miRTarBase - #MIRT582546


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pre-miRNA Information
pre-miRNA mmu-mir-326   
Genomic Coordinates chr7: 99552269 - 99552363
Synonyms Mirn326, mmu-mir-326, Mir326
Description Mus musculus miR-326 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-326-3p
Sequence 60| CCUCUGGGCCCUUCCUCCAGU |80
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Map3k7   
Synonyms B430101B05, C87327, Tak1
Description mitogen-activated protein kinase kinase kinase 7
Transcript NM_009316   
Other Transcripts NM_172688   
Expression
Putative miRNA Targets on Map3k7
3'UTR of Map3k7
(miRNA target sites are highlighted)		 
>Map3k7|NM_009316|3'UTR
   1 TTCTCTGGGACCGTTACGTTTTAAAATATGCAAAGACCTTTTTTTAAGAGAAGACAAACCATTATAACAGTTCATGAGTG
  81 TTAGCTTTTTGGCGTGTTCTGAATGCCAAATGCCTCTCTTTGCTGCATTTGTTATGTCAGTTACCTTTCTTCTTATGGTG
 161 GATATAAAATCCACTGTCGTGTTGCAGCAGATGATGGCACCTGTGGCTTGGGAAGGCGAGCGTGCTCAGCTTCAGAGCAC
 241 ATGAAGTGAACCTGGCTGTATGTGCATGCTCCTGGAGTGAGCTACCTAACAGGAGGGGGTAGCACACTGGCTACTGTGTG
 321 CAGGCATCATCCTTTCTCTGTAGTAAAAGGTGGGACCTCAAGAATTTTCTTCAAAGTGCTCATCTCAAAAATCTGATTTT
 401 TTTCCCAGTAGATGGTATGCTCCAATGTAAAGACAGGGTATTAAAATAACTTGTGGTACATTACAGAGGGACAGAATGTT
 481 GAGGCTGAGTTCAAAGACAGGGTTTGTGCCAACACATCCTGGATCAATACTAACTGTAGTCCCACCTGTTACTATTAAAG
 561 GTGAGTGGTGGCAGCTGTGCTTAAGGAGAGCAAGTTTACTGACTTTCGTGAGGTTGAGCATCGTCTGTGAAATATGAATG
 641 ACATGCTCTTTTTACACCCTATGGATTTTCAATTCTGTGTGAAAACAGAAGCCTTTCTGACTTTTAATATTAGTAGCAGA
 721 TGAAATTGAAAGATGATGATGTAGCTTAAATGATCCTTCTTGTAACCATTACTGAGTTGTTTTATGATATATTTGAAGAA
 801 AATTGAAAAATAATAGAGACACAAATCATTGGCCCAGCTAAAGGAGGCTTGAGCTGAGCTATCGGTCTGTTATACCAAAT
 881 AAGGAACACAGCAGGCTAATCAGGAGGCCCGAGTAAGGAAGGCAAAGGAGGAAAATTAAACCCACTTTCGTAGCTACTCA
 961 GAGGACTTGATTCCTCTGAAGTCCTTTCCTGAGTCTGTTGCCTTCCCATGCTTTTAAGAGAATTTGAACACTTACAAAGA
1041 GAATATTTATATTGTTAACAATAAGTTTACTACCAACCTTCAAAATAACTTAAAGGTAATAATAAAATGCCATTAAAAAA
1121 ATAAATCCCTAAGATCTATTTTTTCAGTCCATGTTAACACATTTTGATACCAGTGCATGAATGTCTTTTAAATTAGATGC
1201 CATCAGCTTCGCTTTTATAGTGTTCATGTTTGTAAAGAGAACATTTGGAATGGAAAGATGATCGCTGTGGTACTTAGATG
1281 TACATCTCGCATGTTCCTGCTCTGTTCCAGATGAGCACGAGAAGCTAGCGAAGGTGTTCTTTAAGGCTAAGACAGATAAC
1361 CTTTTTCCTGTTATTTTTTTCCTCTCTGAGTTTTATAATGAATCCCTTCTGTATACAGTGAAGGTAAGGGAGAGTTTATA
1441 GCAAGATGTGCCTTCCTCAAAACCTGTTAGTAGAAAGCTCCTGGGAGCTAAATGTCTTCTTTCACTTAAATAAAAAAGAT
1521 TATAGATAGCCTCAAACTCTATTAGGTGACATGAGTGCCGAGCTCAGCGTTAGTAGTGACATAAAACTGACACAGAAGAT
1601 AGCTGTAGTCTGAGTCCAAGACCAACACCGCCTGCCACTGCTGCCTGTGCAGACATCTGGCACTCCACAGGAGAGCCATG
1681 GTCTCTGCTGTGCAGCATGCTGAAAGCATCTTGACTTCCCAGGAGATTCCACATTACATGGATGCAAGCTCATTTCTAGC
1761 CAGTTGTTTTGCAAGGTCTGCATCCTACAACTAAAAGGAAATTTTGTTGTCCTGGTCACTTGAAAGTATTCAAACAAGCA
1841 AATGGAAGAAAACACTGGATTTGCTCATGGTACCAGTGTTGAAAGATTGTTACTCGGTTGCTTGTGTATGCTCAGCTCCT
1921 TGGGGTTTCCGACACTGTGGTCCTGGACAGCCTTAAGTAGGTGCACTGGCACACTCACATGCATTTTTAAAGCTCCACTA
2001 TCGCTGAACGTGAGCACACCATCCATACTTGAAATCCATACCAGTTTGCTCAATGTCTCAACCTGTAAAACCTTCAAATA
2081 ACAACATACCAAGGAGCCAGATGGTCACTAATTCCTGTTCTACAATTTATTCAAGCCTCACTTCTTGGGTGAAGTAGAAA
2161 ACAGGCAGACAGGTTTATACATTGATGTCCAAGAACCAGACAGTCCTGCACAGACACTAGTGGCATTTTGTTTCCATTTC
2241 CAGGAAAGGAAGCAAGGTTGTGCAATGTGGAGTGAAGTCCTCCTCAGCAGTAAAGCCATGCTCTAGGTTGAGTAAGTCAG
2321 TTAAGAGCACCATACTGCCATACTGAAAACATCTGCTTAGAGTTATTTACAAGTGGTTGGCTATTTTTTGTTGAAGTATT
2401 TTTTGAATAGCGAATGTCTTTTTCCTTCAAATTTCTGTCATTCTCCCAAGGCCCCCTCCTCCTGCAGCCAGAGATTACAG
2481 GTGGTTTTGTTTCTCCCTCCTTCCCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGTC
2561 AACTCCTCTATTCACAAGCTAGTAAAGCTATCTACTTTTATTCCTGTCCAGGTATTTCTTGTAAGATGATATAATGCAGA
2641 AATTCTTGTGCATACTCTGTAACTGAAGGAGGCTTTTTTGATTTATTTTTGTCTTTAATAAAATTCAGATTGTTATTTCA
2721 GGCTGGTATGTAGGAATGGTAGCTGGCTTCTCTACACGACGACTGAGCTGTGGGTTTGCCCAGTGGTATTTACCTCATGC
2801 AGACCAATAGCTGAATCACCAGTTTCCTAGCATACTCAGTACAGTGTACCATAGTCATGCCAGAGACTGAATCATCGGAC
2881 TGAGAGTCGGCTTAGCAGTTAAGAGCACTCATGTGGAGGACTAGGGTTCTGTTCCCAGCACCCGCATGGAGACTCCCAAC
2961 TCTCTGCAACTCCAGTTCCGGGGTGGTCTGACTCCTTATGTTCCTGATGGTTCTTGTAAGCACATGGTGCATATATACTC
3041 AGACGAGCGTGCGCGCGCGCACACACACACACACACACACACACACACGTGAGAAAAAAAAAGTCCTCTTTATTTTTTCA
3121 TGGGCCTTCATTTTTATGGAGTACAGCATAAAAGTGACTGACTTATCATTGCAAACTTCTTAATGTTTTTTCTAAGCTAC
3201 AGTAACCATTTTGCTTTTATATCAAGTAACCCCCCCCCCCGCTTATAGATAACCACATATTTTTACTATCTATCCATTAG
3281 TTTCCTCGTTGCTTCAGTATTGATAAGCTACTGTTACTAATGACAAGTTCATGTCAGCCCCACCATAGAAGCCCCTGTAA
3361 GATGCTGAAGGCGGGGGTTGCTAAGCATTGGCCCCTGAATCTGCTCTTCTACCAGCTCCATCTGTGTAGGTGGAGTCACT
3441 TGTGTGAGAACTGCCCACTCATCAGCAGAGGAAACATTTCTACACCTCCTCAACCCAGATATCTGCTCGGAATGACTGCG
3521 ATGTTGTGAAGGCCCCCTGTGAATTGGAGGGCTTCAGTGTTGGCACTCACTCACTTGGAATTCCTTCTTCCATCTGAGAG
3601 ACAAAATGCCCAAAATGAAGTACAAGTACTGGGCCAACTGACAGAAGATCCACCCGGCACACAGGAAGTGAGCTCTGGTC
3681 TGAACTTTTGTCATCTTTGTGGTGGTGGTATTTTTGTTTCTGTCTACTAGGAAGTCATATTTAATTAAGGCGTAATTTAT
3761 GTACAATAAACTTCATTCATTTCAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugACCUCCU--UCCC-------GGGUCucc 5'
            |||||||  ||||       |||||   
Target 5' tgTGGAGGACTAGGGTTCTGTTCCCAGcac 3'
 2912 - 2941 125.00 -22.43
2 
miRNA  3' ugaccuccuucccGGGUCUCc 5'
                       |:||||| 
Target 5' actttcgtagctaCTCAGAGg 3'
 944 - 964 124.00 -13.70
3 
miRNA  3' ugaccuccuucccGGGUCUcc 5'
                       ||||||  
Target 5' tacacctcctcaaCCCAGAta 3'
 3481 - 3501 120.00 -8.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
23 mmu-miR-326-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004432 Smo smoothened, frizzled class receptor 3 1
MIRT005596 Ets1 E26 avian leukemia oncogene 1, 5' domain 3 1
MIRT581306 Rnf150 ring finger protein 150 1 2
MIRT582546 Map3k7 mitogen-activated protein kinase kinase kinase 7 1 1
MIRT582559 Maff v-maf musculoaponeurotic fibrosarcoma oncogene family, protein F (avian) 1 1
MIRT582898 Ildr2 immunoglobulin-like domain containing receptor 2 1 1
MIRT583744 Ephb3 Eph receptor B3 1 5
MIRT590217 Dync1li2 dynein, cytoplasmic 1 light intermediate chain 2 1 2
MIRT590852 Vsx1 visual system homeobox 1 1 2
MIRT593069 Ccdc25 coiled-coil domain containing 25 1 1
MIRT596778 Usp22 ubiquitin specific peptidase 22 1 1
MIRT596972 Tmem179 transmembrane protein 179 1 1
MIRT597063 Tbx5 T-box 5 1 1
MIRT597081 Tars2 threonyl-tRNA synthetase 2, mitochondrial (putative) 1 1
MIRT598144 Mylip myosin regulatory light chain interacting protein 1 1
MIRT600897 Fbxl16 F-box and leucine-rich repeat protein 16 1 1
MIRT600919 Exoc8 exocyst complex component 8 1 1
MIRT604218 Clgn calmegin 1 1
MIRT604799 Phactr1 phosphatase and actin regulator 1 1 1
MIRT605009 Ept1 selenoprotein I 1 1
MIRT605050 Efr3b EFR3 homolog B 1 1
MIRT755982 Th tyrosine hydroxylase 3 1
MIRT756011 Tlr4 toll-like receptor 4 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-326 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-326 Propranolol approved 4946 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-326 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 up-regulated
miR-326 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-326 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated

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