miRTarBase - #MIRT584578


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pre-miRNA Information
pre-miRNA mmu-mir-203   
Genomic Coordinates chr12: 112130880 - 112130955
Synonyms Mirn203, mmu-mir-203, Mir203
Description Mus musculus miR-203 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-203-3p
Sequence 48| GUGAAAUGUUUAGGACCACUAG |69
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Bnc2   
Synonyms 5031434M05Rik, 8430420F16Rik
Description basonuclin 2
Transcript NM_172870   
Expression
Putative miRNA Targets on Bnc2
3'UTR of Bnc2
(miRNA target sites are highlighted)		 
>Bnc2|NM_172870|3'UTR
   1 TCCCAGAACGGACACTCCAAATGCCAGCTCTCACCAGATGGCCTACATGTTTGAACTGCCATAGCCAGTGTGTGCTTCCG
  81 CAGTGGGGCACATATGTGTGTGTACATATGTGTGCCTCTGTGTCTACACTTGTGCACACACATACTTTCTTTTCTTTAGA
 161 TAAAAATGATAAATACTAGGTGCTTTTGAAATTTTTTTCTTTTTCCTTTATAGTTTTGGGAGAGGGTGGATCTTTTACTT
 241 GGGGTAAAAACAAGAGTGCCCTTTTCAGCACACACACACACACACACACACACACACAAACACAACATAACACACAAAGT
 321 GTGCAACTAACCCCAATTTTGATAGGATAATTCTTGGTCTTCTCCAAAGAGACATTTTGTTGTACCTATGACTGTTGCCT
 401 GCAAAAACAATAAATAAATAAAAGGGGAAAAAAGAGAAAAGAAACAAAAAGGAACTTCTTACAGTTGTCTTTTGTGAACT
 481 TTAGGGTTTTGAGAGAGTCTTTAGTTAAAGCATGGCAGATTCACCTGTAAATATTTAGCCTTGAGAGGCCAATGATGTAA
 561 AGCAATTGTATTGATGGTTCTTTAACTCTCCTTTAATTTTGGCAGTTACATCCAGCTGTTAGATATGCAGGAAATAAATA
 641 GTTTGCTGGCTAGTTCTATTCATTTTTGTTAGCATTAATGCACATTTTCTTTAAAAAAAAAAACAACAAAAAAACAAAAA
 721 ACAAAACATGGTCTTGTTTTTCCTAACCTGTTAGATGTAGTGGTAAAGCTGTGCTGGCATGCTTCACAGCACAGGTCTGA
 801 TTTCTTAAAAATGTCCTGTACCAGTACGTAAATCCCATCATGCACTTTGTTCGTACGCTAAAGGGGATGTGTAATACCCA
 881 GGCTTCCTTTTACCCAGAAACTACCGCCATACTTCAAGGAGCATCTTTCTTTTAAAAAACAACCCACTGTATGAAAATGG
 961 TCTGGTCAGTAAAGGGCACAAATGCCCAACAAAAAGAGTATTAGTGTTAACACAAAACTGCCAACTTTGCACAAGTTTTC
1041 AGAAAAGAAAATACAATTAGTCACTCAACATAACCACATGCCAATTCTTTGGCCACCAGAAAACCGCTTAAACACACACA
1121 CGCACACGCACACACGGTGATCCTTTTGAGTGCCGAATGTTATTAGAATCAACATTACATCCTTCCTCTATACACGTTAA
1201 GTCCCAGAAAACAAAATACCGCGGTGTGAAAAAGCCAAGGTGTTCCTTTGGTAGAGGCAGGATAATATTTCGGGCACAGT
1281 AGCCCAGATTGCTCACTAAAGGAACAAAGCTGCCTACAGTAGGAGACTGGACTCACTTCCAAGGCCCCAACCCTTATCCT
1361 TTGAAAAGAAAGAAAGTCCGAGCTGATTGGTCTCTTGTTTGTCACAGTAGCACTGCCAGTCAGGCCTGCTTAATAGAGGA
1441 AATCAAAGTTCCCAGCAGTTCTTCATGGAAGCTCTGAATGCCTGTGCTGACCTTGAGCTGAGCAGCCCCCTTCTCCAGGG
1521 GCAGAAGACAGGGTCTCCCTGCGTAGTAGTGTTTTGTGTTTTTCCCCATGCAAATTTGTCAGAGAGAAATGAGAGCAAGC
1601 CTGAAACCAAGCAGTGGAGAGTGAGGACAAGGGTGGGTGGGGGACACTGCTCTGAACCTTCTTGTTTTGTTTTGTTTCTG
1681 ATGTTTACACATGTTGCCTGAGCTGGTTAACCACATCAGCAGCCTCAGCTACCACAGCATACTGACTAAGTGAGGATATT
1761 TACTCAACCTCATAGGTAGCCAGAACTGCTGGGGTGTGCTTGTGGACTGCGTTTGTTTTATTGTCTTTTTTTTTTCCTTT
1841 GGGTGGGGGGGTGGGGAAGTGGGGAGTAAACACTAATATTGTGTCAACCTACATAATAGAAAGAGAATGTATTTTTTTTT
1921 CTTCATTTCATGGCCTCAAACATTTCTCCCATCCGTGTAAAACTTAGAAACACCCTTTGTTTCAGTTTTTCATATCATTT
2001 CCATTTTTCATGTTTTGTAAAATTTTTTTCTGTGTTCACATCAGCATTCACTTCCGTTACATTTGCCAAAGGAAGCTGTC
2081 GATATGTTTCTGTTATTATTCCTGTGTGACTTATTGTACCTCACAGTATTTATTTGTTTTGTTTTTTTTTTCCAAAATTA
2161 AGGATCATTCATTTGGGGGTTCCGGATATTTTCTGTTTTTCAGAATTTTTTAGCAGTAGACTATTAGCGATAAGCTAGGT
2241 GTTCCTAAAGCTTTATCTTTGTCTCCTTCTGAGGAAGCCATGGTTCGGCTTGCTTCTTCATTTATCCCACCTTGAGGAAA
2321 GTCCAAAAGCATTTGCAGGGCAGAAGAAGCTGTCACACACAATGTCTGTTAAATGCCAAATGTTTACGGTAAAAGCTGAG
2401 GAATTAGTAGTAACTGTTTCTGTTTCCCTGTCCCTTTGATTCTCCCAACCCTTAGCTGCTTTGCTTTGTTGTCATGTTAA
2481 ACTGAACAAACGTGTCCCTTCATTTTATAATGTACAAAACTCTGTACAGTATGTTTGTGAATAATTGAACCAGAATAATG
2561 AACATTTGTTTGCAAACATTCGGCCCTCTTAGCGTTCTCTATATTGCTGCTCTGTTGCCCTGATACCAAGTGCTGGATTC
2641 AAAGAGAATTGAAACACATTGCTGCAATCTCAGCAGGAATTGTGGAAACTGTGTTTTGGAGTGTGTGTAGACTAAGCCAC
2721 ACCTTGTTTTCTGAACACTAATAAGGATCTGTGTTTGAGCAACTTTCAAGTGTTACATTCTTAGAAATGTGAGAAATCAC
2801 CTCTACAAAAACTCAGTGTTTAGTCTGTTTAAGTGAATGCCAGTAAAATGTCAAACAGTGGTCCTGAGTGAGTCACCTAG
2881 CTGTCAGGAGGTCACATCAGCTTGCAAACCAGGCTCAGGAGAAGCATTTGTCACTTGCCACACACATGGCTAATGTCTTT
2961 CGCCAGGTCAAAAAGGTACAAGAGTAATACCACTGAAGAAGTTGTTCTTGCTAGGAAACGTGAGTGTGTGCCCGTTTGAC
3041 AGAAATGGCGCTAAAAGCCAGGCAGCTGGCACTTTCTAAGTGTACCCTATGAGCCACTACTTTTGTGCTTCACATTCCCA
3121 ATTGTTTGGGTGGCCTTTTCACAACCACGTGTTCCAATATAAAGCCTATTTGCTTAAAAAGAACGGGGAAGTAGGTGGAT
3201 GTAGATGATGAGATGGTAAACCGTGAGATGTACAGTTGTACTCCC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gaucACCAGGAUUUGUAAAGug 5'
              ||| |: |||||||||  
Target 5' ttcaTGGCCTCAAACATTTCtc 3'
 1927 - 1948 150.00 -14.70
2 
miRNA  3' gaUCACCAGGAUUUGUAAAGug 5'
            |||  |:|  | ||||||  
Target 5' tcAGTTTTTCATATCATTTCca 3'
 1982 - 2003 136.00 -7.10
3 
miRNA  3' gauCACCAGGAUUUGUAAAGUg 5'
             |||  |||   |||||:| 
Target 5' aacGTGTCCCT--TCATTTTAt 3'
 2489 - 2508 135.00 -10.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
34 mmu-miR-203-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004247 Trp63 transformation related protein 63 4 3
MIRT004268 Zfp281 zinc finger protein 281 2 1
MIRT053248 Snora62 small nucleolar RNA, H/ACA box 62 2 1
MIRT053309 Cav1 caveolin 1, caveolae protein 2 1
MIRT437354 ZNF148 zinc finger protein 148 4 1
MIRT437361 Rbm44 RNA binding motif protein 44 3 1
MIRT577405 Ung uracil DNA glycosylase 1 3
MIRT579086 Commd10 COMM domain containing 10 1 1
MIRT579797 Zfp300 zinc finger protein 300 1 1
MIRT580486 Thsd7b thrombospondin, type I, domain containing 7B 1 1
MIRT580726 Srsf12 serine/arginine-rich splicing factor 12 1 1
MIRT580745 Srek1 splicing regulatory glutamine/lysine-rich protein 1 1 1
MIRT582530 Mapk8 mitogen-activated protein kinase 8 1 1
MIRT582806 Katnal1 katanin p60 subunit A-like 1 1 1
MIRT582851 Itga4 integrin alpha 4 1 1
MIRT583438 Frmd3 FERM domain containing 3 1 4
MIRT583523 Fgf16 fibroblast growth factor 16 1 1
MIRT584165 Cpox coproporphyrinogen oxidase 1 1
MIRT584578 Bnc2 basonuclin 2 1 1
MIRT584898 Alkbh5 alkB homolog 5, RNA demethylase 1 1
MIRT585047 Ptchd4 patched domain containing 4 1 2
MIRT585735 Stxbp4 syntaxin binding protein 4 1 1
MIRT586903 Hist2h2be histone cluster 2, H2be 1 1
MIRT587655 Ces2g carboxylesterase 2G 1 1
MIRT590502 Uri1 URI1, prefoldin-like chaperone 1 1
MIRT592154 Pcdhb19 protocadherin beta 19 1 1
MIRT593932 Cyld CYLD lysine 63 deubiquitinase 1 1
MIRT595081 Zmiz1 zinc finger, MIZ-type containing 1 1 1
MIRT595094 Tlr4 toll-like receptor 4 1 1
MIRT595712 Ccdc110 coiled-coil domain containing 110 1 1
MIRT595800 Cp ceruloplasmin 1 1
MIRT600445 Rs1 retinoschisis (X-linked, juvenile) 1 (human) 1 2
MIRT604774 Prkcb protein kinase C, beta 1 1
MIRT606394 Gtpbp3 GTP binding protein 3 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-203 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-203 7,12-Dimethylbenz(α)anthracene (DMBA) NULL 6001 Quantitative real-time PCR mouse skin 24792773 2014 down-regulated
miR-203 Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-203 17beta-estradiol (E2) approved 5757 Microarray MCF-7 breast cancer cells 19528081 2009 up-regulated
miR-203 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 up-regulated
miR-203 Cadmium NULL 23973 Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated
miR-203 5-azacytidine (5-AzaC) approved 9444 Quantitative real-time PCR synovial fibroblasts 21279994 2011 up-regulated
miR-203 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-203 Curcumin NULL 969516 Quantitative real-time PCR Bladder cancer 21836020 2011 up-regulated
miR-203 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-203 Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miR-203 Estrogen NULL NULL Quantitative real-time PCR breast cancer cells. 22260523 2012 down-regulated
miR-203 Interleukin 13 (IL-13) NULL NULL Quantitative real-time PCR bronchial epithelial cells 22453679 2012 up-regulated
miR-203 Goserelin approved 47725 Microarray prostate 22674191 2012 down-regulated
miR-203 Glucocorticoid NULL NULL Quantitative real-time PCR Eosinophilic esophagitis 22815788 2012 down-regulated
miR-203 Glucocorticoid NULL NULL TaqMan low-density array Eosinophilic esophagitis 22815788 2012 down-regulated
miR-203 Tamoxifen approved 2733526 Microarray rat liver 17343880 2007 down-regulated
miR-203 Comfrey NULL 6440495 Microarray rat liver 21370286 2011 down-regulated
miR-203 Comfrey NULL 6440495 Quantitative real-time PCR rat liver 21370286 2011 down-regulated
miR-203 Furan NULL 8029 Quantitative real-time PCR liver 22079235 2011 down-regulated
miR-203 Phorbol ester TPA NULL NULL Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated
miR-203 Vitamin D3 approved 5280795 Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated

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