miRTarBase - #MIRT581675


—
pre-miRNA Information
pre-miRNA mmu-mir-883b   
Genomic Coordinates chrX: 66789890 - 66789967
Description Mus musculus miR-883b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-883b-5p
Sequence 11| UACUGAGAAUGGGUAGCAGUCA |32
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Ppp2r2c   
Synonyms 6330548O06Rik, IMYPNO, IMYPNO1, PR52
Description protein phosphatase 2, regulatory subunit B, gamma
Transcript NM_172994   
Expression
Putative miRNA Targets on Ppp2r2c
3'UTR of Ppp2r2c
(miRNA target sites are highlighted)		 
>Ppp2r2c|NM_172994|3'UTR
   1 CTGGCCTCAGTGGCCCTCCCGGCCACCTCTGTAAGAGGACAGGATGAGTGTGGTGGCCATTTGTGGGGACATGAGTCCCA
  81 AGGCTGGAGTTGGCTTGGATGGTAATTGCCAGTTTAAACAAAGGAAAGGGGGAAGCCCCAGTTACAGGCGGATAAAGCAG
 161 ATGTGCCCGGTCCAGGGCTCCTTTGTTTACGGCCCTCCTGTCTCTGGCTGGGCCTTTCATTATTATCAGGACTTTATTCA
 241 TGTCCAGGGTGGGTCAGTTCCTATCAGGGAAAGTCAAGCTGGTCCATGATGGGGCTCCAGAGAAAGTTCATAGGGCAGTC
 321 AGGATCTGCAGATCCTCAAGGGAGCCAGCCAGCCATGTGCGCTGGCTCAGAGCTGCTGCTTCATGTTGGATTTTGGACAG
 401 AATTCTCACTGAGTGCTGGTTGTGGCAGCTTGTCACTTGGGTGTTTGGAATGTCTTTCGGTATTCTAGGTGCCAGGCCTG
 481 GGCAGGTTCTGAGAAGCAGAGCCAAAGTCGTAGGGTTAATCGTGAGGCAGGGCCGGGTCCCTCGGGTGGGTTTTTCCAGG
 561 CTAAGGCAAGTCCTGAGAAGCAGGACTGGGGACCCTGTGTGGCTGTGGCCCTCACCTCACTGCCGTCCGTTTGTGAGCCC
 641 ACTGCACGCTGGACATGGTGCTCAGCCCATGGTGGCTGTGGCTGGATCCCCGCAGCCCACTTTGTGATGGCTTTGCTGTC
 721 ACCCTTCCATGCTAGCCAGTCATCTTCCCCATGGCCGACAGCCGGTCCTGTCCCTGACTGTAGAATCCTCATGCCCACGA
 801 GACAGGTGTCCGCTCATTTCCATAACTCCCAGCTGAGATCCAGGCTCTGCTGGAGCTGGGGTTAGTAACCGTCCCCTCTG
 881 AATACAGGAGGGAACCAAGGCTCTGAGCAGAGAAGTGGGGTGCCTAGGGAGTCATACCCCGAGGGCTGAGCTGGTGTCCC
 961 ATTTCCAACCCGCCACACACGGGTGACTGGTTTTCAAGCAGCATTCCAAGGGAAGGTTTTAGAAGGGAAACATTTCCACG
1041 CCAGGCATTCAGTGCAGGCGAGCTGGTGACAAGATGTCACAAGAGAGAGAAGATGGAGAAGAGTGGCATCCTTGCTTGCC
1121 TCTCAAGATGCACGTCACCGACACTGTACCTGCAGCTTTCTCCTCACCCTTCCGGCTGCTCCCCAAGGAGGTTGCATGCA
1201 GCCAGGCAGCCCCAGAGGACGTTTTCTCAGACCTCCTCAGGGCCACAGGTGAGTGGCCATTCATTTCATAGTGACCCCTA
1281 CTCCATCCTCATTCCTGAAATCCCAGAACTGCAGGGCTTGGCGTTTTGACCCTGGAAGCAGAGAGCAGAGGGGCTTTGGT
1361 TTTTACAACATGGACTGCCACCCAGGAAACAGATGTGATGGAGTTTTTTTTCTCTCTCTCTCTCTCTCAGTGGATCAAGG
1441 TATCCTTGTGTGTGTCAAAGCACAAACCGCATGCACACTCACTTCTGGCCATGTATTTACACAGAAACTGGGTCCATTAC
1521 ATTTTTTGGCTTTTTTTTTCCTAAGTCAACGTGAATGAAGAATGTTTATCACTGTAAAATTCAGGCGCTCTGGACAGTAT
1601 TATATGTACATATTGACCCTGATTCAAGCTAGCAATTAAAAGATCGGCGACTTTCCCCTCTTGTAAAGAAGTTGAAGAAT
1681 GTGGCCAGTCGTATTTAATCCCATTGAAGCCAAAATCCTGGTGAGAGGGCCTTACACACTGGCCTGGATCTCTGGCACAC
1761 CCATCTGCCTTTGCGGTTTGGCGTTAGAGAAGATTCGCCTTGGAGGCTGGAGGCTGCTATGAGGTCATAGGTTTCACACA
1841 TAATGTCTTCAAAGTCAAATCTTACACGGTCTTCAAAAGGAGGGGTCTTGCACATGGCCCTAGTTTCCAGACTTCTGTTT
1921 GAAATGTCGGGTATCAAAGTAGAAAGGAAAAAGAGAGACAGAAAAAGATCTCAGAAGAAAAAAAAATAAGTCTTTGGGAC
2001 CCGAAGAAATCACCCAGGTTCAGAGCGGACTGCTAATAATAATGTGTGTATTGATCGAGGAAAAAGTGCGGAGGGGGGAG
2081 GGGCGGTGTGAAAAATGGCAGAAGGACCAGAGCCTTGCAAGCCACAGACTTTGCCACTTTACTCTGCTTAAGTTCTCTGG
2161 TCTTGAGTACGTGGCATTTCCCATAATCATTACGACATTGTTGGGGTTTTGAGTTTGTTTTCTTCTGCTTTGATTCTTCT
2241 CTGAGGGTGACGCCCTTGGAGGGTGTTGGGTATCTACGTGCTGGTAACATAGTCAGGCCCAGTCACTTGGGGTTCTGTGG
2321 CTCATAGGTGCATTCTTTTTTTTCTTCGGTGTCTCACAAATTATCCAACTGTTGAATAAAATTATAAATATGTTAATACC
2401 AGCTTTTTCCAATAAAATAACAAATGTTACATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' acuGACGAUGGGUA-AGAGUCAu 5'
             || || :|: | ||||||| 
Target 5' tctCT-CTCTCTCTCTCTCAGTg 3'
 1411 - 1432 150.00 -14.60
2 
miRNA  3' acugacgaUGGGUAAGAGUCau 5'
                  || : |||||||  
Target 5' cccagaggACGTTTTCTCAGac 3'
 1211 - 1232 130.00 -10.30
3 
miRNA  3' acugacgauggguaAGAGUCau 5'
                        ||||||  
Target 5' gagacagaaaaagaTCTCAGaa 3'
 1955 - 1976 120.00 -8.06
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' acuGACGAUGGGUAAGAGucau 5'
             :| :| |:|  ||||    
Target 5' guuUUUUUUCUCUCUCUCucuc 3'
 		3 - 24
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM1385343
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_DM_Ago2_CLIP-Seq_myotubes
Location of target site NM_172994 | 3UTR | GAGUUUUUUUUCUCUCUCUCUCUCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
13 mmu-miR-883b-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT426296 Scn2a1 sodium channel, voltage-gated, type II, alpha 2 2
MIRT579169 Cd93 CD93 antigen 2 4
MIRT579383 Ankra2 ankyrin repeat, family A (RFXANK-like), 2 2 2
MIRT581675 Ppp2r2c protein phosphatase 2, regulatory subunit B, gamma 2 2
MIRT582481 Mdga2 MAM domain containing glycosylphosphatidylinositol anchor 2 2 2
MIRT584649 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 2 2
MIRT587948 Asph aspartate-beta-hydroxylase 2 4
MIRT591263 Lars2 leucyl-tRNA synthetase, mitochondrial 2 4
MIRT596076 Ywhag tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 2 2
MIRT597944 Orc4 origin recognition complex, subunit 4 2 2
MIRT598809 Gm4951 predicted gene 4951 2 2
MIRT603477 Rbm8a RNA binding motif protein 8a 2 2
MIRT606744 Ryr2 ryanodine receptor 2, cardiac 2 2

Error report submission