miRTarBase - #MIRT588424


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pre-miRNA Information
pre-miRNA mmu-mir-350   
Genomic Coordinates chr1: 176772325 - 176772423
Synonyms Mirn350, mmu-mir-350, Mir350
Description Mus musculus miR-350 stem-loop
Comment Kim et al. cloned 40 new miRNAs from rat E18 primary cortical neurons .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-350-3p
Sequence 61| UUCACAAAGCCCAUACACUUUC |82
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Zbtb6   
Synonyms A830092L04Rik, ZID, Zfp482
Description zinc finger and BTB domain containing 6
Transcript NM_146253   
Expression
Putative miRNA Targets on Zbtb6
3'UTR of Zbtb6
(miRNA target sites are highlighted)		 
>Zbtb6|NM_146253|3'UTR
   1 TCAAATCAATGACTTTTTTAGACACATTTTATAAAAATGCAGCACATGTAGTGTTGCCCCCCACCCAATCTGTATCATTT
  81 ATTTTTGTTCTTACATGTTATTCATTCTGAACTTCTAATAATTCCAAAGTTGGTGGTGTGATTAAGTTTATGAGATTCTA
 161 CATTGTCTCAAACATTATGTATTAGTCCTCTGTTCAGGAGTAATTGCAGATTCTGATTTGTAGTTTTTAGATAAGTGATT
 241 TATTGTTGACCTATATAGAATTTTAAGGAAGTAAGATGGCAAGAAAATAGATTTGAAAGCATAGTGTTGCACATCGCTAT
 321 TAATATTGAAAGCAGATAGGACTAGCAGTTTTTTTTTTTTTTAATTTAACCAGTGTTCTTGATTTATAACCAGAACACCA
 401 AGGTACTGTCTTAAAACTAAAGGAGCACAATTCAAATTCACATGGGGCCAGCAAAATGTCTTTAGAAATACATTACTTAA
 481 ATGTCCAAAAGAATATGTGATTAGACCCTTTTCCTGATTATATATATTGTAGCCTCCCTCAGCCTTGAAGCAGGCTGATC
 561 CAGACTTTGACCTTGCTGCCACTAAGAAGGAGAGGCTAGAGCCTTCTGACCTTGATGGCTCTATTTGCTCTTCTCCAAGA
 641 CTGCTTCCTGCTGAAAGGTCTTTGAGACCGTCCGACCTGTAGATCACCTACTGGCTGGCTCCAGGCACCAAGAATGGGCT
 721 GGCAGGGAATGGGCCTACCCTCTTTATAAGCATGGACTCTCAGTAAACTTCAGAGGCTTTGAACAGAAAGGTTTGTCTTG
 801 GCCTAGTTCTTTTCTTGCCTCTGGTCTCTTTCAGCCCCAGCACGCCTTTCAGGGGAACCCAGTTCTCGTTCAGCAGGCGG
 881 CTACAATATATAACATGGTTGGATTAAGAACTCTGGCCAATGCAAGTATTTTCTTAAATTGACAATTTTCTTAGATTGGC
 961 AAGTTACTTTTCTTAAATAGTCCACTTTCATCAAAGACATTGTTACACTGTTTACTTAGGGTTTCTTTTGGTGTTTGTTT
1041 TGGTTTACATGCACTAAAATTGACCTCTGTGGAATTACATTTCTACGAATTTAGTACATATCTAATTGTCTGCAATTGGG
1121 ACACAGAATAGTTCCACCAGCTTCCAAAATTCCCTTATGCTGCTCTTTTGTAGTCCACCCTTTCTAACTCTAAATTCTAC
1201 TAAGAGTTCTTCATTAATATAATTTGTTCTAATCTGAGGTGATAATAAAACTGGAATCTTATGTTCCCTTTTGATATTTT
1281 TTAACCCCACATAGTGCCATTGAGCTCGATTTATATTTGTGAATATTTGTGTATATTATCAAGTCATTAAGTTTTCATAT
1361 CTCTTATTTTACTTTTGGCATGATTTGTGCAGCTCGGGCTGGCCTCGAGTTTGCCTTGATTCTTGAGCACTGAGATTATG
1441 TCTTTGTTTTGAATTGCCTCTGTATGCATTTTCTTTTCGAGCAGTCATCAGGTGAATAGTTTTAATTTTTAAAAGCTACT
1521 AGAATTAGTTATAATGTCTCATTATATGTATGAATATCTGTACATTCAATTTTTTTGTCCTTACAATGAAGAGCAAAATT
1601 TACTGCCAGGCAAATCACCTTCTCTGACCACAAGAAGTCACTTTCAAACTGTCCTATTTCTCTACATAACTCAGCTCTGT
1681 GTAGAGTTTCTACAGTATTTAAGTTTTATTGACTCTTTTCTATATTTTAACATGTAAAATACTTTGTACTATATAAGAGG
1761 TTTGTGAAAATTATGTGGTATTATATATAATTTTTGTGATACTTTTGTGGGAATAATGCTCTACATTGAATTTTTAACTC
1841 TGGGAGAGACTTTAAAGAATATAAGTCTCAAAGTCACTTCATAAACCAAGTCACTAATTAATCACATTAAGCAGGTCACC
1921 TGTCCTTCAACATTCTTTTTGTTTTTTTTTGTTTTTTTTTGTTTTTTTTTTGTTTTCGAGACAGGGTTTCTCTGTATAGC
2001 CCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGTCCCCTGAGTGCT
2081 GGGATTAAAGGTGTGTGCCACCATGCCCCGCAACTTTTCTTAATTTTATATAAAATAACTTTGATCTTCCTAATTTGTAG
2161 AGTTATTTCTAAGTAAATTTCTACAAATTTATATTCTTCATGTGAACAGAGGAGACATGTCTTTTCAAACACTGCTATTT
2241 GCTATTTGAGTACTCTCTTGGAAACAGAACTTTTTTAAAAGTAAACATTTTGTTCCACTTTATAATGGATTGGTTCAGTG
2321 GCTAATTTCCTTTCGCACATAACTTAAGAATGGCCAGTTATAGTTGTGAGCGTATTCCCCTCAGAGAAACAGTTTGTGAC
2401 TGAAGTGCTTCTCTTATTTTTGCTTCCTGAATTGATTTTTGGAAGGGAGGGTTAGGGATGGGTGGAGTAAACAGTGAGTT
2481 CTTGGGGGAAGTGGTATGTACTATACTGGGTGTTCAGAACATTTTGAATGTGTTTTAAGTTTTATTCGTGCCTTGTAAGA
2561 TTGTTGTAAAATCAAAATTTGTGAGCACTTTGAAAGTTTTGCTTGTGGGGAGGGTGTTTTATAATGTGGTTGTTATTTAT
2641 ATTATTTATTTCCATTACGTGGACAAATTGATCCTAGGAGCTGGGCAAAGTGCACATGCCTATAATTCTAGCACTTGGGA
2721 GATAGAGGCAAGAGATTCAGTTCAGGGCCAGCCTTTCTTATATATAGCTAAGTTTTAGGCCAGCCTCATATACAGGAGAC
2801 ATTGTTTTAAGGAAAACAAAATAAAAAAGTAAAACAAAACAAAAACATTCCTGGGAGAGCATCCGCTTGCATGACATTTC
2881 TGAAAAATTCAGCAAAGTAGAATCTGTAAAGATATGAAGGTATGACAAAGTAAAGTGTAGCATTTATTCATTTTAAATAC
2961 TTTTAGCTGTTTGTTTATGTCAGAAAAAGCAGTATATTTGGGTTGAACCTAGTATGTACTGCATTTAAATGTGTAAAAGA
3041 TCTTGAAAAGGTTGTTACTTTGTTGTAGAGAAAATCCTTTCCTCTTTTCATTGGATGTAATTATGTTTGTATGAAACAAT
3121 AAACCGGAGGTGGGAATGGAGAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cuuucACAUACCCGAAACACUu 5'
               | || | | ||||||| 
Target 5' tactaTATAAGAGGTTTGTGAa 3'
 1747 - 1768 153.00 -9.10
2 
miRNA  3' cuuucACAUACCCGAAACACUu 5'
               | |||   :||||||| 
Target 5' tattaTATATAATTTTTGTGAt 3'
 1779 - 1800 149.00 -6.00
3 
miRNA  3' cuuucACAUACCCGAAACACUu 5'
               | |||    ||||||| 
Target 5' ctcgaTTTAT---ATTTGTGAa 3'
 1305 - 1323 142.00 -6.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' cuuucACAUACCCGAAACACUu 5'
               | |||    ||||||| 
Target 5' ---gaUUUAU---AUUUGUGAa 3'
 		1 - 16
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
27 mmu-miR-350-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT435011 Lars2 leucyl-tRNA synthetase, mitochondrial 1 2
MIRT577176 Tet1 tet methylcytosine dioxygenase 1 1 5
MIRT577951 Pole4 polymerase (DNA-directed), epsilon 4 (p12 subunit) 1 1
MIRT579173 Cd70 CD70 antigen 1 1
MIRT580234 Tsga10 testis specific 10 1 1
MIRT581630 Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit 1 1
MIRT584029 D230025D16Rik RIKEN cDNA D230025D16 gene 1 1
MIRT588424 Zbtb6 zinc finger and BTB domain containing 6 1 1
MIRT588528 Usp45 ubiquitin specific petidase 45 1 2
MIRT588917 Skor1 SKI family transcriptional corepressor 1 1 1
MIRT589679 Larp1 La ribonucleoprotein domain family, member 1 1 2
MIRT590168 Elp4 elongator acetyltransferase complex subunit 4 1 1
MIRT593065 Ehf ets homologous factor 1 1
MIRT593183 BC068281 WD repeat and coiled coil containing 1 4
MIRT595160 Cbfa2t3 core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) 1 1
MIRT596758 Utp23 UTP23 small subunit processome component 1 1
MIRT596783 Ubxn2a UBX domain protein 2A 1 1
MIRT597289 Slc1a7 solute carrier family 1 (glutamate transporter), member 7 1 1
MIRT597494 Rbm41 RNA binding motif protein 41 1 1
MIRT600204 Ube2j2 ubiquitin-conjugating enzyme E2J 2 1 1
MIRT603448 Ric3 RIC3 acetylcholine receptor chaperone 1 1
MIRT604253 Cercam cerebral endothelial cell adhesion molecule 1 1
MIRT604915 Iqcj IQ motif containing J 1 1
MIRT605131 Cnnm3 cyclin M3 1 1
MIRT605214 Arnt aryl hydrocarbon receptor nuclear translocator 1 1
MIRT605697 Ifnar1 interferon (alpha and beta) receptor 1 1 1
MIRT606256 Polr1e polymerase (RNA) I polypeptide E 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-350 Hesperidin NULL 10621 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-350 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 down-regulated
miR-350 Tamoxifen approved 2733526 Microarray rat liver 17343880 2007 down-regulated
miR-350 Ursodeoxycholic acid (UDCA) approved 31401 Microarray rat Liver 20689055 2010 down-regulated

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