miRTarBase - #MIRT581568


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pre-miRNA Information
pre-miRNA mmu-mir-5114   
Genomic Coordinates chr19: 44303171 - 44303231
Description Mus musculus miR-5114 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-5114
Sequence 1| ACUGGAGACGGAAGCUGCAAGA |22
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Prtg   
Synonyms A230098A12Rik, Igdcc5
Description protogenin
Transcript NM_175485   
Expression
Putative miRNA Targets on Prtg
3'UTR of Prtg
(miRNA target sites are highlighted)		 
>Prtg|NM_175485|3'UTR
   1 ACAGCCACGCTCAGGGATTCACAACAAGCACCTGTTCGAAGGACAATGAGCAGGGAGCCAAAGCCTTTTGTGAAAATCTT
  81 GATGTCTTACTGTTTATTGTTTGTTTTGTTTGTTTGTTTTTTATCTTTTTTTTTAAACTATTTTTAAGCTTACGAATTTT
 161 GAATGTTTCAACTGTCAACAATGTGAAAAAGGGCAGTCAAGATGCTTGACTGGACTCTAAGATAAATCACTGGAGCGGAG
 241 GGAAGACTGCCTAAGCCCTCTGCTGAATAGCTACCTCAGTTTGCTGTGGGCAGACTCTGAAGACAACTGTGCTGCCTTCT
 321 TCGAGTGCTGGTTTCTCTCAGTGGCATAACCAGCGGGACTTGCTAGTGACGCTGTGTGTCCATTGTGTGTGTGCTGTGTG
 401 TGACTGAAGGAGTCCGATAGGTAGGAGAGGAAAGATGTTGATCTGAAGCTCTGATTTTTTTTTTTTTTTTTTAAAGAACC
 481 GTGATAATGTAACACCTCTGGCTGTGAGGAGAAAATATGGGAGTATATTTATTTGAATGGAAGAAACAAATACGTCTCTT
 561 TGGAGAAGAGAACATTCCATAGTACATAGGGGATCGTGTCCTTGCAAAGCTGTTGGGTTTGGGACACCAGAGCAGGAGAA
 641 TTTTGCTCCAGCCTGCATCAGCCTGCCCTCAGCCTGCAAAACCACCTTTCCCTTTGCTCCACAGCCGGCCAAGGGCTGCG
 721 CTGTCTTGTGGAACGTTTTCAAAGTTTACAGAGCTAGCTGCAAAATACAATGTAGGAAAGTGGATTTTTTTTTTAATGGT
 801 TCAGATTTTCTTTTTTAAAATCTCAGTTTGGCTTCAAATGGGGCAAAGAAGAAATGTTATTAAATATTAAGAATGAAGAT
 881 AAACGAAACAGTAGAGATTAAGACAGACTTGAGGTTTTCATAATAGCAGTGCCTTCCATTGTGAGAAATTTTGCCTTATA
 961 CCTTTTTAATATTCACGAAGATGTTTAGGAAAAAAGGTACCAAAAATTTGCCTTAAAAAAAAAAAAGCTTGGATCTTAGC
1041 GATAGCTGTGCTATCTGGCACCTTGATGAAGCTGCTCTCTTTTTGAAGCTCAGGTTTGCTCCCTATCACCCTGGCGGCTG
1121 AACTCTGGCTTCGTAGCCCACTAACACTCCAGAAGCGAGTTCATTCTCTGGTTTAAAATCAACAAATTGTCTCCTCTTCT
1201 GATCCGGCAGCCTTCTCCGTCACAAGGCAACATGCTAGGTGCCGAGCCACGTGGGAAGGACAAAGACTGAGCTGTGAAAA
1281 GAAGAAACAAGTCCTTAGCCTTTTAAAATCATGAGTTTTGCCCTCCCTGAAATTTTTTTGGAAGTGGCTTTGAATTGCAT
1361 TTTAAAAGAAAAGCCCTCCGTGGGTTTTCCCTTGAAACCGTCCCTCCATGCACAGACAAGGCAGGTCCTGCCAAATGGTG
1441 TTATCACCCCAGTGTCATCTATTGCGAAAGCCTGACACTGCCACAGTTTTCATCGTCATTCTAAGTTTTGTGCCAAATTG
1521 GACTTTCTTAGAAGCCCAGGCACTACTCAGCACAGTTCTGTCCCATTCACCATGGGCTTCTCGGCAAAATGGGGGGAAAA
1601 AAGAAAGAAGAAAGAAAAAAAAAAAAAGCAGAGCTTTAGGTAAACAGTCGGAGGGGAGAAAAAATGGCTTCCTTCCCCTG
1681 CCTTCGCGCTCCTGTTTATACTACCTCCTTTTTAAAGTTATTCTAGTGTTATTGTTCTTTCCTGTACTCAGGGACTTTTT
1761 TGTTACTCTTGAGCTCATGTTGCATGGGCGGGTGCATTGACAGGGTATGGTGGTGTTTGGTTCGTAAGTAGAAGTACGTG
1841 GGCCAACTTCTCTCAGTATCTAAAATGAAGGGCACAATGGGATCACAAGTCAGAAGCAAACAGATCGAAAAGGGGAAAGC
1921 AGGTTTAAGGCCATGCATATTTAAACCGTGTTCCCTGCCTGGATCATCCGAAAGGCTGGGACAGGACATTTCTGGAAGAA
2001 CGTGCTCTGTTTAATGATAAATAGGCCCTTAGTTATGATAGGTCTTTAGCTTATAATTATTTACTAGTTCATTTTTTGTT
2081 CTTATACAGACTTTTCTGTCAATTCTGTTACCAAAGAATTTTCAGTAGACAGTAAAATTTCTATGATAAGTACCTTAAAT
2161 CCAATTCACTAGAAACAGATATAGACTCGAATATAAACAATTTCTAACCCTAATCTGAAGGGTTTATATTGATAGTTAAA
2241 ATTAATTAGGCTAGTGACATAAACTACCTTACCGTCACCAGAGTTTAAAATAAATTTTAATTTCCTTGCCAGCATATGGT
2321 TACAAACTCTTTAAACTGTAAGGTTTTTTTTTTCTCCAGCAAGTAACGAAACCTTCAACTCTATAGTATTAATGTATTTT
2401 CTAATTGTTGAAATTACTTGTTGGAGAGAAAAAAATAAATATTACTACTAATGTCATGGACTAAAGTATTTAATGTTCAA
2481 CAGAGGTTTTAGGCTTGGTGAAAGCCAGCCTCTCTGAAGTCTGAAGTACGAAATTCCAACTAAAGTGTGAAGGTTATCAT
2561 GTCTTCCTCGAGGTTTCATTATTCATCGTATTCAACTCCAAATAAGCTGACATGCAAATTAGAAACTGTTTCATTTTCCA
2641 AAAACGTATTTGCTGGAAGCGGTGATAACCTGGCTGCCTGTCATTTTTTAATGTCAAATATTACAATATACTGCTCATCG
2721 GTATTTTCATACCAAAGACCATTAAAATATATAGTTTAGCCTGTAAGTAAAGATGAGCAGGTTTAAAGTAAATTTATTTA
2801 GAAACAGATAGGGAATATAGCTTTAGTGGCAAAAAGAAAATATCAAAAGGCTAAGTTTACATAGTTAAAAGCAATACAGT
2881 TATATTGGTATTTATCAGTGTTTTTTATTCGAGTACTTTATATCAGCAAATTAAAAAGAAATTAAAAACCAAAGATATTT
2961 AAGGTTACCTCAGAATATAGGAAAGTTTAGCTGTACATTGAACTGTTGTTTGTTGTTTTGTGTTAACAAACTGTCTCTCC
3041 AGAACCTCTCCTCTGTGCAGACAGCGTCCTGAGGATTTATTTCCATGGTGTCTGCTCACTCAGTCTCAGGTGTGTTTTTA
3121 CTACATGGTGTGCTGCTTCCATACAGCCTGTGAGGGTGATAGGTCGGCAGAGAGAGCATCAGGCTTGTCTGTATTGTGGG
3201 AACCCCTACATCATTCTAAGTCGTCTGCATTCCTATGTAAAGTCCTTCTCTAGATACTCAGACAGCTTGAGGAAACCAAA
3281 AGGTGCCACTGTTCGGACGGGCAGTGCTTTGGGTTCAAATACGGTTCCTAAATCATAAGATTTTCATTGCGTCGAAAAGC
3361 TGGCTTTCGATCTCAAGTGAAACCCTGTTAAAGTGCCAGGGGGTGAGATGCTCCTGATGTGTTTGTTAGAAGCTCTCTTG
3441 CCTTTACCTCAGACACGAGCAATGGCAGCAATGCAGCAGAGACTGGGGTACGCACTGCTGTCTCTCTTCTGCAGGACTTC
3521 TGCAAACAGCATAAAAAAGGCTCATAGCTGACCAGTCAGGAGCACCGCTTAAGTCCCCGCCATTGGAAACAGTGCTTCCT
3601 GTCATCGAAGGCTGGGGTGGTTCACTCCTGTCATCAATTGCAGCAGTCGGAGGAAGGGGGCAGCATGCATTTTAGGAAGT
3681 CAGAGCCTGCTGTAATTCATGGTTCGATTCTGTACATTTGCATATCATCAGATGTCCATTTTGAATACCAGATGTGGAAA
3761 AAGAGTTTGAACTGTATTGTAAAGAGCTGGTACCTGAAGCAACCATGTATTTTGCAAGATTTTCTTCCTGAAAATTTACT
3841 ACATGCATCACTGATAAAATTGCTTTGGTAGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCACTCCTGGGCTG
3921 TGTGAGCACAGAGTGGTGGGTTCTGGAGACCACCACCCATATCTGTCTGTCTAATTGTGAATTTTGTTGACATTTAGAAG
4001 GCAACCATAGGTTTTATGCCAGGTTGTCCATGCTCAGGACACAAGTTCACAGGCTATCGCCATGCTTCTGTTACAGGCTT
4081 ATCTAGCAGACGGGTACTGAATTCATTTTCTTGCAATTTTTCTAACAAACCAGCACGAACTTAGTGTCTGTAAACAGCAC
4161 CGCACAGTGTTGAAGGTCTGTAGGTCAGAACTCTTTCTTTTTGAGGCAGTTTCCTGCTGTAAAACCCTCTCTGTCCTGGA
4241 ACCTGCTGTATGTATAAACCAGGCTGGCTTTGAACCAGTGGGGACCCTGCTGCCTCAGCCTATCTTTAAAAAACAAAACT
4321 GCTTTTCAATGGGTTTTTCTTATTTGCCCTGTCCTGTAGTATCTGGTCCATTCCCAAACTAAAACCCTTCCTGCAGATAA
4401 GCCTGAACACAGATGTGCCTCTGCCTCTTAGTAGGGCTTAGGAGATATATTAAGAAAATGTGTCTGTCAGTTACTGTTGT
4481 TGGACAAAAGTTTATGTCTGTATGTGTAGGAATTTTTTTTCTATTTCAAAGATGTGAATTTAATGAAGTGTGGGAGCCAG
4561 TTAAGTTATTTTCTGTAGTGGGTGTTCTGGGTCCTGCCACTCTCTGCTTCCTTGTAAGGGCACCATTTCCTGGCTTCCTG
4641 GTGGACTGTGGAGCTGGGTTACATTCGATTTCTGCTTGTCTCAGGGGACCAGCTCTGCAGGCTCCATCTCATGAAGAAAG
4721 TGAATGTTTGCCTTGATTTTGAATGTATTTTTAAAAATGTCTTCTTAAAACATATTTTTTGTTTAATAATGTGGGTTTGT
4801 TTTCTTTTCCTAGCATGATAGTAAATATTGCTTAGTACCCCACATCCCTACCCTACCCCTGGAAGTTTATATTCTACCCT
4881 ACAGGTCATGCCAAAAATGAATAAAAGTTTAAATGCCAAAATGTTTATTGAAACTGCTGTCTACATATTGATTGATTGCA
4961 CAGTTTAAATAAAACCTTTCCTTAGTTTTAAAAAAACAAAAACAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agaacgucGAAGGCAGAGGUCa 5'
                  :||:: ||||||| 
Target 5' taaggtttTTTTTTTCTCCAGc 3'
 2339 - 2360 150.00 -12.70
2 
miRNA  3' agaacgUCGAAGGCAGAGGUCa 5'
                | || :| ||||||| 
Target 5' ttaacaAACTGTC-TCTCCAGa 3'
 3023 - 3043 147.00 -13.00
3 
miRNA  3' agaACGUCGAAGGCAGAGGUCa 5'
             ||:|   ||| ||||:|| 
Target 5' ctaTGTAAAGTCCTTCTCTAGa 3'
 3233 - 3254 143.00 -14.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' agaacgucGAAGGCAGAGGUca 5'
                  :||:: ||||||  
Target 5' ---gguuuUUUUUUUCUCCA-- 3'
 		1 - 17
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM1385343
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_DM_Ago2_CLIP-Seq_myotubes
Location of target site NM_175485 | 3UTR | GGUUUUUUUUUUCUCCA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
39 mmu-miR-5114 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577166 Madd MAP-kinase activating death domain 2 12
MIRT580377 Tmem206 transmembrane protein 206 2 4
MIRT580729 Srrm4 serine/arginine repetitive matrix 4 2 2
MIRT581165 Sec23ip Sec23 interacting protein 2 2
MIRT581310 Rnf150 ring finger protein 150 2 4
MIRT581568 Prtg protogenin 2 2
MIRT582280 Nav2 neuron navigator 2 2 2
MIRT582511 March5 membrane-associated ring finger (C3HC4) 5 2 4
MIRT584183 Cpeb3 cytoplasmic polyadenylation element binding protein 3 2 2
MIRT584252 Cmtm6 CKLF-like MARVEL transmembrane domain containing 6 2 2
MIRT584403 Cd63 CD63 antigen 2 2
MIRT586848 Ikbkg inhibitor of kappaB kinase gamma 2 4
MIRT586939 H2-T24 histocompatibility 2, T region locus 24 2 4
MIRT587266 Fam161b family with sequence similarity 161, member B 2 2
MIRT588386 Zfp113 zinc finger protein 113 2 2
MIRT590219 Dync1li2 dynein, cytoplasmic 1 light intermediate chain 2 2 4
MIRT592159 Paxip1 PAX interacting (with transcription-activation domain) protein 1 2 2
MIRT592188 Nanog Nanog homeobox 2 4
MIRT593309 Rmnd5a required for meiotic nuclear division 5 homolog A 2 2
MIRT594051 1200014J11Rik nuclear cap binding subunit 3 2 2
MIRT594495 Cspg4 chondroitin sulfate proteoglycan 4 2 2
MIRT595572 Mtf1 metal response element binding transcription factor 1 2 2
MIRT597122 Svopl SV2 related protein homolog (rat)-like 2 2
MIRT598947 Ggps1 geranylgeranyl diphosphate synthase 1 2 2
MIRT599498 Ccdc78 coiled-coil domain containing 78 2 2
MIRT601067 Cdc14b CDC14 cell division cycle 14B 2 2
MIRT601902 Padi2 peptidyl arginine deiminase, type II 2 2
MIRT602607 Chrna1 cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) 2 2
MIRT602771 Armc2 armadillo repeat containing 2 2 2
MIRT602877 4931428F04Rik RIKEN cDNA 4931428F04 gene 2 2
MIRT602958 Xpo7 exportin 7 2 2
MIRT603304 Slc4a7 solute carrier family 4, sodium bicarbonate cotransporter, member 7 2 2
MIRT603315 Slc35d2 solute carrier family 35, member D2 2 2
MIRT603389 Sgol2 shugoshin 2A 2 2
MIRT605052 Efr3b EFR3 homolog B 2 2
MIRT605054 Arhgef39 Rho guanine nucleotide exchange factor (GEF) 39 1 1
MIRT605412 T2 brachyury 2 2 2
MIRT605550 Ppp1r16b protein phosphatase 1, regulatory (inhibitor) subunit 16B 2 2
MIRT606407 Glrx2 glutaredoxin 2 (thioltransferase) 2 2

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