miRTarBase - #MIRT586666


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pre-miRNA Information
pre-miRNA mmu-mir-674   
Genomic Coordinates chr2: 117185127 - 117185226
Synonyms Mirn674, mmu-mir-674, Mir674
Description Mus musculus miR-674 stem-loop
Comment Landgraf et al. show that the 5' miRNA product is the predominant one .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-674-5p
Sequence 25| GCACUGAGAUGGGAGUGGUGUA |46
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Mbp   
Synonyms C76307, Hmbpr, R75289, golli-mbp, jve, mld, shi
Description myelin basic protein
Transcript NM_001025245   
Other Transcripts NM_001025251 , NM_001025254 , NM_001025255 , NM_001025256 , NM_001025258 , NM_001025259 , NM_010777   
Expression
Putative miRNA Targets on Mbp
3'UTR of Mbp
(miRNA target sites are highlighted)		 
>Mbp|NM_001025245|3'UTR
   1 AGAAGCTGTGGGTTTAAATGCGGAAAGGAAGGAGAGGTGGGCAGGTGGAACTGGGCCAGTCACCATCGCAGAGCAACCGT
  81 GTCCCTGTGTCTGAGATACTAGCCCTGATCGGACAGTGCTGATGGCCTGGGGGATTGGGCGTGGAGTGGGCGTGGTCGCC
 161 ACTTAGCCAATTTCTACTTGTTTTGCTCCCTTTGTCCTTCTCACACGGGACTGCGATTGGGTGTCACTCAGGAATGCAAT
 241 GCCTTCTGCATCCTAACTTGAGGCACCCAGGATGTAGCATTGAGCCTATGGGTTCTTAGGTATACATGCCCTATGCCTGG
 321 GGCTTCAGCAGATCCTGCCTCTGCGCAGTGCTTCCGGGTTTCGAACCTCAGCTGTGCTCATTCTCTGCTGGGTACAGGCT
 401 GCTAATTGCTTGCCGCTGTGAAACAATGGTCCAATTGAGGCGAGGAGGTCTGCCGACTGACCTCTGCACCTCCAGCAAAC
 481 TTTCCTCTTTGTCCTATGTAGTTTGGTGGGGGTGAATGAATGAATGGTGTTCATGACAAAAAAAAAATGTGTGATTCCGT
 561 TAATTTAAATATAACTTGTGTTTCATGATGTTTCAAAAGCAAATGCCCTGTGTTAAAATGTATCAGAAGATAAAATTGTG
 641 CAGATGGTCTAGTTTCCAAGGGCAACATCTGGGGTGGGTTACTCATGAGCCCGTGGTTCAGGGTTTGTCTGTCAGGTGGC
 721 TGCCACCTTGTAAGTGTATGGCATTTTTCCATCTCTCTGTTGGATAGCTGAGCAACTCTTGGGAGAGATATGGATGGGCC
 801 ATTAAAACAAGCCCAGGTCTTTGAGTACCCTCTCAGCCTGGGCAACTTTTCTGCCTGTAAGTTGTGTCTGTTGTCAAAAC
 881 TCTGGCGAACGCAAGAGGCTTTTGACTGAGATGTAGGGCAGAGGCCCCTCCACGGCTCTAGGGCCATTGGCAGTGCAGTT
 961 TTGAAAATGCTGTAGTCTCCAGCCACACCTTTGAGCGTGGGGATGGTTGTGAAATGCTGTACCTAATGACGGCTCTCCTG
1041 CCCCGTGGCCACCAGAGCCCCTTGGTGTCTCAGCAGCAGTCTGGCCCTCGCCTTGGATGTCAGCGCTACTTGTCATTAAC
1121 ACTGGATATGTCTCACTTGCTAACAGAATTCAAGCTGCCCCAGATTCCTGCAGTCTGTACTTAGCGGTATCGCCTTCAGC
1201 CTGATCCATCAATCTTGTTTCCAGGGCCGATTTACATTGTAGTGGCTTCCAAGCACTCATCTCGAGGGAGGCGCTTGAGA
1281 TCTGGAGGATCCTGACTGCCCCCTGAGGTCCATCTGTGTAGCCAAGCCCACACATGCACACAGCCGCACCCAGTCCAGGC
1361 AGCCGAAGGAACAGGACCTAAACTGGGCAGGAAAGATGAAGTAGCCAGAAGGCACAGGGTCTAGAACCAGTTTTGTGGCC
1441 ATGGTTCAAGTTGAAAAACCATAGCGTGTTCATGTGTTGTTCCATGGCTCTCCCAGAGTGGACAGATGTGCCTTTCCCTC
1521 CATCGACTGCCTTCAAATCACATTCTTTTCCAATTCTTAAAAGAAGAAATTCAAAACTCAAAAGCAGTCCAAATATTATA
1601 TATATGCATGTGTATATATATATATATATATGCATGTGTATATATATATGAAATTTTTTAAATAATTGGGATTTTTTGAA
1681 ACTTAATGTGTTGATTTTTTTACACCATTGCTGTGCTTTTTGAATATGAAGGATTATTTAAATGAACAACCTTCGTACCA
1761 AAAAAGTCCCTTGGCATTTGCACGTGTGGTCCTTATGGTCTCGTTGTTTATTAGTTTTTTAAATTAGCATGCAGAATAGA
1841 GTTATGGCATTTCCATACAGAGTTTTTGTTGCTGTTCCCCTCTGCCCTCCTGAAGCTTATTCCAAGCATAGGCAAAGGTA
1921 CCTTCCGCTGCTGGAAGCTCCCTGCCCCGGCACACAGCAGGGGTCAGGTCATGGTGTTCAAGCCTCTTTGATTGAAGGTC
2001 CTTGGTTGTTTAACACTGGCCGGTGGAGGCTTAACTCACTCTACACCCAGCTATAAATCAGTCATCATAGAAGCAGTTAG
2081 GGGCAGTTACTGCTATGAGCTGTCAGGAGTATTTGTTTCTGTTTGTATTTTAATGTTTATAGATGGGGAGGAGCAGGGCT
2161 TGTTTTCCTATACTGCAAAGGTCCTTAAGAAAGTCCCAAAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT
2241 CTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACAACCATCTCTGAAGAAAGTTGCAAAGAATAGAGTCCAAAGGCTA
2321 GCTAGCATCACTTGTGGCAAAAGCCAGCACCCTTGATGTCATTCTAAGATGTGCATCTAACACACCTGCAGGGTGCCACC
2401 TGGAGATGCAGCTATGTGACCCACCCGTGTGGAAAAGGATCCCCAAATAGAACCTGGGAGACTCTGAGCCCCCTCCCTCA
2481 CCCTGCAGTGGGACTTCTGGTCATGTTGTCTCCTGCATCCTCCCTCCCCGGGCACGTGGTGCCTCCGTGCCATGCAGAGC
2561 CACCACCCCCAGCTCCCAGAATGTGGGGACAACACCCAGCCTTTGTGCGTTTTCTGGGCTGGCATTCAAAGGCAGCCCAT
2641 GAAGAATGCTCTGAGAAGGCATATTCTTCCAAGGTCTTTTTGCTGTGCGCATGCTCCTTGGGAAGTTCATTTACTGTGCA
2721 CATGTTCCTTGGGAAGAAAGGGAGGCTGTGCTGTGGCCTCTAAGGAACGGGTTTCCCAGCCTGTAGAATGCCCAGGCCTT
2801 ATGAGGCTGTGAGGTTATGGCTTTGGGGTAAAGAATAAAGACACCTGCTCCGAGCCCATTAACTCTCGCCCAGTCCCAGG
2881 ACAGTCCCCAGGCCACCAGCCAGCGTCTCAGAACACAAATCATAGGAAGGTTCCTCTTCCTACCCAGAGCAAGTAGCATC
2961 CGACGGAGGACCTAAGCTGATCATGTAGTGTCACATCCAGACAACACAGGTGCCACAAGAAAACATCTTCCAGTCTCCCA
3041 GGAGGGGGAGGGGCACGCTCTTCACCCGTATCTGTTTGCCTCTATTTATTTTACCTGAGATCTCGGTTTCTAACCCGTTA
3121 GAGCCAGCATTTCAGTCAGTCTTCAGATGGCCGCTCCGCCTCTAATTTTGACAGGATGGGTTTAAGATGCTTCCGGCCAC
3201 TAAGTCCTGGGTGATCTTGTAAATTAGCTAGAACATCTTTTTTCCTGATACTGTCCCATGGCATCCTAAGTTGTGTTAGA
3281 TGCATCTCCGTTCTGCAGTTAGTTTTGTCTTCACTGTTACCGTGGCAACGGAAGGCTCCAGATAAACTTGCCATCCATCC
3361 TGTGAAAGGTGACATTAAAGGTCACATGAAGGACACAGTGGAAGTGCCCAAGAAGTACTCCCAGGGTTCCAGTCTAGTGC
3441 CACCGTCATTGTCATTGCCTTCAGATAAGAGAGAACTCAGGGATCCGGGCAGAAGCAAAGAGAACTAGGGACCCACACAC
3521 CACTCCCTGCCTCCAGTCCACTTGCCAAGAGTTTCCCAGAGACTCTCTGGAGAGAGTCAGCCCGGGATGGACACACTAAC
3601 ATCTGTGTCCAAGTTCACACTTTTGAGATTTCTCGTGTGAATCAGAGTAGCCTCACTTGGTCAGTTCTGGGTCCCTGGTC
3681 TCTGACTCTGCCTTTCTGCTGCCTCGGCCGGTCGGCACAGCCATCCACTGACACTGGAATAGGAATGTGATGGGCGTCGC
3761 ACCCTCTGTAAATGTGGGAATGTTTGTAACTTGTGTTTGTATCTAATGTCGATCCCCTTAGGTGGTTGTACTATAATTCA
3841 TTTTTGTTGTAAACTTCAGCCTAAGATAAATGTACATCTGCTTTTGTATGCACTCATTAAACATTGTAACAGACCAAAGA
3921 TGGTGTACTAATTGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auguggugaggguaGAGUCACg 5'
                        ||||||| 
Target 5' ggctggagagatggCTCAGTGg 3'
 2201 - 2222 140.00 -10.50
2 
miRNA  3' auGUGGUGAGGG-UAGA-GUCAcg 5'
            |||||||||| : || ||||  
Target 5' caCACCACTCCCTGCCTCCAGTcc 3'
 3516 - 3539 128.00 -26.80
3 
miRNA  3' auGUGGUGAGG--GUAGAGUCacg 5'
            |||||  ||  |:||||||   
Target 5' gcCACCA-GCCAGCGTCTCAGaac 3'
 2892 - 2914 125.00 -18.92
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' auguggugaggguaGAGucacg 5'
                        |||     
Target 5' ggcuggagagauggCUC----- 3'
 		3 - 19
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
94 mmu-miR-674-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577119 Prps2 phosphoribosyl pyrophosphate synthetase 2 1 3
MIRT579212 Ccdc25 coiled-coil domain containing 25 1 4
MIRT580735 Srp72 signal recognition particle 72 1 2
MIRT581673 Ppp2r2c protein phosphatase 2, regulatory subunit B, gamma 1 1
MIRT582930 Il15ra interleukin 15 receptor, alpha chain 1 1
MIRT584530 Bzrap1 TSPO associated protein 1 1 1
MIRT584648 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT586666 Mbp myelin basic protein 1 2
MIRT588993 Rpl7l1 ribosomal protein L7-like 1 1 1
MIRT589588 Magt1 magnesium transporter 1 1 1
MIRT590893 Tmem69 transmembrane protein 69 1 2
MIRT590965 Slfn5 schlafen 5 1 2
MIRT591089 Pola2 polymerase (DNA directed), alpha 2 1 2
MIRT591253 Loxl2 lysyl oxidase-like 2 1 2
MIRT591405 Churc1 churchill domain containing 1 1 2
MIRT591425 Casc4 cancer susceptibility candidate 4 1 2
MIRT591686 Slc23a1 solute carrier family 23 (nucleobase transporters), member 1 1 2
MIRT591985 A230046K03Rik WASH complex subunit 4 1 2
MIRT593044 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 2
MIRT593330 Ddx3x DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked 1 1
MIRT594701 Anks1b ankyrin repeat and sterile alpha motif domain containing 1B 1 1
MIRT595566 Sh3rf3 SH3 domain containing ring finger 3 1 1
MIRT595693 Pcsk6 proprotein convertase subtilisin/kexin type 6 1 1
MIRT595863 Myh10 myosin, heavy polypeptide 10, non-muscle 1 1
MIRT596037 Ubp1 upstream binding protein 1 1 1
MIRT596075 Ywhag tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 1 1
MIRT596182 Med22 mediator complex subunit 22 1 1
MIRT596482 Zyg11b zyg-ll family member B, cell cycle regulator 1 1
MIRT596495 Zfp935 zinc finger protein 935 1 1
MIRT596811 Tyk2 tyrosine kinase 2 1 1
MIRT596873 Tspan15 tetraspanin 15 1 1
MIRT597076 Tbc1d24 TBC1 domain family, member 24 1 1
MIRT597180 Spc24 SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) 1 1
MIRT597235 Slc7a14 solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 1 1
MIRT597281 Slc22a15 solute carrier family 22 (organic anion/cation transporter), member 15 1 1
MIRT597347 Scd3 stearoyl-coenzyme A desaturase 3 1 1
MIRT597359 Sap18 Sin3-associated polypeptide 18 1 1
MIRT597446 Rin1 Ras and Rab interactor 1 1 1
MIRT597514 Rbbp4 retinoblastoma binding protein 4, chromatin remodeling factor 1 1
MIRT597557 Rab33b RAB33B, member RAS oncogene family 1 1
MIRT597808 Pla2g4b phospholipase A2, group IVB (cytosolic) 1 1
MIRT597974 Nup133 nucleoporin 133 1 1
MIRT598205 Mpv17l Mpv17 transgene, kidney disease mutant-like 1 1
MIRT598257 Mfsd2b major facilitator superfamily domain containing 2B 1 1
MIRT598574 Irak1 interleukin-1 receptor-associated kinase 1 1 1
MIRT598860 Gm10778 predicted gene 10778 1 1
MIRT599029 Fam177a family with sequence similarity 177, member A 1 1
MIRT599181 Dennd5b DENN/MADD domain containing 5B 1 1
MIRT599224 Dcaf4 DDB1 and CUL4 associated factor 4 1 1
MIRT599261 D3Ertd254e DNA segment, Chr 3, ERATO Doi 254, expressed 1 1
MIRT599278 Cyp2e1 cytochrome P450, family 2, subfamily e, polypeptide 1 1 1
MIRT599286 Cxcr2 chemokine (C-X-C motif) receptor 2 1 1
MIRT599294 Cx3cr1 chemokine (C-X3-C motif) receptor 1 1 1
MIRT599328 Comt catechol-O-methyltransferase 1 1
MIRT599355 Commd7 COMM domain containing 7 1 1
MIRT599488 Ccr9 chemokine (C-C motif) receptor 9 1 1
MIRT599583 Brip1 BRCA1 interacting protein C-terminal helicase 1 1 1
MIRT599647 Banp BTG3 associated nuclear protein 1 1
MIRT599683 Arl16 ADP-ribosylation factor-like 16 1 1
MIRT599726 Ankfy1 ankyrin repeat and FYVE domain containing 1 1 1
MIRT600098 1700047I17Rik2 RIKEN cDNA 1700047I17 gene 2 1 1
MIRT601167 Atrn attractin 1 1
MIRT601187 Asxl2 additional sex combs like 2 (Drosophila) 1 1
MIRT601347 Zfp202 zinc finger protein 202 1 1
MIRT601378 Wdr89 WD repeat domain 89 1 1
MIRT601554 Tapbpl TAP binding protein-like 1 1
MIRT601574 Ssr1 signal sequence receptor, alpha 1 1
MIRT601739 Rbm34 RNA binding motif protein 34 1 1
MIRT601844 Polr2l polymerase (RNA) II (DNA directed) polypeptide L 1 1
MIRT601894 Pbld1 phenazine biosynthesis-like protein domain containing 1 1 1
MIRT601949 Nol3 nucleolar protein 3 (apoptosis repressor with CARD domain) 1 1
MIRT602826 AK010878 GON7, KEOPS complex subunit homolog 1 1
MIRT602859 Rmi2 RecQ mediated genome instability 2 1 1
MIRT602916 1700016D06Rik RIKEN cDNA 1700016D06 gene 1 1
MIRT603001 Myo1c myosin IC 1 1
MIRT603576 Ppm1k protein phosphatase 1K (PP2C domain containing) 1 1
MIRT603581 Ppil4 peptidylprolyl isomerase (cyclophilin)-like 4 1 1
MIRT603702 Nip7 NIP7, nucleolar pre-rRNA processing protein 1 1
MIRT603710 Naip5 NLR family, apoptosis inhibitory protein 5 1 1
MIRT603942 H2-T23 histocompatibility 2, T region locus 23 1 1
MIRT604103 Dph5 diphthamide biosynthesis 5 1 1
MIRT604202 Cpne9 copine family member IX 1 1
MIRT604229 Clca2 chloride channel accessory 3A2 1 1
MIRT604242 Cflar CASP8 and FADD-like apoptosis regulator 1 1
MIRT604392 Aipl1 aryl hydrocarbon receptor-interacting protein-like 1 1 1
MIRT604542 Ypel2 yippee-like 2 (Drosophila) 1 1
MIRT605321 Zfp605 zinc finger protein 605 1 1
MIRT605421 T2 brachyury 2 1 1
MIRT605424 Synrg synergin, gamma 1 1
MIRT605630 Mfsd11 major facilitator superfamily domain containing 11 1 1
MIRT605750 Exosc2 exosome component 2 1 1
MIRT605832 Cenpf centromere protein F 1 1
MIRT606053 Zfp607 zinc finger protein 607A 1 1
MIRT606349 Lcmt2 leucine carboxyl methyltransferase 2 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-674 Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-674 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated

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