pre-miRNA Information
pre-miRNA mmu-mir-466e   
Genomic Coordinates chr2: 10479088 - 10479171
Synonyms mmu-mir-466e, Mir466e
Description Mus musculus miR-466e stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-466e-3p
Sequence 51| UAUACAUACACGCACACAUAAGA |73
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Cxcl5   
Synonyms AMCF-II, Cxcl6, ENA-78, GCP-2, LIX, Scyb5, Scyb6
Description chemokine (C-X-C motif) ligand 5
Transcript NM_009141   
Expression
Putative miRNA Targets on Cxcl5
3'UTR of Cxcl5
(miRNA target sites are highlighted)
>Cxcl5|NM_009141|3'UTR
   1 AAAGATTTCTGAGGACTCTGACCCCAGTGAAGATAAGAAGAAAGGGCTGATTCTCTCCACCCACGGATTTTCTTTATGAA
  81 CTCCCTGCTTTGATGAGAAAAGGGAAACCATTGTCCCTGAAGCTTCCTGCTCATCTCACAAGTGTATTTAGCAAAAATAT
 161 TTCCTATTCTTTCCTATCTGTCACACTATCGGAAATTGTGATACTTTAGTGTGTAAGTCAAGAATCATTGGTTGTTAACC
 241 TTTCAAAATGTCTTGAACTGAAGGTGACTATTGCATTGTCTCCGGAATATGTATATGTATATGTATATGTATATGTATAT
 321 GTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGTATATGATGTATATGTATATGTATATGTGTG
 401 TGTATATATATATATATATCCTTTGTAATGTTCATGAGAAGGCAATGCTGTCATACTCTCTCTCATAAAATGGGGACCTC
 481 TTTTACTGTTGTTTCTTGGGTGTGTTAAGAGTGTTCTTACGTCTGAGCCTGGGCTGGCATATAACTTTTATGGTTTTAGA
 561 AAGCTGCTGTGTCATGCAGAAACCTATGTAGAACACAGTTCCTTATTTAGAATTTCTAAATGTGTAAGTTCTAGAAGGAT
 641 CAATGTGTTCTCATCTTATAATTTTAGACATTTGATGTCTTCGTAGTATGGCATAATGTCATGACTTACTCAACAAACTG
 721 TATACTATTTATTAATTATTAACTAGTTTATTAGTATTACTATTTGGGCCACTAACTATATATTTTAGATAGTATATATT
 801 TTAGATAGGTGAAGAAGGTAAGAAGCAAGGAAATTCCTTACCTAGTTTTCATAAAAAACATTTAAAAGTTATTTCTAGGG
 881 AGAGAAACTTTAGCAAATATCTATACTCTTAGAGTTTTGAAAACATACTTAACATGTTTGAATATGGATTGCTGAGATAT
 961 TTAGATGTCTGTAGTGTGTCTTTTAAAGAGTGTGCTTTGTTATAAGGAGATTAATGAATTCATCACATCCATTATTTTAA
1041 GCCTGTACATCTTATAGTGTCTCACTTCTTTATATTACTCTTTTGAGGAAAATAAAGTAGCTCTAGATTCCA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' agaAUACACACGCACAUACAUAu 5'
             ||||| | :|| ||||||| 
Target 5' gaaTATGTATATGTATATGTATa 3'
285 - 307 172.00 -12.20
2
miRNA  3' agaAUACACACGCACAUACAUAu 5'
             ||||| | :|| ||||||| 
Target 5' gtaTATGTATATGTATATGTATa 3'
309 - 331 172.00 -12.20
3
miRNA  3' agaAUACACACGCACAUACAUAu 5'
             ||||| | :|| ||||||| 
Target 5' gtaTATGTATATGTATATGTATa 3'
333 - 355 172.00 -12.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM4751756
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 1
Location of target site NM_009141 | 3UTR | UAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4751760
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 1
Location of target site NM_009141 | 3UTR | UAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_009141 | 3UTR | GAAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGUAUAUGAUGUAUAUGUAUAUGUAUAUGUGUGUGUAUAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
186 mmu-miR-466e-3p Target Genes:
Functional analysis: