miRTarBase - #MIRT585047


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pre-miRNA Information
pre-miRNA mmu-mir-203   
Genomic Coordinates chr12: 112130880 - 112130955
Synonyms Mirn203, mmu-mir-203, Mir203
Description Mus musculus miR-203 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-203-3p
Sequence 48| GUGAAAUGUUUAGGACCACUAG |69
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Ptchd4   
Synonyms 3110082D06Rik, Gm321
Description patched domain containing 4
Transcript NM_028474   
Expression
Putative miRNA Targets on Ptchd4
3'UTR of Ptchd4
(miRNA target sites are highlighted)		 
>Ptchd4|NM_028474|3'UTR
   1 GGGTGGAGACTTTTTCTTTTCCAGTATTGCACAATGATGCAGGGCTAGTAAGGCTCAGACCTCAGCTGCTTGGGCTGGCC
  81 AGGGGTAAAAAGGCAGGTCAGATCAAGAGTGCATTTTTCATGACGCATCCAAGGGTCTGCTTCTTTTGATGGAAGAGATA
 161 ATAAAAGGAGGTGAAGTTCTTTACAACCTTGTGCTCCATGAGAGATAATTCTCCAGACATTGATCTTAGAGCTTTTCTGA
 241 GAAATGGATGGATTGGTGTGGTCAGATGGAAGGCCGAAAGATCTAAAGATTCTTAGAAGAAATGGAGAAGAGAGAATAGG
 321 TGGTCTCCAGATAGGGGAAAGTACAAAAAGAAATGTCTAATTTGTCATGTCACTTCATGTTTTCATGAACAGATCAATCA
 401 AAGGAAACCTATGCACATGGGAAGTACACATTTAATCCTGTATAAACCAAAGGACCGGACCTCCCAATCCACTTTGTGTG
 481 TACATATAAGTATACATATACATAATATGTCTTGAGTGTGTGTTGGTGGTTGGATGAAAGAAGATGTTCATTTGCAGATA
 561 GGCTGGCCTTTCTCAAAAAGTCAAATCAACATTAGTCAAATATATAACCTCATTAGGGATGATACAAAATATATAATTGG
 641 TCACTTGAAATCAACTCAACTGTCTAAATAGCAAGAGAATTATCAACTGTGTGATAACAAGATAGACAGAAATTTGAATC
 721 CATTAAATTCTACCTATCATGTTGTTGGTCACTGAGATCCTACGTTTGGGATGACTTCACCAAAACTCCAAAAGGTATTT
 801 GAGTTAATTAGCATGTGAACAAACGTTTAGAATCTAAAGTAGTTGAGATAGCTTGTCATTAGCTTTTAACATTTCTGTGT
 881 ATTTGGAAGGCCAGCATGGCTACTTTTGGATATCTCATCCACCTGAAAAAAATGAGCCCTTTAAGCACATTGTTTTTGCA
 961 TGCATATGTAAGGGTACACAGTGTAAGTGAGAGATGGAAAGAAATGATGAGATACCATTTCGTAGTTAAGAGCTCTTAAG
1041 TTAAATGGTGAAAATACACTGTTGTGGCACAGGTGGCAATTAACACAGGAAAAAGAAAATGTCTGGAAAATGTAGCGAGT
1121 AAAATGAGCCATCTACCATTCACCATCTCGGCATCAAACAGCCTGTTGTTTAAGAAAAACTTGCCAACTCTACAGACTCT
1201 AATGTGTTCCTTGGAATAAGTCTGGGAGCATTAACCAAGACCATGATCACTATGATGGTGGTCACCATAACCCCTGCAGC
1281 ATCATTTTGTAGCTTCTTGGAGTATCATTGTCTTCATTAACTAGAAAGTAATCTCCTATGGCAAGCACAGCTCTCTACTG
1361 GTGCCATATTAACTGTGTGTAATTGCACCTGGAGTCCTTACATTAAAAAAAGAAAGAAAGAAAGAAAAAGTATTGAGGTA
1441 AATGTTTTGGGTTTTTTTGGTTTTGTTTGTTTGTTCGTTTGTTTGTTTGAGTTAGTGTTGAAAACTATGCCCCAGGCAAG
1521 TACTCAGTCTGCTGGGTGCGTTCAAGGACTAAGGCTGCTATTCATGTTGGAGAACCTTACTAGAAAATTCCTTTTCCATG
1601 CCAAATAAGGAACCTAAGGGATGATTCATTTCTGGGTAAAATAAAACAAATGCCTAATGATTACACATCCTGCTAAGAAG
1681 CTCTTTTTCCTGAGGAACATTTACATTTCCTACCAATTTGGGTTGTAGAATTTAAATGCATTAACAATACTATTGGCTTT
1761 AAAAAACAAAACACAACTAAAACTCTTAGAAAATTCCATTCCTTTTAAGTTTTAGGAAATAATGCTGATAGACAGTACTG
1841 CTCTTCTAAATGATGCAAATTTTTAAATGGACATCATTTAAATGAAACTTCAGTTTATTGAATGACATTTGTTCTATTCT
1921 ACTTTAAAACTGCTGTGTTTCAATGTCTCCCCTGATGTGGATACAATAGCTATAGAATTAAGGAGGGACCATAACTTCCC
2001 ATAACCGCACTTGATATTTTCACTGTATCTGTAAAGCCATGTCACAGGTAACGCCAGCCTTTCATTGCAAGAACTTTTGT
2081 CCTAGGGGGAACAGACATTTGGACAGTTGACCTCCAGGCCATGATCTCATATTTGAAGTCGAATTTTCTAAGCAGTTTTT
2161 GCAGTTGGAGAACAGAATTCCTCTTTCAAAGTTACTCATCGTTTATGAAAATTTCTATCACTCCCACCTGCTTGTAAAGA
2241 CAGCCACTAAGAAGCTGAGGATTCCATATCACTTCATATCTGTCCATATTCAGTGAAACTCCATTGTTTCACCAATTGTT
2321 TATTTAAAGATAACTTACTTGTGTTTTATAATTGCCATACCATTTCAGATACAAAAAAAAAAAAAAAAAAAACCCAACAA
2401 CAACAAAAAGATTGACAAGCTAATTGGCAAGCTTGCAAACTGCCAAATACATGGAAGTACCGCTGCATATTATGCCAAAG
2481 CACCAGTTTCAGTCTCTCTCAAAGCACTTGGATGGGGAAAGACTTGTTTATATATTAAGTATTGTCTTCAAGGATCAGAC
2561 GTGTAGGACAAACAAGAATTTCTCATTGTCCAAGTGTCTAGAGAGGTGGCATTTTAGAAGAGATGTTAACTTCTCCAGAA
2641 CACGTGATTCTCTCCAAGGATATGTAGCAATAATTTACTGAAATGAAACTCCGTGTTCTGGAAGAGTTAATAGGGTGCCC
2721 AAAATTCCTTGTCTTGGTCTTAGGAAGTAATGTAAGCCCAGAAGAACCTCGCAAAGCATCCGGCAAACCTTCCAACCTCA
2801 CACTGAAGCAGATATTTCCAAGATTTCCTTCACTGAGTACCAAGTATTAAAATATTAGATCATGATTTGCAGGTAAACCT
2881 CCAAACTCAACCTTTTCTGATTATTGGTATGCTCCATCCTAGATTTTCAAGTTTTTGCTTGCTTTATCCATTGTGAAGTG
2961 CTGATGGCATTCAAGTTAGGACGTTATCGTCTATCAAACAGAGGGGCCTTTCAAACCCTAGAAGAAAACAGTGGTGAATC
3041 TTTTAGAACTGTGGAGTATTTTATATACTGTAGTGTGTTTTCCCCTTGATAGAAGAGATTTTTAAAAACTTGTGAACCCC
3121 ATACTTTTTCTTGTTTAAATTGGACTGTGTTCCCCCCCCCCACAGAATGTGAAACAGAAAATATCATTTTATGTCCCAAT
3201 CTCCCCAAGGAGGAACTCACCTAAATTATCAGTCAAGATTTGTCTAAAGGTTTCGTGGGCTGACCTACATCATGTGGAAG
3281 TCTGTTGAAAGAATATATAGCTACATGTTTTCTGGTGGGTTCTTGCAGTGACCATGAACCTCTTTGAAGGCTGAGCATGA
3361 GTACTCTATGACAGAGCTATTCTCAACTAAGTTTGTATCTCATAAATGTCTTTCTTCCAATTTATTTCCAATTGTTTTCC
3441 ACAGAAATGAACTTACATGTTTTATTTTGTATTGTGAAAGAAAGAAGTGTGATACCCGTAGCTTCTACGATGCTATAATA
3521 GCCATTCTTGTATGTTGATCTTGAGTGTAAGAGCTATGTCCATTCTGTAATTATCCATTGCACTTTACCATAACAAGCTA
3601 ATCCTTCTAGGCTTGCGATTCGAGCTGGGGATTAATTATTGCCATTCAGAATTGTGTGTATTTTTATATGTTGACTGTAA
3681 ATATTGTTATTACAGTCAAATATTTTTATGACCAAATATTTATAAATGCATGGCACAATGCACGCATGATTACTAGTATG
3761 TAGATGATTAAAGACACTATTTTCAGGGTTATTACACTTTTTATTAAAATTCTCATATTCTCTATC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gaUCACCAGGAUUUGUAAAGUg 5'
            | ||  ||  | ||||||| 
Target 5' taATTG--CCATACCATTTCAg 3'
 2349 - 2368 148.00 -6.80
2 
miRNA  3' gaUCACCAGGAUUUGUAAAGug 5'
            | | | ::| ||||||||  
Target 5' tcATTAGCTTTTAACATTTCtg 3'
 856 - 877 140.00 -7.30
3 
miRNA  3' gaUCACCAGGAUUU-------GUAAAGUg 5'
            ||||  :|||:|       |||||:| 
Target 5' caAGTG--TCTAGAGAGGTGGCATTTTAg 3'
 2591 - 2617 136.00 -8.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0000236
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013581. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast HITS-CLIP data was present in GSM1385343. RNA binding protein: 聽AGO2. Condition:C2C12_DM_Ago2_CLIP-Seq_myotubes ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
34 mmu-miR-203-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004247 Trp63 transformation related protein 63 4 3
MIRT004268 Zfp281 zinc finger protein 281 2 1
MIRT053248 Snora62 small nucleolar RNA, H/ACA box 62 2 1
MIRT053309 Cav1 caveolin 1, caveolae protein 2 1
MIRT437354 ZNF148 zinc finger protein 148 4 1
MIRT437361 Rbm44 RNA binding motif protein 44 3 1
MIRT577405 Ung uracil DNA glycosylase 1 3
MIRT579086 Commd10 COMM domain containing 10 1 1
MIRT579797 Zfp300 zinc finger protein 300 1 1
MIRT580486 Thsd7b thrombospondin, type I, domain containing 7B 1 1
MIRT580726 Srsf12 serine/arginine-rich splicing factor 12 1 1
MIRT580745 Srek1 splicing regulatory glutamine/lysine-rich protein 1 1 1
MIRT582530 Mapk8 mitogen-activated protein kinase 8 1 1
MIRT582806 Katnal1 katanin p60 subunit A-like 1 1 1
MIRT582851 Itga4 integrin alpha 4 1 1
MIRT583438 Frmd3 FERM domain containing 3 1 4
MIRT583523 Fgf16 fibroblast growth factor 16 1 1
MIRT584165 Cpox coproporphyrinogen oxidase 1 1
MIRT584578 Bnc2 basonuclin 2 1 1
MIRT584898 Alkbh5 alkB homolog 5, RNA demethylase 1 1
MIRT585047 Ptchd4 patched domain containing 4 1 2
MIRT585735 Stxbp4 syntaxin binding protein 4 1 1
MIRT586903 Hist2h2be histone cluster 2, H2be 1 1
MIRT587655 Ces2g carboxylesterase 2G 1 1
MIRT590502 Uri1 URI1, prefoldin-like chaperone 1 1
MIRT592154 Pcdhb19 protocadherin beta 19 1 1
MIRT593932 Cyld CYLD lysine 63 deubiquitinase 1 1
MIRT595081 Zmiz1 zinc finger, MIZ-type containing 1 1 1
MIRT595094 Tlr4 toll-like receptor 4 1 1
MIRT595712 Ccdc110 coiled-coil domain containing 110 1 1
MIRT595800 Cp ceruloplasmin 1 1
MIRT600445 Rs1 retinoschisis (X-linked, juvenile) 1 (human) 1 2
MIRT604774 Prkcb protein kinase C, beta 1 1
MIRT606394 Gtpbp3 GTP binding protein 3 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-203 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-203 7,12-Dimethylbenz(α)anthracene (DMBA) NULL 6001 Quantitative real-time PCR mouse skin 24792773 2014 down-regulated
miR-203 Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-203 17beta-estradiol (E2) approved 5757 Microarray MCF-7 breast cancer cells 19528081 2009 up-regulated
miR-203 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 up-regulated
miR-203 Cadmium NULL 23973 Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated
miR-203 5-azacytidine (5-AzaC) approved 9444 Quantitative real-time PCR synovial fibroblasts 21279994 2011 up-regulated
miR-203 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-203 Curcumin NULL 969516 Quantitative real-time PCR Bladder cancer 21836020 2011 up-regulated
miR-203 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-203 Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miR-203 Estrogen NULL NULL Quantitative real-time PCR breast cancer cells. 22260523 2012 down-regulated
miR-203 Interleukin 13 (IL-13) NULL NULL Quantitative real-time PCR bronchial epithelial cells 22453679 2012 up-regulated
miR-203 Goserelin approved 47725 Microarray prostate 22674191 2012 down-regulated
miR-203 Glucocorticoid NULL NULL Quantitative real-time PCR Eosinophilic esophagitis 22815788 2012 down-regulated
miR-203 Glucocorticoid NULL NULL TaqMan low-density array Eosinophilic esophagitis 22815788 2012 down-regulated
miR-203 Tamoxifen approved 2733526 Microarray rat liver 17343880 2007 down-regulated
miR-203 Comfrey NULL 6440495 Microarray rat liver 21370286 2011 down-regulated
miR-203 Comfrey NULL 6440495 Quantitative real-time PCR rat liver 21370286 2011 down-regulated
miR-203 Furan NULL 8029 Quantitative real-time PCR liver 22079235 2011 down-regulated
miR-203 Phorbol ester TPA NULL NULL Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated
miR-203 Vitamin D3 approved 5280795 Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated

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