miRTarBase - #MIRT586660


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pre-miRNA Information
pre-miRNA mmu-mir-7a-1   
Genomic Coordinates chr13: 58392779 - 58392886
Description Mus musculus miR-7a-1 stem-loop
Comment miR-7a (previously named miR-7) was predicted by computational methods using conservation between mouse, human and Fugu rubripes sequences . The ends of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure
pre-miRNA mmu-mir-7a-2   
Genomic Coordinates chr7: 78888277 - 78888373
Description Mus musculus miR-7a-2 stem-loop
Comment miR-7a (previously named miR-7) was predicted by computational methods using conservation between mouse, human and Fugu rubripes sequences . The ends of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-7a-5p
Sequence 19| UGGAAGACUAGUGAUUUUGUUGU |41
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Mbp   
Synonyms C76307, Hmbpr, R75289, golli-mbp, jve, mld, shi
Description myelin basic protein
Transcript NM_001025245   
Other Transcripts NM_001025251 , NM_001025254 , NM_001025255 , NM_001025256 , NM_001025258 , NM_001025259 , NM_010777   
Expression
Putative miRNA Targets on Mbp
3'UTR of Mbp
(miRNA target sites are highlighted)		 
>Mbp|NM_001025245|3'UTR
   1 AGAAGCTGTGGGTTTAAATGCGGAAAGGAAGGAGAGGTGGGCAGGTGGAACTGGGCCAGTCACCATCGCAGAGCAACCGT
  81 GTCCCTGTGTCTGAGATACTAGCCCTGATCGGACAGTGCTGATGGCCTGGGGGATTGGGCGTGGAGTGGGCGTGGTCGCC
 161 ACTTAGCCAATTTCTACTTGTTTTGCTCCCTTTGTCCTTCTCACACGGGACTGCGATTGGGTGTCACTCAGGAATGCAAT
 241 GCCTTCTGCATCCTAACTTGAGGCACCCAGGATGTAGCATTGAGCCTATGGGTTCTTAGGTATACATGCCCTATGCCTGG
 321 GGCTTCAGCAGATCCTGCCTCTGCGCAGTGCTTCCGGGTTTCGAACCTCAGCTGTGCTCATTCTCTGCTGGGTACAGGCT
 401 GCTAATTGCTTGCCGCTGTGAAACAATGGTCCAATTGAGGCGAGGAGGTCTGCCGACTGACCTCTGCACCTCCAGCAAAC
 481 TTTCCTCTTTGTCCTATGTAGTTTGGTGGGGGTGAATGAATGAATGGTGTTCATGACAAAAAAAAAATGTGTGATTCCGT
 561 TAATTTAAATATAACTTGTGTTTCATGATGTTTCAAAAGCAAATGCCCTGTGTTAAAATGTATCAGAAGATAAAATTGTG
 641 CAGATGGTCTAGTTTCCAAGGGCAACATCTGGGGTGGGTTACTCATGAGCCCGTGGTTCAGGGTTTGTCTGTCAGGTGGC
 721 TGCCACCTTGTAAGTGTATGGCATTTTTCCATCTCTCTGTTGGATAGCTGAGCAACTCTTGGGAGAGATATGGATGGGCC
 801 ATTAAAACAAGCCCAGGTCTTTGAGTACCCTCTCAGCCTGGGCAACTTTTCTGCCTGTAAGTTGTGTCTGTTGTCAAAAC
 881 TCTGGCGAACGCAAGAGGCTTTTGACTGAGATGTAGGGCAGAGGCCCCTCCACGGCTCTAGGGCCATTGGCAGTGCAGTT
 961 TTGAAAATGCTGTAGTCTCCAGCCACACCTTTGAGCGTGGGGATGGTTGTGAAATGCTGTACCTAATGACGGCTCTCCTG
1041 CCCCGTGGCCACCAGAGCCCCTTGGTGTCTCAGCAGCAGTCTGGCCCTCGCCTTGGATGTCAGCGCTACTTGTCATTAAC
1121 ACTGGATATGTCTCACTTGCTAACAGAATTCAAGCTGCCCCAGATTCCTGCAGTCTGTACTTAGCGGTATCGCCTTCAGC
1201 CTGATCCATCAATCTTGTTTCCAGGGCCGATTTACATTGTAGTGGCTTCCAAGCACTCATCTCGAGGGAGGCGCTTGAGA
1281 TCTGGAGGATCCTGACTGCCCCCTGAGGTCCATCTGTGTAGCCAAGCCCACACATGCACACAGCCGCACCCAGTCCAGGC
1361 AGCCGAAGGAACAGGACCTAAACTGGGCAGGAAAGATGAAGTAGCCAGAAGGCACAGGGTCTAGAACCAGTTTTGTGGCC
1441 ATGGTTCAAGTTGAAAAACCATAGCGTGTTCATGTGTTGTTCCATGGCTCTCCCAGAGTGGACAGATGTGCCTTTCCCTC
1521 CATCGACTGCCTTCAAATCACATTCTTTTCCAATTCTTAAAAGAAGAAATTCAAAACTCAAAAGCAGTCCAAATATTATA
1601 TATATGCATGTGTATATATATATATATATATGCATGTGTATATATATATGAAATTTTTTAAATAATTGGGATTTTTTGAA
1681 ACTTAATGTGTTGATTTTTTTACACCATTGCTGTGCTTTTTGAATATGAAGGATTATTTAAATGAACAACCTTCGTACCA
1761 AAAAAGTCCCTTGGCATTTGCACGTGTGGTCCTTATGGTCTCGTTGTTTATTAGTTTTTTAAATTAGCATGCAGAATAGA
1841 GTTATGGCATTTCCATACAGAGTTTTTGTTGCTGTTCCCCTCTGCCCTCCTGAAGCTTATTCCAAGCATAGGCAAAGGTA
1921 CCTTCCGCTGCTGGAAGCTCCCTGCCCCGGCACACAGCAGGGGTCAGGTCATGGTGTTCAAGCCTCTTTGATTGAAGGTC
2001 CTTGGTTGTTTAACACTGGCCGGTGGAGGCTTAACTCACTCTACACCCAGCTATAAATCAGTCATCATAGAAGCAGTTAG
2081 GGGCAGTTACTGCTATGAGCTGTCAGGAGTATTTGTTTCTGTTTGTATTTTAATGTTTATAGATGGGGAGGAGCAGGGCT
2161 TGTTTTCCTATACTGCAAAGGTCCTTAAGAAAGTCCCAAAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT
2241 CTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACAACCATCTCTGAAGAAAGTTGCAAAGAATAGAGTCCAAAGGCTA
2321 GCTAGCATCACTTGTGGCAAAAGCCAGCACCCTTGATGTCATTCTAAGATGTGCATCTAACACACCTGCAGGGTGCCACC
2401 TGGAGATGCAGCTATGTGACCCACCCGTGTGGAAAAGGATCCCCAAATAGAACCTGGGAGACTCTGAGCCCCCTCCCTCA
2481 CCCTGCAGTGGGACTTCTGGTCATGTTGTCTCCTGCATCCTCCCTCCCCGGGCACGTGGTGCCTCCGTGCCATGCAGAGC
2561 CACCACCCCCAGCTCCCAGAATGTGGGGACAACACCCAGCCTTTGTGCGTTTTCTGGGCTGGCATTCAAAGGCAGCCCAT
2641 GAAGAATGCTCTGAGAAGGCATATTCTTCCAAGGTCTTTTTGCTGTGCGCATGCTCCTTGGGAAGTTCATTTACTGTGCA
2721 CATGTTCCTTGGGAAGAAAGGGAGGCTGTGCTGTGGCCTCTAAGGAACGGGTTTCCCAGCCTGTAGAATGCCCAGGCCTT
2801 ATGAGGCTGTGAGGTTATGGCTTTGGGGTAAAGAATAAAGACACCTGCTCCGAGCCCATTAACTCTCGCCCAGTCCCAGG
2881 ACAGTCCCCAGGCCACCAGCCAGCGTCTCAGAACACAAATCATAGGAAGGTTCCTCTTCCTACCCAGAGCAAGTAGCATC
2961 CGACGGAGGACCTAAGCTGATCATGTAGTGTCACATCCAGACAACACAGGTGCCACAAGAAAACATCTTCCAGTCTCCCA
3041 GGAGGGGGAGGGGCACGCTCTTCACCCGTATCTGTTTGCCTCTATTTATTTTACCTGAGATCTCGGTTTCTAACCCGTTA
3121 GAGCCAGCATTTCAGTCAGTCTTCAGATGGCCGCTCCGCCTCTAATTTTGACAGGATGGGTTTAAGATGCTTCCGGCCAC
3201 TAAGTCCTGGGTGATCTTGTAAATTAGCTAGAACATCTTTTTTCCTGATACTGTCCCATGGCATCCTAAGTTGTGTTAGA
3281 TGCATCTCCGTTCTGCAGTTAGTTTTGTCTTCACTGTTACCGTGGCAACGGAAGGCTCCAGATAAACTTGCCATCCATCC
3361 TGTGAAAGGTGACATTAAAGGTCACATGAAGGACACAGTGGAAGTGCCCAAGAAGTACTCCCAGGGTTCCAGTCTAGTGC
3441 CACCGTCATTGTCATTGCCTTCAGATAAGAGAGAACTCAGGGATCCGGGCAGAAGCAAAGAGAACTAGGGACCCACACAC
3521 CACTCCCTGCCTCCAGTCCACTTGCCAAGAGTTTCCCAGAGACTCTCTGGAGAGAGTCAGCCCGGGATGGACACACTAAC
3601 ATCTGTGTCCAAGTTCACACTTTTGAGATTTCTCGTGTGAATCAGAGTAGCCTCACTTGGTCAGTTCTGGGTCCCTGGTC
3681 TCTGACTCTGCCTTTCTGCTGCCTCGGCCGGTCGGCACAGCCATCCACTGACACTGGAATAGGAATGTGATGGGCGTCGC
3761 ACCCTCTGTAAATGTGGGAATGTTTGTAACTTGTGTTTGTATCTAATGTCGATCCCCTTAGGTGGTTGTACTATAATTCA
3841 TTTTTGTTGTAAACTTCAGCCTAAGATAAATGTACATCTGCTTTTGTATGCACTCATTAAACATTGTAACAGACCAAAGA
3921 TGGTGTACTAATTGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugUUGUUUUAGUGAUCAGAAGGu 5'
            |:||::   :|| ||:|||| 
Target 5' ggAGCAGG---GCTTGTTTTCCt 3'
 2150 - 2169 138.00 -11.20
2 
miRNA  3' uguuGUUUU--AGUGA-UCAGAAGgu 5'
              ||: |  ||| | |||||||  
Target 5' gagcCAGCATTTCAGTCAGTCTTCag 3'
 3121 - 3146 137.00 -15.40
3 
miRNA  3' ugUUGUU-UUAGUGAUCAGAAGGu 5'
            || |: | | |||: |||||| 
Target 5' ttAAGAGCACTGACTGCTCTTCCa 3'
 2223 - 2246 133.00 -13.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
41 mmu-miR-7a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003971 Hells helicase, lymphoid specific 2 1
MIRT053031 Eif4e eukaryotic translation initiation factor 4E 2 1
MIRT053032 Rps6kb1 ribosomal protein S6 kinase, polypeptide 1 2 1
MIRT053033 Mapkap1 mitogen-activated protein kinase associated protein 1 2 1
MIRT053034 Mknk2 MAP kinase-interacting serine/threonine kinase 2 2 1
MIRT053035 Mknk1 MAP kinase-interacting serine/threonine kinase 1 2 1
MIRT577318 Zfp26 zinc finger protein 26 1 3
MIRT578104 Nup133 nucleoporin 133 1 1
MIRT579456 Acsm2 acyl-CoA synthetase medium-chain family member 2 1 1
MIRT581045 Sike1 suppressor of IKBKE 1 1 1
MIRT582290 Nav2 neuron navigator 2 1 3
MIRT583587 Fat3 FAT atypical cadherin 3 1 1
MIRT584159 Creb1 cAMP responsive element binding protein 1 1 1
MIRT586660 Mbp myelin basic protein 1 1
MIRT587038 Milr1 mast cell immunoglobulin like receptor 1 1 1
MIRT587579 Cpm carboxypeptidase M 1 1
MIRT587868 Btn1a1 butyrophilin, subfamily 1, member A1 1 3
MIRT589879 Hdac7 histone deacetylase 7 1 1
MIRT590087 Evi2b ecotropic viral integration site 2b 1 1
MIRT590957 Stxbp2 syntaxin binding protein 2 1 1
MIRT591080 Polr2l polymerase (RNA) II (DNA directed) polypeptide L 1 1
MIRT591342 Gla galactosidase, alpha 1 1
MIRT593125 Cry2 cryptochrome 2 (photolyase-like) 1 1
MIRT594875 Magt1 magnesium transporter 1 1 1
MIRT598790 Gmeb1 glucocorticoid modulatory element binding protein 1 1 1
MIRT599234 Dcaf10 DDB1 and CUL4 associated factor 10 1 1
MIRT600587 Nipal4 NIPA-like domain containing 4 1 1
MIRT600617 Mtfmt mitochondrial methionyl-tRNA formyltransferase 1 1
MIRT600913 Faim2 Fas apoptotic inhibitory molecule 2 1 1
MIRT600985 Ddx27 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 1 1
MIRT602025 Moap1 modulator of apoptosis 1 1 1
MIRT604224 Clca2 chloride channel accessory 3A2 1 1
MIRT604422 Acmsd amino carboxymuconate semialdehyde decarboxylase 1 1
MIRT604505 Trappc13 trafficking protein particle complex 13 1 1
MIRT731319 Sp1 trans-acting transcription factor 1 1 1
MIRT731320 Parp1 poly (ADP-ribose) polymerase family, member 1 1 1
MIRT731919 Nlrp3 NLR family, pyrin domain containing 3 1 1
MIRT732184 Herpud2 HERPUD family member 2 3 1
MIRT734898 Pax6 paired box 6 3 0
MIRT734899 Neurog2 neurogenin 2 3 0
MIRT734900 Neurod1 neurogenic differentiation 1 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-7a Isoproterenol approved 3779 Quantitative real-time PCR heart 22847192 2012 up-regulated
miR-7a Chaihu Shugan San NULL NULL Microarray hippocampus 23947143 2013 up-regualted
miR-7a Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-7a-5p Gefitinib 123631 NSC715055 approved sensitive Low Non-Small Cell Lung Cancer tissue and cell line

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