miRTarBase - #MIRT586765


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pre-miRNA Information
pre-miRNA mmu-mir-137   
Genomic Coordinates chr3: 118433857 - 118433929
Synonyms Mirn137, mmu-mir-137, Mir137
Description Mus musculus miR-137 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-137-3p
Sequence 45| UUAUUGCUUAAGAAUACGCGUAG |67
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B41RDC miR-137 Safety Biomarker (SAF) Clinical/Experimental Data Expression Decrease Neuron Reverse transcription-polymerase chain reaction
Gene Information
Gene Symbol Kcnk6   
Synonyms D7Ertd764e, Toss, Twik2
Description potassium inwardly-rectifying channel, subfamily K, member 6
Transcript NM_001033525   
Expression
Putative miRNA Targets on Kcnk6
3'UTR of Kcnk6
(miRNA target sites are highlighted)		 
>Kcnk6|NM_001033525|3'UTR
   1 CTGTCGACCTGGACACACCTGGGCAGGGGCTTTGAGCCCCATGTGACTAGATCAACTGGAATGCCCAGACACACTCTCCT
  81 GTCTTTTCACAGAGCTCCACTGGGAGGTGAGGAGTAGTCCCTCTGCTCCTATGTCCTCTGCTCCTCAGGGCACTGACACA
 161 GTCATTTTCTCCTCCTTCCTTTTCTCCCTTTTCTTCTCAGACTCAGACCACGTGGGCTTTCTGACTTTGTTGGTGAGAGT
 241 AGTGGTTTATACAATTTTTTTGTTTTGTTTTGTTCTTGTTGTTTTTCTATGTCTCGCTCTCTTGCATAGCTCATCCGGCA
 321 CACCTAGTTCTTCTGGGCTCTGGGCCTGGACTCCACTTTAGCCTCTGTATTAGTGGCTTTGTCTTGGCCAAATGATTTCC
 401 CCATTCTAAACCTTTATATCCCAGCCATAAGATGCAAAGAATGTTCAAACTCATCCACAGACCTTCACTTCTGCCATGCT
 481 TTTCTGTGCTAGGCACACAGTAGTAGATGTGGCTTGGGCTCTGCTCTTATTGGGGTGACCAGCCAGTGAGGTAAACTGAC
 561 TGACTTGCTGTCCCTTGATGGTGACAAACTCCAGTGGGCACTGGAAGAGGCAGAGTGGTCAGGACTGAGGAGTGGACCTG
 641 AAATGTCCAGGTGGGCTTTCCTGGAAAACGGGACAAGAACTAACCCAAGACCTGCCAGTCAAGAGAAGAAATTCTGCCTT
 721 CTTCTCAGGTGTCTATGGGCCGTAGGGCCTGTGGGAAGAGGCCTGCAAGGGGCCAGCATCTCCCTCAACGACTTAACAGG
 801 CAAGGAACTGACCCAGGATATGAGCCCAGATCCAAGTGGCGTGAGAGGGGTAACTTAGCCTGGTCCTATGGTGATGCTCT
 881 GTGTACCGGGGCACTGACTGATGGCCTTTCTGGGCATGCCCTGTAACTGGGCCATATTTTGCCAATGCATTTGGACAGTC
 961 ACTCTACCTTTGGGACTCTGTGTCTGCTAAATGGGGAATCATAATGCTACTCCAAAAGTTGACATCAACCAGGTGCAGTA
1041 CTGGGTACCAGTAGCCTGAGCTGAAGCAGGAGGCTTGAGAGTTCAAAGGCAGGATGGGATACACAGCAGGAGCCAGGTGA
1121 TAAAATAAGCTAAGCAATATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAATCACAGGAATATAG
1201 TGCCAAGCTAAAGGGGAGTAGGCTACAGAAGGATATACAGTCCTTGGGGTTGGAAATTACTTATACAATTGTATCACTTA
1281 AGGATTTTGACTGCAACTGACAAAAGCCAGACTGAAACAACCTGATTGTTCAATTGTAACTGGACAGAACCCGGGTGGGG
1361 AGAGACTGAGCTTGATGCTCTGCATGGCTACAGAAGGGGTGGTGGCCGTGGGTTCAGCCTCTCTTCTCCCCATAGCAGAC
1441 TTAGCTCTCATATGTGTTCTCCTCCTGGTGTCTAATGGTTGCCACAGACCCAGGCATCGAAACCAGACGTGTTATTTCTA
1521 GTGCAAGCAATAGAAACTTCAAGCAGTATTCTGAGAACTTTAAGGAAGGCTGGCTCAGTACCAAACCACTGTAGAAAGTC
1601 ACACGGGGGAACTGGGTGTGTTGGCATACAGCTACCCTAGCACTGTGGAGGGTGTGCCTGGAGAGTCCTGAGGCTAGGCT
1681 AGCCTGGGCAACCGATAATGACACCACAAAACTCCAAAGGGAGCTAGGAATGTTCACAGTGGTGGAACACGTCTGCCTCA
1761 GGTGCTAAAGAAAACAATTTACAATTATAGGTGCTGGGAAGATGGCTTAGCCAGGCAGGCTCAAAAACCTGAGTTTCTCC
1841 CCAGTCCCTACATAGAAAATGCTAGATGTCATGTCATGGTGCTGCCCTTTAATCCCAGCATCTGGAGGCAGAAGCTGGTA
1921 GAATTTCTGCGAGTTCAAGGCCAGCATGGTCTACTTAGTGAGTTTGAGGACAATTAGGGCTACATAGTAAGACCCTGCAT
2001 CCACAAAATAACTAAAAACTAAAAATAGTGTGACAAGTGATTAGGAAAAAAGTAGATATTAAACTCTAGCTTCCATGTGT
2081 AAACTCACACACACTTAAAAGTCTGAGGCAGCAAAATATTTTTTTGGCGGGGGGCTGGTTTCAAGACAGGGTTTCTCTAT
2161 GTAGCCCTGGCTATCCTGGAACTCACTTTGTAGACCAGGCTGGCCTTGAACTCAGAAATCTGCCTGCCTCTGCTTCCTAA
2241 GTGCTGGGATTAAAGGTATGCACCACCACACCCGGCTTCATAATGAGTTATTTCTCTACCAGTGAAATGAGTCAGTTCAA
2321 GCCAGCTTGGATTATATTGAAGGCAGGTTTATTGGGAAACTGGCCTTGGTTGAGTTCACTGGCCCCAAGGATTGAGCCCA
2401 GGGGAATTGCCATTGGAGGTGGTATGGGGAGAAAAGACACATGTGCACACATACACAGGGGGAAAGACAGATGTCTGAAT
2481 TATATAGGGAGGAGCCTGGGGGGGGGGGGGAGAACAGCCCAGCCCCTGAGCTAAAAAGTTCAAGCTTGGGGTCAGGATAT
2561 GTCAGGTAGGGACTGAGGGATGCTGGGAGAACCTGGAGGCCAGGTCTGCTCTGATATGTAAAATGCATACCTCAATCCCT
2641 TGTCCCAGGGTCCAAAACCAAAGTACCCAAAGTACAATTGATTTCTCAAGTCTTTATCTGCATATAGGCTATGCACGAAC
2721 TAGCAATATGTGGATGTGCATATGTGTGAGTCTTCCACCCTACACAAAGGTTACCAGCAATGGGAAGTGATGACTATTCC
2801 TGGTTCTGAAAGCAGAACTCTGTGAGTTTGAACCCTTGTCCTTGTATTTCACTTGTGCCTCAGATTGCTTATTTATGAAA
2881 GGGTATTTGTACAGCACTACCTCGTGGACTGGAACACCCAGCCGTCTAATGCTTGTAACTCTTACCCTACAGCACCATCA
2961 GGGAGTCAATAAAGTGCCTGACCCGTCCCTCGATCTGAACTCTGTTCTTAGCAAGGAAGGTCACGTAAAATAGAAATGCG
3041 TGACTTTTGGGATAGCATTGGAAATGTAAATGAAGAAAATACCTAATTAAAAAAAAAAAGAAATGCGT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gaUGCGC-AU-AAGA--A--UUCGUUAUu 5'
            |||:| || ||||  |  |||||||| 
Target 5' agACGTGTTATTTCTAGTGCAAGCAATAg 3'
 1505 - 1533 165.00 -16.30
2 
miRNA  3' gaugcgcAUAAGAAUUCGUUAUu 5'
                 ||  | ||||||||| 
Target 5' gataaaaTAAGC-TAAGCAATAt 3'
 1119 - 1140 151.00 -7.20
3 
miRNA  3' gaUGCGCAUAAGAAUUCGUUAUu 5'
            |:||  :  ||  ||||||| 
Target 5' ctATGCACGAACT--AGCAATAt 3'
 2709 - 2729 145.00 -10.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
16 mmu-miR-137-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000452 Ezh2 enhancer of zeste 2 polycomb repressive complex 2 subunit 4 1
MIRT001015 CDK6 cyclin dependent kinase 6 3 1
MIRT005501 Tbx3 T-box 3 3 1
MIRT006933 Nf1 neurofibromin 1 1 1
MIRT014840 Cdk6 cyclin-dependent kinase 6 1 1
MIRT438758 Nr1i3 nuclear receptor subfamily 1, group I, member 3 2 1
MIRT580289 Trhr thyrotropin releasing hormone receptor 1 1
MIRT581519 Ptprd protein tyrosine phosphatase, receptor type, D 1 1
MIRT585929 Slc16a9 solute carrier family 16 (monocarboxylic acid transporters), member 9 1 1
MIRT586765 Kcnk6 potassium inwardly-rectifying channel, subfamily K, member 6 1 1
MIRT593788 Ncf2 neutrophil cytosolic factor 2 1 1
MIRT594208 Wdr12 WD repeat domain 12 1 1
MIRT595898 Cd69 CD69 antigen 1 1
MIRT596006 Diras2 DIRAS family, GTP-binding RAS-like 2 1 1
MIRT597415 Rpp40 ribonuclease P 40 subunit 1 1
MIRT604053 Esf1 ESF1 nucleolar pre-rRNA processing protein homolog 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-137 Curcumin NULL 969516 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-137 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-137 Emodin NULL 3220 Microarray K562 cells 23744534 2013 down-regualted
miR-137 Emodin NULL 3220 Quantitative real-time PCR K562 cells 23744534 2013 down-regualted
miR-137 Olea europaea leaf extract NULL NULL Quantitative real-time PCR Glioblastoma multiforme tumors cell lines 25232498 2014 up-regualted
miR-137 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-137 Ginsenoside Rh2 NULL 119307 Microarray human glioma cells U251 21372826 2011 up-regulated
miR-137 Interleukin 13 (IL-13) NULL NULL Quantitative real-time PCR esophageal squamous cells 22453679 2012 up-regulated
miR-137 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-137 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated
miR-137 Olea europaea leaf extract NULL NULL Quantitative real-time PCR glioblastoma cells. 22722712 2012 up-regulated
miR-137 Temozolomide approved 5394 Quantitative real-time PCR glioblastoma cells. 22722712 2012 up-regulated
miR-137 Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 up-regulated
miR-137 Capecitabine approved 60953 Quantitative real-time PCR rectal cancer 18695884 2008 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-137-3p Doxorubicin 31703 NSC123127 approved sensitive Low Neuroblastoma tissue and cell line (HepG2, LS174T, UKF-NB3)

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