miRTarBase - #MIRT589358


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miRNA Infomation
miRNA namemmu-miR-1194

Gene Information
Gene Symbol Osbpl8   
Synonyms AA536976, AA536995, C730029P18Rik, D330025H14Rik, ORP-8
Description oxysterol binding protein-like 8
Transcript NM_001003717   
Other Transcripts NM_175489   
Expression
Putative miRNA Targets on Osbpl8
3'UTR of Osbpl8
(miRNA target sites are highlighted)		 
>Osbpl8|NM_001003717|3'UTR
   1 AAGTTCTCAACATTGAATCCATGAACAAGAAAGATTAGATGTGATCTGGGACCATTGTTCAAATTGGCTTGCTCTTTATT
  81 AAAGCATCACAACAAACAAAACTAGGAGATAAATGTAGAAATGTCTACCTACTTTTCACATCTTTTATCCTTAACCCAAA
 161 ACAAGAGCTACTGTGTTTGGTTACTACAATGGGCTGTGTGTTAAGTGCCAGAACACTTGTATGAGACTGTGCCTACACGT
 241 GAGTATCGTAGACCCACGTGTGTATAAAGAGCTCTTCCCTGACAGCAGTTCCTGTGTTCTACAGTGTGCTGTGACGTATT
 321 CTGATACCAGTGTTCATAACAAAGATGCCGTCACTCCTATAAATACATCTAGGGTCAACTATTGTCTTCAGAAGACCGGC
 401 ATATAACTTTCATTAGGGAATTACAGTGGGAAAATGGTAGATAATAGACATATTATGAGTAATGTCAATACATTATACTG
 481 TTAAAACCATCTCCAAGTTACCTAGTTCAGTTAGTCTTCTTAGATACACCAAGAAAAAATACGTGAGTGATCAGAATTAA
 561 TATATTGTAAGGGACAAGTAGAAAATTTTTAAGAGTTTACACAAGATTTTCTAACCCCTAGCATTTAATGTTACAGATAT
 641 AGGTGAATACATAGTTATGTGTGTGTGTATATATATATATCTCACTATTAAAGACTGGTAAGCAGCTACATGGATCTATA
 721 TGTTACAATCCCAACTTAAGCACTTGCTGAAAAGGAACGGTCAGAGGTGTTTAAGTAGGTTATGTGAAATCCTGATGATT
 801 CGGTGTTTTCTTTATGAAAAACTTTTTAGCTTTCATTTAGACACAAGAGTACTCAAGGAGTAAATAGAAGAGAAAAATGT
 881 AATATCTTACTTCAAAAGTCAAAAAAGGTGGTAAGAACAGATTGCAGTGGCCTTTAAAACATTAAAATAAAATTGCTTAA
 961 ATGTGCAATAAGTTGAGCATTGGAGAATGTCAAGTTATGAAGTTTGTCATCTCCTCCCATCATCCTGTGGCAAGCCAGTT
1041 CTGTACAAGTCTTTCTGCTCTGCTCAGGCAGATTTGTTCCTAAGAGGTTTTGCCTATTTATTCAGTTTAATAATTGTGTT
1121 TTTACATAAATATTTATTATTATCAAAAACACTTCTTTAAAAGGTAGTTAAGTTTTGAAACATCATAAAATGTAGATTGT
1201 TCAAACTACAGGTTTTAGAAGGGTTTGTTGAGTGACAACTAAGAAAGAGCTGCTGTTGGCTGCATCGGCCTTCAGCTGAG
1281 AAGAGATTGAGCTACAAGGAAAGCTAACTCTGCTGTGAGAGGACAGCTGCAGTGCTCACACTGAAAGCCTCTTCTGATGA
1361 ATACAGGTGTCCTTATGTTCCTATGAACAGCCTTTTACAGTGTGGTGTTAGCAAAACAATGCTGTATTTCATATGAAAAT
1441 ATTTTAAACTTTTAACAGCATAAGCAAGATGTACCTGTATCCAGTATTTCATTATGGAGTTTTGGGTTATATTTCATATT
1521 TGTCAATATATTGTTTGTATTCATATTCTACTACCAATATTATGCTCAACTGCCTATTCATTGATGTGATATTTTGTAAA
1601 TATTGTACAATTTGATCTTTATATGATTTTAAATTACTATTAATTTATATTATATATAACTTGATTGGTTGATCACGAAA
1681 AGTCTTTTCATTGTTAAACCCTGTAATGTAAAATTGTTGGTGACATGCCTTAGAGCTCTTTCTAAATCCTTTGTGTTGTT
1761 TGTGATCTGAACAGTGCTTTATTTGTGTTGATATCCACGACTGGAAAGGTAATTGTTGGGTTTTTTTTTTTAATTTCACT
1841 TTAGAGCTTATGTTAATGGATCCTTACCCAAGGAGTCAGAAACTAATGAACAGGGTGACTGTTTCTTATACACTAAAAGC
1921 TTATGTTCAATCAAATGCTTTTCTGATGCTTTTCGTTGTATGAGTTTCTTTCTGTGAACTGAACACAAAACTCAGGAATT
2001 GGAGGATTCATATGTAAGTCCTTTAAACAACATATAATTGAACTTGAGTTTAGCCAGTGTGTGACAGACCTCGAACAATC
2081 TGATCTAAGGGTTAACTTTCTTCATAAATGGATTCATAATGTATATGTGACAAACTGTTCCAAACTTGCTGAAAACTCAT
2161 TGTGATTTCTAGGGTTTCTGACTATGACCAGTTTTGTTTGTTTTGTTTTTCATCTATCCAAAACTTCATTTTCTCACATA
2241 AAAATTTAGATAACCACAATTATTGACACAGTTGTTAAATGTCTTTAGTTTTCTGCCATTTAGTGAGATATTGCTGGCTA
2321 AGACAAGACAATAACAGGCATGTGTGGTGTTAGTGTGCTGCAGCAATCGATTTAACTTGTAATTCCTACCACTTCTGTCT
2401 CTAGTATGACTTGGAAAGCGGCAGTGGTCATCTTTGTCACAGCTCAGTTGACAGCTGCATTCCCTTAAAAAGTTGGCCTT
2481 GTGAGATACAATCCTCATTTAATATTTCATGCTTTTGTGCCTTTAAGGAAATATTTTTGTTAGTTTTTTGTTACAGAACC
2561 ATAATGTGTTCAAAGTGTTTGTAGCTTATTTGTCATAAAAGGGTCATTTGTCTGGGTCACTGTGTTTCCTTTTATCTAGC
2641 TGAAGAATATGTAAACAGTAATGCCGTACTTTTATAAGGGTTTGTGTAGTAACTATTTCAAATATATTCATACTCAGACA
2721 TCAATGAAGTAAACTTGTCAGATGGCTCTGAGTATTGTAGACAGTGCCTTTTTGATGGAATTCACACTGTTTTCGGGTGT
2801 CAACTTGTGCCATATTCACAGAATTTTGTGGAAGGCAGTTTTAACTTTTTGAAGAATATCGTCATCAAAATTTTAAAAAA
2881 CAAATGTATAAAACTTCATCTTTTTTCAGTGTTTAGCATTTCTAGTCAGCAGTAAATAGTCTGGTTTTGCTTTTGTTTTT
2961 GTCTTATTTCAGGTATGATGGCATACGTACGCGATGAGCCATACACGAGACTGTAGACTATTACATTAAATATAAATAAA
3041 GAACATGTTCGTTTATACAAATTTTCCGATTTGACCCAGTGGGAGAGACCTTTCATAATTCAATAAGTTTACATTTCAAT
3121 GTCTTTCATATTTGACTACATATAAAGAGATTCTTTAAAATGTATAACCTGCCACCACTGCATAATTTGGTTTCATTTTG
3201 TTATGTATTGTGTGGGTTTAGTATATCCAATTTTTGTTACAATTAAAGGACTATTTTGTTACAGTTTTTAATTGAAGATG
3281 GACTGTTAACATTGTACAGGACCAGTGTCTTATTAATAAGATTAACATATTTAGTGAGAGCCACAGGAAAACCTTGACAA
3361 AAATGAATGTGACTATACTTTATGAAGTATTTCGAAAAATCAGCCTCTTTATTTATCATAAAATTATTTTAATATCATAA
3441 TATTGATGTTCTTTTTTTTTTGTTTTGTTTTGTTTTTTGTTTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTC
3521 CTGGAACTCATTCTGTAGACCAGGCTGGCCTTGAACTCAGAAATCCGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAG
3601 GTGTGTGCCACCACACCCGGCTAATATTGATAATCTTTATTTGAAAAAATGTCATGAACCATTTGAATGATGAGCCACAG
3681 AACCTCAGTTGAATTTATTTCCACTTTTGGCATGTTAAATATAGATTTAATTTTAAGTACTTCAGTTAATGGGTTTATAA
3761 AGTCAAGCACTAGCATTGGTCAGTTTTGTATGATAGGATGTAAGTGTGTTCTCACCTGCAGTGTAAATACAGCACACTGT
3841 AGAATTCTCTTAAGGTGCATAGTAAATGTATAGATAGTCACAGGCGGTTTTGTAATGTATACATTTCTAATCTATTATTC
3921 CTAACCTGTCATGTTTGCAGAGAGAAAAGAATTTTTCTAATGATCTGTAAAATTATGTTAACTTCTACAAGTAGGTATTC
4001 TAAATAAACTTTTTTAAAAGAGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM622570
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1A
Location of target site NM_175489 | 3UTR | GUUUUUUUCGAGACAGGGUUUCUCUGUGUAGCCCUGGCUGUCCUGGAACUCAUUCUGUAGAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM622571
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1B
Location of target site NM_001003717 | 3UTR | AGCCCUGGCUGUCCUGGAACUCAUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM622572
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT2
Location of target site NM_175489 | 3UTR | AGGGUUUCUCUGUGUAGCCCUGGCUGUCCUGGAACUCAUUCUGUAGACCAGGCUGGCCUUGAACUCAGAAAUCCGCCUGCCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM622573
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / KO1
Location of target site NM_001003717 | 3UTR | AGCCCUGGCUGUCCUGGAACUCAUUCUGUAGACCAGGCUGGCCUUGAACUCAGAAAUCCGCCUGCCUCUGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM622574
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / KO2
Location of target site NM_001003717 | 3UTR | AGGGUUUCUCUGUGUAGCCCUGGCUGUCCUGGAACUCAUUCUGUAGACCAGGCUGGCCUUGAACUCAGAAAUCCGCCUGCCUCUGCCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_001003717 | 3UTR | UCAUUCUGUAGACCAGGCUGGCCUUGAACUCAGAAAUCCGCCUGCCUCUGCCUCCCG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
39 mmu-miR-1194 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577399 Usp29 ubiquitin specific peptidase 29 2 2
MIRT578645 Gtf2h2 general transcription factor II H, polypeptide 2 2 4
MIRT583791 Elf1 E74-like factor 1 2 2
MIRT585587 Tox4 TOX high mobility group box family member 4 2 4
MIRT585769 Spg21 SPG21, maspardin 2 2
MIRT586369 Pctp phosphatidylcholine transfer protein 2 6
MIRT586934 H2-T24 histocompatibility 2, T region locus 24 2 4
MIRT587278 Fam154b stabilizer of axonemal microtubules 2 2 2
MIRT589358 Osbpl8 oxysterol binding protein-like 8 2 4
MIRT591418 Cd300a CD300A molecule 2 4
MIRT594881 Lpar6 lysophosphatidic acid receptor 6 2 2
MIRT594899 Hs6st2 heparan sulfate 6-O-sulfotransferase 2 2 2
MIRT595087 Unc5d unc-5 netrin receptor D 2 2
MIRT595608 Jazf1 JAZF zinc finger 1 2 2
MIRT595914 Syne2 spectrin repeat containing, nuclear envelope 2 2 2
MIRT596713 Ybey ybeY metallopeptidase 2 2
MIRT597013 Thoc6 THO complex 6 2 2
MIRT597522 Rbak RB-associated KRAB zinc finger 2 2
MIRT598050 Nip7 NIP7, nucleolar pre-rRNA processing protein 2 2
MIRT598082 Neu3 neuraminidase 3 2 2
MIRT598528 Itsn1 intersectin 1 (SH3 domain protein 1A) 2 2
MIRT598953 Ggps1 geranylgeranyl diphosphate synthase 1 2 2
MIRT598967 Gata5 GATA binding protein 5 2 2
MIRT599086 Efcab4a calcium release activated channel regulator 2B 2 2
MIRT599348 Commd7 COMM domain containing 7 2 2
MIRT599554 C3ar1 complement component 3a receptor 1 2 2
MIRT600166 Xpo7 exportin 7 2 2
MIRT600668 March5 membrane-associated ring finger (C3HC4) 5 2 2
MIRT600971 Dlc1 deleted in liver cancer 1 2 2
MIRT601455 Tnfsf10 tumor necrosis factor (ligand) superfamily, member 10 2 2
MIRT601502 Tmem88b transmembrane protein 88B 2 2
MIRT601514 Tmem151a transmembrane protein 151A 2 2
MIRT601558 T2 brachyury 2 2 2
MIRT601925 Onecut3 one cut domain, family member 3 2 2
MIRT601984 Mzt1 mitotic spindle organizing protein 1 2 2
MIRT602669 Car5b carbonic anhydrase 5b, mitochondrial 2 2
MIRT603331 Slc35d2 solute carrier family 35, member D2 2 2
MIRT605546 Ppp1r16b protein phosphatase 1, regulatory (inhibitor) subunit 16B 2 2
MIRT606291 Nrcam neuronal cell adhesion molecule 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-1 Anthocyanin NULL 145858 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Caffeic acid NULL 689043 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Catechin approved 9064 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-1 Curcumin NULL 969516 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Ferulic acid NULL 445858 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Hesperidin NULL 10621 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-1 Quercetin NULL 5280343 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Galactose NULL 6036 Quantitative real-time PCR lens 22736950 2012 up-regulated
miR-1 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-1 Trichostatin A (TSA) NULL 444732 Quantitative real-time PCR myocardial differentiation of mouse ES cells 19521018 2009 down-regulated
miR-1 Sulfonyl-hydrazone-1 (SHZ) NULL NULL Quantitative real-time PCR Murine broblast-derived Induced pluripotent stem cells 21445862 2011 up-regulated
miR-1 Cocaine NULL 446220 Next-generation sequencing ventral striatum 21708909 2011 up-regulated
miR-1 Atorvastatin approved 60823 Quantitative real-time PCR Cardiomyocyte 23860036 2013 down-regualted
miR-1 Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-1 Docosahexaenoic acid NULL 445580 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miR-1 Palmitic acid approved 985 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miR-1 17beta-estradiol (E2) approved 5757 Microarray MCF-7AKT breast cancer cells 19528081 2009 down-regulated
miR-1 Essential amino acids (EAA) NULL NULL Quantitative real-time PCR skeletal muscle of young adults 19828686 2009 up-regulated
miR-1 Hydrogen peroxide (H2O2) NULL 784 Quantitative real-time PCR Human umbilical vein endothelial cells 21527937 2011 down-regulated
miR-1 Trichostatin A (TSA) NULL 444732 Microarray apoptosis-resistant breast cancer cells 21971930 2011 up-regulated
miR-1 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-1 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated
miR-1 Bicalutamide approved 2375 Microarray prostate 22674191 2012 up-regulated
miR-1 Arsenic trioxide approved 14888 Quantitative real-time PCR cardia 22889704 2012 up-regulated
miR-1 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-1 Quinidine approved 441074 Quantitative real-time PCR myocardial infarction (MI) rats 19775284 2009 up-regulated
miR-1 Tanshinone IIA NULL 164676 Quantitative real-time PCR myocardial infarction (MI) rats 19775284 2009 down-regulated
miR-1 Tanshinone IIA NULL 164676 Quantitative real-time PCR post-infarction rat cardiomyocytes 21220930 2011 down-regulated
miR-1 Isoproterenol approved 3779 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-1 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 up-regulated
miR-1 Thiourea (TU) NULL 737139 Quantitative real-time PCR skeletal muscle and heart 22142802 2012 up-regulated
miR-1 Thiourea (TU) NULL 737139 Quantitative real-time PCR skeletal muscle and heart 22142802 2012 down-regulated
miR-1 Isoproterenol approved 3779 Quantitative real-time PCR heart 22847192 2012 up-regulated

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