pre-miRNA Information | |
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pre-miRNA | mmu-mir-466h |
Genomic Coordinates | chr2: 10514891 - 10514971 |
Description | Mus musculus miR-466h stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-466h-5p |
Sequence | 11| UGUGUGCAUGUGCUUGUGUGUA |32 |
Evidence | Experimental |
Experiments | Cloned |
Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | Slc1a2 | ||||||||||||||||||||
Synonyms | 1700091C19Rik, 2900019G14Rik, AI159670, Eaat2, GLT-1, GLT1, MGLT1 | ||||||||||||||||||||
Description | solute carrier family 1 (glial high affinity glutamate transporter), member 2 | ||||||||||||||||||||
Transcript | NM_001077514 | ||||||||||||||||||||
Other Transcripts | NM_001077515 , NM_011393 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Slc1a2 | |||||||||||||||||||||
3'UTR of Slc1a2 (miRNA target sites are highlighted) |
>Slc1a2|NM_001077514|3'UTR 1 TGACCCGATTCTCAGCCAATTCTTGAATAAACTCCCCAGCGTATCTTATGGTAAAAGATGCTCTCTAAACAGGCTTCCTT 81 TACAAAAGGAAAAGATGCATATGTTTCTATGTTTACTTAATCTGTTAGCTGAGGCATAGAGGAGCTGTGTACACCCGTGA 161 TGATAGGCACCGTGCTGTGTCTTTGCCAAATAATGCTTTATAACTGTCTAATTTTCTTACTTGTGTTATTGTTTGAATGG 241 ACGCTGCTGGAGAAACTACTTTGAATGGAAGACGTGTTCTTGCCAGCTTCCTTTTGTCTCAGTGCAACACTGTGGATGCT 321 ATTTTTTTTCCAAGGGGACAGGATGAAAGCAGTGTGGTACCCTCCAGGGACTTAAGACAACGAGCACACATGTGCTGTCT 401 TCCTTAAAGTGTTCATGGCTCACCTTGTTCTACACAAAAGATTCTGCTTGAAGCTTCTAGAATTACCTCCCCTCCAGCCT 481 GTAGAGAGTTGGCACCTACATTGATTGAAAGCATCTGCCTGGGGGAACTCTGACAACTTACCTTTTTGTGCACTGTCTAA 561 GTAGAATGTTTCAGAGTTTCATGGGTGCCCATACTCTGAAATGCAGGTGACCATAAAGGCCACAGCTCTGCCTTTCTGTG 641 TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG 721 AGATGTGTGTGTGTGAAAAATGTGAAATTTCAAATTTGAAAATGATCTAGGAATCATTTGCATATTACCACTGTATACCT 801 TGAGGAAAGTGAAGGAGCATAGTCAACTTGAATTTTAGTCAGAATTAAATATTCCAATTCTCTTGTTTGACTTGCATTCG 881 TAGAGCATATTGAACTCAAGTGTGATGATCTGATCTGGTTTTTTGTTCCACCCATAGATTCTCACTTGAGTTCCTCACCG 961 AAAGATATTTCTAACACCCAAGTCACTTCCATCACATCCTTGTCTGCTTATATGGCTGAGCACTTCAGCTTCCTCACATT 1041 AAGAGACACACGTTGGCTATGACTCTTTTCCTATCATCCTGGTATATATCTAATATGTAGAAAGTGTTTGTTATATTAAA 1121 GCACATGAAAAATATCACAAAACACAAAAGTTCTTTGGTGAGAAGGGAACAGAAACCACACATGTATATAAAATCCTATA 1201 AAGGTTCCTATGACAGAGGCCAGGCAGCCTCAAACCATCAGATGGGGACCAAGAACGGCTCTACACAACTTTCCAGATAG 1281 AATCCTTTCCGGGGACTGTGAAAGTCTGAAGGAATAAATAATATAATTGGCACAGCATGAGGGTTTGCCTGTGGTAGGCA 1361 GCCAACGAATCCTTAATGATGGGTTGTGCAAGCTAAAATTGTGGATGTCTCTATTATTATCTTCTGTTGAAGAATGCACA 1441 GTATTTTCCTAAGATCCTGGGTTTAACGGTTAGCGGGGAACTGGAACCACTGAAAAACAAAACAAATTCAACTTGAAGGA 1521 GTGTCGGAACCCAAGCCATCAGTCAGGGCATGTGTGTCCTCTGAAGCAGGCAGAGCTGAGGAGACGTTCATAAGCAGTAA 1601 TAGGACCCATTGCCTCTGAAGAAGGGCACGGGGCCATCGTTTCTCCTCTTAGCCCCTCTTGTGACTTCTCTCCTTCAAAT 1681 CAGACAGCTCAGACACATACAAAAGACAAGAGATTCCTGTTGCTTTATCTCATGAACCCTAACTGTTTTTAAACAAGGAT 1761 CACTATTGAGCAGTATTAAATGAATTAGTTTGTTCTGTTTCAGGTTTTGACTTTTGAAACAAGGTCTTTTTATGTAGCCC 1841 AGAATGGCTTCAAACTCTCACACCTACTGCTCAGCCTCGGTTCTAAGATCACAGGTGGGCACCACTGCATCGGCTGGCAG 1921 AACTCTTGTTTATACTTTTCTGAGAGACAGTGAACAGGGTAGCAGTGACCAGCTGTGAAACCCTGAAGGAAGACAAGAAG 2001 GGCCAAAGGGATTCTGCTCAGCTGTGGAACTATTCTAGATAGCCTGTAAGTGGTACCCCAGGGCTCTCCTATGCTGAGTA 2081 GATGTATCTAGGCAAGGCATCAGGAGCATGCTGGGTTTTGCTGTGTTGGGGAGGGAAAGGCCATTCCATACAATGAGTCT 2161 CCCCATACAAGAAGAAACAAGGCCACGTGGGTACTTGAACATTGCTCTTTTCTTAAAAGCACTCACAGGATGACAGTGTT 2241 TGTCATCTCAGATTCTGCCTGAAAATCTTTCTAATTTGTTTTATCTTCACCACCCCCCCCCCAGCAGTCCTGTTAGCTTA 2321 TGTAAAGAAGACAGAGCTTTCAGGAGAACAAAGGCACATTATTTCTCAAGTCAGGTTTTGCTGACTACATATGCTGTCTC 2401 AAGAGAATATGCTAGGGAAATCACATTTACTGAAAATTTAATAATTTTTATTTAAAATAAATAATTTATCAAATTGGAGA 2481 CTTCCCTAAGAAATCTAGAATGTGATGGAATTGACATAGTACTGTGTCTTGCTTTTGTAAGCCTTGTTAACCCTTTCCAT 2561 GCCCATTGGAGATTCCCTTGCCTAGAAAAGGTGCAGGAAGCTGAAGAAAAAAAAAACAGATACAGAGTGTGTTCTAGTAG 2641 CCTTAGTCTAAATAGGGTACCTGTCACCGTGTGTGTATTAATAGAGCCATCGTAGATCTGAATGTCCATAATGATGATTA 2721 ATCTTTTATGTGTAGTTCTCTCTCTGCCTCTGTCTCTGTCTCTCCCTTTCATATGCACACACACACACACACACACACAC 2801 ACACACACACACACACACACACACAGAACATGTGTGTCACATACACTAACTCCAGGAACCAGGGAGGTTTGTGTATTGGG 2881 GCTTTGGCAGTGCTATTCCTGTAACGGATTCAGTTAATACTTACAAAGAATCAAAATCATTTCAGAAAAAACAAACAAGT 2961 GAGCAGACGTTCATCTTCCCTGTCTCGTCCTTTCCCTATAAGAAGTAAGACAAATCATCATTTTATTAACTGCTGAGTAA 3041 AACTCAACAAAGTCAATTCAGCCAGTGAACAGCATGGTTGTTTCGAAGGTAAAATTTCACTGTGGGCTTCTGAGGGTGGC 3121 GGGACACCAGAGAATCCAGGCAGCCATCAGGACCTAAGCACACAAAACCTCAGTAAAGGCCTGTAGGTGATTTTAGGACC 3201 ATGAGATCACTACTGATTTGGTTTTTGAGCTTTTTTTTGGGGGGGGGGTGGTAATAAAAGCCTGTGTGGCCTCATTGCCT 3281 GTGGAAAGTTTTATGTTCTATAAAAGACAGCTAGCCTTTTCTCCTCACTAGTCACTAGTTAGTTCGGACATTTACAGAGC 3361 AAAGGAAGGACTCCAGAATGTCTCCAGATGAAAACGAAGAAAGCACGGAACTGTGTATGAGGAATTCAGTTTATGGATCA 3441 GTCCCTGCTGGGGGATGCTGGGATCTCAGTGGTTCGCTGATTAGGGTCAACTATGGCTAGAAAATCATGGCACATAGAAG 3521 TGCACGTAGTCAGACACCCTTCAGAGAGTTGAAAGGCCTCAGAAGAAAACCCAACCCTGCCTTGCTGCTGTGAGTAATCT 3601 TCCCTTTGTTCTCCGAAGTAAAATCTTATTATAATGTTCACTTTTGTCAGGGTCTAGGCTCTTGCCTATGCATTATTTTC 3681 CTCATGCAACAAAGCAATAAACCCAAGTAGGTATTCGCATGCTCAGGCTCCGATAAACAAGCCGGAGCAGCTCAGGCCTC 3761 CTTTGATGACTAAAATGGCAGTGGTTACAACCCCGGGGCAACCTGCCCGGTTTGCTGCTCTCTTTAAGGACCAATCTGTC 3841 TGCCCTGCTTTTTTTTTTTTTTCTTTTCTGCTTATTCTGCAGCTGAACTCATGATCTATCAGGCAGGACGAAGGACCAGA 3921 TGGGATTTTCTAGTACTGGGATACTTTCAGGGCTTCAGGGTCCAAGGGTCTTAAGGAGATAAGGTTAGCGTCTGAAGCAA 4001 GGGTTTCCTAAACCAAGTTGTCACTTGAAGATTCATGGTCACCGCATCTACAGTAGTCTCTGGTTAAACCCAAAAGAAGG 4081 ATTACTGAGTGCCAACCCAGCCAGGTGGCAACTGTGACAGCTAATTTTCCGTGGGTGGAGTGAGATAAAGTCTCATCAAG 4161 AACACCAACCAGCTTAGGATTTTCCCTAAAACTCTCTTCCCAGTTCACTGCCCTCGCCCTTCCCTTTCCTTTCGTTTCAT 4241 TGCTTAAGAGACTGCTTAGTAAGTGGCCAAATAGTTAAGAGGGGGTTGGCTTGGAAGCAGATAACAGGAGATCTTAACTA 4321 TCAGTAATAGCCAGTCTCACCGCCTCACACCATAATCAGGCAATGACACAGAGATGTTTAAAAACAACGTGGCTTGGGAG 4401 AGGAAACATTTGATTAAGACACTCCCCATCTGTAGCTGTAAGGCTCACACAGCCACTGTGTGCCCAGGTTTCTTGCACTT 4481 CCTTCTGAAATGAGAAGGAACTGGACCAACTGCAGTTTTCTAATCTCCTTCCATCTTACTGTTCTCAAACATGACTTATT 4561 TATATTGTAAGTTTGAAAGACACACAAAATGAATATTCAATTTAGGTGTTTCCTTGTCCATGATGAGAAAACTCCAACTC 4641 AAGCTTCCATTCATTCCATACTTTACAAGCTTGGAGAGTGGCCACAGTAACCCAGTTTCCAGCATTCAGACATCAGTAAA 4721 TATCCTTTGGTTAAAATCCCTATTCCCCAAACTCACTGTTCTCACGGAAGTGGTGATTTTGCTCAGGGAAATGTTCTGCA 4801 ACATCACCGTGAGGGACCAGACACCTGGAGTCTGAATTTTGAGATCTTGCATCATACCTGCTGTAGCAACAGAGCCCACT 4881 CCAAATTCTCAGCCCACATCATTTTCCCCAAACAGTCCTAATTGCCAACCCTGTGTGGGAATTTGGGAGCCTCTGGCAAA 4961 CAAGCTTGAACACTCCTGACATTTGGTGCAAGATTTACACAACAGCAACTGTCTCCACACACCTTTCCCGCCAAGTACAT 5041 GCAGACGTATACATCCATTCTGCGCCTTCCCCCACCCCCGCCCCCACCCCCGCCCCCTGGAGAGAGGATTTGATTGATTT 5121 CATTAGAAAGTTCTCCTCTTGATTTACATGCATAACAGACAGCACATCATTGGGAGAGATGCTGTCACTTATAAGAACGG 5201 AGTTTTCCCATTTGCCCCATGTCCGTCCATGTTCACAGAGTGTCTAAGTCATTACAGCAGAGAGGGTGGCTGCACCTAAC 5281 CTTCCCTGGTTCTGTATAAAAGGTCATAGCTTGCATTTTTTGCTCTCTGACCAAATTTCAACCAGAGTTAAATGGATGTA 5361 TTTAGTGTGGTGAACATCTTCATTGCCAGAAAAGATTTCAGCTCTTCAAATTTGAGGGAGTATAGCCAGTTGGGAAAGGG 5441 CTAATTATTGTTAGTGGTGATGGTGGTTGGGTTTTTTTCTCCTCTCAAGGTGACAGAAGATCTATGGTGAGAGCAGAAAG 5521 ATGTTTCTTAATTGACCTACTTAAGGTCACTGCAGAAAAGGGGGAGAAGTCCAGAAGGTCCTGGGAGATTGGGTTCACAC 5601 TAACACTCTGAATCATTTCTGATCAGTTTTTTTTTTTTTTTCTATGCTGAATCTTGTCTTATCCTATGATCCAGTTACCA 5681 GTTTTGGTATTTTTAAGTCTTTCTCATTGTATTTTGGGGAATTTTTAGGCTGAGAATAAAATCTACTATTGGAACAGACC 5761 TGTGCTCTGCAGAGGCATCACGTGACCCAGACAACGGGTGGCAGAATATCTGGGTTCCCCTGGAGACACCCCCAACCCCT 5841 GGGTGAGCTTTAGAACCTAACAACAGACTGTTATCCTTATCACCATCCTCTGGGACATGGGGGATGATGCAGTCAGAGCC 5921 TTGCTACTTCTGGGCTTTGTTTCTCTCTATGGCAACCATTTTATCCTGCCTGGAAATGGCTTCCTGCCCTACGTGGAGAG 6001 CAGGCACACTTACAGCCAAGGAAGTGGTCAAACAGATCCCTAAGTTCTCTCCATGCTACAACAAATTCCCTAATACACAC 6081 AACCAGAAACTCAGCTCCTGGGTGGCTTAGTACCCACAAGGTCACTTTCCTGTTCTAAGAGCCTTCGGTATCCAGATTCT 6161 TCTACAAACCACCTCAACTCTTTCCTCTTTCTTCCCTCCTCCATAAAATCTCAGCATCCCACCCCCACATTGCACAACAG 6241 TGATCAGATCAGCTGGTCACTATAGAGATTTGTATATATTAAAACAAACCTACTTTTTGGAGAATTTTTTGTATATTCTT 6321 TTCTATTTGTTTTCTACAAATTGAGGACAGAGCTGGCAAACTGTGAATCTAATTTTATCTTGATGCCAGCAGATATATTT 6401 TAGCTTCCTGGTATGAATCTATGTTACTAGGGACACCATATCACCCTCTGATCAGTATTGCACCTCCAATCTACAAAGTT 6481 AGTTTGGCCCAATCTTACATTCTCTCTTCCACAAATCCCCAAATTTCTTTGCTATTATTTATACAGAGAATATTTATAGT 6561 CCTGGATATTACAAGTTTTAATGTTAATTTAACTTTTAATAACATTACACATTTGTATCTACTCTTCAAAAATCATAAAG 6641 ACTCAGAGTAGGCTGATATAGTCTCTTTGGTATCTGTAATTTTTTCAGACAGATATTTTCTTATGATATTTTCTATAAAA 6721 CCACATAGATAGAAGTATAGCTAATTAGAGCACACATTTCTATAGCAATTTGGGGTATCAACTGTTTCCTGAGCATACAG 6801 ACACATTTGAGAAATTTTGGAACTCAGTCCTTAATGATGCAATGAATAACTTGACCAAACTCAGAACGAAGAATTGCTTA 6881 AGCCCCTTAGGTAGTTAGCATATACAACTGAACAGTTAATCCTTCAGCATCAAGGAATGGTGCTGACTGCTGTTTCCCGA 6961 CACCAGTTCTTGTTTGCCCAATGGACCTTTCTAATTTTATTTTTCTATAAGAAGAATAAAGGCCGGTGCTAATAGCGGGA 7041 CCGCTTCTCTATCAGAATCTCCCATGCATTTCTAGATGTTTATGTGAAAATTGGCCCTAATGAAACAGAGTAGCAAAGAC 7121 TGAGGATGGTTAGGAAGAAAAGAATTCAGGACAGGTTTGGTGCAGTTTTAAGTCTTGCCAAGCAAAATGATAGCAAAACC 7201 TCACATTGTTATATGAATTGAACATATATTTTTAAGAAGAAATGAAAGACAACATATTTTTAGTACCCATTCAGAATCCA 7281 TGCTGTATCCCTGGGGGCGGGGCAGAGCTGGGAGTTAGAGGCATACTGAGATTATTTAGCATAAACTCAAATACTTTATC 7361 CTCAGGTAGGCAACAAACTTATGTAACCTTCCCACAAGGTAAACCAGTAGCCACGGAAAACACAAGGTGTCTTTTCTCCA 7441 CCGAACACACACTCTGCAATGGTGGGAGGACTGGTATTTTACTGTGTTTGAAAGAATAGCCTGTTACATAGATGACATAG 7521 TAGGATGTATTCAAACCCGCTCCTCATTAAGCGAACAGATTAGCTGTGCCCTCAACTGGCTACGATTGTATCTGTTTTCA 7601 CCGCTAGCTATCAGAGCATGAGTTTCTTGTGTGTACACGCTGATACTTGGTTCACGCATGAAGACAGTGCCTATGCACTT 7681 TGTGTAGCTATCATGTAGGTATTTGTGTGTAATTTCAGTGAAACAATGTATAGACGTAATTTAATGTAACTGTTTTTCTT 7761 GGCTGTTTATCTAAATGCAGTAGATATGTCGGTTGCCGTTTGGAAACCTTCCTTTGCAGTCTTAGGTGTCGTCTAAGAGT 7841 TATTTCTCGTTCTCAATGTGACCTGAGTGAAGAACTTAACCTATGAAACCCCTGTACTCATGATTGTTTGCAAATCTCTG 7921 AGTCCAGCACCTTTGTAAATACAGCCCCGAGGAGGAATGGGGGTGAATGTGTTTCCTGTTACTCAGTGAAATATCAGCCT 8001 ACGCCTGCATCATTCCCACTCAGTGCACCAGTGGAACTTGTGGTTACCAAGTCTGAGGGAGGTTTAGAATGAAGACACTC 8081 GTCTCTCCTCCCTCACTGGTGGACTGGGCTTAAAGAGACAGAAAAAAAAATCAAAAAGTCCATCTCCTCTTCATACTCAA 8161 ATCATGTCTTCTGGGGGAAGGGTCAACCTCTCTGGTTGTCAGATCTTCACTTGATGGTGACCTTCATCATGGCCTAAACA 8241 TCATGGCCTGAAGAACCTTGTTACCTTTGACTTCCCGAGTTGGAGTACAAGAATGTTTTTATTGATTAGTAGTCATGCAG 8321 GTGAGCCTGAGCCTCCCCCGAAAGCACAGTTCTGGCAGCAAATTGCACACCTGTGAGAGAACAACAGAGTACTAGAAGCA 8401 CACGAAGGCTCAGGGCCAGCAACAGCCAGGGGCCAGCAATGGTTGAGGACCAGCAAAGGCCAGTGGTCAACCTTGGCATT 8481 GGCAGAGGACCAACCTTTGATGGACTGATCTTGAGTATGTGACAGGAGGCTGATCTCCTCTACTCAGCCTGCTCTGCTTG 8561 CCTGTGTGGAGTAGGTGGAGATGAGATTAACAATGGCAATTAGATGTGATTTTGCGAACTGCATTCTCACTAAATACCAT 8641 ACTTTGTAGTTCCTGTAACCCCTCTCTTCCCCACAAAGACAGAAGAAACAGCAATGTGCTCGAGAGTACCCCTCCCTTTC 8721 CTCACAGATCATTGAGAGGTGAAGCAAGCACACTCATTTTCAGGGGCTTCCCCAGATGTGTGGAGTGAGGGGATATGGTG 8801 ACTGTGCCCTTCAAATTATGGATGAAAAAAAAAAAAGTACAGAAGGGAAGGAGACTTTAGGCAGGATTATTTTTGAATGA 8881 AAGATCAGATTAATGTTTTCCCTTCCGCTCACAGTCCCCGTTGGTCTGGTGTCATGTAGCATAGTGCGTCTATAGCACAT 8961 GGTATTTAAAGACTAATGAATGCAAAGAGACTAATTTGGGGGCTGTTTCTTATATATGGTACTAGGAAATGCCTTGTTGG 9041 CAAAGCACACCTTGGGTAGTTGAGATCTCCTGTGTTCGTCTTTAGCTTTCCTTGTGATGTAGACTCATGACTGTAACATT 9121 TCATGTGTAAAATAACTGGATATTCTTCATATGATGGAAATAACCTGTGAATTCAATCTTTCACTAAGTGTTTTAACTAC 9201 TGTGTATATATATCTCTCTCATCAATAAATGTAGATTGCAATTTCTCTTT Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | Liver |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in ERR266281. RNA binding protein: AGO2. Condition:A_Liver partial hapatectomy 48h
HITS-CLIP data was present in ERR266295. RNA binding protein: AGO2. Condition:B_Liver partial hapatectomy 1h
HITS-CLIP data was present in ERR266300. RNA binding protein: AGO2. Condition:B_Untreated
... - Schug J; McKenna LB; Walton G; Hand N; et al., 2013, BMC genomics. |
Article |
- Schug J; McKenna LB; Walton G; Hand N; et al. - BMC genomics, 2013
BACKGROUND: Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs. RESULTS: miRNA recruitment to the RISC is highly dynamic, changing more than five-fold for several miRNAs. miRNA recruitment to the RISC did not correlate with changes in overall miRNA expression for these dynamically recruited miRNAs, emphasizing the necessity to determine miRNA recruitment to the RISC in order to fully assess the impact of miRNA regulation. We incorporated RNA-seq quantification of total mRNA to identify expression-weighted Ago footprints, and developed a microRNA regulatory element (MRE) prediction algorithm that represents a greater than 20-fold refinement over computational methods alone. These high confidence MREs were used to generate candidate 'competing endogenous RNA' (ceRNA) networks. CONCLUSION: HITS-CLIP analysis provide novel insights into global miRNA:mRNA relationships in the regenerating liver.
LinkOut: [PMID: 23597149]
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Experimental Support 2 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | C2C12 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast
... - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell. |
Article |
- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al. - Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
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CLIP-seq Support 1 for dataset ERR266281 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | Liver / A_Liver partial hapatectomy 48h |
Location of target site | NM_001077514 | 3UTR | AUAUGCACACACACACACACACACACACACACACAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23597149 / E-MTAB-1612 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset ERR266295 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | Liver / B_Liver partial hapatectomy 1h |
Location of target site | NM_001077514 | 3UTR | CAUAUGCACACACACACACACACACACACACACACA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23597149 / E-MTAB-1612 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset ERR266300 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | Liver / B_Untreated |
Location of target site | NM_001077514 | 3UTR | CAUAUGCACACACACACACACACACACACACACACACACACACACACACACACACAGAACAUGUGUGUCACA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23597149 / E-MTAB-1612 |
CLIP-seq Viewer | Link |
CLIP-seq Support 4 for dataset GSM1385342 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast |
Location of target site | NM_001077514 | 3UTR | UCAUAUGCACACACACACACACACACACACACACACACAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 25083871 / GSE57596 |
CLIP-seq Viewer | Link |
87 mmu-miR-466h-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT577339 | Zfp157 | zinc finger protein 157 | ![]() |
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2 | 2 | ||||||
MIRT577488 | Tns4 | tensin 4 | ![]() |
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2 | 6 | ||||||
MIRT578067 | Oxsm | 3-oxoacyl-ACP synthase, mitochondrial | ![]() |
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2 | 2 | ||||||
MIRT578343 | Ltf | lactotransferrin | ![]() |
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2 | 2 | ||||||
MIRT578743 | Gm4841 | predicted gene 4841 | ![]() |
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2 | 4 | ||||||
MIRT578822 | Fermt1 | fermitin family member 1 | ![]() |
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2 | 2 | ||||||
MIRT579127 | Chrnd | cholinergic receptor, nicotinic, delta polypeptide | ![]() |
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2 | 2 | ||||||
MIRT579135 | Chrna1 | cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) | ![]() |
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2 | 2 | ||||||
MIRT579305 | BC021785 | major facilitator superfamily domain containing 4B5 | ![]() |
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2 | 4 | ||||||
MIRT579417 | Agtrap | angiotensin II, type I receptor-associated protein | ![]() |
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2 | 2 | ||||||
MIRT579732 | Zfp92 | zinc finger protein 92 | ![]() |
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2 | 4 | ||||||
MIRT579978 | Wrn | Werner syndrome RecQ like helicase | ![]() |
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2 | 4 | ||||||
MIRT581035 | Sim1 | single-minded homolog 1 (Drosophila) | ![]() |
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2 | 4 | ||||||
MIRT581532 | Pten | phosphatase and tensin homolog | ![]() |
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2 | 2 | ||||||
MIRT582312 | Nab1 | Ngfi-A binding protein 1 | ![]() |
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2 | 4 | ||||||
MIRT582843 | Itga9 | integrin alpha 9 | ![]() |
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2 | 4 | ||||||
MIRT582976 | Igf2 | insulin-like growth factor 2 | ![]() |
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2 | 4 | ||||||
MIRT583470 | Foxk1 | forkhead box K1 | ![]() |
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2 | 4 | ||||||
MIRT584662 | B4galt6 | UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 | ![]() |
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2 | 4 | ||||||
MIRT585220 | Zfp488 | zinc finger protein 488 | ![]() |
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2 | 4 | ||||||
MIRT585299 | Zfp26 | zinc finger protein 26 | ![]() |
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2 | 2 | ||||||
MIRT585311 | Zfp248 | zinc finger protein 248 | ![]() |
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2 | 4 | ||||||
MIRT585835 | Slc6a17 | solute carrier family 6 (neurotransmitter transporter), member 17 | ![]() |
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2 | 4 | ||||||
MIRT586077 | Rhobtb1 | Rho-related BTB domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT586329 | Pgm5 | phosphoglucomutase 5 | ![]() |
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2 | 4 | ||||||
MIRT587381 | Dzip3 | DAZ interacting protein 3, zinc finger | ![]() |
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2 | 2 | ||||||
MIRT587611 | Cml2 | N-acetyltransferase 8 (GCN5-related) family member 2 | ![]() |
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2 | 2 | ||||||
MIRT587631 | Clec7a | C-type lectin domain family 7, member a | ![]() |
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2 | 8 | ||||||
MIRT587725 | Cd28 | CD28 antigen | ![]() |
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2 | 2 | ||||||
MIRT587758 | Cd200r1 | CD200 receptor 1 | ![]() |
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2 | 2 | ||||||
MIRT588012 | Akap7 | A kinase (PRKA) anchor protein 7 | ![]() |
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2 | 2 | ||||||
MIRT588296 | 1700019G17Rik | N-acetyltransferase 8 (GCN5-related) family member 4 | ![]() |
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2 | 2 | ||||||
MIRT588555 | Uhrf1bp1l | UHRF1 (ICBP90) binding protein 1-like | ![]() |
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2 | 2 | ||||||
MIRT588689 | Tet2 | tet methylcytosine dioxygenase 2 | ![]() |
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2 | 2 | ||||||
MIRT588893 | Slc1a2 | solute carrier family 1 (glial high affinity glutamate transporter), member 2 | ![]() |
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2 | 4 | ||||||
MIRT589021 | Rnf11 | ring finger protein 11 | ![]() |
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2 | 2 | ||||||
MIRT589101 | Rasal2 | RAS protein activator like 2 | ![]() |
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2 | 4 | ||||||
MIRT590106 | Etv3 | ets variant 3 | ![]() |
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2 | 2 | ||||||
MIRT590170 | Elovl6 | ELOVL family member 6, elongation of long chain fatty acids (yeast) | ![]() |
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2 | 4 | ||||||
MIRT590929 | Tacr2 | tachykinin receptor 2 | ![]() |
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2 | 2 | ||||||
MIRT591061 | Ptpre | protein tyrosine phosphatase, receptor type, E | ![]() |
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2 | 4 | ||||||
MIRT591144 | Nsun3 | NOL1/NOP2/Sun domain family member 3 | ![]() |
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2 | 2 | ||||||
MIRT591210 | Mdm2 | transformed mouse 3T3 cell double minute 2 | ![]() |
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2 | 2 | ||||||
MIRT591429 | Car10 | carbonic anhydrase 10 | ![]() |
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2 | 4 | ||||||
MIRT591502 | Acot2 | acyl-CoA thioesterase 2 | ![]() |
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2 | 4 | ||||||
MIRT591557 | Zfp449 | zinc finger protein 449 | ![]() |
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2 | 2 | ||||||
MIRT591632 | Ubtf | upstream binding transcription factor, RNA polymerase I | ![]() |
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2 | 4 | ||||||
MIRT591952 | Ceacam1 | carcinoembryonic antigen-related cell adhesion molecule 1 | ![]() |
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2 | 2 | ||||||
MIRT591973 | Ap1ar | adaptor-related protein complex 1 associated regulatory protein | ![]() |
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2 | 2 | ||||||
MIRT592040 | Tnfrsf13c | tumor necrosis factor receptor superfamily, member 13c | ![]() |
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2 | 2 | ||||||
MIRT592091 | Smo | smoothened, frizzled class receptor | ![]() |
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2 | 2 | ||||||
MIRT592109 | Slc25a12 | solute carrier family 25 (mitochondrial carrier, Aralar), member 12 | ![]() |
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2 | 2 | ||||||
MIRT592174 | Oxtr | oxytocin receptor | ![]() |
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2 | 2 | ||||||
MIRT592217 | Map3k7 | mitogen-activated protein kinase kinase kinase 7 | ![]() |
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2 | 2 | ||||||
MIRT592224 | Magee2 | melanoma antigen, family E, 2 | ![]() |
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2 | 6 | ||||||
MIRT592250 | Lcp2 | lymphocyte cytosolic protein 2 | ![]() |
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2 | 4 | ||||||
MIRT592267 | Kcnj16 | potassium inwardly-rectifying channel, subfamily J, member 16 | ![]() |
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2 | 2 | ||||||
MIRT592390 | Trp53i11 | transformation related protein 53 inducible protein 11 | ![]() |
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2 | 6 | ||||||
MIRT592420 | Stxbp5l | syntaxin binding protein 5-like | ![]() |
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2 | 2 | ||||||
MIRT592441 | Snx12 | sorting nexin 12 | ![]() |
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2 | 2 | ||||||
MIRT592515 | Npr3 | natriuretic peptide receptor 3 | ![]() |
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2 | 2 | ||||||
MIRT592626 | Mbnl3 | muscleblind like splicing factor 3 | ![]() |
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2 | 4 | ||||||
MIRT592703 | Gfra2 | glial cell line derived neurotrophic factor family receptor alpha 2 | ![]() |
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2 | 2 | ||||||
MIRT592746 | Epas1 | endothelial PAS domain protein 1 | ![]() |
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2 | 2 | ||||||
MIRT592765 | Dmd | dystrophin, muscular dystrophy | ![]() |
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2 | 4 | ||||||
MIRT592843 | Akap2 | A kinase (PRKA) anchor protein 2 | ![]() |
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2 | 2 | ||||||
MIRT592989 | Cacna2d2 | calcium channel, voltage-dependent, alpha 2/delta subunit 2 | ![]() |
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2 | 2 | ||||||
MIRT593003 | Bend4 | BEN domain containing 4 | ![]() |
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2 | 2 | ||||||
MIRT593228 | Fndc3a | fibronectin type III domain containing 3A | ![]() |
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2 | 2 | ||||||
MIRT593415 | Neurod2 | neurogenic differentiation 2 | ![]() |
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2 | 4 | ||||||
MIRT593453 | Rab11fip1 | RAB11 family interacting protein 1 (class I) | ![]() |
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2 | 2 | ||||||
MIRT593482 | Havcr2 | hepatitis A virus cellular receptor 2 | ![]() |
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2 | 2 | ||||||
MIRT593517 | Csf2ra | colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) | ![]() |
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2 | 2 | ||||||
MIRT594037 | 2810006K23Rik | RIKEN cDNA 2810006K23 gene | ![]() |
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2 | 2 | ||||||
MIRT594129 | Fam104a | family with sequence similarity 104, member A | ![]() |
1 | 1 | |||||||
MIRT595670 | Rxrb | retinoid X receptor beta | ![]() |
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2 | 2 | ||||||
MIRT595701 | Fndc7 | fibronectin type III domain containing 7 | ![]() |
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2 | 2 | ||||||
MIRT596268 | Bnc2 | basonuclin 2 | ![]() |
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2 | 2 | ||||||
MIRT596281 | Slc6a8 | solute carrier family 6 (neurotransmitter transporter, creatine), member 8 | ![]() |
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2 | 2 | ||||||
MIRT598763 | Gpr68 | G protein-coupled receptor 68 | ![]() |
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2 | 2 | ||||||
MIRT601111 | Btrc | beta-transducin repeat containing protein | ![]() |
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2 | 2 | ||||||
MIRT601202 | Arhgef9 | CDC42 guanine nucleotide exchange factor (GEF) 9 | ![]() |
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2 | 2 | ||||||
MIRT603151 | Ubxn8 | UBX domain protein 8 | ![]() |
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2 | 2 | ||||||
MIRT603192 | Trim65 | tripartite motif-containing 65 | ![]() |
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2 | 2 | ||||||
MIRT604581 | Trim71 | tripartite motif-containing 71 | ![]() |
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2 | 2 | ||||||
MIRT605447 | St18 | suppression of tumorigenicity 18 | ![]() |
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2 | 2 | ||||||
MIRT605591 | Ncam1 | neural cell adhesion molecule 1 | ![]() |
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2 | 2 |