>Wfs1|NM_011716|3'UTR
   1 GGAGCGTCCGCCGCTGGAGGAGGCTTTGGTGCATGTTGCTGTGAAGTCCTTCCGTGTGGCCACCCAGCCAGCTGGAGCAG
  81 CACTGTGCCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTATTCTGCTCTCGTGTGGTTAGATCCCAGG
 161 CTCTCTGCTCACCTGTAGATCGCAGATCCTGCTGGAGGGTGGTTCTCTTTAGCACTGTCCACTTTGAATGCCGAGTGTCA
 241 TAAGAAATTGCATGCTATCTTCACTCACAATCCTGCCCTTCCCTCACAGAGCTGGAACTCCAAGCCTGGCCCCAAAGACC
 321 CTCCATCACGAAGAGCACTTTACAGATAAGAATCTTCCTCCCCAGTTCTTCATGCCTCCTTCCTGCCCTTTCCTTACTTT
 401 GTGTTGGATTTGTTTTCCAAATATGTGTGTATGTAGACTTCATGGTAGTGTTTCTTATTTATTTGGTTGCTGCTAAGCCT
 481 TGACAGTGGTTCACCTTCCTGGGCTGTCCCCAGTGGTCACGTCCTGCCTGGCTTCCTACTTGGGTATAGCATGTCCAAAC
 561 TGGGCTCTGAACACTACAGCCTGCCTTGGAGCTGGCCTATCTCTGGGGGTGTTAGAGAGTTTGTGGGGATCTCTTCTGAG
 641 TACCCCAGGACTTGGGAAATAATGCAAGAGTATCCCTTTCAGTACATAGGTAAGCTTGGCTATGTTCAATATGGCAGAGC
 721 CAAAAGCCAAGTTCTAAGGCAGCCGAAATAAAAAGCTTTGAAACCTATA

- BMC genomics, 2013

BACKGROUND: Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs. RESULTS: miRNA recruitment to the RISC is highly dynamic, changing more than five-fold for several miRNAs. miRNA recruitment to the RISC did not correlate with changes in overall miRNA expression for these dynamically recruited miRNAs, emphasizing the necessity to determine miRNA recruitment to the RISC in order to fully assess the impact of miRNA regulation. We incorporated RNA-seq quantification of total mRNA to identify expression-weighted Ago footprints, and developed a microRNA regulatory element (MRE) prediction algorithm that represents a greater than 20-fold refinement over computational methods alone. These high confidence MREs were used to generate candidate 'competing endogenous RNA' (ceRNA) networks. CONCLUSION: HITS-CLIP analysis provide novel insights into global miRNA:mRNA relationships in the regenerating liver.

- Cell, 2014

MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.