miRTarBase - #MIRT584883


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pre-miRNA Information
pre-miRNA mmu-mir-351   
Genomic Coordinates chrX: 53053255 - 53053353
Synonyms Mirn351, mmu-mir-351, Mir351
Description Mus musculus miR-351 stem-loop
Comment The predominant miRNA cloned by Langraf et al. has a 3' terminal U residue, which is incompatible with the genome sequence .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-351-5p
Sequence 16| UCCCUGAGGAGCCCUUUGAGCCUG |39
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Antxr2   
Synonyms 2310046B19Rik, AW561899, CMG-2, CMG2, cI-35
Description anthrax toxin receptor 2
Transcript NM_133738   
Expression
Putative miRNA Targets on Antxr2
3'UTR of Antxr2
(miRNA target sites are highlighted)		 
>Antxr2|NM_133738|3'UTR
   1 GACGGGAGAACAAAGAATGAGAAGATAAGAAGACAGTGTGACAGTGTATCTTCATGATGCTGATTTCCAACAGAACCGAC
  81 AGTCCGGTGCATCTCAGAAGTTCTTGGGACAACAGCCCATTTTCTCTCTGTCAGGAAATGTTTCCTCTGCCTTCTGCTTT
 161 CTGTGCACTAAACATTTTCCAACACTTGTTCTGCCATCGACATGAGAGGTGATGAAAGTCATCGGCGATAGCCCATGATT
 241 CACGACACTGAAAATCCCGAGGAATGTTAGTTTGCATGCTAGGGTTTATGCAAAGCTCGTTTTGACTATGTAAGAGGACA
 321 AAGCAGACACATCGATGATGTAATGATACCAAAGCTAGGACTGCAAATCCACCAGCCACAGAGGTTTGCAATGGAGACTG
 401 GTGATTCTGCCATGAATGTGTGGCCCCTGTGCTTTTGTTTGGCAAGATCTTTAGCTACAAGCAAAACATGAAGTTTCCTC
 481 CCAGGCTAAACAGATAATGGAGTCCACTGCCTTGTAGCTATGTCAGATAGCAAAGCCTTTCCAAGTCCTCCATTACTTTG
 561 TGCCTTACAGGAAATTTCTGACTAGAAAATCTTGTCACTGTTACACTGAAAGTGCACACGCATGACAAAATGTAGACGAG
 641 ACGCCTCAAGGTACTGGATGCAAGCAGGATTTTTGCCCTTTAGTTTTCCAAGACACCTTTCTTTTATTATGCACTTGAGA
 721 CAAGAGAATTAATAGAGCGTTAATTCAACAGGAAGACCGCCTCCAACCAAAGACCTGGAGCGCAGCATAAGGACTTGTGA
 801 TTTGAGACGTTGTCCCCAGCCTGGTAGATCCCCCTTTCTCAGCATTTGGGATTTAGCAGTGCATAAAGCATTAATATCTG
 881 TAAAAACACCTAGATGTTTGTTTGGCTTTTAATTTAAGGAAGCTGCAACCACAAAGCTTCCGCTCAGGGTTTTTTCTTCC
 961 TTCAAGTCTCCAAGGGCTCTTCAGCGTCACAAGCCAGCAACTCTCTTTGCATGAAAATTTCAAAGTTTAATTAATATAAT
1041 TAAAGGCAACAGCAAGCAGCAGCCTGTGAAGATTTTGCTCATCTTTTTTATGCCTTTTGACATTGAGTGACCTATCACTG
1121 TATGCATGTTACTTAGAAATTGAGGAGCACCACACCTTGGTTGTGGTTCAGCCTGGGAAAGAGACCTCCTTCCTTCTGTT
1201 TATAAATTAAAATCAGGAGGGGCGCCATCAGAAAGCATGGACAATATACATACTATAAATTTTTAGAAATATCACCATCG
1281 TGTCACGTCAACGATGCCAAATTATGTTAGTGTGAGCAGAAACCCGGTGGGGGAGGAAGGCGGCAGCAGCCGAAGGAAAT
1361 AGCTCAGATAATCTAGTCACTTTCGATACTGTACTTCAGATGCGAAATGGATATTCGACTGGAAACCTGACAAAGCGCGC
1441 CTGCTTTGATGTGAACTGTTATAGACAATGACCAGTGGCCGGGTCAGTGGGATGTCTCTCTGCGAGCACAAAGGCTTATC
1521 AAATGACACTAAAAATAAGTTCAACAACCATCACTTTGGAAGGGAGAAGGCGAACATTTCATGTTTGGCGGGCATGTGAG
1601 TGCAGGAGATGGAAAGAGCCATTTAGAGCATCCTCATATAATTGCCCGCATTGTACTCTTCATGGAAATTTCAAAGGACG
1681 GCAGTGATATTTTTCATTGGTGTCCACGTTTGTGGCACTGCTCCAAGAAGCCTTATGCACACATACAAATATACAAATGC
1761 ACACACCTACACTCTCTAGCTTTAATCTTTTTGCTCAACCTTATTTATATCACTGACTGGCTGGATCCAAAGTCACACCT
1841 CCACATATTAGTGAATAAAAAATTTTTACCTGTGAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guccGAGUUUCCCGAG--GAGUCCCu 5'
              | |||| | :||  ||||||| 
Target 5' caacCACAAA-GCTTCCGCTCAGGGt 3'
 926 - 950 157.00 -17.30
2 
miRNA  3' guCCGAGUUUCCCGAGGAGUCCCu 5'
            | ||| ||||||||:|||| | 
Target 5' aaGTCTCCAAGGGCTCTTCAGCGt 3'
 964 - 987 146.00 -27.30
3 
miRNA  3' guccgAGUUUC-CCGAGGAGUCCCu 5'
               | :| | | | ||||| || 
Target 5' aaatgTAGACGAGACGCCTCAAGGt 3'
 628 - 652 119.00 -11.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
22 mmu-miR-351-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT005493 Tnf tumor necrosis factor 3 1
MIRT005494 Siah2 seven in absentia 2 3 1
MIRT054029 E2f3 E2F transcription factor 3 4 1
MIRT580229 Tspan12 tetraspanin 12 1 1
MIRT580251 Trim71 tripartite motif-containing 71 1 1
MIRT582369 Mtf1 metal response element binding transcription factor 1 1 1
MIRT582453 Mgat4a mannoside acetylglucosaminyltransferase 4, isoenzyme A 1 1
MIRT584883 Antxr2 anthrax toxin receptor 2 1 1
MIRT587194 Foxk1 forkhead box K1 1 1
MIRT588849 Snrnp40 small nuclear ribonucleoprotein 40 (U5) 1 1
MIRT591277 Lars2 leucyl-tRNA synthetase, mitochondrial 1 2
MIRT592913 Madd MAP-kinase activating death domain 1 1
MIRT594892 Il1rn interleukin 1 receptor antagonist 1 2
MIRT596428 Hif1an hypoxia-inducible factor 1, alpha subunit inhibitor 1 1
MIRT599144 Dqx1 DEAQ RNA-dependent ATPase 1 1
MIRT599715 Aph1c aph1 homolog C, gamma secretase subunit 1 1
MIRT600831 Gm14137 predicted gene 14137 1 1
MIRT604900 Jmy junction-mediating and regulatory protein 1 1
MIRT605442 Stat1 signal transducer and activator of transcription 1 1 1
MIRT606093 Wdr25 WD repeat domain 25 1 1
MIRT732643 F8 coagulation factor VIII 3 0
MIRT734828 Sox11 SRY (sex determining region Y)-box 11 2 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-351 Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 up-regulated
miR-351 Longevinex NULL NULL Quantitative real-time PCR ischemia/reperfusion [I/R] rat model 21203465 2011 up-regulated
miR-351 Longevinex NULL NULL Quantitative real-time PCR normal heart 21203465 2011 up-regulated
miR-351 Resveratrol NULL 445154 Quantitative real-time PCR ischemia/reperfusion [I/R] rat model 21203465 2011 up-regulated
miR-351 Resveratrol NULL 445154 Quantitative real-time PCR normal heart 21203465 2011 up-regulated
miR-351 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 up-regulated

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