miRTarBase - #MIRT588386


—
pre-miRNA Information
pre-miRNA mmu-mir-5114   
Genomic Coordinates chr19: 44303171 - 44303231
Description Mus musculus miR-5114 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-5114
Sequence 1| ACUGGAGACGGAAGCUGCAAGA |22
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Zfp113   
Synonyms 4732456B05Rik, mKIAA4229
Description zinc finger protein 113
Transcript NM_019747   
Expression
Putative miRNA Targets on Zfp113
3'UTR of Zfp113
(miRNA target sites are highlighted)		 
>Zfp113|NM_019747|3'UTR
   1 GAAGCCACATCAGAGACTCCACATGAGAGAGAGCCATAAACTATTTTCTTCATGTCCTGCGTCCACAGAGCTCTTGCCTT
  81 ACCTGTTTTAATAGGACCAGTTTAGGGTCTGTCCCACCTCCAACAATTCACTTAAAGTGGGGCTGACTGGGAAAGCATCC
 161 TGGCACAGGGATCGGCAGGGTTTCCCAGGAACAGAGTCTTAAAAACATTGTGAGGCCAGTAGTAAATTAGGCACTGAGAA
 241 GAGAAGGAAGCTTGGGGACCAGTTACCCCTCTTGGGAAGGGGTTTGCACTAAACCAATTTCCTCATACCAGAAGAGCCTT
 321 TCTTTCCAAGGTGAGGATGGTGCTTGTGTGCATCAGAATTGCCGAGATGTCACAGCTGGAGTCAGTCAGGGAGCACAGAG
 401 GCAGAGAGAATCATAGTCTGGAGTCAGCATGTGCCGAGAAGACAGACAGGTTGTGTTTCTTCTCCTTTTCTTTTTTGTTT
 481 GAGAGACAGATTCTCCCTAGGCATCCCTGGCTATTCTTGACCAGACTGGCTTTGAACTCAGAGAGACCCGCTTGCCTCTG
 561 CCTTCTGAGTATGGGGATTAAAAGCGTATACAACTATTCCAGGCTCCCAGGGGCTGCATCTCAAAAGCACTCTTCCTAAT
 641 CCAGCTTTAATATTGGACAAGGAATATGCTGCTTTGGTACATGATTCCATGAATTCAGGAAACTTGGTGCTGATGGGCTT
 721 ATTTTATTAGGGGGAAACAAAAAAAAAAAAAGACCCCATCAAGGGTTTCTGTAATGACTATCCTTTAGGCTCTTGGAGCA
 801 GTGTACTGTGTTTTCAAGAGCAGTCAGGCAGTACTGTAGGCAGACCCTTGTGACATCACTGACAGAGAATCCCATAGGAG
 881 CAGTAAATAACCTGAGGTAGATCTGAACTGGTAGATTTAACACTGGCCTGACTCCTGTCCTTTTTTAAACTTCCTATTCC
 961 TGTGATGAAAAGTAATTTAATGTCAACCTCTAGTTACTATATGCTCTCAGGGAACTTGCCTGCCCCTCTTGTCCTGCCCT
1041 CTTACATATAACATCTGGCCTCAGCATCTGGACGTCTCCGGATTCCACCTCTTAATTCAGCTACGTCAAAGTACACAGAC
1121 AGTAAAGCCAGAGAGGGGTTGAGGTTTGAGCTTTGCAGGGGCCTCTCCTAGGTTTAGGTGGTGCTCCCTGAGTGCTCCCA
1201 ACCCGACGGATGACTTCCATATTTCCCCACCCTGCCCGTCCTGTAGCAGAAATGAAATGCACTACTAAACAGAATGGTTT
1281 TTCCCAAGGGAGTGCTTTTGTATGGGAGCCACACTTGTGTCAGTTAAAACAGCACAGTGACCGTAAATGGAATCCTAAAG
1361 TGAACTTTGAAACTTTTCTTTGATAAACTTTTTTTGGAGGGGGTGGGGGTGGCACAAACTTGTCACACACAACTCTCTCA
1441 ATCTCTTGTAGACATTGTTGCAGACTATATAAATGTATGTAGCCATCTTCATACCAAATTCAGGGTCTTATGAGCTATAG
1521 ATCATTTTGTCTAAGAATGATGCTGAAAACTAAAAAACTATACTTGGCTCTTGTGTATCAGTGGGTACATTTCTCTGTCT
1601 TGCTTGCTGTCTTTAGCAGGATGTTACAGCCATGGAGAGTGGTAGGCTAGGAATGTTATGATGAGATTAGCATTAAGATG
1681 GCCATGAACTCATTTTTTTATTTTTTGGGCTACTGTGATGGATCTTCTAATATTTGTTTTTAATTTCACAAAATTATGGA
1761 GCCAATCATCCAGGCTCCTTTAGCACTTGTTGCCAGGCCTGATGGTTCAGGCTCTAATCTCAGCTTTGGCGCCTGATAGA
1841 GGAAGATCACAAGTTCAAGGCTTGCCTGGACTACTCTGAGTTCAAGACCAGCTCGTAAGCAGTCAGTTTCCCTGTTGGTC
1921 ATTCTAATTCCTAGAAATTAAGCTGAGCCAAATTCTGCCTCCTTGTCAGTTTTACAGTTAGCTTCTAGTTCTGTCTTTCC
2001 AAGCAACACAACATAATTTTTTATCAAATAAAAATTTAGAGAACATTTTTCTTTTTTTAAAAAAAGATTTATTTATTTAT
2081 TATATGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGATGGAGTCAGATCTCGTTACGGATGGTTGTGAGCCACC
2161 ATGTGGTTGCTGGGATTTGAACTCAGGACCTTTGGAAGAGCAGTTGGGTGCTCTTACCCACTAAGCCATCTCACCAGCCC
2241 CCTAGGGAACATTTTCATTCATAGCAAAATAAGATATGTTATTTCCCCTCTGATGAAGATTAAACCCAGTGTCTCATGCT
2321 GGGAAAGAATTATATACCTCAGCCCCAATGTTTTTTGGTGTGTGTATTCATGCATGCACACACATAGTTGGTTCTCTTGC
2401 TATGTGGGTTTAAGGAATGAAGCTGAGGTCACCAGGCCTGGCAGGGACAAGAGTCTTTGAGCCATATGTCTGATCCATAA
2481 GGGATTTTTGCTTTGTTTTTGTTTTTTGAGGTTTTTTTGGTTTTTTGGTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCT
2561 GGCTGTCCTGGAACTCACTCTATAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTAGG
2641 ATTAAAGGCATGCGCCACCACTGCCCGGCTTGGTTTTTTTGTTTGTTTGTTTTTGTTTGTTTGTTTGTTTGTTTTTTGAC
2721 AAAGTTTCTATGAAGCCCTGGCTGTCCTAGAACTCACTTTGTAGACCAGGCTGGTCTTGAACTCAGAGGTCTGCCTGCCT
2801 CTGCCTCCTGAGTGCTGGGATTGGGTATACCACCACCAACTGGCTGGATTGTTTCTTTTTAGCAAACAATACAAAACAAA
2881 AACAAAAAGAAGGAAAAAAAGTTGTTTTGCTTTTATTTGTGTTTGAGGTGGAACCCTGCTATGTAGCTCAGGCTAGCCTT
2961 GAACTCAATCCTACTGCAACCTTTTCAAATTCTGGAATTACAGGTTAATCCATCATGACCAGTTAAAGATAGTTTTAAAA
3041 AGTTCTCCAAGGAACTATTTATGTAACTTATTTAGACAGGTGGGTTTTTCCCCCTCAATTAAAAGAAAGAAAGAAAGAAA
3121 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAGAAAAGAAAGAAAGAAAAGAAA
3201 AGAAAAGAAAAGAAAAGAAAAGAAAAGAAAGGAAGGAAGAAAGAGGCCCAAAGGCCAGAGATGTAGCTCAGTTTGTAGAG
3281 TATTCGCCGCACTTGCTAAATCCCTAGATTTGAGCTCCAGCACTGTGCAAATTGGGCATGGCTTAATTTAAACACTTAGG
3361 AAGGTCAGGAATTCAAGGTTATCCTCAGTTACATGTGTTTGTAGCTAACCAGAGCTACAAAGCCCTCCGCAATAAACCAT
3441 AGATTTTAGATAGGTGCACTATTTAAAATGTCAGTGATGAATGGGAAATTGTATAGACTATCCACACAAAAACGACTAGA
3521 ATAGTTTGTCCTAAGATAACAGCTGTTTTGCTTTTTCTACAGATTTTATTTTTATTTATCTTTTGAATTATGGGCCCGAG
3601 GAAGCTGTTCAAGGGCACTCGATCCCATGGAGCTGGAGTTACAGCTGTGAACTATCTCACATGGGCTCCCAAATGCCAAA
3681 CTTGGATCTTGCAGAACAGTATTTTAGTAACTGTTCTATGCTACACCGTGGCGAAAGCAACTGTTAAAGAAGAAAGCACT
3761 TAATTGGGGCTTTACTTATAGTTTTAGAGGGTTAGACCATGACCATCATGGCAGGAAACAGACAAGCATAGCACTGGAAC
3841 AGTGCTGAGAGCTTTCTATCTTGATCTCTAGGCAGCAGCCAGGGACAGGGACAGGGGTTGGGGAGTACTGTGACAGAGAG
3921 AGAGGAGAGACAGGTCTTTTTTTTTCCCACATGAGAGAGGTTTATTATTAGGGAGAGAAGAAAAGGAGGGGCAGGGCGAG
4001 TGGAAGGGGAAAGAAGGAAGAGAGAGGAGAGACAGGTCTTGGCATGGGCTTTTGAAACTTCAAAGCCCATTTCCGGTGAT
4081 ACCTCCAACAAGGCAACACCTACTCCTTCCCAAAGAATTCTCTGGGACTAAGCATGCAAGTATATGAGCCTATGGCAGCC
4161 ATTCTCATTCCTAGTCACCTGATGACCACCTGTCCAGCCTAAGAGTGATAATTAGGACTAATTACCCATTCAGGCAGGTT
4241 GCAAACGTGGGAGTTATTCCAAGTGTATTGTGTGATTCCTTCTCATCCCTTCAGCTTCTCAGTCAACATCTGATCCACAC
4321 CTTCTTCCATATTCTCATGACTCCTAAATTGGCCTTGGCCTTTGGTTCCAGCCATACACTCTTCCTACCTACATGTATTA
4401 GTTACTGTATTGTCATGGCCAAATAACTGACATGGATAAGACATCATCTGTGGGTAAGCATTAATCTTGGCTCACAGTTT
4481 CACAGTTTATAGTACACACTTTCCCCTTCTCCAGGTTATCCGTTTATTGTACAAATTGAGTATGACACAATGAATTGACA
4561 TTTCTTCTCCAGATGTGGAACTTAACAGATGAAGTCAGACATTTTAGAATCTTGTTGTTGCTTTTATAGCTGGAGAGTTT
4641 TTAGACTTTAAATAGAAGTATAAGTAAGACCTGCAAAGTATTTCCTCGTCTGAGGTCAATACACAATTCTACCTGTGACA
4721 CTCTAAGAAGTGAATGAAATACAGCGGGGGGTGGGGGGGTTGAGATATGGCTCAGTGACTAAGAGTACTTGCTGTTCTTC
4801 CATAGGACTTGAATTTGGTTACCAGTACCCATTGTTGGGCAGTTCACAATCACCTCTAACTCTAGCTGCAGGGGATCTGA
4881 TGTCCTCTTCTGGCCTCTGTTGGTACCTGCACACACATGGTGTACACATACACAAATAAATTTTAAAAGTTGAAATACCT
4961 TTTAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agAAC-GUCGAAGGCAGAGGUCa 5'
            ||| ||  ||:| ||||||| 
Target 5' aaTTGACA--TTTCTTCTCCAGa 3'
 4553 - 4573 160.00 -15.00
2 
miRNA  3' agaacGUCGAA--GGCAGAGGUCa 5'
               || :||  || ||||||| 
Target 5' gtacaCACTTTCCCCTTCTCCAGg 3'
 4492 - 4515 155.00 -16.20
3 
miRNA  3' agaACGUCGAAGGCAGAGGUCa 5'
             ||:||||   ||||:||| 
Target 5' cacTGTAGCT---GTCTTCAGa 3'
 2093 - 2111 148.00 -18.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM4656410
Method / RBP HITS-CLIP / AGO
Cell line / Condition mouse liver / e185 rep2
Location of target site NM_019747 | 3UTR | ACUGUAGCUGUCUUCAGACACUCCAGAAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE153876
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4751756
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 1
Location of target site NM_019747 | 3UTR | AAAAGAUUUAUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAGCAGUUGGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4751757
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 2
Location of target site NM_019747 | 3UTR | GAUUUAUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAGCA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM4751758
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 3
Location of target site NM_019747 | 3UTR | AAAAGAUUUAUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAGCAGUUGGGUGCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM4751759
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 4
Location of target site NM_019747 | 3UTR | GAUUUAUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAGCAGUUGGGUGCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM4751760
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 1
Location of target site NM_019747 | 3UTR | AUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAGCAGUUGGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 7 for dataset GSM4751761
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 2
Location of target site NM_019747 | 3UTR | GAUUUAUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAGCAGUUGGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 8 for dataset GSM4751762
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 3
Location of target site NM_019747 | 3UTR | AAAGAUUUAUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 9 for dataset GSM4751763
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 4
Location of target site NM_019747 | 3UTR | AGAUUUAUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGAUGGAGUCAGAUCUCGUUACGGAUGGUUGUGAGCCACCAUGUGGUUGCUGGGAUUUGAACUCAGGACCUUUGGAAGAGCAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 10 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_019747 | 3UTR | AUUUAUUUAUUAUAUGUAAGUACACUGUAGCUGUCUUCAGACACUCCAGAAGA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
39 mmu-miR-5114 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577166 Madd MAP-kinase activating death domain 2 12
MIRT580377 Tmem206 transmembrane protein 206 2 4
MIRT580729 Srrm4 serine/arginine repetitive matrix 4 2 2
MIRT581165 Sec23ip Sec23 interacting protein 2 2
MIRT581310 Rnf150 ring finger protein 150 2 4
MIRT581568 Prtg protogenin 2 2
MIRT582280 Nav2 neuron navigator 2 2 2
MIRT582511 March5 membrane-associated ring finger (C3HC4) 5 2 4
MIRT584183 Cpeb3 cytoplasmic polyadenylation element binding protein 3 2 2
MIRT584252 Cmtm6 CKLF-like MARVEL transmembrane domain containing 6 2 2
MIRT584403 Cd63 CD63 antigen 2 2
MIRT586848 Ikbkg inhibitor of kappaB kinase gamma 2 4
MIRT586939 H2-T24 histocompatibility 2, T region locus 24 2 4
MIRT587266 Fam161b family with sequence similarity 161, member B 2 2
MIRT588386 Zfp113 zinc finger protein 113 2 2
MIRT590219 Dync1li2 dynein, cytoplasmic 1 light intermediate chain 2 2 4
MIRT592159 Paxip1 PAX interacting (with transcription-activation domain) protein 1 2 2
MIRT592188 Nanog Nanog homeobox 2 4
MIRT593309 Rmnd5a required for meiotic nuclear division 5 homolog A 2 2
MIRT594051 1200014J11Rik nuclear cap binding subunit 3 2 2
MIRT594495 Cspg4 chondroitin sulfate proteoglycan 4 2 2
MIRT595572 Mtf1 metal response element binding transcription factor 1 2 2
MIRT597122 Svopl SV2 related protein homolog (rat)-like 2 2
MIRT598947 Ggps1 geranylgeranyl diphosphate synthase 1 2 2
MIRT599498 Ccdc78 coiled-coil domain containing 78 2 2
MIRT601067 Cdc14b CDC14 cell division cycle 14B 2 2
MIRT601902 Padi2 peptidyl arginine deiminase, type II 2 2
MIRT602607 Chrna1 cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) 2 2
MIRT602771 Armc2 armadillo repeat containing 2 2 2
MIRT602877 4931428F04Rik RIKEN cDNA 4931428F04 gene 2 2
MIRT602958 Xpo7 exportin 7 2 2
MIRT603304 Slc4a7 solute carrier family 4, sodium bicarbonate cotransporter, member 7 2 2
MIRT603315 Slc35d2 solute carrier family 35, member D2 2 2
MIRT603389 Sgol2 shugoshin 2A 2 2
MIRT605052 Efr3b EFR3 homolog B 2 2
MIRT605054 Arhgef39 Rho guanine nucleotide exchange factor (GEF) 39 1 1
MIRT605412 T2 brachyury 2 2 2
MIRT605550 Ppp1r16b protein phosphatase 1, regulatory (inhibitor) subunit 16B 2 2
MIRT606407 Glrx2 glutaredoxin 2 (thioltransferase) 2 2

Error report submission