miRTarBase - #MIRT585915


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pre-miRNA Information
pre-miRNA mmu-mir-664   
Genomic Coordinates chr1: 185242975 - 185243043
Synonyms mmu-mir-664, Mir664
Description Mus musculus miR-664 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-664-3p
Sequence 38| UAUUCAUUUACUCCCCAGCCUA |59
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Slc16a9   
Synonyms 1200003C15Rik, 4930425B13Rik, AI552636
Description solute carrier family 16 (monocarboxylic acid transporters), member 9
Transcript NM_025807   
Expression
Putative miRNA Targets on Slc16a9
3'UTR of Slc16a9
(miRNA target sites are highlighted)		 
>Slc16a9|NM_025807|3'UTR
   1 AGGGATATTAAAAGGCCTTATTTTCTCTATTTTTCTACTTTATACCATACAGCAATATGTGTATGTATGTATGTATGTAT
  81 GTATGAATGAATGAATATTCGTATATAACAAATTGTTAGGCAGGTTCTAGAGTCACAATCTTATAGCAAAACTTCTCTGG
 161 CTGACTCTGAAGGACAACGCACGCTCTGTCTTTGTGTGCCTTGTATAACCTTGATTCCTTGTCTTCCCCTCCCCCACCCC
 241 TCGAGAAGTGGGACTGTTCTGTTCTGTTCTGTTTCATGGTTGTTCGTTGTTCATGAGTTCAGAATTAGCCTCGCACAGTT
 321 TTCTCTGGATGAAATTTAAAGTCTCTGCTTTCCCCGTGAGTTCTCTAAGGTGCTCAGTGGGTCCTGTCTTAAACTGTGCG
 401 CTCCTCACAATTGGATTTGCGTGAAGGAGTAATGGCTGTCTCCTCGATGTCAGACCTGCAAATCCAGAGAAGACAATGAG
 481 CCATTTCATTTCCGGAAATGAAAGCGGTTGCAACATCGCTGAGCTGTCGAAGACCTTAGACAAGATAGTTTTAAATCACA
 561 GATGTCTGACGTCCGTTTTGTTTTACTGTGCTCGATGAAGTACTGTGAGGCAGTTTGGTGTCCACTGCCTTCGAACTCAC
 641 TCGATTCCTCCAGCAGTCCGCCTGCATGGCCGGCTCCTAGGACCTTGCTCGCTGGTGTTGATCGGCAACCACTCTGCTGA
 721 TGTCTCCAAATAACCTGTTGGTTTGCCGGTTTCCGCCTTGGAAATAGACCTTTGAACCATTCCGAAATCTGGCCAATGGG
 801 AAGCGCCCATTTGCTTTGAGCACCTCGAGAACGGGTTGCAAGGCCTTGCTTGCATGTCTTCCAAATGAAGTCCGTGGTGG
 881 TTTCTCTTGGGCTTTTCCACTATTGCGTTTCGATTTGTGGACAGTGCTAAAAAAAAAAAAAATGATGCTTTCGGTCCATA
 961 AGATGTGGGAAAGGACAAGGTAAAGAGAACTCGCTACTCTCACCCAACCGTATTTAATCCCAAGAACAATGTTTGAGGGT
1041 GAAAAATAATGTTTAATAAATGGGCTTTTTTTTCCTCTATAGAAATGGGTTTCAAGTTTGGTATGGCTGGAAATGCCCTG
1121 TGGAAACTTAAATGCGTGCTACTATGCTGCTTTCCTTTGCATTATTTTTTGAGACAGGGTTTCTTCTGCGTAGCCTTAGC
1201 TGATCTGAACTTGCAGTATAGACCAGCCTGGCCTTGAACTCAGATCCACCTGCGGCTGCATCCCAAGTACTGGGATTAAG
1281 TAGGAGTACCTTTCCTGGCTAGTACTGTTTTTCTTGAATAAAAGGAAACAAACAAACAAACAAACAAACAAGATAAACCA
1361 TGAAGTCAGGTATTATAAAACAAACAGCCAAGGGTTCTTTAAGTAAGGGCTCCCTTTCTGTTGTGCCAAAATTTTCCTTT
1441 TTTTTTTTTTTTAATATGTGAAATGATTTTGCAGGACTACATTGCCTTCCTTTGGGGACACCTGAAGCACTTTATTACAC
1521 ATCCCGGATAGCACCTCCCAGAGGTACAACTGTGTCCTGTGTCATCAAGTAGAAAGATTCCATCGCATAGTCAGTCCTGT
1601 GGCAATGCAATAATGGGGCTCCAAGCACATGAGACTTGTGTTCTACACTCAGACTTCTGGAAAGTTCTTTGTACCTCATT
1681 CTTCAGTCTATCAAAGTTAGTAACTAAAGCCTAACTCCAGCTCCAGGGGATCCGGCACCCTCTTCTGTCCCCCACAGCCA
1761 CCTGTGTATTGGTGGAATATATATATATATAATATATATATATATATATACCCCCACACATAAATAAAAATAAAAAAAGA
1841 TGTCTTTAAAATTTAGAGCTATGATTTTTTTTAAGCCAAATAAATAAATAAAAATT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auccgacccCUCAUUUACUUAu 5'
                   || |:||||||| 
Target 5' tgtatgtatGAATGAATGAATa 3'
 76 - 97 153.00 -7.90
2 
miRNA  3' auccgaccccuCAUUUACUUau 5'
                     | |||||||  
Target 5' atttcatttccGGAAATGAAag 3'
 483 - 504 127.00 -5.40
3 
miRNA  3' auccGACCCCUCAUUUAC-UUAu 5'
              ||| ||    |||| ||| 
Target 5' agtcCTGTGG---CAATGCAATa 3'
 1593 - 1612 115.00 -5.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' auccgaccccucAUUUACUUAu 5'
                      |:||||||| 
Target 5' uguauguauguaUGAAUGAAUg 3'
 		18 - 39
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
34 mmu-miR-664-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577936 Prl5a1 prolactin family 5, subfamily a, member 1 1 1
MIRT578554 Hopx HOP homeobox 1 1
MIRT579006 Cyp19a1 cytochrome P450, family 19, subfamily a, polypeptide 1 1 1
MIRT579669 1200011I18Rik GPALPP motifs containing 1 1 1
MIRT580267 Trim12c tripartite motif-containing 12C 1 1
MIRT580632 Sycp2 synaptonemal complex protein 2 1 1
MIRT580981 Slc12a2 solute carrier family 12, member 2 1 1
MIRT581678 Ppp1r3d protein phosphatase 1, regulatory subunit 3D 1 1
MIRT582092 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 1
MIRT582126 Nr2c1 nuclear receptor subfamily 2, group C, member 1 1 1
MIRT583126 Heca hdc homolog, cell cycle regulator 1 1
MIRT583594 Fam78b family with sequence similarity 78, member B 1 1
MIRT583662 Fam160b2 family with sequence similarity 160, member B2 1 5
MIRT584399 Cdc27 cell division cycle 27 1 1
MIRT584815 Arid4b AT rich interactive domain 4B (RBP1-like) 1 1
MIRT585915 Slc16a9 solute carrier family 16 (monocarboxylic acid transporters), member 9 1 1
MIRT586057 Rnmt RNA (guanine-7-) methyltransferase 1 1
MIRT586080 Rgs4 regulator of G-protein signaling 4 1 1
MIRT587942 Atl2 atlastin GTPase 2 1 2
MIRT590595 Atp2a2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT590735 Ado 2-aminoethanethiol (cysteamine) dioxygenase 1 1
MIRT591492 Aqp3 aquaporin 3 1 2
MIRT594084 Scoc short coiled-coil protein 1 1
MIRT594733 Zfp62 zinc finger protein 62 1 1
MIRT595057 Aldh1l2 aldehyde dehydrogenase 1 family, member L2 1 1
MIRT595113 Rgs17 regulator of G-protein signaling 17 1 1
MIRT595239 Rhbdl1 rhomboid, veinlet-like 1 (Drosophila) 1 1
MIRT595762 Otud6b OTU domain containing 6B 1 1
MIRT595778 Mc1r melanocortin 1 receptor 1 1
MIRT596728 Wdr59 WD repeat domain 59 1 1
MIRT598239 Mlph melanophilin 1 1
MIRT600005 4930563E22Rik RIKEN cDNA 4930563E22 gene 1 1
MIRT602439 Emx2 empty spiracles homeobox 2 1 1
MIRT736865 Smad4 SMAD family member 4 4 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-664 Acarbose Approved 444254 Microarray diabetic rats cells 24260283 2014 up-regulated
miR-664 Sorafenib approved 216239 Quantitative real-time PCR hepatocellular carcinoma 21530512 2011 up-regulated
miR-664 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated

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