| miRNA Infomation | |
|---|---|
| miRNA name | mmu-miR-1194 |
| Gene Information | |
|---|---|
| Gene Symbol | Elf1 |
| Synonyms | Elf-1, Sts1, mElf-1, p70 |
| Description | E74-like factor 1 |
| Transcript | NM_007920 |
| Expression | |
| Putative miRNA Targets on Elf1 | |
| 3'UTR of Elf1 (miRNA target sites are highlighted) |
>Elf1|NM_007920|3'UTR 1 TACACCAAAGGAATTATGGGTGGGTATTTTTTTAATTGAACTCATTTTTCGGTTGTCTGACCATAACATGATTTTTTTCT 81 AAGATGAAATCCACAGAAGTTCTTAACTGTTAAAAAGTTACTTTTGACTTTGCTAGATAAGGAGGCGAACCCAAGTGTGT 161 GTCTCCTCTGAATGTCTCAGAACTCAGTTGTGAAGGAGCAGGAGGTTTAGACGATGAAGAAATTCTTGACTGTTTTTCAG 241 TTGCTATATCATATTTTAAAAGTTTCTTTTCTAGCTTGACACTGTACTAGCTTCCTGAATTGTTTGAAAATACTACTTAA 321 ATATAAGACAACAGGAAATTTATATAGGAAACATTTTCATTCCACTTGTATAATTTAAGTCTTAGCTCTTTCAGATGCAA 401 GAAACTTATACTATTAAAATTGTTGCGATGTCACTTAGCTTGACTTTAAAGTTGGCTGGACTGTTATACTATGAATATAC 481 TACGAATTTGTAAACTATCATTATAAATTGAAGTGTATATGCTCAGTATATTGGCATATATGTGTAATTCCAGCACTGGG 561 AGGAGGACAAAGGGCAATCAGGAGTTCAAGGCCAGCCTTAGCTATATAGTGAGTTTGAAGCCAGCCTGGGCTACATGTAA 641 CCTTCCCAAAAAAATCCAAAACAACAACAAAAATATTTAAGTGTGCCTATGATAATGTCTGAACCTGAGTTTAAGAAATG 721 TGATGTAGCCGGGCGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGAGGCAGACGCAGGTGAATTTCTGAGTTTGAGG 801 CCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAAACCCTGTCTCAAAACAAACAAACAAATAAAA 881 AAAGAAATGTGATATTATAAAGGCAACACTTGTGACACTTCCACTAGCTAGGTGTGTAACCCTGGGTAGGCCACTCCATC 961 TCCTAGGTGTGTAACCCTGGACAGGACATTTCACCTGCTAGTCTGTGCTATTCTGGTAAAATGAAGGGATGCATTGGACT 1041 AGACAAAATGATCTAAAAGTTTCCTTATTATAAATTTTAGGATTTTATTATCTGTACTTTAAAATTATTTCAAATATTAC 1121 CAGATTATCTCCTTATGTAAAGGTAAAATACTGCTTAAAAATATTTTCCTCACAAAATTACTATCAGGTCTGCCATAAAT 1201 TCTTCCTAAGTTCTCTGCTCATTAATCTTCAGACCACAGTTACTAAAACTGTGGCTTAGTAGTAAGCTGAGGGCTAAATG 1281 AGAGACTGCTATTCTAAGAGCATCAGGGCTCTGAGGGCCAAAGAAGCAATGGGAGTCCTGAGCTAGTATAGCTGTGTCAC 1361 TTATTATAAAAGCATAACTGTTTTTTATTTGGATCATTTTTTACTGTGGGTGAAGGGGAGGGGAAGTTGCATACAAAGAT 1441 ATATATCCAAGTTTCCGAAGTAGAGTTTTTAGATTACTTTTTCAATGTAAATAATGTATATTTAATGTCACAAGAAATTG 1521 TTTTCTGCAAATTCTCTAGTATAGAAAGTTTTGTAGAAGAAAAATTATTGTGTTTAGGGGTATAATATTATATGTTTTCA 1601 TATTATGAAATGAATTTGTATTTAAACAAAAATTTAAATTTTGGAATCCTCTAAACATTTTTGTATCTTTAATTGTTATT 1681 ATTAAATCATGTAAAAGTTCTCAATATTTCTGTTTTCAAGC Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
| Experimental Support 1 for Functional miRNA-Target Interaction | |
|---|---|
| miRNA:Target | ---- |
| Validation Method |
|
| Conditions | C2C12 |
| Location of target site | 3'UTR |
| Tools used in this research | TargetScan , miRTarCLIP , Piranha |
| Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes
... - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell. |
| Article |
- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al. - Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
|
| CLIP-seq Support 1 for dataset GSM1385343 | |
|---|---|
| Method / RBP | HITS-CLIP / AGO2 |
| Cell line / Condition | C2C12 / C2C12_DM_Ago2_CLIP-Seq_myotubes |
| Location of target site | NM_007920 | 3UTR | GGGUAUUUUUUUAAUUGAA |
| Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
| Article / Accession Series | PMID: 25083871 / GSE57596 |
| CLIP-seq Viewer | Link |
39 mmu-miR-1194 Target Genes:
Functional analysis:
ID |
Target | Description | Validation methods |
 |
 |
|||||||
| Strong evidence | Less strong evidence | |||||||||||
 |
 |
 |
 |
 |
 |
 |
 |
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| MIRT577399 | Usp29 | ubiquitin specific peptidase 29 |  |
|
2 | 2 | ||||||
| MIRT578645 | Gtf2h2 | general transcription factor II H, polypeptide 2 | |
|
2 | 4 | ||||||
| MIRT583791 | Elf1 | E74-like factor 1 | |
|
2 | 2 | ||||||
| MIRT585587 | Tox4 | TOX high mobility group box family member 4 | |
|
2 | 4 | ||||||
| MIRT585769 | Spg21 | SPG21, maspardin | |
|
2 | 2 | ||||||
| MIRT586369 | Pctp | phosphatidylcholine transfer protein | |
|
2 | 6 | ||||||
| MIRT586934 | H2-T24 | histocompatibility 2, T region locus 24 | |
|
2 | 4 | ||||||
| MIRT587278 | Fam154b | stabilizer of axonemal microtubules 2 | |
|
2 | 2 | ||||||
| MIRT589358 | Osbpl8 | oxysterol binding protein-like 8 | |
|
2 | 4 | ||||||
| MIRT591418 | Cd300a | CD300A molecule | |
|
2 | 4 | ||||||
| MIRT594881 | Lpar6 | lysophosphatidic acid receptor 6 | |
|
2 | 2 | ||||||
| MIRT594899 | Hs6st2 | heparan sulfate 6-O-sulfotransferase 2 | |
|
2 | 2 | ||||||
| MIRT595087 | Unc5d | unc-5 netrin receptor D | |
|
2 | 2 | ||||||
| MIRT595608 | Jazf1 | JAZF zinc finger 1 | |
|
2 | 2 | ||||||
| MIRT595914 | Syne2 | spectrin repeat containing, nuclear envelope 2 | |
|
2 | 2 | ||||||
| MIRT596713 | Ybey | ybeY metallopeptidase | |
|
2 | 2 | ||||||
| MIRT597013 | Thoc6 | THO complex 6 | |
|
2 | 2 | ||||||
| MIRT597522 | Rbak | RB-associated KRAB zinc finger | |
|
2 | 2 | ||||||
| MIRT598050 | Nip7 | NIP7, nucleolar pre-rRNA processing protein | |
|
2 | 2 | ||||||
| MIRT598082 | Neu3 | neuraminidase 3 | |
|
2 | 2 | ||||||
| MIRT598528 | Itsn1 | intersectin 1 (SH3 domain protein 1A) | |
|
2 | 2 | ||||||
| MIRT598953 | Ggps1 | geranylgeranyl diphosphate synthase 1 | |
|
2 | 2 | ||||||
| MIRT598967 | Gata5 | GATA binding protein 5 | |
|
2 | 2 | ||||||
| MIRT599086 | Efcab4a | calcium release activated channel regulator 2B | |
|
2 | 2 | ||||||
| MIRT599348 | Commd7 | COMM domain containing 7 | |
|
2 | 2 | ||||||
| MIRT599554 | C3ar1 | complement component 3a receptor 1 | |
|
2 | 2 | ||||||
| MIRT600166 | Xpo7 | exportin 7 | |
|
2 | 2 | ||||||
| MIRT600668 | March5 | membrane-associated ring finger (C3HC4) 5 | |
|
2 | 2 | ||||||
| MIRT600971 | Dlc1 | deleted in liver cancer 1 | |
|
2 | 2 | ||||||
| MIRT601455 | Tnfsf10 | tumor necrosis factor (ligand) superfamily, member 10 | |
|
2 | 2 | ||||||
| MIRT601502 | Tmem88b | transmembrane protein 88B | |
|
2 | 2 | ||||||
| MIRT601514 | Tmem151a | transmembrane protein 151A | |
|
2 | 2 | ||||||
| MIRT601558 | T2 | brachyury 2 | |
|
2 | 2 | ||||||
| MIRT601925 | Onecut3 | one cut domain, family member 3 | |
|
2 | 2 | ||||||
| MIRT601984 | Mzt1 | mitotic spindle organizing protein 1 | |
|
2 | 2 | ||||||
| MIRT602669 | Car5b | carbonic anhydrase 5b, mitochondrial | |
|
2 | 2 | ||||||
| MIRT603331 | Slc35d2 | solute carrier family 35, member D2 | |
|
2 | 2 | ||||||
| MIRT605546 | Ppp1r16b | protein phosphatase 1, regulatory (inhibitor) subunit 16B | |
|
2 | 2 | ||||||
| MIRT606291 | Nrcam | neuronal cell adhesion molecule | |
|
2 | 2 | ||||||
| miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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