTTTTCTTTTTAACTTTATTTTACTTGAAAGGTTGGCATCATGTTTCTTTTTCCAT
1761 AAATTTAAAACATTTCAAGAATTGTTTTTTAAGTGAATATGATTTTCTAATTTATATAGAATTCAGGAGCTATGATTCAA
1841 AGGGCTATTACATATTCCCTATGCAAATATTTTAACTGTTGCAGTGTTTGACAAAATGGGATTTTAAAAAAACAATTTGC
1921 AGAGTGGAAAAACAAAACGTTGTGTTTACACTGGCGCCGCTGACTATCCTACCATGCTCAGCACTTTTAAATACATTATT
2001 TATGACAATGTAGTTTAAATGCAAGATATTTATAATATTCATCTTTTAAAAAATGCCTGTGGATTTTTTTTTTTCCTGAA
2081 AGTGTTAGTGTGTTTAAGATACGCTTTGCTTATGTCACCTAGATGTTTACTATTTGAATAGTTTTGGTAATCTGTTGAGA
2161 AAAAAAGATAGTTATGAAGATGTTTACAAAAATATAATCCTATTTTTTCCTAAAGAGTCAAGAAAACGTAAAAGCATCAT
2241 GGCTTCTATTGTGAAGTCCCACCGTTCTGAATGATTTGATTCTTTCCTTGATTAGTGTTGATTATGCTAATAGGATGTAT
2321 ACACATGTATAGTGTACCATCATCCTCGTTCTGTGTTTGGGGCACAATGAGTTGGTGCACATGACCAGGAAAAATGTAAT
2401 ATATTACCACACCCTGCATGCATGATTGTTTTCGATGTAGACTTTATTATATCCCTGTTCCATGGATGCCAGTGACTGAC
2481 TGTCACAGGCGATCTTTACAATACGCTCTGGACTATTGGGCGAGCTGTCTGTGTGACAATGCATTTAATATTTTACCAAA
2561 TGGAAGAGATAAGGCTCCTTGAGAGAGTCCTCATCTCTAACCTAGGCATTTGCAGTGTTTGATTTCAAAGTTGCTGTGTC
2641 TTTGGAACCTGTGTACAAGGAGGTTCCAAAGCTGATTTGCACAGTCCCTGCCATTAGCCATTGCTATGTGAGCAGTTCTA
2721 TGCTAAATTCATATTTCAGGGGGATGGCTTCTTAGTTCACTTGAGAATGTTTCAAGTTTTGAAGAGTGTGTCTGTGGACT
2801 TGTGACTATGCCAGTCTTTAAAATATGGTGTGGTTTCTTCTGTTTAAGTCACAGAGCTATAATAAACAAACAAAAGCCCA
2881 CAACTGGTGTCTGCCACCATTTCAAGAGCACAACACTTGATATAGTCAAGTTTGATTTAATTCCTAGTGGGTGAATCGCT
2961 CCCTGGGGAGACCCTAGCACTTTAAATGAAGCACCTTATATTTACGATTGCCAAGATAACTTTGCATATTGTCAGAATCT
3041 GTTAATGCACACACAAGAGGATAACGCACTTTCTATAGTACCTGTCTATAGGCATATCTATTGTGGAAAGCAAAGGAGAT
3121 TGTGTAGGTGATGTATACAGCGGTGACTCAAAGGCAGATGTCTTCATAGGTGGATCTTACACATAGACATGCTAATGCTG
3201 AAGGGGTGACTCTTCTCTTTCTGACTTATTATGACATCATCCAAAGTTAGCACCGATGCTCTGATGGCATGAAAAGGGCA
3281 TATGAATGTTAACAAAACAGGATTTTGATACTGAAGTTATAAATGTAAAAGTAAAAATTACAATATGGCCTTTAAAACAG
3361 TCTTACAATATTTATATATGACCAGTTCTAACAGTGTGTCACTGCCTAGAACATTTATTAGTGCCCTTCAATGTCTCCTA
3441 GTATACAGAATGTGCTATATTTATTTGATAGTTAAGTGTTAAAGGACTATAGAAATAGATAAAGGCTTATCTGTTGAAAA
3521 CAAATGTACATCTCTTCCCATTATGCTAATTTAAAATACTTGAATCTTAATTTAAGTTCATTCTTTTATTCTAGAATTTT
3601 TTTAATAGTCTCAGAAAAATGTCATATTGTAATTGTGGAAACTACACTTGCAACAAATAAAAAACTTCAGGTGCTTTGGT
3681 GAAGGTTTTTAAGTGGAAGGAATGAAAATAAAGCATAGAAAAATGAACTTTTTGAAGAACCATCAATTTGTTTTTAAACA
3761 GGTAGAAGATACACCTAGAACCCGACATAACATGAATTTTTGAATACTTTCAGCTTTTATATATTGACATATTATTTTGA
3841 AACAGGAAATACGGTCAACAGATTGGGTTACCTATCTATTTAGATTGGCAGTTAATGACCAATCTCCAAAGTATCTATTT
3921 TTAAGTCTGTTTGAGTACTCTTTTCTGTTCTTAAATTTCTGATGATCATAAAATGCTGTCCCTAGAAAGGAGCATACCAT
4001 GTGTAATAATCTTAGCAATTCTCCACTTGCCACAATTGCAAATATTTACCACTAAAACCTACCAAACCTTCCTTCAGAAC
4081 ATGTCTGCAAGATTATAATATCGTCTTAACATCTTTGCAGTGCACACAGTGCATGGAGGAGCACTGGCTTTGATTTCTGT
4161 GGGTGTTAAAAAATATTTCTTTTACAAATAAAGTCCTAAGCAGCCATAAGGGACATGTTTTCTTTCCCACTGATTGAAGA
4241 GACTATACCAGACCATCATTCTGTAGAAAACAGGGAGATGGAGTGTAGCCTTCGTTGGGCAAATGGGGTTGTGGACTCTG
4321 ATTCTCATGACATAAAGCTCTAAGTTGAGAAAAAGCCATGACCTAAAGTATTCGTGATGATAGCCACATGTACTATTTTA
4401 ATGTCGTGCATGAAATCTGAAATTCAGTCCCCTCCTCATGTCTTATATCGTATGTCTTCTGTTGCAAAGTTTTCCATTGA
4481 TTCGTGCCTTTATCACAGCATTTTCAGCTTTTTAAACAGTTAGAAATATGTGAGCTTTAAACCGATGATTAAACAAACTG
4561 TCTAAGATTTGGGCACATTTCCTTTTGAAATGACAGCTGATTTCGAGTTGTATTTCTATCCAGAGGTGGTAGGGAAAGTC
4641 ACGTAACCTTTCTGAATCTGAATCTGTTTCTTTGTCTTAAAATATTGACTATGGGGTGTCCATGGGAACAGTAATGTTGC
4721 ATATAATAATTGACCGGAGTTCTTCAGCCTAGTAACAATAACATTTTTTGGAAGGTCTGTAGTTGGTGTCATTTCACTGT
4801 ATTCTCTCCTCGTGTTTCCTTGGAAAGTTTAGGTTAGCACACAAAGAGGGTTCATCATGACATTTTCATACCAGTGTCTC
4881 ATGATATCTTCTTCTCATTTGTTCCTCACTGCCTTTCCCAGGGCCTTCTACTTTCTTTGCTTATTTTTTTTTAAACATAC
4961 TTGAAACTTTAAAATGTGCATAGGTATGGACAAACACCATACACACACACACACACACACACACATGCATACAGAGACAC
5041 ATATACACACAGACACAGACATACAGTTATGCCGCCCACAGACACATGCACAAGCACACATACACACAGACACATCGAAA
5121 CATCCACACACACCACACACACACATACAGAAATACACACTCAAAGATGGAGACATAGGCAATAAAATGACCATCCCTGT
5201 GGGTGGGAATACTATAGAATCAAGTTAGTTACCAGTATGCCAAGTATGCCAGTGGGCAACAGAGCTGAAAGCAATGTCAT
5281 AAATCATGATTCGAAAATAATCCCTTCAATATTAGTGTGACTTTGTCGATTCTATCATGAAACTATGAATAGAAAGATGA
5361 ATACAAGTGGCTAAGTGTTCATTAACAGTTTCACCAAACTTTTGAATATTTTCAGCTTTATAGCAAAATGATGAGGCAGT
5441 AAATGCCCTCATGTGTTCTATGTGTCTGGGAAGCAATTTTCCCAAATAATAACTAAAATTGACCCATCGAGTCTGTGTTC
5521 CACTCCATCATCATCCTTCACCTCTTGCCTTCTCTGCCCTTCCCATCCTGTAACCACACGACCTCCTGTTGTTTCCTCTC
5601 TTTGTGTTTCTTTCAGCTGTGACTCTCTCTAAATGGTCTTGTTTTTTCAAAACCTTGACAGTTGTGAGTGGAAGTGATGT
5681 CTATTTTACAGAGCATCGCTCTGCAGGGTGGGGTTTGTCAAGACTTCTCCTGTTTATATGAGTGGGGATTTTTTTTTTGT
5761 TTTTGTTTGTTTGTTTTTAGTTTCTGAAAGGAATAATGTTATTCTGTTCTTTCTATCATAAATATTTGTTTTTATTTTAT
5841 GTGATTGAGTGCTTTGTCTGCATGTATGTATGTGACCACATGTGTGCAGCACTTGAGGCCAGAAGAGTCCTCTGGAATTG
5921 GAGCAACAGATGGTCTTAAGCCTCCATATGGGTTCTGGGTACTGAATCAGGGTCTTCGGTTCTCTTAACTGTTGAGCCAC
6001 CTGTCCAGCCCCGAATAGCCAACCTTATTACATGATATCTACTTGCCACTAGGGTAAGTACTCTTGCTTTGGGCTCCTCC
6081 TTCCTATTGCTGTTGCTGTTCGCCCTGTCACCTGATTTAAGCATTGCCTGTTGGGTACAGCCTCTGCTGCCAAGTGATCC
6161 ACATGCAAAGGTGTCTTAGAGGGAACTAGACCCTGCATTTACATTGAGCAAGAAAAACAACCAACCCCACCCCCCATAAA
6241 AACAACTCTGGCCAATTTCTTAGCATGAATACTAGGCTCCATTAAAGGATTACTAATAAATACTATAAAGAAAATGCAAT
6321 GGATTAAGAAGTGAAATAAGTCCGAAGCAGTGTTAGCTTGGCTATCTACCTAATAAAAGTGCAACTGTTCTCTCTTCCCT
6401 GAAGCACCCTGCATACTTATCTAGTTCCTGCACACATGCTGGATTTGAAGATTTTAGCAAGAGGTTATTTATTACTCGTG
6481 TTTAATGCCTTCCTATATACCATCCCCTGAGCAATGTATACAATGCTGAAGTATAGAAAATTTGATGTTGTTCCTCAAGC
6561 TTTAACCCGTTTGTCATAGTGTTTCTTGTGTGTGTTTTTAAAGAGTTTGCAAAATACTAAAAAAAAGTATTTTAATGAAT
6641 TTGATTCTGTGAAATACATCATTTTGAACATGTATAATTTCTTACTTTCATGCTCATTCTATTTAGTGATCTATTTCCAT
6721 ATTTTGTATTCTATCAATATATTTATTAGTGGACCACAAAAAGGTTCCGAGTACATGAATATAAGAGTAGCCCTGCTTGG
6801 CTATTTTATAATACCAAAATACCTGTGGGTACTCATAGTTGAATAGATACTCCAGATTCTTTTCCTTGCAGCTCAAGTTT
6881 GTCTGGGTTTTTCTTCCATTGAACAGATTTCATCATTTCCCTATGTACAATAGTAGACTTCCTGAAAGTGCTACAGTTTT
6961 TACCATGTAATAGACTTGAGCATGACAATACTGTAAGAAAATACAGAAGTCTCTAAATATGTCTGAGTTTTCAAAGGAAA
7041 TCAAATAATAGGTGTTTTCATTTGACTATATTGTGGCATTTATGGGTATTACTGTAAAATGCATATGCATTACCTGATTT
7121 TCTTAACATGTTTTGAACTAATAAAGAATTCTCCCATGTATCCTTAGACAAGTTATTGTTTGCTTATTGGTGTAATGCTT
7201 ATCTGAGTATAACAACTTCTGGGTGGAGTCTTCACATTGTTATGTAACATTAGAGTACCTTTTTATAAGAAATAAAATAT
7281 GTTCTTGAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uccUCUGAGU-GUUCAAGGACg 5'
             | |||:| | |||||||| 
Target 5' tgcATACTTATCTAGTTCCTGc 3'
 6410 - 6431 162.00 -17.80
2 
miRNA  3' ucCUCUGAGUGUUCAAGGACg 5'
            || |:|   :|||||||| 
Target 5' caGAAATT---GAGTTCCTGa 3'
 80 - 97 152.00 -13.20
3 
miRNA  3' uccucuGAGUGUUCAAGGAcg 5'
                ||||:  ||||||  
Target 5' cttcttCTCATTTGTTCCTca 3'
 4888 - 4908 135.00 -14.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
17 mmu-miR-873a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT438380 Tnfaip3 tumor necrosis factor, alpha-induced protein 3 3 1
MIRT577099 Wdr26 WD repeat domain 26 1 4
MIRT577463 Trmt61a tRNA methyltransferase 61A 1 3
MIRT577540 Thnsl1 threonine synthase-like 1 (bacterial) 1 1
MIRT578417 Kcnj11 potassium inwardly rectifying channel, subfamily J, member 11 1 1
MIRT578529 Ifit1 interferon-induced protein with tetratricopeptide repeats 1 1 1
MIRT581343 Rgs17 regulator of G-protein signaling 17 1 1
MIRT584008 Dcbld2 discoidin, CUB and LCCL domain containing 2 1 1
MIRT586514 Ndufaf4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 1 1
MIRT586607 Mob3c MOB kinase activator 3C 1 1
MIRT589481 N4bp2l2 NEDD4 binding protein 2-like 2 1 2
MIRT591646 Tmx4 thioredoxin-related transmembrane protein 4 1 2
MIRT593307 Stx16 syntaxin 16 1 1
MIRT598176 Mrpl45 mitochondrial ribosomal protein L45 1 1
MIRT602468 Dnajc18 DnaJ heat shock protein family (Hsp40) member C18 1 1
MIRT603743 Mrpl41 mitochondrial ribosomal protein L41 1 1
MIRT604791 Pik3r1 phosphoinositide-3-kinase regulatory subunit 1 1 1

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