miRTarBase - #MIRT588832


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pre-miRNA Information
pre-miRNA mmu-mir-186   
Genomic Coordinates chr3: 157544279 - 157544349
Synonyms Mirn186, mmu-mir-186, Mir186
Description Mus musculus miR-186 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-186-5p
Sequence 7| CAAAGAAUUCUCCUUUUGGGCU |28
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Sp1   
Synonyms 1110003E12Rik, AA450830, AI845540, Sp1-1
Description trans-acting transcription factor 1
Transcript NM_013672   
Expression
Putative miRNA Targets on Sp1
3'UTR of Sp1
(miRNA target sites are highlighted)		 
>Sp1|NM_013672|3'UTR
   1 GATTAGACACCCAGTGCCAGAGACATATGGGCCAATACCCACTAAGCCCGGGATGCAAGGTAGCATGGGTCCAAGAGACA
  81 TCTGGAAGAGAGAGCCATGAGGCATTAATGTGCTTGGTGGTAGGAAGAATTGGGAGATGGTACAAAAAAAAGAGATGGGA
 161 TTGTGGCACCCAATGTCACTGGTGACTCCTTGAAAATGGAAAATATTAGTGAAAATTCTGTTGGTGCCACCCTTTGATCA
 241 GCATTTATCTCATCCCAGGTTCTTCTTACACTTCTTACCCCAGCCTCCTCTTCCTGTTTTTCCCTCTTAGCTCTCCCATG
 321 ATGGATTCCCCCCCCCTTTTCCTAAAGCCATCATGCCTTGATAAATATATATGATCATTGAAATACTTTTTAACAAAAAC
 401 AGATTCTATATTATATATATATATATATATTAAAAAATATATAGAGATGCTTTCACAGGGGCTGGCTGGGAGGAGGAAAA
 481 AGACCATTCTGTGACCAAAATACCTTGGTCTTTTTTTCTTTTCTTTTCTTTTCTTTTTTTTAAAAAAATTGCCTTATTTC
 561 TCAATGGCTGAGCCTTATCATGACACATCAAGCTTTCCTTATAAATGTTGTCCTGGCATCCCACCAGAGTAAGCATTCAT
 641 ACTTTCTGCTTTTAGTTCATATATACATATATAATGTGTCTTCTTTCTTAATTTTGTTATTTTATTTGGGATCAGCTCCT
 721 TGCACTCCTTTCCTGACTTAATCCTTGTCTCCCTTTCCTCACACGATCACTTCCTGTGTTGTTTTTGACAGTGATTCCTG
 801 GATTAGGCACTTGTGTTAATATTTTAAAGACTTTAGTTCCAGCAACAGAGTGGAAAAGCCTGAAGTATAGTCTGATGCTT
 881 GAAGTAGCTATGGAGGGAGTGTTCAAAATACTACTGACGCAAGCACCTTAGTGCTGGAGAGTCAAAACCATCTCCCTTTT
 961 TTAGCTGTTCTAAACATCAGAAATCAGAAGTCTTTCTGTGGAATTGTACAAGAGGCCCTTTGAAGGAGATATACTTCCAA
1041 AATGGTCAAATAGTAGGCAGCAGCTTTCAGTATGAAAAAGATGTTCTCAGAAAAGGAAATAACTCTTAGTCTTGGGTTCA
1121 GAAGTTAGTGGAATTATTCGGATTTCAGTATCATTGTCTTCCAAATAGCTATAAGCCTCAATGTATGGGCTTCTGAGTTC
1201 ACACTCTGAAAGGAAGTACACTTCCTCTTTTGAACATTTCTGTAGTTCCTTCCTATTACTGTTTTAATGTTAAGGAGCAT
1281 CAGCCAGTGAACCTGTTTGGATTTTCCATCCTGTAGAGTGCAAAACTTGTGCCAGTGGCAAAAGTGGTTCTTTAAAAAAA
1361 AAAAAAAGTCCCCCTTAACTCTTCCTTAGATATACTTGGGTGGTTCCTAAGGGAAAGGGAAGCACACAATCATGGGAACG
1441 ATAGCCCAGAACAAAAAGAAATCTTGTCTTACCACTGTGGATTATAGGAGAAACTGGGAGAAATTATCCTGCTTTCTCTA
1521 TGGCCTGATTCCAGAAGAGACTGATCCAAACATTTTATCGGCAGGGAAACTCAGTGCGTTTGGCACTGAGACTTAAATGC
1601 AACCAGAATTGTCCTCAAGGCCCAGCCATTAAAGCATTGTCTCTCTAGACCTTGTGCTATCTTGTCAGAGAGCTTTTCAC
1681 TGTGAGGAAGTATGGGAGTGGTTGAGTGTATGTGTGAGATCTGTTAGGTTGGGGATAGGTTTTCTGTTAGCCAATATTTA
1761 AAGAGACCTGCAATAAAAAATTACCCTGATCTGATAGAGAAACTAAATTGTTTTTGTATGACTGCGTGTGAATGTATTTG
1841 TGCCTGTATATATCTACATGCAGATGAGAACTTATATTTGAAATTGTTGGAAAATAAATTCAGATCAAAACGCCTTTCAG
1921 GTCCAGTATCTAGAAATTGGTCATAAAACTTGGGTATGTTCCTAAGGTCAGTTCTCTGCTTATGCTAAATCGGTTAACAG
2001 TTATGGGGTTGGGGGGATATCCTAGTGCACTTTCTAAAGAAGAAACTATTTTTGTGTTTGAAAATGAAATCAACATCCAA
2081 ATCTATAGTGGATTCCTTATTCTAACTTCTCTATCTTGGCTACAAATAGATTATAGTGTTATTATACATTTTATGTAAAT
2161 TCCATCTAAACTAGGTTCTTGATAAGTTTATGGTATTGTTAGCTTGAAATACTAACATTAGTTTATTCCATTTGGTCCTT
2241 TCTATGCAGACTTAATACTGTTATCAAGACTCAGGAAATATTTCAAGGGACTGTCCCCAAATCACATGTTATTAATGTTG
2321 ACGTAAAAGTTATCTGTCCTCAGCCACCATCACACCTTTTATTCCTAAAGAAAAGCAGAACTCAAACAAACCGTGTTATT
2401 TTACATTTATAATCATGTACTTTGCATCTCTGGAGGAAGAGGGCAGGATAACTCAGTGGAGTGTGCAGTATTTTGCTAGA
2481 AAATTTCAAGAAATAGAATCTTGTTCAGTATGAGGTGATTAGTTACAAGCCGGCTTCGAGATGCTGAGGACTATAAGCCT
2561 TTGGATAAGTTATCTGATGGTGTTTTCTCTCTTGGGTGAAAAGCTGATGATTGACCCTCAGCATCTGCTACATTAACACA
2641 TTACCATGAATTAATTTACATCAGATTCTTTCTGCTTTTTAAATTTCCTTCTCCGGTGTCTTATTTGACCATAAAATGGA
2721 ATCAGTTTCCGCTCTTGTCTCCCTGTCTGTGAGCCGCTCAGCCTTTTCTAAAGTTAAGAGACTACATTCCTCTAGTCAGG
2801 GTCTCATGACTGAAAAATAACCTTTCCCCAAACTTTTGGCCTTCAGAATTTAACATAACTGGCATGCATATTGATTCTGA
2881 AATTCTTTTCATTACATTTTTGTTGTTTGGGGTTTTTTGTTTGTTTTGTCTCATTGAGACAGGGTTTTACTCTGTAGCCC
2961 AGGCTAGCCTGGAATTCACTGTTAACTCAGACTGGCCTGGAATTTTGAATAATTTTTTTGCCTTCTAAGTGTTGGGATTA
3041 CAAGTATATGTCACTATGTCCAGCCTTCATGATTTTTCACTTAATTTTTAAAAAAAATGACGGCACCTTGCAAAGTGGTG
3121 GGTGACCACCAATATAATCTTGTTTTTTGTTTTGAAATAGGTCTTTCTAGGTACCCTTGGCTGATCTGAATTTATATGTA
3201 GTCCAGGATGCAGCGATCTGTCCACCTTGGCCTTTCAAGTGTTAGGGGTTACAGGTGTGTGCCACCACATCTAGCAGAAA
3281 TAGTAATCTTAAAGCATTCATTTCCTTGTCCTAGGCTGCCCATTTGTACTCATTTACAAACTATTTGACTGTGTCATCTT
3361 TTTCCTAGAAAGGACCAACTCACCATCTTCCTTCAGGGACCTCCTAACAGGCACCCTTCGGGGTGCTATTCAAAACGCAA
3441 TATAATGGTTCTTTCTCAGCTTGGTCTCAGAATAAATAGACCCTTTAATCACAGAAAATATAGACTGAAGTATGGGATGA
3521 AGGTGTTATAATTTGGGGAGGGTGGGAGACTAAAGCTGTAAATTACTATGGCTGATTTATTTCTACTATATACATATATT
3601 TTTTGCTTTTGTATATCCTATATAGAAAACTAAGCATTGTATTTTTTTAACAAATCTGAGAAAGCACTATGAACTGCAGG
3681 TGTTTGACTTTCAGAATATATTTTGTATTGTTAATACTTCACATTATGTGAATACTGGACGCTGCAGATCTTTGTAGGCC
3761 GCAATAAAATTGGACACTTTTTGAGGGGTTCTTTGGAGGTGCTAATCAGGCCTCTGTTACACTTTGAAGGAGCCCTGGTG
3841 CTACATGATTAACGTTTCATTCAATTTTAAGTGCCCTAATGTCTTTTTGGACCTTGGGATCAGTTGAGAAGTGATTTAGA
3921 GATCAGGTACCGAGGGAGTGAGGATAGTGTGCCTGCCTCAGCTAAGGGCCTCTTTCATGGTTTTACTTTCCATAGACAAA
4001 GTTGGGTAGCCTCTTGGGGTTGAAAGCAGAAATGTTGGAAATCTCAGAGTTTCATCTTCTCTTAGAAGAGCCAGTAACTT
4081 ACAAGGATAAAGGTAGCAGCAGTTACTTTGGGAGAGGAGGGCATGGCACTTTTCCAACCCGGAAAACATTGCTTTGAAAG
4161 CTGCTGATAAAGCATGGGCAGGTTATTAGTTTGGTTTAGAAGACTGTGCGTCTCTTGGCTCTGCTCCGGGGTACAGATCT
4241 CATCTAGGAGGATGAGTAGAAGCTTTGAGGTTGAGCTGTTTCTTGTCTTTGTCTGCCTTCCTTAAATAACATCCCCCAAA
4321 AAGACATTAAGCAGCCTTTACGTTCCTACTCCCTCTTCAGACTTGGCTCATGTGTTGTAGGTTGAAGACTGGGAAGAGAG
4401 AAGAGGGGCAGTCTTCGGCTCCATTCCTTCCCTAAGTCATCTCTGTTCAAACTTCCACACACTCAGAAGGATTTCTCCAC
4481 AGCACTCATGTGAGAGAGAAGCTGCTTCTTTTTTTCCAATTTGCCCCTTCTTTATCAAACAGCCCAACCTTCCTTGTCTT
4561 GGGATTCATCTGTCCTTCAGCTCCTCTAAGTCTCAGCTCGTGGAGGTAGAGGAGCCTGGCAAATTAGGCTGGGGCTACAG
4641 ATAGAGAAGACTTGAGGTACATCTGCTTGGTTGTGTGCTTTCCTATATCTTGCCTTCAGGAATCACACTGTGCCTTCTCC
4721 ACTAGGGAACTGATTCTATCTACACAGCTTTTCCAGTAACTGCTCTTGAAAATTGTGGGTAATGGCAGTTCTTCATACTT
4801 CTGATGACTCTTTTCTCCCAATAACATCCTAATATCCCTTACCTAGCTAAAGACTAGGCACAAAGTCAGAATATGTTGAT
4881 AATGGTCTGAGGAAGCCAAAGGCAGCTGCCCTAGGGCCTGCTTCCCTCTCCCAAGTCTGTCATCTCTGGGCAGAGGAGTA
4961 GTGCTCCAATCTGGTGCCTAAAACCAGTTTATCTTTCTTAATACTGGCAATAACCAGTCTCTGTACCACTGTTGCCTTTA
5041 AACCTTTTAATCTCACGATGTTTGTTGTGGGTTCCAATCCAGTTATCACCAGGGCTGTGAGGGTAAATACTTCTAAACAG
5121 TCACTTACCTTGTTCTCTGCCCTCACTCTTGAGTATGTATGATGTTAATCTCTTGTCTTCCTGTTCCTTTTGTATCTTCC
5201 TCGTCTCTCTCCCCCTAATTTTGTCCCTGGGTGTTTTGGGGATCTCAGTATCTCTTTCTCTATATCTCTATCTCAATCTC
5281 TCTGCCTTTTGTACAAAGATTAGTTTCAATGTAGTCTGTAGCTCCTTTGTAAACCAATTAAAATTTTTTTAATAAATCAT
5361 GTCTCTGGTGTA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ucggGUUUUCCUCUUAAGAAAc 5'
              ||||||   ::|||||| 
Target 5' gtggCAAAAG--TGGTTCTTTa 3'
 1335 - 1354 142.00 -8.00
2 
miRNA  3' ucGGGUUUU----CCUCUUAAGAAAc 5'
            ||:::||    | | ||||:||| 
Target 5' ggCCTGGAATTTTGAATAATTTTTTt 3'
 2994 - 3019 142.00 -7.30
3 
miRNA  3' ucGGGUUUUCCUCUUAAGAAAc 5'
            | ||| |  | ::|||||| 
Target 5' aaCGCAATATAATGGTTCTTTc 3'
 3434 - 3455 140.00 -5.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0000215
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013581. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
46 mmu-miR-186-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577608 Stxbp4 syntaxin binding protein 4 1 1
MIRT577884 Qpct glutaminyl-peptide cyclotransferase (glutaminyl cyclase) 1 1
MIRT578137 Noc3l NOC3 like DNA replication regulator 1 1
MIRT578249 Mnat1 menage a trois 1 1 1
MIRT579147 Ces2g carboxylesterase 2G 1 1
MIRT579219 Ccdc138 coiled-coil domain containing 138 1 1
MIRT579578 4933427D06Rik RIKEN cDNA 4933427D06 gene 1 1
MIRT580348 Tmem33 transmembrane protein 33 1 1
MIRT580592 Tbl1xr1 transducin (beta)-like 1X-linked receptor 1 1 1
MIRT580668 Stk32a serine/threonine kinase 32A 1 1
MIRT580744 Srek1 splicing regulatory glutamine/lysine-rich protein 1 1 1
MIRT580779 Sp6 trans-acting transcription factor 6 1 1
MIRT580847 Sltm SAFB-like, transcription modulator 1 1
MIRT580944 Slc26a5 solute carrier family 26, member 5 1 1
MIRT581214 Samd4 sterile alpha motif domain containing 4 1 1
MIRT581276 Rprd2 regulation of nuclear pre-mRNA domain containing 2 1 1
MIRT581520 Ptprb protein tyrosine phosphatase, receptor type, B 1 1
MIRT582203 Nfia nuclear factor I/A 1 1
MIRT582893 Ildr2 immunoglobulin-like domain containing receptor 2 1 1
MIRT582996 Id4 inhibitor of DNA binding 4 1 1
MIRT583079 Hnrnpa3 heterogeneous nuclear ribonucleoprotein A3 1 1
MIRT583125 Heca hdc homolog, cell cycle regulator 1 1
MIRT583739 Epm2aip1 EPM2A (laforin) interacting protein 1 1 1
MIRT584127 Crispld2 cysteine-rich secretory protein LCCL domain containing 2 1 1
MIRT584166 Cpox coproporphyrinogen oxidase 1 1
MIRT584531 C230081A13Rik pseudopodium-enriched atypical kinase 1 1 1
MIRT584646 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT587978 Armc1 armadillo repeat containing 1 1 1
MIRT588832 Sp1 trans-acting transcription factor 1 1 2
MIRT590758 Acbd5 acyl-Coenzyme A binding domain containing 5 1 1
MIRT593146 Mmp12 matrix metallopeptidase 12 1 4
MIRT593495 Gjc3 gap junction protein, gamma 3 1 1
MIRT594880 Lrig2 leucine-rich repeats and immunoglobulin-like domains 2 1 1
MIRT594898 Ifi204 interferon activated gene 204 1 1
MIRT594932 Gdi2 guanosine diphosphate (GDP) dissociation inhibitor 2 1 1
MIRT594945 Fmnl2 formin-like 2 1 1
MIRT595197 Emc3 ER membrane protein complex subunit 3 1 1
MIRT595343 Frmd4a FERM domain containing 4A 1 1
MIRT595402 Arsk arylsulfatase K 1 1
MIRT595430 A630033H20Rik RIKEN cDNA A630033H20 gene 1 1
MIRT595947 Nelfa negative elongation factor complex member A, Whsc2 1 1
MIRT596153 Opn5 opsin 5 1 1
MIRT601390 Utp23 UTP23 small subunit processome component 1 1
MIRT602153 Intu inturned planar cell polarity protein 1 1
MIRT602577 Colec12 collectin sub-family member 12 1 1
MIRT604817 Nr5a2 nuclear receptor subfamily 5, group A, member 2 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-186 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-186 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-186 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-186 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated
miR-186 Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 down-regulated

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