miRTarBase - #MIRT587538


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miRNA Infomation
miRNA namemmu-miR-1186b

Gene Information
Gene Symbol Cxcr2   
Synonyms CD128, CDw128, Cmkar2, Gpcr16, IL-8Rh, IL-8rb, IL8RA, Il8rb, mIL-8RH
Description chemokine (C-X-C motif) receptor 2
Transcript NM_009909   
Expression
Putative miRNA Targets on Cxcr2
3'UTR of Cxcr2
(miRNA target sites are highlighted)		 
>Cxcr2|NM_009909|3'UTR
   1 AGACTGTTCACCTAAACGGTGGCCCCTCGGGGTTCCTTCTTGTCTTTCAGCATGGCTCATTACCAGAGACTGTGGTATTT
  81 GAATTGATGCAGCCCCTCCTCTACAATTACAGGGAGAAAGAGGTCACGTTCTTAGCAGAGCCCCCAGAGTTTAGAACCCC
 161 CTATATTGGCTGTCTGCTCCCTTCCATCTTGGTATGCCTACTGATAGAGTTGATCCATCCTAACACTAGACCCCAAACAC
 241 TCTTTTCTAAGAAGCACACGTTACAATTACAGTGAGATACTGCCTCCTACCCATCAGAACAGTTAGCAGTAAAAGGAAGA
 321 GGTGGAGGAGAGAAAATGGCAAGTGACAATGAGGAAGTAGAAAAAGGGAACTCTTCACCTTACCAGTAGACGAGTACCAG
 401 AGTCCCCTCACACAGGAACATAGCATAGCAGTTTTCCATTTAAAAAAAAATTAAGGGCTGGTGAGATGGCTCAGTGGGTA
 481 AGAGCACCCGACTGCTCTTCCAAAGGTCCGAAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAACGAG
 561 ATCTGATGCCCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAATAAATCTTTAAAAAAAA
 641 AAATTAAATATAGAATTGGCATGTAATCCAGCAAGTCCATCTCTAAGTAGGGTACCTAAAAAAATTGAAAGCCAGGGTTC
 721 AGACATTTGTATGCTGATACTCACAACAGCATTATTCATCATGAAAATGATGGCCAACAGATGAATGGGTAAGTTAAACA
 801 AGGTGTCTCTACACAACAAAAGAGCGTCCACAATGAGAAGTGCCAAAAATAATCAAATAGTGTTGACTGGAGGCTGGGTG
 881 AGGAAGGGTGGGGAGTTCGTGTAGAAGACACAGCTCCAGTTAGGGATGATGGGAAAGCCCTGAAGGTGGGCCCTGATCCC
 961 TGTGTAGTAGTGTGAATGCACTCATTACCACTGTAACAAAAGTGTTAAAATGAAAGCTGAGTATTGCTCCCTGGGAGAGT
1041 GCTTATTTAACATTTCTAAGAACTCAGGCTCAAATCCTAGCACCTCGACAAACAAACAAACAAACAAACAAAAAAGCTAA
1121 AATGGTTATTTTTGTTATGTGTGTTTTACCTCACTTTACCAAAAATATAAAAATCTAAGTCTAAAAAATGTTCACCATCC
1201 CATAAACAACCACCAAACCCAGACACTAGGCAGATGCCAACAAGAACCTGCTGACAGGAGGCTGATATAGCTGTCTCCTG
1281 AGGAGCTCTGCCAGTGCCTGGAAAATACAGAAGTGGATGCTCACAATCATCCATTGGATAGAGGACAAGGTCCCAAATGA
1361 AGAAGCTAGCGAAGGTATCCAAGGAGCTAAAGGGGTCTGAAGCCCCAGAGGAGGAACATCAGTATGAACTAGCCAGTACC
1441 CCCCCAGAGCTCCTTGGAACTAAACCACCAATCAAAGAAAACACATGGTGGAACTTGTGGCTCTAGCTGTATATGTAGCA
1521 GAGGATGGCCTAGTCAGTCATCAATGGGAGGAGAGGCCCTTGGTCCTGTGAAGGCTCTATGCCTCAATATAGGGGAATGC
1601 CAGGACCAGGAATGGGAGTAGGTGGATTGGGGAGCAGGGGGAGAGGGAAGGGAATAGGGGATATTTGGAGGGGAAACTAG
1681 GAAAGGGGATAACATTTGAAATGTAAATAAAGAAAATATCTAATAAAAAAAATGTTCTTTAACCACTGAACCAATGCAGA
1761 ACTATCTACCTTATAATTTCTTGTAAAGTGTGACTATTAAATGTCGTTTTAAAAAATA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM4751756
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 1
Location of target site NM_009909 | 3UTR | GUGGGUAAGAGCACCCGACUGCUCUUCCAAAGGUCCGAAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4751757
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 2
Location of target site NM_009909 | 3UTR | CUCAGUGGGUAAGAGCACCCGACUGCUCUUCCAAAGGUCCGAAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4751758
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 3
Location of target site NM_009909 | 3UTR | CUCAGUGGGUAAGAGCACCCGACUGCUCUUCCAAAGGUCCGAAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM4751759
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 4
Location of target site NM_009909 | 3UTR | GUGAGAUGGCUCAGUGGGUAAGAGCACCCGACUGCUCUUCCAAAGGUCCGAAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACAACC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM4751760
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 1
Location of target site NM_009909 | 3UTR | GCUCAGUGGGUAAGAGCACCCGACUGCUCUUCCAAAGGUCCGAAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACAAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM4751761
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 2
Location of target site NM_009909 | 3UTR | CUCAGUGGGUAAGAGCACCCGACUGCUCUUCCAAAGGUCCGAAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACAAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 7 for dataset GSM4751763
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 4
Location of target site NM_009909 | 3UTR | CUCAGUGGGUAAGAGCACCCGACUGCUCUUCCAAAGGUCCGAAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 8 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_009909 | 3UTR | AAGUUCAAAUCCCAGCAACCACAUGGUGGCUCACAACCAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
86 mmu-miR-1186b Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT576999 Mark2 MAP/microtubule affinity regulating kinase 2 2 2
MIRT585120 Zfp935 zinc finger protein 935 2 2
MIRT586283 Prc1 protein regulator of cytokinesis 1 2 4
MIRT586381 Pbld1 phenazine biosynthesis-like protein domain containing 1 2 2
MIRT586708 Loxl2 lysyl oxidase-like 2 2 4
MIRT587092 Gla galactosidase, alpha 2 4
MIRT587538 Cxcr2 chemokine (C-X-C motif) receptor 2 2 2
MIRT589503 Myo1e myosin IE 2 4
MIRT589590 Magt1 magnesium transporter 1 2 2
MIRT590954 Stxbp2 syntaxin binding protein 2 2 2
MIRT591055 Rab11fip1 RAB11 family interacting protein 1 (class I) 2 2
MIRT591076 Polr2l polymerase (RNA) II (DNA directed) polypeptide L 2 2
MIRT591337 Gm10778 predicted gene 10778 2 2
MIRT592001 Wdr34 WD repeat domain 34 2 4
MIRT592296 Foxr1 forkhead box R1 2 4
MIRT593950 Cpne1 copine I 2 2
MIRT593956 Cpm carboxypeptidase M 2 2
MIRT595194 Tmtc3 transmembrane and tetratricopeptide repeat containing 3 2 2
MIRT595287 Mef2c myocyte enhancer factor 2C 2 2
MIRT596506 Zfp933 zinc finger protein 933 2 2
MIRT596786 Ubxn2a UBX domain protein 2A 2 2
MIRT597061 Tcea1 transcription elongation factor A (SII) 1 2 2
MIRT597101 Taf1a TATA-box binding protein associated factor, RNA polymerase I, A 2 2
MIRT597153 Stxbp4 syntaxin binding protein 4 2 2
MIRT597459 Rhag Rhesus blood group-associated A glycoprotein 2 2
MIRT597814 Pla2g2d phospholipase A2, group IID 2 2
MIRT597836 Pik3c2a phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha 2 2
MIRT598087 Nek7 NIMA (never in mitosis gene a)-related expressed kinase 7 2 2
MIRT598275 Med16 mediator complex subunit 16 2 2
MIRT598705 Haus2 HAUS augmin-like complex, subunit 2 2 2
MIRT598887 Glrx2 glutaredoxin 2 (thioltransferase) 2 2
MIRT598959 Gemin8 gem nuclear organelle associated protein 8 2 2
MIRT599390 Clmn calmin 2 2
MIRT599417 Chek2 checkpoint kinase 2 2 2
MIRT599571 Btc betacellulin, epidermal growth factor family member 2 2
MIRT599852 Adarb1 adenosine deaminase, RNA-specific, B1 2 2
MIRT599903 Acat1 acetyl-Coenzyme A acetyltransferase 1 2 2
MIRT599922 A830018L16Rik RIKEN cDNA A830018L16 gene 2 2
MIRT599998 4930579G24Rik RIKEN cDNA 4930579G24 gene 2 2
MIRT600123 Zfyve27 zinc finger, FYVE domain containing 27 2 2
MIRT600437 Samd8 sterile alpha motif domain containing 8 2 2
MIRT600468 Rbm4 RNA binding motif protein 4 2 2
MIRT600872 Gabpb2 GA repeat binding protein, beta 2 2 2
MIRT601116 Bri3bp Bri3 binding protein 2 2
MIRT601328 Zfp689 zinc finger protein 689 2 2
MIRT601448 Tprkb Tp53rk binding protein 2 2
MIRT601451 Tns4 tensin 4 2 2
MIRT601483 Tmod1 tropomodulin 1 2 2
MIRT601536 Tet1 tet methylcytosine dioxygenase 1 2 2
MIRT601857 Pkd2 polycystic kidney disease 2 2 2
MIRT601946 Nwd1 NACHT and WD repeat domain containing 1 2 2
MIRT602015 Mpp7 membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) 2 2
MIRT602020 Moap1 modulator of apoptosis 1 2 2
MIRT602190 Hyal1 hyaluronoglucosaminidase 1 2 2
MIRT602217 Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 2 2
MIRT602370 Fbxl3 F-box and leucine-rich repeat protein 3 2 2
MIRT602416 F2r coagulation factor II (thrombin) receptor 2 2
MIRT602429 Ercc4 excision repair cross-complementing rodent repair deficiency, complementation group 4 2 2
MIRT602495 Dkc1 dyskeratosis congenita 1, dyskerin 2 2
MIRT602566 Csf2rb colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) 2 2
MIRT602668 Car5b carbonic anhydrase 5b, mitochondrial 2 2
MIRT602688 Camk4 calcium/calmodulin-dependent protein kinase IV 2 2
MIRT602852 Abcd2 ATP-binding cassette, sub-family D (ALD), member 2 2 2
MIRT602997 Pdzd2 PDZ domain containing 2 2 2
MIRT603145 Ubxn8 UBX domain protein 8 2 2
MIRT603436 Rpl7l1 ribosomal protein L7-like 1 2 2
MIRT603456 Rhbdl2 rhomboid, veinlet-like 2 (Drosophila) 2 2
MIRT603635 Pbxip1 pre B cell leukemia transcription factor interacting protein 1 2 2
MIRT603725 Mrpl51 mitochondrial ribosomal protein L51 2 2
MIRT603750 Mfsd2b major facilitator superfamily domain containing 2B 2 2
MIRT603819 Kif5b kinesin family member 5B 2 2
MIRT603907 Hpgds hematopoietic prostaglandin D synthase 2 2
MIRT603918 Hltf helicase-like transcription factor 2 2
MIRT603975 Gnal guanine nucleotide binding protein, alpha stimulating, olfactory type 2 2
MIRT604114 Diap2 diaphanous related formin 2 2 2
MIRT604187 Cxcl11 chemokine (C-X-C motif) ligand 11 2 2
MIRT604294 Ccdc36 coiled-coil domain containing 36 2 2
MIRT604400 Aida axin interactor, dorsalization associated 2 2
MIRT604629 Syt11 synaptotagmin XI 2 2
MIRT604707 Scml4 sex comb on midleg-like 4 (Drosophila) 2 2
MIRT604967 Gga2 golgi associated, gamma adaptin ear containing, ARF binding protein 2 2 2
MIRT605165 C030039L03Rik zinc finger protein 607B 2 2
MIRT605198 B4galt6 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 2 2
MIRT605619 Mpv17 MpV17 mitochondrial inner membrane protein 2 2
MIRT606468 Tcaim T cell activation inhibitor, mitochondrial 1 1
MIRT606659 Lsm11 U7 snRNP-specific Sm-like protein LSM11 2 2

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