miRTarBase - #MIRT584588


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pre-miRNA Information
pre-miRNA mmu-mir-205   
Genomic Coordinates chr1: 193507463 - 193507530
Synonyms Mirn205, mmu-mir-205, Mir205
Description Mus musculus miR-205 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-205-5p
Sequence 7| UCCUUCAUUCCACCGGAGUCUG |28
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B4QZ0E miR-205 Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Serum Quantitative reverse transcription PCR
Gene Information
Gene Symbol Bcl6   
Synonyms Bcl5
Description B cell leukemia/lymphoma 6
Transcript NM_009744   
Expression
Putative miRNA Targets on Bcl6
3'UTR of Bcl6
(miRNA target sites are highlighted)		 
>Bcl6|NM_009744|3'UTR
   1 ATGAAGCATGGAGTGTTCCTCGCCCTTTCCTCTCCAGCCCCTTCTCAGAATCTACCCAAAGGATGCTGTAACACTTTATA
  81 CAAAGGTCATCCCATGATGTAGTGCCTCTCTCATCCACTAGTGCAAATCATAGTTGGGGTGGGGGTGGGGGTGGGGTTTG
 161 CGGGACCGGGAGCCAAGGCAGCTCCCCTTCCCACACTGCCATAAAACATTAAGAAAATACTATTGCTTCTTCTCCTATGT
 241 GTAAGGCAAACCCTGTCAGCAAAAAGCAAATTCATTTTCTATATCAAAGTAGGGGAGAATGCAGAAGTTCTGACTTGACA
 321 GGTTGCAACCGAGGAATGTAAATGATGTGTGGGAACAGAGGTCTCTTTTGTATGTAAATGTGTATTGTTTTAAAAGACAA
 401 GACTTCAGTATGCTGTCAAAGAGAAGGCTTTAATTTTTTTAACCAAAGGTGAAGGAATATATGGCAGAGTTGTAAATATA
 481 TAAATATATATATATATAATATAAATATATAAACCTAAAAAGATATATTGAAAATATAAAACTGTGTTAAAGGCTCGATT
 561 TTGTATCTGCAGGCAGACACGGATCTGAGAATCTTTATTGAGAAAGAGCACTTAAGAGACTATTTTAAGTATTGCGTCTG
 641 TATAAGTAAGAAAATATTTTGTCTAAAATGCCTCCGTGTATTTGTATTTTTTTGCAAGTGAAGGTTTACAAATTTACAAA
 721 GTGTGTATTAAAAACAAAAAGAACAAAAAAAAAAGCTGCAGAAGGAGAAATGTATACTTTTGTTCCAGTTTTCAGTTTGT
 801 ACATACCTGTAATGTGTCCTCACGGTGCCTTTTTACACGGAAGTTTTCAATGATGGACGGGTGTGCGCCATCCCTTTTTG
 881 AAGTGTAGGCAGACACAGGGACTTGAAGTTGTCACTAACTAAACTCTCTTTGGGAATGTTTGACTCCTCCCACATTCTGC
 961 GTCATGCTTGTCGTTATAATTACTCCGGAGACAGGGTTTGGCTGTGTCTAAACTGCATTAGTGCGTTGTAAAATAGAGCT
1041 GTACAAAACATAAGAATAAAACATTGAAAAGTCGA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gucUGAGGCCAC-CUUACUUCCu 5'
             |:|::  ||  |:|||||| 
Target 5' tgtATTTTTTTGCAAGTGAAGGt 3'
 683 - 705 135.00 -9.80
2 
miRNA  3' gucugaggccaccUUACUUCCu 5'
                       ::|||||| 
Target 5' ttttttaaccaaaGGTGAAGGa 3'
 435 - 456 125.00 -11.00
3 
miRNA  3' gucugaggccaccUUACUUCCu 5'
                       ||||| || 
Target 5' cacggaagttttcAATGATGGa 3'
 836 - 857 113.00 -9.09
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
24 mmu-miR-205-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003192 Pten phosphatase and tensin homolog 4 1
MIRT007192 Lrrk2 leucine-rich repeat kinase 2 3 1
MIRT013552 Dok4 docking protein 4 1 1
MIRT013553 Atp1a1 ATPase, Na+/K+ transporting, alpha 1 polypeptide 1 1
MIRT054551 Gsk3b glycogen synthase kinase 3 beta 1 1
MIRT578673 Gramd1c GRAM domain containing 1C 1 1
MIRT580923 Slc35a1 solute carrier family 35 (CMP-sialic acid transporter), member 1 1 1
MIRT581415 Rbm47 RNA binding motif protein 47 1 1
MIRT582912 Il1r1 interleukin 1 receptor, type I 1 1
MIRT584261 Clasp1 CLIP associating protein 1 1 3
MIRT584588 Bcl6 B cell leukemia/lymphoma 6 1 1
MIRT587459 Decr2 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal 1 1
MIRT588441 Yme1l1 YME1-like 1 (S. cerevisiae) 1 1
MIRT591352 Fgf5 fibroblast growth factor 5 1 2
MIRT595045 Cacnb4 calcium channel, voltage-dependent, beta 4 subunit 1 1
MIRT595313 Gpr35 G protein-coupled receptor 35 1 1
MIRT596159 Olfr287 olfactory receptor 287 1 1
MIRT596973 Tmem132b transmembrane protein 132B 1 1
MIRT597582 Ptprn2 protein tyrosine phosphatase, receptor type, N polypeptide 2 1 1
MIRT599076 Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 2 1 1
MIRT600592 Nfib nuclear factor I/B 1 1
MIRT602263 Gm4944 zinc finger protein 994 1 1
MIRT604828 Mylip myosin regulatory light chain interacting protein 1 2
MIRT604972 Gas2l3 growth arrest-specific 2 like 3 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-205 N-ethyl-N-nitrosourea NULL 12967 Quantitative real-time PCR mouse liver 21029445 2010 up-regulated
miR-205 Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-205 Benzene NULL 241 MiRNA PCR array white blood cell 24780745 2014 up-regulated
miR-205 Benzene NULL 241 MiRNA PCR array blood mononuclear cells 24780745 2014 up-regulated
miR-205 Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 down-regulated
miR-205 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-205 Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-205 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 up-regulated
miR-205 Acarbose Approved 444254 Microarray diabetic rats cells 24260283 2014 up-regulated
miR-205 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-205-5p Cisplatin 5460033 NSC119875 approved sensitive Low Prostate Cancer tissue and cell line (DU-145, PC-3)
mmu-miR-205-5p Gemcitabine 60750 NSC613327 approved sensitive Low Pancreatic Cancer tissue and cell line (MIA-PaCa-2, CAPAN-1)

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