pre-miRNA Information | |
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pre-miRNA | mmu-mir-466e |
Genomic Coordinates | chr2: 10479088 - 10479171 |
Synonyms | mmu-mir-466e, Mir466e |
Description | Mus musculus miR-466e stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-466e-3p |
Sequence | 51| UAUACAUACACGCACACAUAAGA |73 |
Evidence | Experimental |
Experiments | Cloned |
Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | Cxcl5 | ||||||||||||||||||||
Synonyms | AMCF-II, Cxcl6, ENA-78, GCP-2, LIX, Scyb5, Scyb6 | ||||||||||||||||||||
Description | chemokine (C-X-C motif) ligand 5 | ||||||||||||||||||||
Transcript | NM_009141 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Cxcl5 | |||||||||||||||||||||
3'UTR of Cxcl5 (miRNA target sites are highlighted) |
>Cxcl5|NM_009141|3'UTR 1 AAAGATTTCTGAGGACTCTGACCCCAGTGAAGATAAGAAGAAAGGGCTGATTCTCTCCACCCACGGATTTTCTTTATGAA 81 CTCCCTGCTTTGATGAGAAAAGGGAAACCATTGTCCCTGAAGCTTCCTGCTCATCTCACAAGTGTATTTAGCAAAAATAT 161 TTCCTATTCTTTCCTATCTGTCACACTATCGGAAATTGTGATACTTTAGTGTGTAAGTCAAGAATCATTGGTTGTTAACC 241 TTTCAAAATGTCTTGAACTGAAGGTGACTATTGCATTGTCTCCGGAATATGTATATGTATATGTATATGTATATGTATAT 321 GTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGATGTATATGTATATGTATATGTGTG 401 TGTATATATATATATATATCCTTTGTAATGTTCATGAGAAGGCAATGCTGTCATACTCTCTCTCATAAAATGGGGACCTC 481 TTTTACTGTTGTTTCTTGGGTGTGTTAAGAGTGTTCTTACGTCTGAGCCTGGGCTGGCATATAACTTTTATGGTTTTAGA 561 AAGCTGCTGTGTCATGCAGAAACCTATGTAGAACACAGTTCCTTATTTAGAATTTCTAAATGTGTAAGTTCTAGAAGGAT 641 CAATGTGTTCTCATCTTATAATTTTAGACATTTGATGTCTTCGTAGTATGGCATAATGTCATGACTTACTCAACAAACTG 721 TATACTATTTATTAATTATTAACTAGTTTATTAGTATTACTATTTGGGCCACTAACTATATATTTTAGATAGTATATATT 801 TTAGATAGGTGAAGAAGGTAAGAAGCAAGGAAATTCCTTACCTAGTTTTCATAAAAAACATTTAAAAGTTATTTCTAGGG 881 AGAGAAACTTTAGCAAATATCTATACTCTTAGAGTTTTGAAAACATACTTAACATGTTTGAATATGGATTGCTGAGATAT 961 TTAGATGTCTGTAGTGTGTCTTTTAAAGAGTGTGCTTTGTTATAAGGAGATTAATGAATTCATCACATCCATTATTTTAA 1041 GCCTGTACATCTTATAGTGTCTCACTTCTTTATATTACTCTTTTGAGGAAAATAAAGTAGCTCTAGATTCCA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | C2C12 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast
... - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell. |
Article |
- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al. - Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
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CLIP-seq Support 1 for dataset GSM4751756 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of iBAT 1 |
Location of target site | NM_009141 | 3UTR | UAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM4751760 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of eWAT 1 |
Location of target site | NM_009141 | 3UTR | UAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAU |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM1385342 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast |
Location of target site | NM_009141 | 3UTR | GAAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGAUGUAUAUGUAUAUGUAUAUGUGUGUGUAUAU |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 25083871 / GSE57596 |
CLIP-seq Viewer | Link |
186 mmu-miR-466e-3p Target Genes:
Functional analysis: