miRTarBase - #MIRT588629


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pre-miRNA Information
pre-miRNA mmu-mir-384   
Genomic Coordinates chrX: 105344282 - 105344369
Synonyms Mirn384, mmu-mir-384, Mir384
Description Mus musculus miR-384 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-384-5p
Sequence 16| UGUAAACAAUUCCUAGGCAAUGU |38
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BZV7T3 miR-384-5p Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Serum Real-time reverse transcription PCR
Gene Information
Gene Symbol Tnrc6b   
Synonyms 2700090M07Rik, A730065C02Rik, AI848765, D230019K20Rik
Description trinucleotide repeat containing 6b
Transcript NM_144812   
Other Transcripts NM_177124   
Expression
Putative miRNA Targets on Tnrc6b
3'UTR of Tnrc6b
(miRNA target sites are highlighted)		 
>Tnrc6b|NM_144812|3'UTR
   1 ACTTAAACTTTCAACTCTGACCTCGTGACCTTTTTTGGAACAGCAGCAGCACTAACTTGACCTTTTCGTTTTTTTTTTCA
  81 ACTTGCAATAAATACATTTTTAAAAGGAAAAAAGAAAACGGAGAGAGAAAAAAGGTGGGTCATTGACAGACTGTCTGAGC
 161 ACATAGTTGCCTCCCTTAAAACTTCAGTTTTTTCGTTTGGAATATGAATCCAAAAAGAGAACATATCACTCTTGAAATAC
 241 TTGAATCATGAACGCCAACCTAGAAAGACAATGTGAAGCAAGTACACATACCATTTAAATTTAAACACAAAAAAATTAAA
 321 AAAATTAGTTTCTAGTTTTTCACTTTTTTCATGTTATATGGAAGTTGTTGCTAAGAAACATATATACTGAAAAAATAGCT
 401 TTTTAACTTTGTTTGCACTGGGTGTGTTTCCGTCCTTAGGTCTACCATGTTTGGGTGGGAGGGAAATTCAAAAGAATTGT
 481 TGGGGGAGGGGGGAGGGAAGACTTGACGGAGCCTCACTTTAAAAACAAAAACAAAAAAACTTAAAAAAAAAAAAAAGGTA
 561 AAATTTTGTGATTGGCTGGTTGATAAATACCAGTGTGTTCTGGCACATGTAACTGCCCAGGCATGCTCGTTCTGGTATGT
 641 GTGTGCACGTGTGTTCATGTGCATTTGCGTGCATCCTCCTCTCTGTCTCTCTTCGTGTGCCTGCGTCCTGCCCTCCTCGT
 721 CCTGCCTCGCCCCGCCCCACCCCCACCCTGATTTCTCAGAAAGCTGCATTGCTGACAGTTCCAAAGGCTGGCTGGCCGGC
 801 CATCCGCTTTTCATTATTTCTGACTATGAGAACACAGGAGCACAGGGAGCCGCTGCAACTCAAGCCACTTCTTCTGGCTG
 881 CCTCGAGATGAAGGGTGCTTTTCAGTGACAGAGCAGCAGCCAGAATGCGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGAG
 961 GTCCCCAGCTTCTGTCGACCCAGCTGCAGAGCAGAGCACTGCCAAGGCCAGAGGTGTGACAGAAGGCCCCTCCCTCCAAG
1041 AAGCTGCTGCTCACCTCTGTGCTGTGAAGTAAGCAGCTGCCTGCCCGGCAGCAGGAGTCTAAGGACTGAGCTGGCCAGTT
1121 GGCTACTTTGAGTGAGAACAGACGATTCAATAATTTTTAAATCGTGTGATGTTTTAAAAGCTGAAAAACAGTTCCCACTT
1201 TGCTGTGCTCGTCGGGTGTGAGAATTTTTTGAAAGGATTCAGGTAATGCAAATTCAATTACTTCTATCAGTTCTCATTAT
1281 CATGTGAGGAAGACAGTAAGTTATGAGCAGCCTCCTGGAAGCATCGCAGCGCCTCCCTCTCTGCTCCCCTCTCTCTCTCT
1361 CTCGCTGAGCATTACCTGTGCCAGGCCGTGGCGTGTTACAGCACCAGGCCATCACTGACAGAAACGTTTACTTAACGAAT
1441 GTTCTTAAACCAGTGTGTACTTCAAGTGTCTCCCTTTGTTTCTCTGTGTTGTTTCCTGTGTATGTGGTTTTGCTTAGGCA
1521 GAAATAACTTAGCAAAGTATTGCACCTTGTTTTGGTTTTGACCTCTTTTTTTTTTTTTTCTTTCCTTGTAATGTTGGTTT
1601 TTTTATTTTGGTATTTAAAGACTTTTTTAAAAAAACATCAATGTAGTAGTTCTCTGGGCCGAACAGGAGGCAATGTTTAA
1681 TCCACAGTTATCACCAATACGTATGTACTATGTTGGAATCAAAATATATCAAATTTCTTATTGTGAAGAGAGTGAAAGTA
1761 AACCCGAAGGGGATCCTCACGTGCTAAATAGATGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGTGATGTGTGCG
1841 GGGAACATGCTAGCACTTGTGTGATATCCCAGTCTGCATCGTACTGTCTGTTCAACATGAAGTGCCGACATTCTGTACCA
1921 GCAATTACCTGCCCATTCCAACCCCTGACGGGAGCAGACCTCTGCTGTCCTCTGTGACTTGCTGCTATTGAGGACAAGGG
2001 AGTTTTTCTTTCTTTAGCATCTTCAGTGAAGGATACAACAATGCCTAATGTATTTAACTTTCAGCTTGTGTCTTTGGTGT
2081 AGGTGGGTAGAGGGGTATGGATAGGAAAAAGCAAACAGACAAACAAAAAACCCAAAATTGCATGTAAGTGGCTTTGTAAG
2161 ACAGTGAGTGGGAAATTCTGTAGTCGTCTTGGCTTACCTTGTTGGGTATAAACCTAGCTTCAGAGGAGGCTGACGGGGCA
2241 CCATTCCCATGAGCACCTGTGCTTAACAATCTGGGCGCCCTTGGCAGGTGGGTAGGCAAATGAGCGTGCAGTTCTGGTTT
2321 GTTCCTGCAACACCTTGTTCTCATGGACTCTGCCTTCCTCCTGCTCTCTGGTTTCCATCACCACCACCCCCTCTCACCTC
2401 TGCCCTGCAAAATGTCTCCTTCCAGCCCCACTGACTCTCAGTGTTGGAGCAGAGCTCTGTCGCAGGTGATGACGTCCAGG
2481 TGAAGCGCCGCTTATATTCAGACATCTGCAGACTGAAGGCTGAACTTTAGTGTCCCCAGACTCCTGGGAGCCTGTGGGGA
2561 CTGTCCACTGCCGCTCTTGGTTCATGGCCCTGCCTATCCTCTCCTCTGCCCCTGCTCCTCTTTCTGCCTGTTGGGTCCTT
2641 GACCTGCACTATGGCTTAGTTGAGTTCCTTACGATACTACGGTGAAAGCCGGGTGCTAGAGCCCCTCTTGTTTACAAGAC
2721 ATAAATGAGGGATTTGAAATATGTAAAAATAAGCATGAGAAGCTAAAATGTTCTTCCATCATAAGCTTAAAGGGAAAAAA
2801 ACTAAAAACTTAAAAAAAAAAAAAAGACCAAAAAGTGCGTGTGTGTGTGTGTGTGTGTGTGTATATATATATATATGTGT
2881 GTGTATATATATATACATATATATATATATATGTATGTACATATATATATGCACATACACACATACATATAAATATATAT
2961 TTTAGATGAAGACTAACTCTGGGAGCATTTAACAGTGTTTTTGTTTTTTATTTTTAACATTATTTTCCTGCTTTAAAATT
3041 TGCAAGCATTCTCAGGAATTCTAGGGTAGGAAGCCAGTTTTTTGAAGATGGTTTATTTAATTGTATCTTATCCTAGATAG
3121 ACAGCTGTTTCTTTCCTGTTAGTAAACTTTTACAAGTTCCTGAAACTTCAAAAAGTTTAAACGATTTTTACTTATAAAAA
3201 TGTAAAAAGAAAAGTCTCCAAACGTTATTATCATACAGGGGATCACACATTTTAAAATACATAGAAATATTTTTTAAAAA
3281 TTACCAATTTAGCAGCAACAGGTAAATTCATTATCTTAACAACTGTGGGAGAAGGCTCAGCCATTCTGAGTGTTGTCACC
3361 GCCTTGCTTGACATTGTGCATGGCTTACTGGGGGGGTGACTGTTGAGCTCGTAGGAGTGACACCACATTGTTCCTTCTGT
3441 ATGAGGCTGGACTGACGTCTCCCCGTCATGTTTCTGTTCTCCAGTGCAGCCCTTCTTCTCCCTGGCTCTCCAGCCCCATC
3521 AACAAAAGCAAGGAATAAAGAAAGAAACAGTAGCTTAAGCAAAAGGATCGGCTCTAAACTGTGGCAGTGCGGTTGCTGGA
3601 TTGCTGAGAATCTCTGTAAAGAGTTAGGAAATATAGGTTAATAATTTTTTAAACGAATTTTAAATATGAGCATTACTAAA
3681 GGAAAATTACCTATGGACTGTTCTGTTGGTGAAAAGTGTGTGTTTCGCCTCCCATCTGTTACAGGAGGAGGAAACAGAAG
3761 AGTCTAATAAGGAACAAGTGGTTTTACTTTTCATTTTTTATTTAGCTCCCAGCTACTGTTTGCTTGTTTTAAATGCTGTT
3841 TTGAAGATCTGCTGCTTACTCCCCCCATCCTGGGAGGCAGAGATGTGGAGTGTTTCCTTTTGATTTGGGTGATGTTGAAT
3921 TCTGCTTTTGTATTCCCTCCTGGGGCCAGAGGTCTTGGCCACAGCTTCTTTCAAACTTTAAGCTTCCCAGTGGGAAAGAA
4001 ACTCCAGCTCCACAGGTAAAGTGAATGGGTGGGGTTGTTGCCTGCATTCTCTAAAACCGCTTTAGTGCCTTCGTACATAC
4081 ACCGACATCCTAAGCAGGGGATGCGTGTGCGTGTGCATGTGGTCCAGTCACTCCACGGTCCACGGTCGGTATGATGCTTG
4161 CCTGTAAAGGAAATTGGAGTGGTTCTGGACAGGCCCCAAAGGCAGTGGGCTAAATCAAAATAAACATTGAGGAAGTTAAA
4241 TACATTGTTTTGTCTCGATAATGCGGGATGCCAAGTTCCTTCCTATGTTGTGTGCCCTCCATAGGTGCTGACCTCTCCAT
4321 GTGTAGAAGGGTGACAGTTAACAGCCATGGGGAAAGTCTTTTTGTGCCGGGCCAGTGGAGGAAGGCTCCTGCTTCTCAGC
4401 TCCTGTTTCTGTCTTCTCTCTGCCTCTCCCCTTCACCCATTTGTCATCTTGCTGGAAGCTTTTGTGATTGATAGGCAATG
4481 ACCTAAGAAGAGGATGTGGTGAGGGGTACCCTGTCTACTAAGACATTAGGCGACTTTGTCCTACATGGAAAAGTTCAGTA
4561 AGCATATATTTCCAGCCGATCCTGGCATATATACAGAGGCTTGCTAGTCACCATTATATAGATTTGTTTTATACACATTA
4641 TAAGCATGTAAGTGTGAGCTTTCTGTTTTATTCTAGTTTTTTTTTTCTAACTCTTGATTAGTAGTAGGTAGAGGTATATA
4721 TGTCTCGTGAGCATCAAGGCTTGTTGTCTGACCCCCATGGATAGCCAAAAGAAAAGCACAGGTGTAGGAATGAGTCTTCT
4801 GTCACTGGGGAGCCTCTGCTGCCGATGCGACCCAGTGCCATGACCCCATTTAAGGGTTGAAAAAAACAGGTCTCTGACAC
4881 CCTCTTTAAAACCAACAGAAGAGAAAGGACAGTCCCGTACAGCATCTCACCTCTCAGCCCCTAGTTCTTGAACTTATTTT
4961 CCATTTCCAGCATTTATAGGACTAAACTTACCCATGCAGCAGTGCTGGTCCACAGAACAGCAGCTCAAAACCATACCACC
5041 CCCCTCAGCATCCCCATGAAGCACCCCCTGTGCTTTGCCATTCAACCAGTTACTTTGTATTGCAGTGGCTTGTGTTCTGA
5121 GAAATGTCCCACTTCTGTTGTGAATGTGGCATTGGGGATTGAGTCAAAATTCAGTGTGATGTGGAAGATAATGTTTAAGG
5201 GGGAGAAAGAAAGAATTTGGAAGGGCTTTACTAGTGTGCCTACCAAAAACTGAAAGTTCACTTACTGCAGACACTTCACT
5281 CCCTGCTTGGAGTTCATTGTGCTGGGGTTGAAACCCAGGACTTTGCCCATGGCCGTGAGCACTCTCTGTGCCTGAGCCAC
5361 ACAGTTCTTCCAGCCCTATGCCATTGCTGTCCGTGCCCCCTTTTTCTTGTAAGGGAAATGGTTTTGTAGGATTAACGGCC
5441 AGGTTCTACTTTGACCTCAGATGCTGTGTACTTTTCAATTACTTTCTCATCTTCTGCCATTTTGACTCGCGTCTCTGTGT
5521 CCCGCTTCCCTCTCCAGTATCAGCAGGATGTTCTCAGCAGATGTGGCTGAGATAGCCATCTCTAAGGTTGAGGCACAACT
5601 GAGTGGTCCCTTTCCTCCCTGCCCTCAGAGGTACAATTCAGCATGTACTGCTCTGACATGTTACTTTGAGTTAGTGTTTT
5681 GTTTTGTTTTGTTTTGTTTTGTTTATTTTAACTATAGGAGAAGAGATACATTTTAGCTGGTTTCAAACAAAAACCCCAAA
5761 CTTTATTCTCCATGTTTTTGCCAAGTTTTGTCACAGTTTTGAGTCCATGAATTAGCTACATGTCTTCATATCACTGCAAA
5841 GAGAAACCTTGTTCTGTGGTCATGCTTATCTTCTCTGAGTGCAGCTAGGACTATTCTAGCATGTGTGTAAGTGAACTGTC
5921 CACCCTTAGACTCTGAGGACCACACAGAGCCCCTTTGCTCTTTACCTACAAGGACAGGGCTGAAAGAAAAACAAAACAAC
6001 CCCAGAATATCCCCAGAGTTTGTTTCACCCTATAAGAATGGTCTATAGGCTTGCATCCAGTGCCAAAGGTACGTTGTATC
6081 AGAGATGGCTCCAGTTGTCAGCAATAGTATTCTTATTCTTGACCTTAACTTTGACAAAGGGGGTAGCCATACTGCAGTCT
6161 TTAGATTTGAAATCATTGTCAACTCTAGGAATCTAAAGACTGAGTATTGGACTAGACTTGGTGGCACATGCCTTTTATCC
6241 CAGCACTTGAGAGGCAGAAGCAAGTGGACCTCTGTGAGTTCAAGGCCAGCCTGTTCTACATAGCAAGTTTCAGAGAGACC
6321 CTGTCTCATAAAGCAAAATCTGAATACCAATCCTCTCTAAACGTCCCACAACATCGCCCCCACTTCCCACTGGAGGCCAC
6401 AAGGAGGACATTAACAAGATACAAGATGGTGAAAGAGGAGTACTAGTCAGAGTGGCACTCTGCCTCCCATGTGTGTGGAC
6481 CCTGACCAGGTCCTGACAGATGGCTAGTATTTTCATCTTTTGAATGATTTTTTAAATAGTAACTGAGTGGCAGAGAATGC
6561 CAATGTTTGCCTCTGGGATTTGAAATGGGCCATACCAGGAACGGGGGTACTATCTGAACCAGAAGGCTCTTCAGAGCTCA
6641 GCACTGTTCCTCAAGGTGACAGACTTACCTTTCCACCAGCGGGGAAAGGCCAGATATCCTTTCTTAAGCCAGAAGTGTTC
6721 TATTTTAAATAAGAAAGAAATAGTATTTCCCTAGGGCTCTACCTGCTTCCCTTAGTGCTTAGGTTTCACAGTGTGAGTCA
6801 GCGCTGGAAACAAACTCTCCATTACAGTTGACCCTTTTCCCAGTCAGTATCCGAAAGTTGAGATGACCTCTTCTAACAGG
6881 GAAATCCCTTCTTAGATCTCTCGCCCTTCTCCCTGGCTTCCTGGACCTTCTGGTGGGGCATGAAGTTTTTCTTGGGCCAC
6961 TCGTGGAGGTTGTAAAAGGGACTTGAGGCAGATGGAGCAGCCTTACCTTCTGTGCCTGGCCTTACAGCAGGAAAAGGTGG
7041 TGGAGTGCTGAATGTGTATGCCAAAACCAAACCAACCCTTGGGGGATCTTTGAAGAGGTTACATACCTTGTAAAGGTAGG
7121 ATGGTCGCTCACCAAGTGATGGCTTTTAGCATGAAACTTCTTTTTGCCAGAAGTTTGGGGTTATGTTACAATGCTTGTGT
7201 TTAAAACACTGCCCCCCTTTAATTCTTTCCAAGGAATCTCTTTAAGTGTAGTACTTCTGAATGTGCCTTTGTCAGCCCAG
7281 AGACCAACTGTGGTCTGTCAGAGGTTCTCCTGGCAGCCCCAGGGGCAGCTGTTGGATTTCCATGAGCACCCATTTTGCAC
7361 AGTGTGAGTTGTATGGAAGAGATTCTGGGTCTTGGACCAGGACCTTATAAGCAAGGCCAGGGCCCAGCGGATTCCACTGT
7441 AGTCAGCTGTCGACATCTGCATTCCTTAGTGTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTGTGTGTGTG
7521 TGTGTGTGTGTGTGTTGTAAGCACTAGAGATTTGGGATTGGGAAAGGGTGATTTTTTTTGTGACCCGTTCATTCTTGTAA
7601 AGAAGGCATTTCTGGCTTTGTGCTGCAGAAGGTGCTTTTTTTTTTTTAGTTTAATTTTATTTTATTTTTACCCTCTCTGC
7681 CTTAGAAGGTCATGATTTTCCCAAATGCAAGTCACATCATGTTGCTATTTCTGTGAACAGGGTATTTATTGCACATAAAG
7761 GAAAGGTTTTGAAAGAATTGAGACCGGTGTTTAAAAAAGAAGAAAACCTCTGAAATCACGTTGTAGTTTTTGAGAACTGT
7841 TGTAGTTAAACTTGGAAGTCTATGGTCTTTTCATGTTTCATGTTTGTTTTCAACGACCCCACCTCCACTCCTATTTCCAG
7921 TTTCTAAAGAAGCATCTTAGTGGATGCCCAGGGGCCTGTCCACCATCCTCAGTGCTCCCCTCCCCCAGGAAAGTGCTCTC
8001 TAGATTCCTTCAGCTTCATTCAGGAAGACACTCACTCCTCTTGTTGCCTTGGGATGTATTATTATGCAAATGTGAATTTA
8081 ACTAGAGGTTGTTGGATTTGTTTTTATGTTTTGTTTTGTTTTTTTTTAAAAAAAAAAAGTCTAAATGTGGTCATCCTTAC
8161 TCTATTTTATATCCTGAATTTGTAACTATGCATTTTCTGAAAAGGGGGAAGGCAGGGAAAGAAGCCGATTTGCATCCTTA
8241 TGTACTCCAGTTTCTGTGTCCATGGAATCCCCATGTCTGGGAAGGGAAGAAGAGGAGAGGAGAAGGCTCGCTAGGTCAGC
8321 ATAGTGAGGGCGAGAGGGACCTCCCAGACCCCATCCTCTGGAAGGTCTGATTGCCAAGCAAATTTGGGGGAGAGGGGGGT
8401 CTGCCATGTCTGTCCATCATTACGGTGCAGGATTCTTGATACTGGCCATGCAGTGTTAACCCAGGGCAGCCATGGAAGCT
8481 CACAGGCTCCAGTATTCTCTGAACTCTGAACCCTTTCAAGCTCAGGAAACCATGCCACACTCTGCTGACCTGAGAGTTCA
8561 TCTTCTTCTTTTACGTTCTTTGTTTTCAGTCATTGTGTAGGGTACCACTCCTAATTATGAGCCGAGAAAGAGGGCTGTTC
8641 CCTTCCATGTCTGCTCCCTGACCTCTGTTTCCCTCACCTGTACTGAGCTGGTGGCACAGCGAGTGAGTTGTGGGACACTG
8721 GAATGCTTTCATACTTTCTCTTTGTTGTTACAACAATTCCTGTGTAGAGTTGGACTATGCTAAGTCATAGCGCCTGCATT
8801 GCTCTGGTCTAGGCATTATTACAGCACGTAGTATAGAAAGCCAGAGATGTTCAGCCTGTCTTCAGTGTTTCCCACACAGC
8881 AATCTCTGTTATGTTCACAGTACTGTAATGAGTGACAGACGTGATCACAGTCTCCTTGTGTCTGTGCAGGGCTGCACAGG
8961 CATGATTGTATCAGGGTTCCAATCTGTTCGATTGCCTTTAAACAAAAGCAAAACAACAAGAAAATCCTGATGTTTCAAGT
9041 GTCTGACACAAAATGATACCTCAAGGTATATCTCCCCTGCAATGTAAACCCTTCACTCCTCCTTTCTTGACATCAGTGAG
9121 CTATTCTTTATACAATAAGGATGTGTAAATAACCAGATAAGTCTTTGAAACATTTTCTTTGCTGTGAAAATAACCCTAAA
9201 ATATGATATTTGCATGTTAAGCCGTCAGAAGTTTCAGATCTGAATTCTTGTTGATGAAGAAAAAGCAAAGCAGACGGGTG
9281 GGATGGCTTTGGGTTTGTGGTTTGCCTATTCTCTCCTGGTGTCCCTCCCCCGCCACAGCACAGGGCAGCAGACCAGTGAC
9361 CACACCCTGCCCTAACTAAATGCCATCTCTTCCACCACCAAGGCCCCAGGGTGGGCAATATCTGAATTTCTCTCTTCCAT
9441 ATATGATCCATACCCTCCAGGGACTTCAAGAAGTTCTTTGCCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
9521 GTGTGTGTTTACATGTATGCACTTCTTGCTCTTTAAGTGTGGTCCTTTAATCCCCAGTGGAGTTTAGTCCCTGATTTTTT
9601 AGTCTGTCCCGTAGCCTTTTTAGCAATGTTCTGGAAATGTATTTCTTACTTAGAAGGAAATTGAGCTTAGAGCTGACATC
9681 CTTAAAAATCCTTGTTGGCACATGCTGCCTGTGAGGGTGCTGACAGCCGCTGCCCTCCTAGACCGTGGGCTCCCTTGAAC
9761 AAGAGTAGAAGATTCATTTTATCTCATTTTAACTCAAATCTATATCTGTTAAGAAGCTATAGTCAAGAGTGCTTTCCTTG
9841 ATCTGTTTAATGACGACTTCCTTTGAGGGAGGAATCTGGTTGAAAGTGATGGACATCCCTTGCCTATCTTCTGCGTGGCA
9921 CTGTTCCCTTGGATTTGACTTCTGTAGTTTGTCTGGAGGTCCTTGCTTATCAGTGTGCTTTCTCCTATCGACGGGGTTCT
10001 GCCTGCCCTTGTTTCCCTCTCTGATGTTCCCAAAGGGCTTTGCTGATGTCTGCACCTCTTTCTTGGGGCATTCCCCTACT
10081 ACACCCTTTGCACACACCAGTGACCAAAGTGTATGCAGTGGTCTCAACACATGACATCAAATGGTGCACGCACCTCTAGT
10161 GTGCTCACAGATGTGCACAAGATCTGCACCCTTGGCCTTAACTTCAGCACGACTTAAGGTGCAGGAACATTTTTAACAGG
10241 TTTAAAAATAACCTTTTCTGGGAGGGAAAATCAAGCAGATCCAACCGCTCCCCACTATCTGCTTGCAACTCATTCTGCAT
10321 CCAGCCCAGCCCAGCCCTGTTTTGCGTAACCAAAACCAACAACAGAAAGCAACCTAAGGAGAGGTCTCTGGACTGTTTTA
10401 TCTTTCTCCCTTTCGATGGAGATACAAATGCTGCTTTGGGTTCCATAATCCTAGGGGGCTATGTTTACATAGTGAAACTA
10481 ATTCTACCAAATCAGGAAACAGTGAGTAGTGAGAGACCCTTCAGTAAGAAATCACTGAGGCTGAACCAGAGGTTTAAAGA
10561 CCTAGGGATGGGACAGAGGAGGAGAGAGATCCTGTTTAACTCACTGCAACACCAGGGTCACTCCCTCCTCTCTGCTCTCC
10641 CTGGCCCTCTTTGCCCCTTTAATCAAAAGGTTCAGAGAAAGACACCAGAAAGAGGCTGAAGTTTTTAAGAGGCAGCTTTC
10721 CAACTTTGCACACAAATGGAAGGTACCATGAAAATCCTCTCATCCGAAACACTCGGCAGAAACAACCTGTCAAATCAAAA
10801 GGACTAAACTGCTGCCCACGTTGATGCGCTCTGCGAGTTACCTATAAGTGTTTTGTTTTCTTATACTTGAAAACACTTTT
10881 ACAATATTATGTTGGTGGCTTTCTTTTCTCTCTTCTGTTGGGCAATTGAAACTGCAGATGACAAATGTTAAGGCTGTTTG
10961 AGGGGAGAAAAGGAAGGCTGTGGCATTGTCGTTTTAACATAGAGGTTCAGTCAGACTCCCATAGGGTAACATTGTTCATC
11041 TTACTGGTTTAACATGGACACAGAAACTAGGCACTTTGGTGGTTCACTTGCACGGCAGGCCGACCCCCTTTCTATATTTT
11121 ATTTTACTTTTTTAGTATAGTGGTACTTAAAATCACTGGTTCACTTAAAAAACAAATGTTAAACTCTACTAATGTACAAA
11201 TAAGCTGAAAAGTTGCATTTTATGTGTATTTTTGCCATAGCAGGTACTGTATTTCTCATGCTGGATTTCAAAAAAAAATT
11281 ATATATATATATATATATATATCAAAAACAAACAAAACTGAAGGGTGGTGTTTTCTCAGATTGTGACAGGTTGATTGGTA
11361 AGGATTAAAGTCGTAGTCATTTCATTAAAACTGAGAGATGATGTAATGCATATATAAGAGTTTTCTGAAGGGTTTTTTGG
11441 GCTTTTAAACAGCTTATTTTGTTTTTTTTTTTAGTTTTTATTTTATTTTATTTTGGAAAGATATGATTGTATTATGTGCA
11521 ACTCAGTTGCTTACATTATAACTACAAAATATTTTTGAGTTCCTGGAAAAAAAAGAAAAAAGACTAATAAATGTGTTTGG
11601 CTGCTAAGCA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uguaacGGAUCCUU--A-ACAAAUGu 5'
                ||||||::  | ||||||| 
Target 5' cataatCCTAGGGGGCTATGTTTACa 3'
 10444 - 10469 159.00 -19.30
2 
miRNA  3' uguaACGGAUCCUUA-ACAAAUGu 5'
              ||: |: | :| ||||||| 
Target 5' tgtgTGTGTGTGTGTGTGTTTACa 3'
 9510 - 9533 151.00 -10.10
3 
miRNA  3' uguaACGGAUCCUU------AACAAAUGu 5'
              || ||| ||:      |||||||| 
Target 5' cgggTG-CTA-GAGCCCCTCTTGTTTACa 3'
 2690 - 2716 147.00 -12.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Brain (Mouse neocortex)
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_BrainB_130_50. RNA binding protein: AGO. Condition:Brain B 2A8 P13 130 KD HITS-CLIP data was present in Chi_BrainC_130_50. RNA binding protein: AGO. Condition:Brain C 2A8 P13 130 KD ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Liver
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in ERR266281. RNA binding protein: AGO2. Condition:A_Liver partial hapatectomy 48h HITS-CLIP data was present in ERR266286. RNA binding protein: AGO2. Condition:A_Liver partial hapatectomy 1h HITS-CLIP data was present in ERR266287. RNA binding protein: AGO2. Condition:B_Liver partial hapatectomy 36h HITS-CLIP data was present in ERR266292. RNA binding protein: AGO2. Condition:B_Liver partial hapatectomy 48h HITS-CLIP data was present in ERR266293. RNA binding protein: AGO2. Condition:A_Liver partial hapatectomy 36h HITS-CLIP data was present in ERR266295. RNA binding protein: AGO2. Condition:B_Liver partial hapatectomy 1h HITS-CLIP data was present in ERR266298. RNA binding protein: AGO2. Condition:A_Untreated HITS-CLIP data was present in ERR266300. RNA binding protein: AGO2. Condition:B_Untreated ...

- Schug J; McKenna LB; Walton G; Hand N; et al., 2013, BMC genomics.

Article - Schug J; McKenna LB; Walton G; Hand N; et al.
- BMC genomics, 2013
BACKGROUND: Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs. RESULTS: miRNA recruitment to the RISC is highly dynamic, changing more than five-fold for several miRNAs. miRNA recruitment to the RISC did not correlate with changes in overall miRNA expression for these dynamically recruited miRNAs, emphasizing the necessity to determine miRNA recruitment to the RISC in order to fully assess the impact of miRNA regulation. We incorporated RNA-seq quantification of total mRNA to identify expression-weighted Ago footprints, and developed a microRNA regulatory element (MRE) prediction algorithm that represents a greater than 20-fold refinement over computational methods alone. These high confidence MREs were used to generate candidate 'competing endogenous RNA' (ceRNA) networks. CONCLUSION: HITS-CLIP analysis provide novel insights into global miRNA:mRNA relationships in the regenerating liver.
LinkOut: [PMID: 23597149]
Experimental Support 3 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM4751757
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 2
Location of target site NM_177124 | 3UTR | GUGUGUGUGUGUGUGUGUGUGUUUACAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset Chi_BrainB_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition Brain (Mouse neocortex) / Brain B 2A8 P13 130 KD
Location of target site NM_177124 | 3UTR | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACAUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset Chi_BrainC_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition Brain (Mouse neocortex) / Brain C 2A8 P13 130 KD
Location of target site NM_144812 | 3UTR | UUUGCCGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset ERR266281
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / A_Liver partial hapatectomy 48h
Location of target site NM_177124 | 3UTR | AGAAGUUCUUUGCCGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset ERR266286
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / A_Liver partial hapatectomy 1h
Location of target site NM_177124 | 3UTR | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACAUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset ERR266287
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / B_Liver partial hapatectomy 36h
Location of target site NM_177124 | 3UTR | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACAUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 7 for dataset ERR266292
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / B_Liver partial hapatectomy 48h
Location of target site NM_144812 | 3UTR | AGAAGUUCUUUGCCGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 8 for dataset ERR266293
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / A_Liver partial hapatectomy 36h
Location of target site NM_144812 | 3UTR | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACAUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 9 for dataset ERR266295
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / B_Liver partial hapatectomy 1h
Location of target site NM_177124 | 3UTR | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACAUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 10 for dataset ERR266298
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / A_Untreated
Location of target site NM_177124 | 3UTR | GCCGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 11 for dataset ERR266300
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Liver / B_Untreated
Location of target site NM_177124 | 3UTR | AGAAGUUCUUUGCCGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23597149 / E-MTAB-1612
CLIP-seq Viewer Link
CLIP-seq Support 12 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_144812 | 3UTR | AAGUUCUUUGCCGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUUUACAUGUAUGCA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
37 mmu-miR-384-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577998 Pgr progesterone receptor 2 2
MIRT578191 Narg2 interactor of little elongation complex ELL subunit 2 2 2
MIRT578276 Mesdc2 mesoderm development LRP chaperone 2 2
MIRT578464 Ints12 integrator complex subunit 12 2 2
MIRT578486 Il18r1 interleukin 18 receptor 1 2 4
MIRT580032 Wdr89 WD repeat domain 89 2 6
MIRT580041 Wdr44 WD repeat domain 44 2 2
MIRT580302 Tomm70a translocase of outer mitochondrial membrane 70 homolog A (yeast) 2 2
MIRT581000 Six4 sine oculis-related homeobox 4 2 2
MIRT581273 Rrad Ras-related associated with diabetes 2 2
MIRT582217 Nfatc3 nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 2 2
MIRT582447 Mgea5 meningioma expressed antigen 5 (hyaluronidase) 2 2
MIRT583055 Hoxb3 homeobox B3 2 2
MIRT583626 Strip1 striatin interacting protein 1 1 1
MIRT583683 Amer1 APC membrane recruitment 1 1 1
MIRT583923 Dpy19l1 dpy-19-like 1 (C. elegans) 2 2
MIRT584696 Cep170b centrosomal protein 170B 1 3
MIRT585027 Gskip GSK3B interacting protein 1 1
MIRT585242 Zfp446 zinc finger protein 446 2 2
MIRT586100 Reep4 receptor accessory protein 4 2 4
MIRT587716 Cd300a CD300A molecule 2 4
MIRT588342 Zfp711 zinc finger protein 711 2 2
MIRT588629 Tnrc6b trinucleotide repeat containing 6b 2 6
MIRT589300 Pigh phosphatidylinositol glycan anchor biosynthesis, class H 2 2
MIRT590164 Eno2 enolase 2, gamma neuronal 2 2
MIRT592642 Lpar3 lysophosphatidic acid receptor 3 2 4
MIRT593646 Tbc1d5 TBC1 domain family, member 5 2 2
MIRT595037 Camk4 calcium/calmodulin-dependent protein kinase IV 2 2
MIRT595222 Slc16a1 solute carrier family 16 (monocarboxylic acid transporters), member 1 2 2
MIRT595455 Ptrhd1 peptidyl-tRNA hydrolase domain containing 1 1 1
MIRT595822 Fam227a family with sequence similarity 227, member A 1 1
MIRT596023 Cdk13 cyclin-dependent kinase 13 2 2
MIRT596361 Serpine1 serine (or cysteine) peptidase inhibitor, clade E, member 1 2 2
MIRT596421 Ing1 inhibitor of growth family, member 1 2 2
MIRT596514 Zfp9 zinc finger protein 9 2 2
MIRT599311 Csf1 colony stimulating factor 1 (macrophage) 2 2
MIRT603174 Trp53rk transformation related protein 53 regulating kinase B 2 2

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