miRTarBase - #MIRT581518


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pre-miRNA Information
pre-miRNA mmu-mir-325   
Genomic Coordinates chrX: 105379082 - 105379179
Synonyms Mirn325, mmu-mir-325, Mir325
Description Mus musculus miR-325 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-325-3p
Sequence 54| UUUAUUGAGCACCUCCUAUCAA |75
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Ptprd   
Synonyms 1110002J03Rik, 3000002J10Rik, B230219D21Rik, R-PTP-delta
Description protein tyrosine phosphatase, receptor type, D
Transcript NM_011211   
Expression
Putative miRNA Targets on Ptprd
3'UTR of Ptprd
(miRNA target sites are highlighted)		 
>Ptprd|NM_011211|3'UTR
   1 AAACCCCTGACCCCTTCTGGATTTTTACTACAGGCCCTTCGCTATCCATGGAGTCTCTTCTGAGCCATACAGGGCACTTG
  81 AGAAGTCCTTCTTAACTTCTAGCTAACAACCACTTAGTGGGACTATTACAAAAAACAAACAAAAAAAAAAAACAACAAAA
 161 AAAAAAAAAACAAAACCAAACTCTTCCAAGTGGACCAAGAATTCACAGTTTAATCATCTAACCTGAAAAATAATAACAAG
 241 AATCCTGACTTAGGAGGCATCTGAAGAATTCTATTTACAGATACCTCAAGGATTCAAAATAAAGGGAAGAAGAAATGACA
 321 GCAAAGAACCACCATCTTGTTCAGGATTGCTTGATAGGGACAGAGAGAGCTTGCCTGTGGGCCTCAGGCCAGCACAAGGA
 401 ATTCGCTGCTGTGGCTGCTCACATAAGGTGGACTCTGCCTCAACATCACCCTTTCCCCGTGCCACTCATACTTACATTTT
 481 AGGGGGAAGGGTGGGGATGTTTAAAAAAAGAAAGTCCTTGATTGCATTTTTATGTTGTAAAGATTCTGCTGACCTGTGCT
 561 TCATTTCTAACTATGTAAACTTTTTTTTTTTAAATGTATCATTCGATAAAGTAAACTTATAACGTTAAGTAATTCTTCAT
 641 ATTTTTTTTATTTCTAAATTCTAGGTCTTTTTAAGCTATAGAATGGTTATCTGTAATATCTTACTGTAGAAATATTGAAT
 721 AATCTGAAACTTTGCACATTTTTGTGCAATGCTCAGTCTACAGTACCTGTCTCAACAGCTATTACTCACATGTTTTGTTT
 801 TGTATAACCGATGAGAAAGTGCCCATTCTCTGCAAGCTTCAAGGGGAATTTTGTTTTGCTTTGTTTTCTTTTTGGAATCA
 881 ACAGGGAGGCGCAAAGTAAAAAGCTGCTGCTAACATGTATATATACATAGATATCTATATTTTAAGGGTATCTATGTATA
 961 TATAGACAGTATGTCCACACAAAGATTTAACTGCCATTCGGTTCTTCCATGGTTTAGGTTTTGGGGGGTTTTTTGTTTGT
1041 TTGTTTTAATTTTTTTATTTTTAAGTATAGAAAGCGATTGTAAACATCTTTTTCAAGTTACTTTTAGTATTGTGAAACTT
1121 TAATTTGTAAAGGGAATTGAGGAGTGCACATCATTGATTATTGAGCCTCGCCCCTAACTTTCCAGCCTCGTATTCCCCCA
1201 TCTATCCAACCATATCCACAAATACTGTTTTGTTAGCCAGGCTTCCGACAAAATTCAATATTTCTAACACTCTAGTGCAA
1281 TAACAATTGTTAAATATCTAAAAGGTGTGCATGTGGGAAAGGTCAGTGCATATCCCTTTAGGAGGGAAGAATGTTGTAAT
1361 ATATCAGCTATCAAGTTGTTGGTTTTTTTTTTTTAAAGTATATTCAATCATATATTGTCTATAGTACGTGCTATGAAATT
1441 TGCATTTATGATATGTAACAGGGGCAAAGCCAAACTCATGTTACTCTTTTCAGTCAGAAACATGTTGTGTCAGCCGCGTT
1521 CCTGGGAGGCGCTGTGCTGTTTCATTCGGTTTTAGTTCTGCATTTAGAGTGCCTTATAATTGATACCTATTTTAATAACA
1601 TTTCTTTTTATCTTTCGGTTCTGATTTCCATTAGATGTTCACATTTGTTTCTCTTTCAATTAAAACATTGTCTGTCCACC
1681 ACATGTATATAAACTCTTTGAATAATATGCATTCCTAGTTTTCAGCCAAGTGGGGGATGTTAGTGACTGTACCAGCCCAA
1761 AGCACTTGGATAATCAGGGCCTCTCCTTTCCTCATTGTCACATCAGGAAGAGCAACTTGGTTTACTTCAGATCCCTTCCA
1841 AATCTGCTCCAGAGTGGGCAGTAACCACCAGTCTTACTTATAGAAACAACTTCGGGATATATTTGATATTCCATTAGGAT
1921 TTTTCTGAAAGGGGCAATAAATTACATATATATATATATATATATATATATATATATATATATATATATATATATATATA
2001 TATATATCTCCCCAAACATTTCCACAGGAAGCCATGGATGATGGCATGAATTTGCCACATGCTATATGATTTTAAAATAC
2081 CCTCCACAAATACTGAGTACTCTGAAAGAGTTTTGATACAGGTGTAATATTTTGGTTTAATTTTAGCTTCATGATGGTCT
2161 GCATGGAAGGGTATTTGGTTTCACATCTTCCCATCACTAGCAGAATAAAATCCTAGAGACCAAACACTTGCAACTATCAT
2241 ATCTGAGCATTTTTTTTTGTAAAAAAAAAAAAAAAAAAAACAAAAAACAAAAAATAAAAATTAAAAAAAAATGAACAAAA
2321 ACAAAAAAATAGAAAAAATAGAAGTATATATAATACTAAAAAGAAACTAGAAGGCTACCTCAGAATGAGACTCTCTAACC
2401 TACATCAGAACCAGATAGAATGTGCACTGTGTGAGTCTATTGTTTTCTTTTCACTTGTACCATTGAAGGTTTATCCAAGT
2481 GCCCGGTTGCTCAGTGCTGTGAATTTTGTTTTAAGTGAAACAAGGAAGGTCAGATAGGTTATTGTGTCACCTAGACATGC
2561 CATTTTGGACATGTTGGATCTAATGGAATATCACACACCAGAATGACTGGCCAGTGAGCTGTTTGTCTCTTGGCAACAAA
2641 ACTGCCTGTATGGCAGGAACAATGACAGTAATCAGCAAAAGAAATGGATGGGACGTGTCCTGCAGGAGGATGAGGTGGGC
2721 GTTACTCCTGAATCAGGAGCTGGTCCTAGAACTAACTCCCCACTGTCACTGATGTGCATTCCACTCTGCTTTGCTTTATG
2801 ACCATTCAAGTTTAATTTCCCAGGCAAACATCAGTATCTACCATTACCATAGACATCAAAGTTTCCAGTTATTTTCATCA
2881 TCATAACCAGTACGGTGCTATTATTTACCTATGTATGTGTAGTTATGTATAATTTTGTAATTAGTTACAATGGTAAAATA
2961 TCAAATATATAAAAGTGATTTGTACAGAACTTTATTTTAGCTCTTTTTTAAAAATGGTTTGCATGGTTAGACAAGGCAAG
3041 GACAGCCAGGGGAGGGAAGGGCCTCTAGGGAACTTCGCACTTTCTACACCTTTGTACTATGCACTGCTCTATTGATTCTA
3121 CACCCAATAATGTTATTACTTGAACCCATCTGTAAGGAACTGCTTCAGAAGTTCATTTCTGTGTCTGTGGATAACAACAC
3201 ATCACAGCAGGAAAGGAAAACATGCCTAGTAGCACACACAGTTTTTTTTTTTCTTTCCTGTTGAGTTTTGGGTTAGCTTT
3281 GTTTCAGTCTTTCTTGTCATTTTAAAATTCCCTTCCCATTTTTTTCTCCTTTGCTTTTGATTTCCATTGGGTATATGGGT
3361 GCCCTGATACTCCAGCAGAGGTCAGAAGGCTACAGATCCATCCTATCCATCCGTTACATGGCTTTGCCATACAAGCTTGG
3441 AGTGTCTTTACAAAGATAACAGTTGTGTTCTTTGCTCTCGTTTTGGATGCATAGACTGAAAATTCAAAAAAAAAATAACT
3521 TGTAAAATGGCTTGTTAAAAAAAATACAATTACCTCTAATTAGTAGTACGCGTAAGTGTTTTACAGAATGAAAGGCGTGC
3601 TTTTTATTTTCTTACTTCGTTACATTGGTGGCGAAAGAAGTCTGTATGAAAATCAGTTCTTTGCTGACACAGGTTCCATT
3681 TGTTACAAATGAATTCTAATAAAAATGTCAGTGTTATGCAG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aacuauCCUCCACGAGU-UAUUu 5'
                | :| |||||| |||| 
Target 5' gctgctGTGGCTGCTCACATAAg 3'
 405 - 427 128.00 -10.20
2 
miRNA  3' aaCUAUCCUCCACGAGUUAUUu 5'
            || |||:|     |||||| 
Target 5' ctGAAAGGGG-----CAATAAa 3'
 1925 - 1941 126.00 -9.90
3 
miRNA  3' aacuauccuccacGAGUUAUUu 5'
                       :||:|||| 
Target 5' tttaaatgtatcaTTCGATAAa 3'
 589 - 610 125.00 -6.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
27 mmu-miR-325-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT054600 Arc activity regulated cytoskeletal-associated protein 3 1
MIRT577829 Rinl Ras and Rab interactor-like 1 1
MIRT577905 Prr11 proline rich 11 1 1
MIRT578380 Krtap6-1 keratin associated protein 6-1 1 1
MIRT578550 Hsd17b1 hydroxysteroid (17-beta) dehydrogenase 1 1 1
MIRT578770 Gemin8 gem nuclear organelle associated protein 8 1 1
MIRT580288 Trhr thyrotropin releasing hormone receptor 1 1
MIRT581518 Ptprd protein tyrosine phosphatase, receptor type, D 1 1
MIRT582779 Kif1c kinesin family member 1C 1 1
MIRT585752 Stard6 StAR-related lipid transfer (START) domain containing 6 1 1
MIRT587310 Ephx3 epoxide hydrolase 3 1 4
MIRT588735 Taok3 TAO kinase 3 1 1
MIRT592550 Mup7 major urinary protein 7 1 1
MIRT592592 Mup13 major urinary protein 13 1 1
MIRT594207 Wdr12 WD repeat domain 12 1 1
MIRT594462 Epyc epiphycan 1 1
MIRT594693 Atp11b ATPase, class VI, type 11B 1 1
MIRT594929 Gjb2 gap junction protein, beta 2 1 1
MIRT594934 Frem3 Fras1 related extracellular matrix protein 3 1 1
MIRT595784 Hlf hepatic leukemia factor 1 1
MIRT595846 Set SET nuclear oncogene 1 1
MIRT595897 Cd69 CD69 antigen 1 1
MIRT603010 Klk8 kallikrein related-peptidase 8 1 1
MIRT603579 Ppm1k protein phosphatase 1K (PP2C domain containing) 1 1
MIRT604052 Esf1 ESF1 nucleolar pre-rRNA processing protein homolog 1 1
MIRT736870 GHRHR growth hormone releasing hormone receptor 2 0
MIRT756335 Gsdmd gasdermin D 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells CLY cells 20955366 2011 down-regulated
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells HT-29 cells 20955366 2011 down-regulated
miR-325 Cisplatin approved 84093 Microarray CNE cells 22614822 2012 up-regulated
miR-325 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 up-regulated
miR-325-3p Urocortin 2 NULL 56843276 Quantitative real-time PCR anterior pituitary cells 22252941 2012 up-regulated

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