miRTarBase - #MIRT582893


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pre-miRNA Information
pre-miRNA mmu-mir-186   
Genomic Coordinates chr3: 157544279 - 157544349
Synonyms Mirn186, mmu-mir-186, Mir186
Description Mus musculus miR-186 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-186-5p
Sequence 7| CAAAGAAUUCUCCUUUUGGGCU |28
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Ildr2   
Synonyms 2810478N18Rik, 3110063L10Rik, AI852300, D1Ertd471e, Dbsm1, Ll
Description immunoglobulin-like domain containing receptor 2
Transcript NM_001164528   
Expression
Putative miRNA Targets on Ildr2
3'UTR of Ildr2
(miRNA target sites are highlighted)		 
>Ildr2|NM_001164528|3'UTR
   1 TACTTATAAGACACCTCTCTGGATGACTGGAAATCAGATGCAGACTATGGAGACAAGACCCAAATCTGAGAGCCGGCAAG
  81 CCTAGGATCTTCTCTGGCCAGCAACCACCTTGGAAGCTTTGCTGATCTCTGCTTTGGCAAGGGATCCTCCTTTAAGAAGG
 161 CTGATTTCAAATCTTAGTGCCCATCTATCTCGAGCAACTTACCAAGAAAACGCTCTGTGAGAACATATCACGTAATAACC
 241 GACCAAGTTTATCTTACACCCCCACCCCCACCCCCATTTCCTTAGCAGAAACAAGACTCTGCGTCCAGTTCTGAAGCTGG
 321 AAGCTTTGAACCCCTGATCTCTAGAAATTACCTATGCCTGCAGTATGTTTTTCTACGAGTGCTATTCTGTGCTTAGACAG
 401 AGGAATTTACTACTACAGTTAGAAGACCGTCTGCTCACAAGAGAGATAAATGGTAAAATGGACCTTGTATCCCCTTGCTT
 481 CCAGTCACTGGTCAATGAGTCTTGTTATGCTAAAACCAGAAGGCCTTTAGTGAGCGTACTGGCCGTGACCTCCTGGGCAA
 561 TCACAGAAATGGCTTCAATTTGCTGCTCTGACTCACAATTCTAAGTGGCTGGGACAAACAGAGGAGAGCATTTTGAAAAA
 641 CCATCTTAAGTGGTCTTTCTTTTTCCATTCAGAGGACACAAACTGCTTTTCATCTTTCTGTCGAACAGAGTGACAATCCT
 721 AAGGTTCTCCCTGCCCAGCCCACACTGGTCCCTCTCTTCCCTCCCTCTCCTGGTCTTTTCAGGGCTGGTGCCTCTGAGGG
 801 TGTTCCACTCCATGCTTCAGTGTGAATAGCTTGTCATCAGGTGCCTTTGACAGATGCTTCAAACAAACATTTGAGAGAGA
 881 AGAAAAGCAGAAGTCGGTGATACAAAATGAACAGGAAATGATATGTAGGCTCATTATATTTTGAATGTGGGTTGTTTCCC
 961 CACAAACACACTCAGATTTGTTTTTGTTTTTATTTTTGGATTTGTACTTCACTTAAGAATTATTTCTACCATCCTGATTC
1041 TGCAGCTGTTGGGCACCAGGGAATGTGGTGTCCACATCTTTTGGCCTCACTGGCCCACCACTATTGATGCTTTGGGGAAA
1121 AGAAGGACAGCACTTCCTCTTCCTGCCATTGCAAAAAAAAAAATTGATTTTTGCCTGAATCCCAAATTGAACTTTTGTAG
1201 GTAAACTGCAAAAGTGGCCACAAACTCTTCCCCTCTCATGTTCCTGTGAAGGGATTTGTCCTCTTGCTGCCACAGGCCCT
1281 GCCAAATGCACCTCAGCTATCCTACATGATGAGAGAAGAGCCTGGTCACCACCGTCATTATCTGTGCCCATCTTATCAAC
1361 TTTAAGCAGACTTGGAAGAACATCTAGCCACGACCAACAAAAGAACTGCCTAGCTGAGCCGAGCCCAAACTGGAGATTCC
1441 CGCTTGAGAGGAGACATTCAGCATTCCTGTGTTCATTTATCATTGACGATAAACCTCCCGTCAGAATATTTGTCTCTGGT
1521 CGGTTACTCACCCAACCTTGGGTGTCACACAACCTTCACTTTTGTTAGCAGACTTTTCAATCTGCATTATTGTGGTGAGA
1601 CACGTGACTGGATGAAGTGACTGGAGCAAGGGGATGCTTGCTATCCCCTAATCCAGTGGTGGTCTACTTCTACTTATTGA
1681 TCTACATGTAGTCTCTGATTCACTGGTCAGTATTTCCATGGCCACGTGACTGGAATTCCAGAGTCCATTCTGTTAGCATC
1761 CATTATACTTCATGAGATTTCCAGAAAAGGTCCTCTGTGAGTGGTGTAAGAGCTGCTGGGTTAGGGTGGGTGTTGGGGGG
1841 TGGAATCATTACTTGGAGGAGAACTGGCCTGCCAAAGGACTTCACGGTTGCTTTGGCCTGCCCTAGATGGATCAGGAGGA
1921 TACTTCAGCCCAATGCTGGCACTTCCAAGGGCTGGAAGACAAAAGCCATAACCCTTGTGCTGAGTTTTAGGTTTGTTAGT
2001 GTCCCTGGCCTCAGAACACCTAGGTCTGATCTGTCTGTTTGGGCTCTAAATCAATATGGCAAAAACATCATTTCTTAGTC
2081 ACCAGCTTTTGATTTCAACTTGCTCAGGCACTTTTGAAGAATATTGGATAGCCGCAGTAGCTATTGTTATACTGAGCACT
2161 GTGTCAGGCTTCTTAGCACCAAAGAGCCCCATAGCACAGGCTACAGAGACCAAATATATTGCTTTATAGAGCCAGGGGCG
2241 TGTATGAGCTTGGGGAAAGCTGAGGGATCGATGAGTGAAAGAAAAAAGTTAAAATTGGAAACATAAGGTTCTAAAGACAA
2321 CAAGTCTATAGGCTGACAAATTAAAAAAAATTTCAATGTAGAGAAGATAACAGGCTTTCAATATAACGGGGGAAAGTGGG
2401 GCACAGATTGTTCTTTATAGGGCATGAGTCACGTGGGCTTCCAGACCTTCAGTACAGAGGAAATTCAGTTGCTTCTGGGT
2481 CCGTGGATAGGAGATGATCTGAATGGACAAGGCTAAGCTGGCCGTCCTTGATGCCCTCGACATTTCTTTACACACCCCTT
2561 TGTTTCTTCTCCAAATACTGTGTCCTGTACAGGAAGTGCCTATGCGTATTAGTTCCTTTCCTGTTTTTCTAGGGCATAAG
2641 CAAAGTGTAAGAGGTGATCTCCATCCACTGATCCCCTACAATTTAAGAAGGAAGATAAGTCATGCCCAAGAAAGGATGAG
2721 TATATTTTATGCATATGATAAGAAATAGTGCTATGGATAAATTATAATAAACCCAGAGATTTAAAGTTTTCTTTAAAAAC
2801 AAAAACCTTAAATGGGAATATTTTGATATTTAAGTGTTGTGTGTTTGTCCATCCATCCCATTTTTAGGACATGCTCAGTG
2881 ATCTGCAAAGCCAGGCTGTAGAAGTCTGAGCTGAAAGGAGGTGAAGGAGAAGAGAGAGGGATGAGTGGCCTCAGGGAGGA
2961 GGGAAGAGAGTAGAGGCCCGCTTACAGGAGCTTCTGTCTCTGTCTGTGACTCACAGCTGAGTCAGGGACAAGCTGGAGGA
3041 GGGAGTATGGAAGCAGGTGGCAGGAGAGGTCCCCTGGTGCTCAGAGCTCTTCTCTAGGCTATGTATAGACTCATTAGGAG
3121 ACTCAGGACTGTATTCAGTTCTTCCATCCAAGCAAGCCCAGGGGAGCTTGGGATTTAGTCCTCCTGGCACTTGTATCTAC
3201 AGCTTGGGGTGCAGTAGCACCTCACATGGGTTGGGGACCTCACCTCCCTTCTCATGATCCTCACTCTGCATGTGGTGTAG
3281 GGGTGGGCACCCCAGGGTGAGAGGGGGCTGGCGCTACATATAAAAATCTGGTTAGATCCGAAGCAGTCTTTGAGAGGAGT
3361 GGAGTAACTAACAGACACCGCTTTGGCTCATCTGCTCTCCATCCATTTCTAAATAGATGGATAAGCCATCATCCACATTT
3441 ATGGAGTCACAAACCAGTCAGATCTTTAGATTCCCAATCTATAGGCCTTTCCTGCTGGATCTGTGTTTTTGCAAAATTGC
3521 CTAGTCATAAGAATTACTTGCCTAGGGACTGGAGAGAGATGGCTTAACAGTCAAAAACACTGCTTTAGCCGAGGACCCGA
3601 GTTTGGTTCCCAGCACTCTACTAATGCTCACAGCTGTCTGTAACTTCAATTCCAGGGACCCACTGATCTCATAGAGCATC
3681 TGGGAGCACTAAACTCACATGGTACGCATATATACCATCAAAAAACTCTCAGGCACACAAAATAAAAATAAATACATTGT
3761 TAAAAACTGAAAAAGAAAGGGGCTGGAGAGATGGCTCTGTGATTACGTGTGCTGGCTACTCTTCCAGAGGACCCACATTC
3841 ATTTTTCAGCACATGGAGACTCCCAGCACCAGTTCAGACACACACACACACACACACACACACACACACACACACGAAGG
3921 CAAAATACACACCTAACAAAGAAATAAAGCATTTAAAAATACTTGGTAAAACAATTACTTGCCTGGGGGACTGGGATGTG
4001 GCTCAGTTGGTAGAGCGCTTGATTAGCATGCACAAAGCCCTGGTTCAATCCCTAGCACAATAAACTAGGGGTAGTGGCAC
4081 ATGCCTGTGATCCCAACATCTGGGAGAGTTTCAAGTTCAAGGTCATCCTTGGCTACATAGTGAGTTCAAGGTCACCCTGG
4161 GCTGTATGATACTCTGCCTTAAAGAACCAAACACTGAAAATAACAATGAAAAAACACAAGGATTACTGACTGCCACTGTC
4241 ACAAATGCTGTTGGACTGTACCTTGGAGAATGGATGGGTGGATCTGGAGTAAGGATTTGTATTCTGAACATATCCTTTTA
4321 GAATGCCTTGTGGTAGATGCATTTGGGTGGTGCTATACTGGATCATACCTCTGGTGCACCCCTATCTGCTGGCCAGGAAT
4401 ATTGTTTGTGCTGTGGATTATTTCATTCCAATATCACTGTGAGGTCCCTCTAACTTCCTTAGGTCTGGCACTGGTGTGGC
4481 ATCCAGCAGTCCCAGCTATGACACTGGAGAATGGCTCACCAGAGTCAGGCTGAAAGGAAACATTTAAAGGGAGGGGGTTG
4561 GAGGACCTCCCCCCGGGAGGCTTCTTGACATGTTCCAACTCCCAGAATAGTGATATGTTGTGACAGGCTGAGATCAGACA
4641 ACAGGAATTACAGACAATTTTCTTATTCCCTTACCATCCTGAATAAAACTTAGCTCATGAATAGAAAAAAAAAAAAGCCA
4721 TCAGAGAAAATGGCAAACGTAAATCATTTTTAAAGGGTAAAAATTAAAAGCTTTGCTAATAACATAACTTTCATGCTAGG
4801 ACCAAAAGTGGGTGGAGAAAAAAATAGTAAAATATATATTACCTATTCCAAAACTGATTTAATTGCAGCCAGAATCTTAT
4881 GGAAGTTTAGAAGTGATGTATAGAGTACAGGAATCACCCATGGAAATTCTAAGGTCTTAGAAAGCAAAAGGTTCCCTACC
4961 AGGACCTACCCCCTAGTCACTTGGGATTACCTGTGAAGCTCAAAGGCCCTAGTGGCATCAAAGGTGAGTAAGAAGAAGCC
5041 GAGATGCTTTAAGCAACAGCGTGAGGTTGGCATCAACGGGGCACATTTGTTCTTCACAGCAAGCCCAGTGTTTTCCCATC
5121 TTACCCAATGTGGAGCTGGGTCTGAAAGTGTGCCAAGTGATCACCTATTGCCAAATAGCTTTTAGTCTTTAGATGGCCTT
5201 CTGACTGTCCAGGTCCTAAGCCTACAGTAATCACGGGCCCAGCCTCTAGTGTGTTCTCTTCCCAAGCAGGTGGATAGTGG
5281 AGAGAGCCCTGACTCAATATTCACTCACACATCATTGGTGAGGAGAAGCTAGGAAGGCAGGCATTTGCCACTTCATCTAT
5361 CCACAGGAGGTTCCTTGAAGTCTGCCCTGAGAAGGAGGTGTCTTTGCTGGGGAGGATCTTCATCATCAGCATCAAGCTGT
5441 GAGGGGAAAGGCTTTGACAAAAGGGTTGCCACTTTCTGAATTCTTCTCAAAGAGGAATTTCTAAGCCAAGCTACAGATTC
5521 ATCCAGGCTCAGAATTCCATGGCTGTGGGCAGGAGCTGTCATCTTCACTATATTTTGAGATACATTTTTTTTTAGGTAGA
5601 ACTCGAGGTCCAGATCTAGAGGGGGATAAGGACGATGAGAAGGATAAAGTTGTGGCAGTTGAGCTAAAAGTCATGTTCGA
5681 GTTTTTTGGTGGGTCTGACTGGACAGGGGAAAATGTGGTCCGACTTCTTTTATCTAAAAGTTTGGGAAAGATACCCATAG
5761 CTTCTCTCTTGCCATGTTTATTAACAAAGATGTTAGACACTACTCCATGAGAAATGTCCTTGTGAAAATAAAAACCATGC
5841 CATCAAAAGAGTCGGGTGCAAAGATGCCTACTTCATGAGAATCACCTGCCCAGTTGTTTTTGTGCCTTGTCTGTGACATC
5921 AAAACTGAAACACTTATATCACTGTCACTCATGGTTTTCTTTTCCTGTGTCATACATACAAAGTGCATTTGATTGTAATG
6001 ATTTAAAGTAAATAAAGCATTTCATCTACTTTTGTTA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ucggGUUUUCCU------------CUUAAGAAac 5'
              |||||||:            ||||||||  
Target 5' ttgaCAAAAGGGTTGCCACTTTCTGAATTCTTct 3'
 5454 - 5487 136.00 -13.30
2 
miRNA  3' ucgggUUU---UCC---UCU--UAAGAAAc 5'
               |||   :||   |||  :|||||| 
Target 5' gggggAAAGTGGGGCACAGATTGTTCTTTa 3'
 2388 - 2417 131.00 -7.80
3 
miRNA  3' ucGGGUUUUCCUCUUAAGAAAc 5'
            ||: ||| |:|||| :||| 
Target 5' aaCCTTAAATGGGAATATTTTg 3'
 2804 - 2825 128.00 -13.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
46 mmu-miR-186-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577608 Stxbp4 syntaxin binding protein 4 1 1
MIRT577884 Qpct glutaminyl-peptide cyclotransferase (glutaminyl cyclase) 1 1
MIRT578137 Noc3l NOC3 like DNA replication regulator 1 1
MIRT578249 Mnat1 menage a trois 1 1 1
MIRT579147 Ces2g carboxylesterase 2G 1 1
MIRT579219 Ccdc138 coiled-coil domain containing 138 1 1
MIRT579578 4933427D06Rik RIKEN cDNA 4933427D06 gene 1 1
MIRT580348 Tmem33 transmembrane protein 33 1 1
MIRT580592 Tbl1xr1 transducin (beta)-like 1X-linked receptor 1 1 1
MIRT580668 Stk32a serine/threonine kinase 32A 1 1
MIRT580744 Srek1 splicing regulatory glutamine/lysine-rich protein 1 1 1
MIRT580779 Sp6 trans-acting transcription factor 6 1 1
MIRT580847 Sltm SAFB-like, transcription modulator 1 1
MIRT580944 Slc26a5 solute carrier family 26, member 5 1 1
MIRT581214 Samd4 sterile alpha motif domain containing 4 1 1
MIRT581276 Rprd2 regulation of nuclear pre-mRNA domain containing 2 1 1
MIRT581520 Ptprb protein tyrosine phosphatase, receptor type, B 1 1
MIRT582203 Nfia nuclear factor I/A 1 1
MIRT582893 Ildr2 immunoglobulin-like domain containing receptor 2 1 1
MIRT582996 Id4 inhibitor of DNA binding 4 1 1
MIRT583079 Hnrnpa3 heterogeneous nuclear ribonucleoprotein A3 1 1
MIRT583125 Heca hdc homolog, cell cycle regulator 1 1
MIRT583739 Epm2aip1 EPM2A (laforin) interacting protein 1 1 1
MIRT584127 Crispld2 cysteine-rich secretory protein LCCL domain containing 2 1 1
MIRT584166 Cpox coproporphyrinogen oxidase 1 1
MIRT584531 C230081A13Rik pseudopodium-enriched atypical kinase 1 1 1
MIRT584646 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT587978 Armc1 armadillo repeat containing 1 1 1
MIRT588832 Sp1 trans-acting transcription factor 1 1 2
MIRT590758 Acbd5 acyl-Coenzyme A binding domain containing 5 1 1
MIRT593146 Mmp12 matrix metallopeptidase 12 1 4
MIRT593495 Gjc3 gap junction protein, gamma 3 1 1
MIRT594880 Lrig2 leucine-rich repeats and immunoglobulin-like domains 2 1 1
MIRT594898 Ifi204 interferon activated gene 204 1 1
MIRT594932 Gdi2 guanosine diphosphate (GDP) dissociation inhibitor 2 1 1
MIRT594945 Fmnl2 formin-like 2 1 1
MIRT595197 Emc3 ER membrane protein complex subunit 3 1 1
MIRT595343 Frmd4a FERM domain containing 4A 1 1
MIRT595402 Arsk arylsulfatase K 1 1
MIRT595430 A630033H20Rik RIKEN cDNA A630033H20 gene 1 1
MIRT595947 Nelfa negative elongation factor complex member A, Whsc2 1 1
MIRT596153 Opn5 opsin 5 1 1
MIRT601390 Utp23 UTP23 small subunit processome component 1 1
MIRT602153 Intu inturned planar cell polarity protein 1 1
MIRT602577 Colec12 collectin sub-family member 12 1 1
MIRT604817 Nr5a2 nuclear receptor subfamily 5, group A, member 2 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-186 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-186 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-186 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-186 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated
miR-186 Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 down-regulated

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